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Molecular and functional characterization of a Brmecp gene encoding 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Brassica rapa (배추 유래 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 관련 Brmecp 유전자의 발현 및 분자적 특성)

  • Jung, Yu-Jin;Choi, Jang-Sun;Sun, Ju-Nam;Nou, Ill-Sup;Cho, Yong-Gu;Kang, Kwon-Kyoo
    • Journal of Plant Biotechnology
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    • v.39 no.3
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    • pp.189-195
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    • 2012
  • In plants, the fifth step of the plastidial 2-Cmethyl-D-erythritol 4-phosphate (MEP) pathway is catalyzed by 2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECP; EC: 4. 6. 1. 12), an enzyme proposed to play a key role in the regulation of isoprenoid biosynthesis. Here we report the isolation and functional characterization of a 823 bp Brassica rapa MECP (Brmecp) cDNA encoding a deduced polypeptide of 230 amino acid residues. Transcription levels of Brmecp were two-fold higher in petal compared to leaves. In addition, Brmecp expression in cabbage seedlings treated with ABA, $H_2O_2$ and drought was higher than control seedlings. These results were consistent with changes in chlorophyll contents in transgenic Arabidopsis. Thus, the Brmecp may contribute to the production of primary (chlorophylls and carotenoids) isoprenoid end-products in chloroplasts.

Congenital Bochdalek hernia: report of 4 cases (선천성 Bochdalek hernia4례 보고)

  • Jin, Jae-Gwon;Park, Ju-Cheol;Yu, Se-Yeong
    • Journal of Chest Surgery
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    • v.15 no.4
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    • pp.432-439
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    • 1982
  • Congenital posterolateral diaphragmatic hernia [Bochdalek hernia] is the result of a congenital diaphragmatic defect in the posterior costal part of the diaphragm in the region of the tenth and eleventh ribs. There is usually free communication between the thoracic and abdominal cavities. The defect is most commonly found on the left [90%], but may occurs on the right, where the liver often prevents detection. The male to female ratio is 2:1. Owing to the negative intrathoracic pressure, herniation of abdominal contents through the defects occurs, with resultant collapse of the lung. Shifting of mediastinum to the opposite side and compression of the opposite lung occurs. Most often these hernias are manifestated by acute respiratory distress in the newborn. A second, but less well recognized, group of patient with Bochdalek hernia survive beyond the neonatal period, usually present at a later time with "failure of thrive, intermittent vomiting, or progressive respiratory difficulty. " The diagnosis can often be made on clinical ground from the presence of respiratory distress, absence of breath sounds on the chest presence of bowel sounds over the chest . Roentgenogram of the chest confirm the diagnosis. Obstruction and strangulation have been reported but are rare. Treatment consists of early reliable identification of these congenital diaphragmatic hernia with high risk and surgical repairment. and postoperative pharmacological management with extracorporeal membranous oxygenation [=ECMO] support in the period of intensive care. On the surgical approach, for defects on left side, an abdominal incision is preferred, because of the high incidence of malrotation and obstructing duodenal bands. In the neonate, the operative mortality may be appreciable, but, later repair almost always is successful. During the period from 1972 to 1982, 4 cases of congenital Bochdalek hernia were experienced at the Kyung-Hee University Hospital.

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Analysis of polyphenolic metabolites from Artemisia gmelinii Weber ex Stechm. and regional comparison in Korea

