• Biotechnology and Bioprocess Engineering:BBE
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• 제7권2호
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• pp.67-75
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• 2002

The human complement receptor type 1 (CR 1, C3 b/C4b receptor) is a polymorphic membrane glycoprotein expressed on human erythrocytes, peripheral leukocytes, plasma and renal glomerular podocytes, which consists of transmembrane and cytoplasmic domains with 30 repeating homologous protein domains known as short consensus repeats (SCR). CR1 has been used as an inhibitor for inflammatory and immune system for the past several years. Recently; it is reported that CRl was found to suppress the hyper-acute rejection in xeno-transplantation and can be used to cure autoimmune diseases. A soluble form of CRl, called sCRl, is a recombinant CRl by cleaving the transmembrane domain at C-terminus and has been expressed in Chinese Hamster Ovary (CHO) cells. Several purification methods for sCR1 from CHO cells have been reported, but most of them require complicated steps at high cost. Moreover, such methods are mostly performed under the pH condition apt to denaturing sCR1 and causes sCRl losing its activity. We here report a rapid and efficient method to purify sCR1 from CHO cell. The new method consists of a two-stage of cell culture by cultivating cells in serum medium followed by serum-free medium, and a two-stage of column purification by means of heparin and gel filtration column chromatography. By using this novel method, sCR1 can be purified in a simple and effective way with high yield and purity, furthermore, the purified sCR1 was confirmed to retain its activity to suppress the complement activation in vivo and ex vivo.

• Journal of International Academy of Physical Therapy Research
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• 제7권2호
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• pp.989-993
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• 2016

The purpose of this study was to investigate the effects of occipital bone stimulation by cervical stabilizing exercise on the muscle tone, stiffness, ROM, and cervical lordosis in patient with forward head posture(FHP). This study was a case study of a single patient with forward head posture. This study used a ABA' design, A and A' were the baseline phases and B was the intervention phase. The intervention was occipital bone stimulation by cervical stabilization exercise. It was administered once daily for 7 days. The therapist kept hands together, and placed the two index fingers under the subject's occipital bone. The subject performed the chin-in exercise with a maximum isometric contraction for 20 sec.The exercise was implemented by performing the movements 10 times as a set and repeating the set three times. The muscle tone was not significantly changed after intervention. However, the stiffness was decreased and lasted the effect lasted without intervention. The cervical flexion angle was increased, but the cervical extension angle was not significantly changed after the intervention. The left and right lateral flexion angles were increased and the effect lasted without any intervention. However, the left and right rotation angles were significantly changed after the intervention. Cervical lordosis increased not from $37^{\circ}$ to $41^{\circ}$ after the intervention. These results suggest that occipital bone stimulation by cervical stabilizing exercise had a positive effect on cervical stiffness, flexion and lateral flexion ROM, and lordosis in a patient with forward head posture.

• Journal of the Korea Institute of Information and Communication Engineering
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• 제8권4호
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• pp.826-832
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• 2004

A CMOS 8 bit folding and interpolating ADC for an embedded system inside VLSI is presented in this paper. This folding ADC uses the 2 stage architecture for improving of nonlinearity. repeating the folding and interpolating twice. At a proposed structure, a transistor differential pair operates on the second folder. A ADC with 2 stage architecture reduces the number of comparators and resisters. So it is possible to provide small chip size, low power consumption and high operating speed. The design technology is based on fully standard 0.25m double-Poly 2 metal n-well CMOS Process. The simulated Power consumption is 45mW with an applied voltage of 2.5V and sampling frequency of 250MHz. The INL and DNL are within <ㅆㄸㅌ>$\pm$0.2LSB, respectively. The SNDR is approximately 45dB for input frequency of 10MHz.

• Journal of Korean Society of Industrial and Systems Engineering
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• 제38권4호
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• pp.226-232
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• 2015

The aging of Korea is rapidly advancing in an unprecedented rate in the world. Indoor and outdoor safety accidents involving the elderly frequently occur, with elderly population increasing. Because the physical strength of elderly is decreased, it is necessary to develop devices that aid the everyday life of the elderly to prevent safety accidents. Among many activities, the motions of standing from and sitting on a chair require much physical strength from particular body parts, which causes an overload for particular body parts. To the elderly the repeating motion of standing from and sitting down on a chair is burdensome, because they do not have enough physical strength compare to young persons. As a result the elderly could be injured or falling during standing or sitting. Therefore, the development of a lift chair that considers the problems mentioned above is necessary to prevent the safety accidents of the elderly. The study designed a non-motorized lift chair that comfortably aids the standing and sitting down motion for the use of the elderly by reflecting the anthropometric data of the Korean elderly over 65 years old. Also the design was implemented to smoothly aid the standing motion by considering the knee angle of the elderly when standing. Because the regained strength to stand from a chair is depend on the knee angle. Unlike existing lift devices, this device utilizes a tilting mechanism that does not use electric power so that the product could be composed inexpensively than existing chairs released in the market.

• The Journal of the Korea Contents Association
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• 제21권2호
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• pp.587-593
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• 2021

In this thesis, we will break away from the classic DDoS response system for large-scale denial-of-service attacks that develop day by day, and effectively endure intelligent denial-of-service attacks by utilizing artificial intelligence-based technology, one of the core technologies of the 4th revolution. A possible service model development plan was proposed. That is, a method to detect denial of service attacks and minimize damage through machine learning artificial intelligence learning targeting a large amount of data collected from multiple security devices and web servers was proposed. In particular, the development of a model for using artificial intelligence technology is to detect a Western service attack by focusing on the fact that when a service denial attack occurs while repeating a certain traffic change and transmitting data in a stable flow, a different pattern of data flow is shown. Artificial intelligence technology was used. When a denial of service attack occurs, a deviation between the probability-based actual traffic and the predicted value occurs, so it is possible to respond by judging as aggressiveness data. In this paper, a service denial attack detection model was explained by analyzing data based on logs generated from security equipment or servers.

