• Molecules and Cells
• /
• v.42 no.6
• /
• pp.460-469
• /
• 2019

Bacterial ${\alpha}-type$ carbonic anhydrase (${\alpha}-CA$) is a zinc metalloenzyme that catalyzes the reversible and extremely rapid interconversion of carbon dioxide to bicarbonate. In this study, we report the first crystal structure of a hyperthermostable ${\alpha}-CA$ from Persephonella marina EX-H1 (pmCA) in the absence and presence of competitive inhibitor, acetazolamide. The structure reveals a compactly folded pmCA homodimer in which each monomer consists of a 10-stranded ${\beta}-sheet$ in the center. The catalytic zinc ion is coordinated by three highly conserved histidine residues with an exchangeable fourth ligand (a water molecule, a bicarbonate anion, or the sulfonamide group of acetazolamide). Together with an intramolecular disulfide bond, extensive interfacial networks of hydrogen bonds, ionic and hydrophobic interactions stabilize the dimeric structure and are likely responsible for the high thermal stability. We also identified novel binding sites for calcium ions at the crystallographic interface, which serve as molecular glue linking negatively charged and otherwise repulsive surfaces. Furthermore, this large negatively charged patch appears to further increase the thermostability at alkaline pH range via favorable charge-charge interactions between pmCA and solvent molecules. These findings may assist development of novel ${\alpha}-CAs$ with improved thermal and/or alkaline stability for applications such as $CO_2$ capture and sequestration.

• Korean Journal of Metals and Materials
• /
• v.49 no.1
• /
• pp.85-91
• /
• 2011

Alnico magnets can be used as magnetic bearings for the precise electric power measuring instruments such as watt-hour meters because they have high remanence ($B_r$), relatively high maximum energy product ($(BH)_{max}$), and excellent temperature stability. In this study, Alnico composite magnets were fabricated by appropriately mixing alnico alloy powders with epoxy resin and binder. The Alnico powders mixed with epoxy resin and a hardening agent with a mixing ratio of 96:4 were pressed and then cured to be a toroid-type ring magnet with an outer diameter (${\Phi}_{out}$) of 15 mm, an inner diameter (${\Phi}_{in}$) of 6.5 mm and a thickness (t) of 2.5 mm, respectively. The magnetic properties of the Alnico ring magnets were varied with the mixing ratio of Alnico powders that possess different average particle sizes. The Alnico ring magnet prepared by mixing 5 wt% of $50{\mu}m$ (small size) powder, 15~20 wt% of $150{\mu}m$ (medium size) powder, and 75~80 wt% of $300{\mu}m$ (large size) powder showed the best magnetic properties (remanent induction, coercive force, maximum energy product, and surface flux density). In addition, measurements of temperature and moisture characteristics for the Alnico ring magnets showed that the surface flux densities of the N and S poles decreased little and the repulsive distance between the magnets decreased as small as 0.05 mm after 10 days.

• Journal of Advanced Navigation Technology
• /
• v.25 no.3
• /
• pp.211-216
• /
• 2021

Multiple UAV System has been used for various purposes such as reconnaissance, networking and aerial photography. In such systems, it is essential to form and maintain the formation of multiple UAVs. This paper proposes the algorithm that produces an autonomous distributed control for each vehicle for a flexible formation. This command is a repulsive force in the form of the second-order system by the nearest UAV or mission area. The algorithm uses the relative position/speed through sensing and communication for calculating the command without external intervention. The command allows each UAV to follow the reference distance and fill the mission area as densely as possible without overlapping. We determine the reference distance via optimization technique solving the packing problem. The mission area comprises the desired formation outline and can be set flexibly depending on the mission. Numerical simulation is carried out to verify the performance of the proposed algorithm under a complex and flexible environment. The formation is formed in 26.94 seconds and has a packing density of 71.91%.