  • Park, Mi Hyeon;Kim, Doo-Young;Jang, Hyun-Jae;Jo, Yang Hee;Jeong, Jin Tae;Lee, Dae Young;Baek, Nam-In;Ryu, Hyung Won;Oh, Sei-Ryang
    • Journal of Applied Biological Chemistry
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    • v.62 no.4
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    • pp.433-439
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    • 2019
  • Artemisia species are widely used as food ingredients and raw material in traditional medicine. However, to date, the secondary metabolites of Artemisia gmelinii Weber ex Stechm. have not been sufficiently investigated. The secondary metabolites of A. gmelinii, which was collected from representative regions in Chungbuk, Gangwon, and Gyeongbuk, were analyzed using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTof MS) combined with an unsupervised principal component analysis (PCA) multivariate analysis. In the loading scatter plot of PCA, significant changes in metabolites were observed between the regions, ten metabolites (3: 5-O-caffeoylquinic acid, 4: 4-O-caffeoylquinic acid, 8: trans-melilotoside, 12: quercetin 3-O-hexoside, 15: 3,4-O-dicaffeoylquinic acid, 17: 3,5-O-dicaffeoylquinic acid, 18: 4,5-O-dicaffeoylquinic acid, 19: syringaldehyde, 20: caffeoylquinic acid derivative, and 23: icariside II) were evaluated as key markers among twenty-five identified metabolites. Interestingly, the contents of the identified marker significantly differed between the three groups. This is the first study to report the presence of marker metabolites and their correlating geographical cultivation in A. gmelinii.

A Study on The Manufacturing Industries of Women's Wear in Taegu Through the Sewing Technicians (봉제기술자(縫製技術者)를 통해서 본 대구시(大邱市) 숙녀복업계(淑女服業界)의 현항(現況))

  • Kim,, Hyo-Eun;Kim, Soon-Boon
    • Journal of Fashion Business
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    • v.2 no.4
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    • pp.102-111
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    • 1998
  • The purpose of this study is to present the basic material for the competitive, high-quality products by analyzing employees' attitude toward their job and some factors which influence the enhancement of productivity, education of workers, and facilities. This project was mainly conducted the conditions of women's wear manufacturing industries in Taegu by interviewing 143 employees in the sewing department. The result of this dissertation can be summarized as follows; 1. When it comes to the division of task in the sewing department, one team is composed of chairpersons (33.5%), assistant members (27.4%) and sub-assistant members (26.6%). The inspection of commodities as well as the enhancement of work on ironing for elaboration was divided as completion (12.6%) and finally the forms of task were made up of design sampling team (49%) and contracting team (51%). 2. Among the needle workers, as many as 60% employees had received technical education, which was by means of being passed down from the predecessors (81.6%). In terms of the period of education, from 2 to 5 years topped the list. The contents of education comprise patterning, sewing (65.2%) and ironing (20.3%). 3. The department of design takes charge of the report on working directions up to 88.2% and the working directions were used by 69.9 % of technicians. The directors of working conditions and methods were chiefs of designing department (37.7%) and those of sewing department (30.8%). The factors of defective goods were low-quality materials (50.0%), the deficiency in skills of workers. In terms of methods for preventing defects, technical education of workers, standardization of task and investment for factory automation were suggested. 4. As for their perception of present work, most of employees (80%) look upon it as a way of earning a living and 11% of them wanted to derive many things from their work. 59.2% of workers were satisfied with the product. When it comes to the incentive system provided by company, 67.0% responsed that it was not bad, and 23.9% evaluated it as 'satisfactory'.

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The Costume and the thought to Costume of the Ambassador Extraordinary(修信使) and the Inspectors(朝士視察團) Detached to Japan before the Reform of Dress Regulation in 1884(甲申衣制改革) (갑신의제개혁(1884년) 이전 일본 파견 수신사와 조사시찰단 복식 및 복식관)

  • Lee, Kyung-Mee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.33 no.1
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    • pp.45-54
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    • 2009
  • The reform of dress regulation in Kapshin(甲申衣制改革; 1884) was the first dress renovation in 8 years after Chosun's port opening in 1876, and the key contents of that reform was the simplification of the formal dress and private dress. The reform in the culture of the traditional costume should require some kind of special experience because that culture had been regarded as the precious symbol of the Confucianism. The purpose of this study is to investigate the background of "the reform" and who proposed "that reform", by contemplating the costume and the thought of costume of the ambassador extraordinary(修信使) and the Inspectors detached to Japan(朝士視察團) who experienced new costume system of Japan sent by Chosun Dynasty. For this study, historical documents such as 'Sillok(實錄)', a sort of report("修信使記錄", "聞見事件") were reviewed and the evidential photos in Japan were analyzed. It can be summarized as follows. First, Kim Kisu(金綺秀, Susinsa) in 1876 and Kim Hongjip(金弘集, Susinsa) in 1880 wore the traditional costume of Chosun in Japan, and described the westernized Japanese costume in view of traditional costume culture. Second, the inspectors detached to Japan in 1881 showed the same attitude to the Japanese costume as the previous Susinsas had done. Third, Park Younghyo(朴泳孝), who was an ambassador extraordinary and plenipotentiary(特命全權大使) in 1882, experienced western style in Japan and played an positive diplomatic activities with western nations. It could be guessed that those changes in the attitudes of the diplomats might have the relationship with the change of Chosun, which began a treaty of amity between western nations after 1882. Afterwards, Chosun seems to have decided to reform the system of traditional costume into the simplified one in 1884, proposed by the diplomats who experienced foreign culture abroad including Park Younghyo(朴泳孝) and the mutual agreement in Chosun Government.