• Composites Research
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• 제35권1호
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• pp.8-12
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• 2022

Analysis of defect signals inside glass fiber reinforced polymer (GFRP) was conducted through deconvolution of terahertz (THz) wave. The GFRP specimen with internal defects was manufactured and the THz signal was measured through the reflection mode of the Terahertz Time-Domain Spectroscopy (THz-TDS) system. For deconvolution of the measured THz signal, the peak position of the THz signal was amplified through Normalized Cross Correlation (NCC) of the incident and detected THz signals. The position and intensity of the amplified peak were extracted as impulse, and the extracted signal of the impulse position was removed from the THz original signal. By repeating the process, the critical impulses, which represent boundary of the specimen, were derived. The deconvolution process was verified by confirming that the original THz signal without noise can be restored through the convolution of the critical impulses and the incident signal. From the derived critical impulses, the thickness of the internal defect in the GFRP was calculated through the detection time of impulses within 15 ㎛ accuracy.

• Journal of the Korea Society of Computer and Information
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• 제14권1호
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• pp.99-106
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• 2009

Though edge detection, an important stage that significantly affecting the performance of image recognition, has been given numerous researches on its execution methods, it still remains as difficult problem and it is one of the components for image recognition applications while it is not the only way to identify an object or track a specific area. This paper, unlike gradient operator using edge detection method, found out edge pixel by referring to 2 neighboring pixels information in binary image and comparing them with pre-defined 4 edge pixels pattern, and detected binary image edge by determining the direction of the next edge detection exploring pixel and proposed method to detect binary image edge by repeating step of edge detection to detect another area edge. When recognizing image, if edge is detected with the use of gradient operator, thinning process, the stage next to edge detection, can be omitted, and with the edge detection algorithm executing time reduced compared with existing area edge tracing method, the entire image recognizing time can be reduced by applying real-time image recognizing system.

• Korean Journal of Agricultural Science
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• 제48권4호
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• pp.1027-1037
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• 2021

This study aims to evaluate the performance of an electric multi-purpose cultivator through a simulation analysis. The simulation model was developed using commercial software, Simulation X, by applying the specifications of certain parts, such as an electric motor, a battery, and so on. The input parameter of the simulation was the engine load data according to the rotary tillage level using a conventional multi-purpose cultivator. The data were collected by configuring a load measurement system, and the load cycle was developed by repeating the data collection process under the most severe conditions. The average output engine torque values of conventional multi-purpose cultivator were 10.7, 13.0, 9.4, and 11.2 Nm in the D1P1, D1P2, D2P1, and D2P2 conditions, respectively. As a result of the simulation, the maximum values of the motor torque, rotational speed, and power of the electric multi-purpose cultivator were 16.8 Nm, 2,033.3 rpm, and 3.3 kW, respectively, and the motor was driven in sections within 70, 68, and 45% of the maximum output range. The rate of decrease of the battery state of charge (SOC) level per minute was approximately 0.6%, and it was possible to supply electric power to the motor for 9,550 sec. In the future study, research to verify and improve simulation models of electric multi-purpose cultivators should be conducted.

• Journal of Advanced Navigation Technology
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• 제27권5호
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• pp.502-509
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• 2023

This study presents a satellite constellation method that achieves optimal revisit performance by utilizing genetic algorithm techniques. The Walker method is a global coverage concept, and there are limitations to target-centered constellation considering the strategic environment of the Korean Peninsula. To overcome these limitations, targets are set in major areas of interest in North Korea, orbit elements with optimal revisit performance for each target are searched, and based on this, the number of satellites optimized for each target is derived using a genetic algorithm. The results of this study demonstrate the performance of the optimized constellation by applying phasing rules to achieve the desired revisit performance.

• Journal of the Institute of Electronics and Information Engineers
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• 제49권10호
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• pp.194-201
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• 2012

Moxibustion is utilized not only to cure disease but also to increase immunity. However, it may lead to undesired effects including severe pains(blisters and burns) because of the difficulty of controlling heat intensity. To overcome these problems we developed the RF heat stimulation system which can control stimulus. Also, we developed the RF stimulation protocol for effective heat transfer in subcutaneous tissue of rabbit. RF stimulator consists of a medical RF capacitive heating device, isolation probe, isolation plate, negative pressure control part and temperature measurement part. For the designed stimulus protocol, we measured the temperature distribution on epidermis and in subcutaneous(5mm, 10mm) area of rabbit during moxibustion. A stimulation protocol was designed by controlling the ON/OFF duty ratio, repeating number, and energy of applied pulse to get the temperature distribution similar with that by moxibustion. In results, the correlation coefficients between temperature distribution by moxibustion and that of stimulator were 95% and 91% from 5mm and 10mm thick respectively. However, temperature distribution on epidermis by stimulator was remarkably lower than that of the moxibustion. Finally, the RF stimulation system showed that it can not only transfer effectively heat similar with moxibustion to the subcutaneous area, but also the influence by unwanted side effects can be minimized.