• The Journal of the Acoustical Society of Korea
• /
• v.41 no.6
• /
• pp.691-697
• /
• 2022

In this work, a straightforward component design of a direct current (DC) relay equipped in electric vehicles is discussed. The work aims to provide and evaluate effective measures for reducing high-frequency sound from the DC relay carrying electric power. From the operation experiments for the relay, it is observed that noise is caused by the resonance from the forced vibration by the electromagnetic repulsive force originating at the area of electric contacts with a resonance frequency of around 710 Hz ~ 730 Hz. A finite element model for the relay was established to conduct vibration mode analysis, consisting of stationary and movable contacts and a contact spring. Vibration mode analysis indicates that in the resonance frequency, the movable contact with two-point contacts experiences rotational vibration mode. For the proposed relay with a three-point contact, vibration mode analyses give reasonable results of reducing noise at that frequency. Furthermore, for the fabricated relays with the three-point contact, similar results have been obtained. In conclusion, one can see that the proposed measures provide one of the feasible solutions to the reduction of relay noise.

• Elastomers and Composites
• /
• v.37 no.4
• /
• pp.211-216
• /
• 2002

The polymer-polymer interaction parameter, x 23t, of the styrene-butadiene polymer (SBR) and ethylene-propylene-diene terpolymer (EPDM) was investigated by observing the phase behavior of the ternary system of SBR/EPDM/solvent. The solvent used in this study was benzene acting as a good solvent for SBR but as a poor solvent for EPDM. Ternary solutions with various concentrations and mixing ratios of the two component polymers were separated into two phases by temperature change The cloud point curves (CPC) showed that the differerence of solvent affinities toward each polymer and the repulsive interaction between two polymers considerably affect the shape of CPC near 15℃. In the temperature range of 5℃ ~ 25℃, incompatible behaviours arised from both the difference of mixing ratios and concentration were clearly observed. Also the phase separation temperature greatly influenced on the composition of each separated phase. The calculated x 23t values from Flory-Huggins theory were in the range of 0.6301 ~ 1.0775, which suggest that the SBR/EPDM systems are incompatible.

• Composites Research
• /
• v.12 no.4
• /
• pp.71-78
• /
• 1999

To determine the effect of chemical structure of linear amine curing agents on thermal and mechanical properties, standard epoxy resin DGEBA was cured with diaminodiphenyl methane (DDM), diaminodiphenyl sulphone (DDS) in a stoichiometrically equivalent ratio. From this work, the effect of aromatic amine curing agents. In contrast, the results show that the DGEBA/DDS cure system having the sulfone structure between the benzene rings had higher values in the conversion of epoxide, density, shrinkage (%), glass transition temperature, tensile modulus and strength, flexural modulus and strength than the DGEBA/DDM cure system having methylene structure between the benzene rings, whereas the DGEBA/DDM cure system presented higher values in the maximum exothermic temperature, thermal expansion coefficient, and thermal stability. These results are caused by the relative effects of sulfone group having strong electronegativity and methylene group having (+) repulsive property and stem from the effect of the conversion ratio of epoxide group. The result of fractography shows that the each grain size of the DDM/DGEBA system with feather-like structure is larger than that of the DDS/DGEBA system.

• Journal of the Korean Chemical Society
• /
• v.38 no.11
• /
• pp.785-791
• /
• 1994

The $Sr_1-_xY_xMnO_3$ (x = 0.0∼1.0) system was synthesized using amorphous citrate process. The stability of various structures and the electronic transport properties of this system were investigated. X-ray diffraction study indicated that the $Sr_1-_xY_xMnO_3$ system has three different structures depending on composition, namely, 4L-hexagonal perovskite (when x is less than 0.3), pseudocubic perovskite (when x is 0.3∼0.7), and hexagonal nonperovskite (when x is larger than 0.7) structures. The structural changes and electronic properties were interpreted based on two factors, i.e., the size of cations and the oxidation state of manganese ion. When the concentration of Y substitution exceeds 30%, the Mn-Mn repulsive interaction dominates over intermetallic attraction, and thus structure changes to pseudocubic perovskite. In perovskite phase the unit cell dimensions increases with increasing $Mn^{3+}$ ions due to yttrium substitution. The band gap of $Sr_{0.9}Y_{0.1}MnO_3$ is greater than that of $Sr_{0.5}Y_{0.5}MnO_3$. The greater band gap of $Sr_{0.9}Y_{0.1}MnO_3$ indicates that the 4L-hexagonal structure is more stabilized than cubic perovskite due to the Mn-Mn bond.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.31 no.12
• /
• pp.1672-1681
• /
• 2007