Study on Event Reporting Service for Digital Contents on the Convergence Network of Broadcasting and Communication (방송${\cdot}$통신 융합망에서의 디지털 콘텐츠 서비스를 위한 이벤트 리포팅 시스템 연구)

  • Ji Kyunghee;Moon Nammee;Kim Jaegon
    • Journal of Broadcast Engineering
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    • v.10 no.2
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    • pp.190-201
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    • 2005
  • MPEG-21 has identified the need fur Event Reporting in its vision for an interoperable multimedia framework. Event Reporting is required within the MPEG-21 multimedia framework in order to provide a standardized means for sharing information about Events amongst Peers and Users. Such Events relate to Digital Items and/or Peers that interact with them. Arising from an each Event, there is the opportunity to describe what occurred. However, there are a number of difficulties in providing an accurate report about an Event. Different observers of the Event may have vastly different perspectives, needs, and focuses. They may emphasize certain elements to the detriment of others, or they may describe an Event in a way that others may find confusing. In this paper, we design how to express Event Reporting message structure and system architecture in compliance with ISO/IEC 21000-15. And we implement the system for MPEG-21 Event Reporting service.

The Normative Meaning of Cybersecurity Information Sharing Act(CISA) of 2015 (미국 사이버안보 정보공유법(CISA)의 규범적 의의)

  • Park, Sangdon
    • Convergence Security Journal
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    • v.17 no.1
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    • pp.45-52
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    • 2017
  • The Cybersecurity Information Sharing Act(CISA) of 2015, enacted in December 2015, is one of the greatest achievements of cybersecurity legislation in the United States. The promotion of cybersecurity information sharing is one of the tasks to improve cybersecurity governance in Korea. So it is an important issue to be addressed in cybersecurity legislation in Korea in the near future. CISA has many implications for cybersecurity legislation in Korea. Nevertheless, it is difficult to find preceding research that explain the content of CISA and study its normative meaning in Korea. Therefore, in this paper, the contents of the CISA is identified and its normative meaning and implication is found in five categories: definition of terms, establishment of information sharing procedures and conditions, promotion of voluntary information sharing by the private sector, checks on the executive branch and report to the Congress, and other matters. CISA facilitates information sharing based on willingness, while eliminating the side effects that may arise in the information sharing process. It is necessary to appropriately apply the good points of CISA to the cybersecurity legal system in Korea.

A Statistical Approach about Documents of Women's recognition on the Date Rape (데이트 강간의 여성 인식에 관한 문헌통계학적 접근)

  • Park, Jong-Ryeol;Noe, Sang-Ouk
    • Journal of the Korea Society of Computer and Information
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    • v.13 no.6
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    • pp.287-294
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    • 2008
  • As shown in many precedent studies of sex violation, many rapes are done by acquaintances rather than strangers. Among them specially many are the cases carried out by date partners and so it is necessary to study on the date rape systematically and academically which is the most common sex violation. A recent study on the date rape executed in the country shows the seriousness of the problem clearly. The survey reported that 20.5 percentages of women had experienced the harmfulness of date rape. However compared with the general injured women, the injured ones of date rape rarely report it to related organs. The reason is that in the case of date rape both the injured ones and offenders just think of the injured things or offending works as a little excessive sexual relation or sexual contact, but not recognize it as sex violation. Therefore this study will introduce the results of precedent studies on the date rape based on the contents of Michael Shively, Perceived Risks of Date Rape, Judith M. Sgarzi, Victimology, and also introduce the prevention of the injury its countermeasures.

