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Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae

  • Sadat, Md. Abu;Jeon, Junhyun;Mir, Albely Afifa;Kim, Seongbeom;Choi, Jaeyoung;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.30 no.4
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    • pp.367-374
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    • 2014
  • Genomes contain a large number of unique genes which have not been found in other species. Although the origin of such "orphan" genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes formed during the evolutionary history of M. oryzae. Synteny analyses using closely related fungal species corroborated the notion that these genes evolved de novo in the M. oryzae genome. In this report, we discuss our inability to detect phenotypic changes in the four deletion mutants. Based on these results, the four orphan genes may be products of de novo gene birth processes, and their adaptive potential is in the course of being tested for retention or extinction through natural selection.

A Study on the Process in MMA/IPMI/Styrene Terpolymerization (MMA/IPMI/Styrene 삼원공중합 공정연구)

  • Park Jong-Kyoung;Yoon Sung-Cheol;Jin Jung-Il;Lee Chang-Jin
    • Polymer(Korea)
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    • v.30 no.4
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    • pp.326-331
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    • 2006
  • MMA(methyl methacrylate)/IPMI(N-isopropyl maleimide) copolymers are one of the well known heat resistant materials for POF(plastic optical fiber). However, because of the large difference in the reactivity ratio between MMA and IPMI$(r_1/r_2=1.72:0.17)$, the compositional drift occurs during the polymerization process which causes the deterioration of the physical properties of these copolymers. In this paper, we report that the compositional drift of the copolymer could be reduced by the addition of styrene (St) which increased the reactivity of IPMI in the MMA/IPMI copolymerization system and conversion was also increased by 1.5 or 2 times. The MMA/IPMI/St terpolymer had higher refractive index than the MMA/IPMI copolymer which depended on the contents of sytrene.

Detecting a deceptive attitude in non-pressure situations using K-LIWC (K-LIWC를 이용한 비압박 상황의 거짓 태도 탐지)

  • Kim, Young-il;Kim, Youngjun;Kim, Kyungil
    • Korean Journal of Cognitive Science
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    • v.27 no.2
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    • pp.247-273
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    • 2016
  • Previous studies about lying were mainly executed in pressure situations, such as interviews or crime statements, which made people stressed. This study analyzed deceptive and non-deceptive writings in non-pressure situation through K-LIWC program, in which lies are rarely disclosed and hardly damage the liar even upon disclosure, Also, we compared these results with existing studies on lying. On both writing tasks, there were fewer first-person singular pronouns in deceptive writings than in the non-deceptive writings. The variables indicating cognitive complexity were less used by deceptive writings than by non-deceptive writings in first topic, but in the second topic, more were used by deceptive writings than true writings. In particular, previous studies claim that lies contain more negative emotional words while this report shows that lies in non-pressure situations contains more positive and fewer negative emotional words compared to truth. This finding implies that a situation influences the liar's psychological statement, which changes the contents of the lie.

Smart Phone Copyright Violation and Forensic Apply Method (Smart Phone 저작권 위반과 포렌식 적용 방안)

  • Yi, Jeong-Hoon;Park, Dea-Woo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.11
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    • pp.2491-2496
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    • 2010
  • Smart Phone with domestic demand increasing rapidly, the utilization of multimedia services have become diverse. Smart Phone users use the copyrighted multimedia contents illegally from hacking their Smart Phone with Jail Breaking and Rooting. Legal issues according to the Korea-U.S. FTA. and high relevance with crime as mobile communication terminal, the utilization of created and saved digital evidence is high, the mobile forensic evidence study is required. This paper studied method and notice of legal seizure and search assuming the Smart Phone copyright violation. Research the status of Smart Phone copyright violation and related violation by category as broadcasting, movies, music, e-book etc. Research the method of submit a report to the court by applying techniques to forensic. The results of this research will contribute to the provide of Smart Phone crime evidence and mobile forensic technology.

International Standards Activities for ISO/TC 112 Vacuum Technology (ISO/TC 112 Vacuum Technology 국제표준 활동)

  • Hong, S.S.;Shin, Y.H.;Kim, J.T.;Chung, K.H.;Choi, H.K.;Kim, I.S.;Park, W.Y.
    • Journal of the Korean Vacuum Society
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    • v.16 no.6
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    • pp.397-404
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    • 2007
  • International Standardization for Vacuum pumps, vacuum instruments, and vacuum components has been established at ISO/TC 112 which is a technical committee of ISO (International Organization for Standardization) in the area of vacuum technology. This report shortly summarizes the structure of ISO/TC 112 and its activities on the standardization for vacuum technology. Also it introduces the brief contents of "specifications for hot cathode ionization gauges" and"Test procedure for vacuum valves" which are recently accepted as new proposals. These information on ISO/TC 112 would contribute to activate the development of vacuum technology as well as the participation for the international standardization in Korea.

Brassinosteroid substances in immature Cassia tora seeds (결명자의 brassinosteroid 활성물질)

  • Park, Keun-Hyung;Kim, Seon-Jae;Hyun, Kyu-Hawn
    • Applied Biological Chemistry
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    • v.36 no.2
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    • pp.99-104
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    • 1993
  • In order to explore the brassinosteroid-active component in Cassia tora, methanol extract of immature seeds was purified by sequences of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography and Bondesil chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography. Brassinosteroid substances in separated active fractions were identified as teasterone, castasterone, brassinolide by TLC and HPLC. Our work is probably the first report of endogenous brassinosteroid in Cassia tora. The content of brassinosteroid in Cassia tora as converted into brassinolide was $3.5{\sim}5.5\;ng/g$ fresh weight. The order of brassinosteroid contents was toward to be teasterone, castasterone, brassinolide.

