한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제36권3호
  • /
  • Pages.239-246
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  • 2003
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
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Vibrio vulnificus의 16S-23S rRNA Intergenic Spacer Region 분석

Analysis of 16S-23S rRNA Intergenic Spacer Region of Vibrio vulnificus

  • 박영미 (제주대학교 해양과학대학 해양과학부) ;
  • 이제희 (제주대학교 해양과학대학 해양과학부)
  • PARK Young Mi (Department of Marine Biotechnology Cheju University) ;
  • LEE Jehee (Department of Marine Biotechnology Cheju University)
  • 발행 : 2003.06.01
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초록

We have examined the 16S-23S rRNA intergenic spacer region (ISR) of Vibrio vulnificus KCTC 2959. ISRs were amplified by primers complementary to conserved regions of 16S and 23S rRNA genes. ISR amplicons were cloned and sequenced. Analysis of the ISR sequences showed that V. vulnificus KCTC 2959 contains five types of polymorphic ISRs. Size of ISRs ranged from 424 to 741 bp in length and the number of tRNA genes ranged from one to four. The ISRs were designated as ISR-E $(tRNA^{Glu}),\;ISR-IA\;(tRNA^{Ile}-tRNA^{Ala})$, ISR-EKV $(tRNA^{Glu}-tRNA^{Lys}-tRNA^{Val})$, ISR-IAV $(tRNA^{Ile}-tRNA^{Ala}-tRNA^{val})$ and ISR-EKAV $(tRNA^{Glu}-tRNA^{Lys}-tRNA^{Ala}-tRNA^{Val})$ based on their tRNA genes. Multiple alignment of representative sequences from different Vibrio species revealed several domains of high sequence variability. We used the sequences of variable domains to design species-specific primer for detection PCR. Specificity of the primers was examined using genomic DNA prepared from 18 different Vibrio species. The results showed that the PCR using primers designed in this study can be used to detect V. vulnificus from other Vibrio species.

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