Cationization is effective to complement the defects of Tencel blended fabrics by introducing new functions. For this purpose, we used chitosan, which is congenial to the human body, free of pollution, and easily reacted. Then, we compared it to the Tencel single fabrics. To perform such effective cationization, the fabrics were treated with chitosan after NaOH pretreatment and enzyme treatment thereof. After that, the fabrics were treated with a crosslinking agent and a softner. The dyeing property of the cationized Tencel blended fabrics and reactive dye, which is a type of anionic dye, show a high concentration in neutral salt and excellent repulsive power between the fabrics and the decreased dyes. The dyeing property of the chitosan treated fabrics represented better performances than that of untreated fabric in the lower concentration of neutral salt. Meanwhile, when it was dyed with certain acid dyes, the dyeing property of the chitosan treated fabrics showed better results due to the reaction of an amine group, which was introduced by chitosan treatment. Thus, the verification of the cationization of the Tencel blended fabrics was performed. The washing fastness of the Tencel blended fabrics showed a little bit better than that of the Tencel single fabric, and it represented a better performance in the dye with a reactive dye than that of an acid dye.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.31 no.4
• /
• pp.307-325
• /
• 1995

IN order to gain a fundamental data for forecast or control of fish behavior and evaluated the feasibility of an application of the modeling technique to a field, in this paper a numerical model for describing the behavior of fishes in a water tank was presented. The parameters of the model were estimated by using the time-series data on the three-dimensional position of fishes and by applying the least squares algorithm. The estimated parameters were standardized to examine the variation of parameters according to the number of individuals and flow speed that the mean values of parameters were to be zero and their variances were to be one. The results obtained can be summarized as follows: (1) The standardized parameter $a^*$of propulsive force decreased according to increased the number of individuals and the flow speed. (2) The standardized parameter ${k_b}^*$ of interactive force increased according to increased the number of individuals, but decreased according to the flow speed. (3) The standardized parameter ${k_c}^*$ of schooling force increased according to │increased the number of individuals and the flow speed. (4) The standardized parameter │${k_w}^{+*}$│ of repulsive force against wall or bottom increased according to increased the number of individuals, but decreased according to the flow speed. (5) The standardized parameter │${k_w}^{-*}$│ of attractive force against wall or bottom was generally constant according to increased the number of individuals, but increased according to the flow speed. (6) The standardized parameter $\upsilon$ super(*) of damping force increased according to increased the number of individuals, but decreased according to the flow speed.

• Journal of Korean Society of Transportation
• /
• v.34 no.5
• /
• pp.409-420
• /
• 2016

Korea is expected to enter 'Super Aged Society' in 2026. However, as walking is the very basic human right of mobility, securing safe and convenient moving of elderly people comprising the majority of transportation vulnerable is thought to be the most basic welfare, which can be easily neglected. From this perspectives, this study provides the walking characteristics of elderly people to be used in design of pedestrian facilities. The analysis of the measurements using Motion Analysis Systems shows that all walking factors of elderly people is 75% level of the younger group. Elderly group shows slower movement, reduced shoulder movement and increased ankle movement compared to the others. Also, foots are risen less and ground repulsive force is increased. Cluster analysis shows that the group of the elderly shows high variability inside the group, and 2 or 3 clusters can be formed with factors of Walking, Balance and Muscles. These walking characteristics can be used in designing pedestrian road, slope and step height of roadway facilities.