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http://dx.doi.org/10.5010/JPB.2012.39.3.189

Molecular and functional characterization of a Brmecp gene encoding 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Brassica rapa  

Jung, Yu-Jin (Institute of Genetic Engineering, Hankyong National University)
Choi, Jang-Sun (National Institute of Horticultural & Herbal Science)
Sun, Ju-Nam (Department of Horticulture, Hankyong National University)
Nou, Ill-Sup (Department of Horticulture, Sunchon National University)
Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
Kang, Kwon-Kyoo (Institute of Genetic Engineering, Hankyong National University)
Publication Information
Journal of Plant Biotechnology / v.39, no.3, 2012 , pp. 189-195 More about this Journal
Abstract
In plants, the fifth step of the plastidial 2-Cmethyl-D-erythritol 4-phosphate (MEP) pathway is catalyzed by 2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECP; EC: 4. 6. 1. 12), an enzyme proposed to play a key role in the regulation of isoprenoid biosynthesis. Here we report the isolation and functional characterization of a 823 bp Brassica rapa MECP (Brmecp) cDNA encoding a deduced polypeptide of 230 amino acid residues. Transcription levels of Brmecp were two-fold higher in petal compared to leaves. In addition, Brmecp expression in cabbage seedlings treated with ABA, $H_2O_2$ and drought was higher than control seedlings. These results were consistent with changes in chlorophyll contents in transgenic Arabidopsis. Thus, the Brmecp may contribute to the production of primary (chlorophylls and carotenoids) isoprenoid end-products in chloroplasts.
Keywords
Brassica rapa; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; isoprenoid biosynthesis; 2-C-methyl-Derythritol 4-phosphate pathway; transgenic Arabidopsis;
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