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http://dx.doi.org/10.3839/jabc.2019.059

Analysis of polyphenolic metabolites from Artemisia gmelinii Weber ex Stechm. and regional comparison in Korea  

Park, Mi Hyeon (Natural Medicine Research Center, KRIBB)
Kim, Doo-Young (Natural Medicine Research Center, KRIBB)
Jang, Hyun-Jae (Natural Medicine Research Center, KRIBB)
Jo, Yang Hee (Natural Medicine Research Center, KRIBB)
Jeong, Jin Tae (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Dae Young (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Baek, Nam-In (Graduate School of Biotechnology, Kyung Hee University)
Ryu, Hyung Won (Natural Medicine Research Center, KRIBB)
Oh, Sei-Ryang (Natural Medicine Research Center, KRIBB)
Publication Information
Journal of Applied Biological Chemistry / v.62, no.4, 2019 , pp. 433-439 More about this Journal
Abstract
Artemisia species are widely used as food ingredients and raw material in traditional medicine. However, to date, the secondary metabolites of Artemisia gmelinii Weber ex Stechm. have not been sufficiently investigated. The secondary metabolites of A. gmelinii, which was collected from representative regions in Chungbuk, Gangwon, and Gyeongbuk, were analyzed using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTof MS) combined with an unsupervised principal component analysis (PCA) multivariate analysis. In the loading scatter plot of PCA, significant changes in metabolites were observed between the regions, ten metabolites (3: 5-O-caffeoylquinic acid, 4: 4-O-caffeoylquinic acid, 8: trans-melilotoside, 12: quercetin 3-O-hexoside, 15: 3,4-O-dicaffeoylquinic acid, 17: 3,5-O-dicaffeoylquinic acid, 18: 4,5-O-dicaffeoylquinic acid, 19: syringaldehyde, 20: caffeoylquinic acid derivative, and 23: icariside II) were evaluated as key markers among twenty-five identified metabolites. Interestingly, the contents of the identified marker significantly differed between the three groups. This is the first study to report the presence of marker metabolites and their correlating geographical cultivation in A. gmelinii.
Keywords
Artemisia gmelinii; Caffeoylquinic acid; Metabolites; Region; Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry;
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