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Development of Introductory Engineering Design Course to Improve Creative Thinking Ability (창의적 사고능력 증진을 위한 공학설계입문 교과목 및 사례 개발)

  • Kim Lee-Hyung;Lee Byung-Sik
    • Journal of Engineering Education Research
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    • v.8 no.3
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    • pp.26-35
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    • 2005
  • Currently social and economical situations of the world including Korea are tremendously changing because of developments of various technologies including information technology. It is well known that the current world is society of globalization, knowledge and information. In order to accomodate the engineering education of Korea to the world standards, many researches about engineering education should be studied. Therefore, the purpose of this research is to develope the course of 'Introduction to Engineering Design' for undergraduate students in engineering schools. The graduates from the engineering schools should have various communication skills such as presentation, report preparation, language skills, etc. Also the graduates have to change their mind from passivity to creativity for their works. The other important things for graduates are improvement of the teamwork skills in the team and improvement of project management skills. Therefore, this paper will present the processes of the course development and the contents of the course concerning teamwork, inventive thinking, project management, and presentation skills.

Simple Rumenotomy for Removing Foreign Bodies in Himalayan Tahrs (Hemitragus jemlahicus)

  • Yong, Hwan-Yul;Lim, Yang-Mook;Cho, Dong-Gil;Bae, Bok-Soo;Kim, Seung-Dong;Kim, Sung-Ki;Hyun, Sang-Hwan
    • Journal of Embryo Transfer
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    • v.26 no.2
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    • pp.141-145
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    • 2011
  • Seven male Himalayan tahrs were strongly assumed to have accidently fed on foreign bodies in 2007. At the time, zoo caretakers witnessed missing fence padding, such as carpet, plastic awning, and ropes. The incident occurred the morning after the items had been set up to protect indigenous, wild long-tailed gorals from self injury caused by head butting the steel fence. Adult male Himalayan tahrs were obviously suspected of mostly eating the paddings mainly composed of carpet, thin and long plastic awning, and ropes. Even though they had not shown digestive problems, surgery was determined necessary in order to remove any indigestible foreign bodies. Left flank rumenotomy was conducted on seven male Himalayan tahrs from April to May, 2011. After anesthesia with xylazine, rumenotomy was performed on a concrete floor, with legs and head secured by ropes. No access to water and hay prior to operation for two days was needed to make the surgical procedure done quickly. Two sheets of small hand towels protecting against inflow of ruminal contents were beneficial during surgery. Antibiotics were administered intramuscularly for seven days. No abscesses at the surgical site were found after surgery. Like domestic ruminants, wild ruminants also ingest metalic or non-metalic, indigestible foreign bodies by accident. Therefore, simplified rumenotomy must be developed to apply to those wild animals to lengthen their longevity and to advance the quality of life in captivity. This case report is the first showing how to perform rumenotomy of Himalayan tahr, a wild ruminant, in Korea.

Adolescents' Types of Sexual Attitude and Their Perception of Sexual Abuse Incidents (청소년의 성태도와 성폭력 사건 인식에 대한 연구)

  • Yi, Yeong-Sug
    • Korean Journal of Human Ecology
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    • v.14 no.5
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    • pp.709-720
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    • 2005
  • The purpose of this study is to provide practical information which can prevent the adolescents from sexual derailment by identifying types of sexual attitudes and their perception of sexual abuse incidents. The results are as followings; 1. The sexual attitudes were grouped into three types: passive type, active chaser, and conservative introvert. Most adolescents belonged to passive type and least to trend of chaser. The results showed that Korean adolescents tended to be passive to the matter of sex. They also had different perceptions to sexual attitude and sexual abuse incidents in relation to their sexual attitude type, sex and grade. Thus, sex education with different contents and approaches in relation to their sexual attitude type, gender and age seems to be effective. 2. Majority of the adolescents thought sexual abuses could take place at anytime and anywhere. Belief that the offender would be a male and the victim would be a female was most common. Most respondents thought the offender would be responsible for sexual abuse, and that the offender would be a male. Most were the replies that one would report the offender and go to counselling center for help. Although these replies are very desirable, there are doubts that if they would be able to behave that way in the actual situation. That is because it is hard to get help from counselling center due to the system and the lack of counselors. Therefore, it is essential to improve the system to make it possible to give practical help to adolescents.

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The Practical Application Strategy of Video Production for Recording an Archeology (고고학 기록을 위한 영상제작 활용 방안)

  • Park Soon-Hong;Jeon Byeong-Ho
    • Journal of Game and Entertainment
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    • v.2 no.1
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    • pp.45-55
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    • 2006
  • The video production, as a recording data about the archeological survey, has advantages to be visualized in actuality and historical fact presented objects with elements such as moving images, audio(voice) and text(subtitle). While the report with text and images such as photographs and drawings does not present exactly, the video production can record a concrete excavation process to be difficult of represent with former media. To make a practical use the video production in archeological surveys, the use dimension should be expanded to the entire archeological excavations and the shooting and editing work should be done by those video production-specialized staffs. Ultimately, when this video production device is used but also for the survey purposes but also for the education, exhibition, and even commercial purposes, it can have the substantial meaning as a survey tool.

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