• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.3
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• pp.239-246
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• 2003

We have examined the 16S-23S rRNA intergenic spacer region (ISR) of Vibrio vulnificus KCTC 2959. ISRs were amplified by primers complementary to conserved regions of 16S and 23S rRNA genes. ISR amplicons were cloned and sequenced. Analysis of the ISR sequences showed that V. vulnificus KCTC 2959 contains five types of polymorphic ISRs. Size of ISRs ranged from 424 to 741 bp in length and the number of tRNA genes ranged from one to four. The ISRs were designated as ISR-E $(tRNA^{Glu}),\;ISR-IA\;(tRNA^{Ile}-tRNA^{Ala})$, ISR-EKV $(tRNA^{Glu}-tRNA^{Lys}-tRNA^{Val})$, ISR-IAV $(tRNA^{Ile}-tRNA^{Ala}-tRNA^{val})$ and ISR-EKAV $(tRNA^{Glu}-tRNA^{Lys}-tRNA^{Ala}-tRNA^{Val})$ based on their tRNA genes. Multiple alignment of representative sequences from different Vibrio species revealed several domains of high sequence variability. We used the sequences of variable domains to design species-specific primer for detection PCR. Specificity of the primers was examined using genomic DNA prepared from 18 different Vibrio species. The results showed that the PCR using primers designed in this study can be used to detect V. vulnificus from other Vibrio species.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.117-124
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• 2005

Two bacterial isolates obtained from rotifer and diseased olive flounder larvae, Paralichthys olivaceus, were identified as Vibrio ichthyoenteri based on the results of phenotypic characterization. In an attempt to develop rapid PCR method for the detection of V. ichthyoenteri, we examined the 16S-23S rRNA intergenic spacer region(ISR) of V. ichthyoenteri and developed species-specific primer for V. ichthyoenteri. Analysis of the ISR sequences showed that V. ichthyoenteri contains one type of polymorphic ISRs. The size of ISRs was 348 bp length and did not contain tRNA genes. Mutiple alignment of representative sequences from different V. species revealed several domains of high sequence variability, and allowed to design species-specific primer for detection of V. ichthyoenteri. The specificity of the primer was examined using genomic DNA prepared from 19 different V. species, isolated 18group Vibrio species and most similar sequence of other known Vibrio species. The results showed that the PCR reaction using species-specific primer designed in this study can be used to detect V. ichthyoenteri.

• Journal of the Korean association of regional geographers
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• v.23 no.2
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• pp.257-268
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• 2017

The three cities, Yeosu, Suncheon, and Gwangyang, in located in Gwangyang Bay which functions as a representative coastal industrial area in Korea, form a single functional area of living, municipality, and economy. In this regional context, some local merchants and scholars are promoting a inter-municipal cooperation of three cities. This study analyzes how the group of specialists perceives and evaluates the inter-municipal cooperation of three cities. The group of specialists highly evaluate the potential of future development of Gwangyang Bay region, and the relevance and necessity of feasibility of inter-municipal cooperation of three cities. It is estimated that the inter-municipal cooperation of three cities would need 8.3 years for its full-fledged shape. In order to accomplish the inter-municipal cooperation, the sharing the development vision of Gwangyang Bay region and the understanding and consent of the residents for the inter-municipal cooperation are suggested as the most important factors.

• Land and Housing Review
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• v.2 no.1
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• pp.1-8
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• 2011

During the 1990's, five new towns were constructed around Seoul such as Bun-Dang and Il-San. These new towns are very popular since they were created based on systemic urban planning and they provide various residential amenities. Many people consider them livable cities. Taking such advantages, other small-scale new public and private developments continued around those new towns and consequently created 'agglomerated region with new development'. The most representative area is Bun-Dang and north-western Yong-In area which are formed along the Seoul-Busan expressway. This area is a mega-community having more than one million residents in 2005.Judging from recent development trends in the Seoul metropolitan area, it is expected that the formation of other 'agglomerated region with new development' would be continued for the time being. This study is aimed to analyze the residential environment of Bun-Dang and north-western Yong-In and find implications to the management of 'agglomerated region with new development'. The relevant existing studies have their limits, only focusing on analyzing current conditions and pointing out current problems. This study could be differentiated from the existing analyses in that it conducted comprehensive analyses on various factors that constitute urban residential environment. The conclusion of this study was derived from diverse analyses of current development condition, residential sites, regional transportation, awareness of residents, and oversee cases.

