• 한국문화재보존과학회:학술대회논문집
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• 2004.10a
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• pp.75-80
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• 2004

We proposed that the replicas be made as an alternative to achieve such reciprocal goals as the safe preservation of traditional costume relics and socio-educational realizations through exhibitions, etc., A replication was categorized for its purpose into a restoral replication a work based on the historical research of color and shapes as they were originally made and a current state replication . a production based on a minute record of the relics as they are excavated Then, we reported the reproduction process from the excavation to the exhibition on the excavated traditional costumes of Milchanggun's Jobok The purpose of a replication of relics is to record the relics experiencing the change resulted from the inevitable degeneration over time as organic cultural assets together with the substitution exhibition of relics and academic researches and so on. Accordingly, the above two methods are to be preceded by a deep and through research and study on the relics of replication. This study on the relics having an important cultural property value presents the preservation of tile cultural assets of traditional costume through the two replication processes and results and a flew pattern of exhibition.

• Journal of Information Processing Systems
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• v.10 no.4
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• pp.628-645
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• 2014

In past few years, distributed hash table (DHT)-based P2P systems have been proven to be a promising way to manage decentralized index information and provide efficient lookup services. However, the skewness of users' preferences regarding keywords contained in a multi-keyword query causes a query load imbalance that combines both routing and response load. This imbalance means long file retrieval latency that negatively influences the overall system performance. Although index replication has a great potential for alleviating this problem, existing schemes did not explicitly address it or incurred high cost. To overcome this issue, we propose, in this paper, an integrated solution that consists of three replication schemes to alleviate query load imbalance while minimizing the cost. The first scheme is an active index replication that is used in order to decrease routing load in the system and to distribute response load of an index among peers that store replicas of the index. The second scheme is a proactive pointer replication that places location information of each index to a predetermined number of peers for reducing maintenance cost between the index and its replicas. The third scheme is a passive index replication that guarantees the maximum query load of peers. The result of simulations indicates that the proposed schemes can help alleviate the query load imbalance of peers. Moreover, it was found by comparison that our schemes are more cost-effective on placing replicas than PCache and EAD.

• New & Renewable Energy
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• v.19 no.4
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• pp.46-52
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• 2023

Methane is the second most emitted greenhouse gas after carbon dioxide. Despite lower emissions than those of carbon dioxide, methane receives significant attention owing to its more than 20-fold higher global warming potential. Consequently, the importance of research on methanotrophic bacteria, microorganisms capable of converting methane gas into high-value materials, is increasingly emphasized. In the case of methanotrophic bacteria, knowledge on episomal plasmids that can be used for genetic engineering remains lacking, which poses significant challenges to the engineering process. The replication origin sequences of natural plasmids within methanotrophic bacteria have been predicted through in silico methods. The basic characteristics of the replication origin, such as a high A/T ratio, repetitive sequences, and proximity to proteins related to replication, have been used as criteria for identifying the replication origin. As a result, a region with a sequence of 18 base pairs repeated eight times could be identified. The putative replication origin sequence thus identified generally takes the form of iterons, but it also possesses unique features such as the length of the gap between iterons and the repetition of identical iteron sequences. This information can be valuable for future design of episomal plasmids applicable to methanotrophs.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1412-1419
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• 2020

Human noroviruses (HuNoVs) are a leading cause of gastroenteritis outbreaks worldwide. However, the paucity of appropriate cell culture models for HuNoV replication has prevented developing effective anti-HuNoV therapies. In this study, first, the replication of the virus at various temperatures in different cells was compared, which showed that lowering the culture temperature from 37℃ significantly increased virus replication in Madin-Darby canine kidney (MDCK) cells. Second, the expression levels of autophagy-, immune-, and apoptosis-related genes at 30℃ and 37℃ were compared to explore factors affecting HuNoV replication. HuNoV cultured at 37℃ showed significantly increased autophagy-related genes (ATG5 and ATG7) and immune-related genes (IFNA, IFNB, ISG15, and NFKB) compared to mock. However, the virus cultured at 30℃ showed significantly decreased expression of autophagy-related genes (ATG5 and ATG7), but not significantly different major immune-related genes (IFNA, ISG15, and NFKB) compared to mock. Importantly, expression of the transcription factor FOXO1, which controls autophagy- and immune-related gene expression, was significantly lower at 30℃. Moreover, FOXO1 inhibition in temperature-optimized MDCK cells enhanced HuNoV replication, highlighting FOXO1 inhibition as an approach for successful virus replication. In the temperature-optimized cells, various HuNoV genotypes were successfully replicated, with GI.8 showing the highest replication levels followed by GII.1, GII.3, and GII.4. Furthermore, ultrastructural analysis of the infected cells revealed functional HuNoV replication at low temperature, with increased cellular apoptosis and decreased autophagic vacuoles. In conclusion, temperature-optimized MDCK cells can be used as a convenient culture model for HuNoV replication by inhibiting FOXO1 and providing adaptability to different genotypes.

• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1163-1178
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• 2010

Plasmids play important roles in horizontal gene transfer among Vibrionaceae, but surprisingly little is known about their replication and incompatibility systems. In this study, we successfully developed a bioinformatics-assisted strategy of experimental identification of seven Vibrio plasmid replicons. Comparative sequences analysis of the seven Vibrio plasmid replicons obtained in this study together with eight published Vibrionaceae plasmid sequences revealed replication-participating elements involved in the ColE1 mode of replication initiation and regulation. Like plasmid ColE1, these Vibrionaceae plasmids encode two RNA species (the primer RNA and the antisense RNA) for replication initiation and regulation, and as a result, the 15 Vibrionaceae plasmids were designated as ColE1-like Vibrionaceae (CLV) plasmids. Two subgroups were obtained for the 15 CLV plasmids, based on comparison of replicon organization and phylogenetic analysis of replication regions. Coexistence of CLV plasmids were demonstrated by direct sequencing analysis and Southern hybridization, strongly suggesting that the incompatibility of CLV plasmids is determined mainly by the RNA I species like the ColE1-like plasmids. Sequences resembling the conserved Xer recombination sites were also identified on the CLV plasmids, indicating that the CLV plasmids probably use the host site-specific recombination system for multimer resolution like that used by ColE1-like plasmids. All the results indicated that the 15 plasmids form a new ColE1-like group, providing a basis for the rapid characterization and classification of Vibrionaceae plasmids.

• Molecules and Cells
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• v.44 no.2
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• pp.101-115
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• 2021

The INO80 chromatin remodeling complex has roles in many essential cellular processes, including DNA replication. However, the mechanisms that regulate INO80 in these processes remain largely unknown. We previously reported that the stability of Ino80, the catalytic ATPase subunit of INO80, is regulated by the ubiquitin proteasome system and that BRCA1-associated protein-1 (BAP1), a nuclear deubiquitinase with tumor suppressor activity, stabilizes Ino80 via deubiquitination and promotes replication fork progression. However, the E3 ubiquitin ligase that targets Ino80 for proteasomal degradation was unknown. Here, we identified the C-terminus of Hsp70-interacting protein (CHIP), the E3 ubiquitin ligase that functions in cooperation with Hsp70, as an Ino80-interacting protein. CHIP polyubiquitinates Ino80 in a manner dependent on Hsp70. Contrary to our expectation that CHIP degrades Ino80, CHIP instead stabilizes Ino80 by extending its half-life. The data suggest that CHIP stabilizes Ino80 by inhibiting degradative ubiquitination. We also show that CHIP works together with BAP1 to enhance the stabilization of Ino80, leading to its chromatin binding. Interestingly, both depletion and overexpression of CHIP compromise replication fork progression with little effect on fork stalling, as similarly observed for BAP1 and Ino80, indicating that an optimal cellular level of Ino80 is important for replication fork speed but not for replication stress suppression. This work therefore idenitifes CHIP as an E3 ubiquitin ligase that stabilizes Ino80 via nondegradative ubiquitination and suggests that CHIP and BAP1 act in concert to regulate Ino80 ubiquitination to fine-tune its stability for efficient DNA replication.

• Journal of the Korea Society of Computer and Information
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• v.14 no.5
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• pp.37-44
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• 2009

Recently, as the quality of multimedia data gets higher, multimedia servers require larger storage capacity and higher I/O bandwidth. In these large scale multimedia servers, the load-unbalance problem among disks due to the difference in access frequencies to multimedia objects according to their popularities significantly affects the system performance. To address this problem, many data replication schemes have been proposed. In this paper, we propose a novel data migration/replication scheme to provide better storage efficiency and performance than the dynamic data replication scheme which is typical data replication scheme employed in multimedia servers. This scheme can reduce the additional storage space required for replication, which is a major defect of replication schemes, by decreasing the number of copies per object. The scheme can also increase the number of concurrent users by increasing the caching effect due to the reduced lengths of the intervals among requests for each object.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2013.05a
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• pp.605-606
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• 2013

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.05a
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• pp.205-208
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• 2007

We injection molded a thin plate with micro prism patterns on its surface and investigated the fidelity of replication of the micro pattern depending on the process parameter such as mold temperature, injection rate or packing pressure. The size of the $90^{\circ}$ prism pattern is $50{\mu}m$ and the size of the plate is $400mm{\times}400mm$. The thickness is 1mm. The fidelity of the replication turned out quite different according to the process parameters and location of the patterns of the plate. We measured the cavity pressure and temperature in real-time during the molding to analyze the effect of the local melt pressure and temperature on the micro pattern replication.

• Molecules and Cells
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• v.28 no.3
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• pp.149-154
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• 2009

Faithful and accurate replication of the DNA molecule is essential for eukaryote organisms. Nonetheless, in the last few years it has become evident that inheritance of the chromatin states associated with different regions of the genome is as important as the faithful inheritance of the DNA sequence itself. Such chromatin states are determined by a multitude of factors that act to modify not only the DNA molecule, but also the histone proteins associated with it. For instance, histones can be posttranslationally modified, and it is well established that these posttranslational marks are involved in several essential nuclear processes such as transcription and DNA repair. However, recent evidence indicates that posttranslational modifications of histones might be relevant during DNA replication. Hence, the aim of this review is to describe the most recent publications related to the role of histone posttranslational modifications during DNA replication.