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Antioxidative and anti-inflammatory activities of Phaseolus aureus

  • Hong, In-Taik;Kim, Beom-Jun;Yu, Duck-Cho;Kim, Jung-Haeng;Kim, Jung-Han;Heo, Moon-Young;Lee, Song-Jin;Kim, Hyun-Pyo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.22 no.2
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    • pp.41-51
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    • 1996
  • Phaseolus aureus (mung bean), Leguminosae, has been used as an antidote from the ancient time. Especially, it has been widely used for cleaning face and skin in oriental countries. Although several constituents such as fatty acids, phytoallexin and phaseol derivatives were reported in P. aureus and related species including seedlings, there has been a few report to describe its biological activity. Therefore, in this investigation, the ethanol extract from P. aureus was obtained and its biological activities including the antioxidative and anti-inflammatory activities were studied. The 70% ethanol extract from P. aureus showed dose-dependent antioxidative activity (52.3% inhibition at 4 mg/ml) against lipid peroxidation assay, while the extract did not show the inhibitory activity of superoxide radical formation. The extract also showed the topical anti-inflammatory activity against croton-oil and arachidonic acid induced mouse ear edema test (18-19% inhibition at 7.5 mg/ear) as well as mild inhibitory activity against picryl chloride induced delayed hypersensitivity in mouse. For investigating active principles, vitexin and isovitexin (apigenin C-glycoside) as flavonoids, and adenosine were isolated from the extract using silica gel chromatography. The actual contents of vitexin and isovitexin were found to be 3.7 and 2.4 mg/g extract, respectively. Vitexin and isovitexin showed the antioxidative activity. They showed the topical anti-inflammatory activity, although the activities were not potent compared to the reference compounds. These results suggested that vitexin and isovitexin may be, at least in part, the compounds contributing the antioxidative activity in vitro and the topical anti-inflammatory activity of P. aureus in vivo. All results of present study might be one of the scientific rationale in using mung bean for skin care from the ancient time.

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Electrospun Non-Directional Zinc Oxide Nanofibers as Nitrogen Monoxide Gas Sensor (전기방사법에 의해 합성된 무방향성 산화아연 나노섬유의 일산화질소 가스 감지 특성)

  • Kim, Ok-Kil;Kim, Hyojin;Kim, Dojin
    • Korean Journal of Materials Research
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    • v.22 no.11
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    • pp.609-614
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    • 2012
  • We report on the NO gas sensing properties of non-directional ZnO nanofibers synthesized using a typical electrospinning technique. These non-directional ZnO nanofibers were electrospun on an $SiO_2$/Si substrate from a solution containing poly vinyl alcohol (PVA) and zinc nitrate hexahydrate dissolved in distilled water. Calcination processing of the ZnO/PVA composite nanofibers resulted in a random network of polycrystalline ZnO nanofibers of 50 nm to 100 nm in diameter. The diameter of the nanofibers was found to depend primarily on the solution viscosity; a proper viscosity was maintained by adding PVA to fabricate uniform ZnO nanofibers. Microstructural measurements using scanning electron microscopy revealed that our synthesized ZnO nanofibers after calcination had coarser surface morphology than those before calcination, indicating that the calcination processing was sufficient to remove organic contents. From the gas sensing response measurements for various NO gas concentrations in dry air at several working temperatures, it was found that gas sensors based on electrospun ZnO nanofibers showed quite good responses, exhibiting a maximum sensitivity to NO gas in dry air at an operating temperature of $200^{\circ}C$. In particular, the non-directional electrospun ZnO nanofiber gas sensors were found to have a good NO gas detection limit of sub-ppm levels in dry air. These results illustrate that non-directional electrospun ZnO nanofibers are promising for use in low-cost, high-performance practical NO gas sensors.