• Journal of Digital Convergence
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• v.15 no.12
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• pp.13-23
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• 2017

Obviously, "informatization" is one of the most effective means for improving national competitiveness. In particular, South Korea is a representative successful case implementing informatization in the world. On the contrary, North Kora does not because of serious information devide matter. Given the assumption that information devide is caused by three aspects: the access to information devices or database(information accessibility); the ability to utilize information resources(information mobilization); and the ability to differentiate the quality of information(information consciousness). Based upon three aspects of information devide, we design practical strategies to implement informatization for North Korea region. The purpose of this study is to investigate how to successfully build in informatization of North Korea region for unification era. Drawing on the analysis of successful cases of South Korea, we suggest the valuable guidelines and directions toward success of building informatization of North Korea region. Therefore, this study provides government with specific and practical success strategies for unification era.

• Korean Journal of Environment and Ecology
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• v.18 no.2
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• pp.107-123
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• 2004

The vascular plants of Mt. Deog-yu area in the Baekdudaegan were recorded as 411 taxa; 91 families, 243 genera, 353 species, 53 varieties and 5 forma. From the floristic point of view, the Baekdudaegan, a main mountain range of the Korean peninsula, in the region of Mt. Deog-yu, belongs to the southern province in Korea, as the species like Cephalotaxus koreana, Lindera obtusiloba, Ilex macropoda and Stewartia koreana. are ranged in this region. But the species of the Northern element such as Betula costata, Betula ermani, Heloniopsis orientalis and Disporum ovale are also recorded in the region. Above the sea level from 1,000m, many sub alpine plant species are recorded. Rare and endangered plant species which are categorized by the Office of Forestry were 10 species such as Lilium cernum, Paeonia obovata, Abies koreana, Disporum ovale, Lilium distichum, Tricyrtis dilatata, Clematis chiisanensis, Bupleurum euphorbioides, Rhododendron schonoskii and Iris ensata var. spontanea. The protected plant species which are categorized by the Ministry of Environment were recorded only Lilium cernum. The Korean endemic plant species were recorded as 22 taxa; 12 families, 21 genera, 20 species 1 varieties, and 1 forma. Among them the plant species such as Cephalotaxus koreana, Abies koreana, Hosta capitata, Polygonatum lasianthum var. coreanum and Tricyrtis dilatata are representative ones in the region.

• Korean Journal of Veterinary Research
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• v.22 no.1
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• pp.53-58
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• 1982

Stomachs from 3,354 fattening pigs were examined at slaughter during the period from September 1980 to August 1981. Pigs of both sexes and $Landrace{\times}Hampshire$ crossbreds were included in the present studies, and they weighed about 60-120kg. Gross pathologic alterations of the stomach were classified as normal, epitnelial change, erosion, ulcer and scar formation. Representative tissue sections were taken from the stomach lesions at random and fixed in 10% buffered formalin. Cut sections were stained with hematoxylin and eosin, and examined histopathologically. The results obtained in the present studies were as follows. 1. In the seasonal prevalence of gastric ulceration, severe ulceration with erosion was shown during the Autumn and Winter, whereas mild ulceration was mainly shown during the Spring and Summer. 2. Of the 3,354 stomachs of the pigs, 20.8% were found to be normal. Of the rest, 40% had epithelial changes, followed by erosion (24.7%), ulcer (13.6%) and scar formation (0.9%), respectively. 3. In the prevalence of ulcers in the different regions of the stomach, the fundic region had the highest rate (61.6%) of ulceration, followed by cardiac (21.7%), esophageal (15.0%) and pyloric region (1.7%). 4. The principal gross changes were severe epithelial changes with keratotic proliferation in the esophageal region, and in the fundic region severe folding of the stomach wall was covered with bloody mucous exudates. 5. Main histopathological changes were inflammatory cell infiltrations in most cases, hemorrhages in acute ulcers and prominent proliferation of granulation tissues in chronic ulcers.

• Korean Journal of Environmental Agriculture
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• v.38 no.1
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• pp.1-9
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• 2019

BACKGROUND: Accurate and simple analytical method determining Fluxametamid residue was necessary in various food matrices. Additionally, fulfilment of the international guideline of Codex (Codex Alimentarius Commission CAC/GL 40) was required for the analytical method. In this study, we developed Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method to determine the Fluxametamid residue in foods. METHODS AND RESULTS: Fluxametamid was extracted with acetonitrile, partitioned and concentrated with dichloromethane. To remove the interferences, silica SPE cartridge was used before LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) analysis with $C_{18}$ column. Five agricultural commodities (mandarin, potato, soybean, hulled rice, and red pepper) were used as a group representative to verify the method. The liner matrix-matched calibration curves were confirmed with coefficient of determination ($r^2$) greater than 0.99 at calibration range of 0.001-0.25 mg/kg. The limits of detection and quantification were 0.001 and 0.005 mg/kg, respectively. Mean average accuracies were shown to be 82.24-115.27%. The precision was also shown to be less than 10% for all five samples. CONCLUSION: The method investigated in this study was suitable to the Codex guideline for the residue analysis. Thus, this method can be useful for determining the residue in various food matrices as routine analysis.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.116-116
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• 2020

Gilgit-Baltistan (GB) is a highly mountainous and remote region covering 45% of Upper Indus Basin (UIB) with around 1.8 million population is vulnerable to climate change and socio-economic growth makes water resources management and planning more complex. To understand the water scarcity in the region this study is carried out to project water supply and demand for agricultural and domestic sector under various climate-socio-economic scenarios in five sub catchments of GB i.e., Astore, Gilgit, Hunza, Shigar and Shyok for a period of 2015 to 2050 using Water Evaluation and Planning (WEAP) model. For climate change scenario ensembled mean of three global climate models (GCMs) was used under three different Representative Concentration Pathway (RCP) scenarios (RCP2.6, RCP6.0 and RCP8.5). The Shared Socioeconomic Pathways (SSPs) and agricultural Land Development (LD) scenarios were combined with climate scenarios to develop climate-socio-economic scenario. Our results indicate that the climate change and socio-economic growth would create a gap between supply and demand of water in the region, with socio-economic growth (e.g. agricultural and population) as dominant external factor that would reduce food production and increase poverty level in the region. Among five catchments only Astore and Gilgit will face shortfall of water while Shyoke would face shortfall of water only under agricultural growth scenarios. We also observed that the shortfall of water in response to climate-socio-economic scenarios is totally different over two water deficient catchments due to its demography and geography. Finally, to help policy makers in developing regional water resources and management policies we classified five sub catchments of UIB according to its water deficiency level.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.5
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• pp.1026-1033
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• 2009

In this study, we computed the unit load of nonpoint source for the forest, agricultural, and urban representative region in Anseongchun watershed. In addition, Flow-weighted mean concentration (FWMC) that well represents runoff characteristics of storm water during rainfall, was calculated, and runoff pollutants loading was also examined. FWMCs of 1st rainfall, which runoff coefficient was high, had a tendency higher than those of 2nd rainfall. Based on landuse results, pollutant concentration of the non-urban such as forest and agricultural regions was higher than that of urban region. In case of BOD, runoff pollutants loading was calculated as 1,395, 1,623, 2,268kg/d in 1st rainfall for forest, agricultural, and urban regions, respectively, while runoff loading of 2nd rainfall was 503kg/d in forest), 512kg/d in agricultural, and 898kg/d in urban. By landuses, unit load of the urban as 72.7kg/ha/yr was 12 times higher than that of the agricultural as 6.5kg/ha/yr, and 8 times higher than that of the forest as 9.5kg/ha/yr.