• Proceedings of the Korea Concrete Institute Conference
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• 1997.04a
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• pp.579-584
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• 1997

This experimentation is to apperciation an efficiency of repair for processing crack by corrosion of steel reinforcing, Crack on concrete by corrosion of steel reinforcing is the fact that the first crack appear on the surface of water because of supplying is of oxygen and water. The crack processing is on surface to be contacted by air and to bottom as mainly the vertical direction from a surface of water. The experimentation gives rise to crack in model by electricity. Crack by corrosion of steel reinforcing is more internal crack than external crack. since it is so. crack by corrosion of steel reinforcing have to attention to repair or intermal crack.

• The Journal of Dong Guk Oriental Medicine
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• v.2 no.1
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• pp.127-137
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• 1993

In order to investigate the clinical efficiency based on Oriental Medical References of Hyang-Sa-Yuk-Gun-Ja-Tang and Oh-Pe-San, histological studies have been done by using the experimental animal. The following results were obtained. 1. The Hyang-Sa-Yuk-Gun-Ja-Tang showed a significant promoting effect of repair on the epithelial layer from the basement membrane of duodenal ulcer in rats. 2. The Oh-Pe-San showed efficiencies about repair of the necrosis in epithelial layer on the acute gastritis in rats. According to above results, it is concluded that Oh-Pe-San and Hyang-Sa-Yuk-Gun-Ja-Tang were recognized to be effective on the acute gastritis and the duodenal ulcer in rats, and this study may suggest a clinical guidance to the prescriptions for the treatment of gastritis end gastric ulcer.

• Journal of the Korean Society for Railway
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• v.15 no.6
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• pp.638-644
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• 2012

In this study, we numerically estimated available and unavailable time of conventional railway by examining 60% which is known as average range of track utilization efficiency. We also estimated track repair time for maintenance which is the main factor of making track utilization change and analyzed appropriate estimated time. The railway's safety should be guaranteed by appropriate railway operating plan and by adequate rail facilities maintenance. At the same time, daily railway train 'Dia's recovery power should be reflected to the management plan. Considering these factors, we examined mechanical equipment and the national rail network in order to rationally estimate track repair time of the maintenance and to secure spare time in case of train delays. It was found that track utilization efficiency was more or less 60%.

• BMB Reports
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• v.56 no.2
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• pp.102-107
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• 2023

Genome editing using CRISPR-associated technology is widely used to modify the genomes rapidly and efficiently on specific DNA double-strand breaks (DSBs) induced by Cas9 endonuclease. However, despite swift advance in Cas9 engineering, structural basis of Cas9-recognition and cleavage complex remains unclear. Proper assembly of this complex correlates to effective Cas9 activity, leading to high efficacy of genome editing events. Here, we develop a CRISPR/Cas9-RAD51 plasmid constitutively expressing RAD51, which can bind to single-stranded DNA for DSB repair. We show that the efficiency of CRISPR-mediated genome editing can be significantly improved by expressing RAD51, responsible for DSB repair via homologous recombination (HR), in both gene knock-out and knock-in processes. In cells with CRISPR/Cas9-RAD51 plasmid, expression of the target genes (cohesin SMC3 and GAPDH) was reduced by more than 1.9-fold compared to the CRISPR/Cas9 plasmid for knock-out of genes. Furthermore, CRISPR/Cas9-RAD51 enhanced the knock-in efficiency of DsRed donor DNA. Thus, the CRISPR/Cas9-RAD51 system is useful for applications requiring precise and efficient genome edits not accessible to HR-deficient cell genome editing and for developing CRISPR/Cas9-mediated knockout technology.

• Molecules and Cells
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• v.38 no.1
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• pp.33-39
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• 2015

The correction of disease-causing mutations by single-strand oligonucleotide-templated DNA repair (ssOR) is an attractive approach to gene therapy, but major improvements in ssOR efficiency and consistency are needed. The mechanism of ssOR is poorly understood but may involve annealing of oligonucleotides to transiently exposed single-stranded regions in the target duplex. In bacteria and yeast it has been shown that ssOR is promoted by expression of $Red{\beta}$, a single-strand DNA annealing protein from bacteriophage lambda. Here we show that $Red{\beta}$ expression is well tolerated in a human cell line where it consistently promotes ssOR. By use of short interfering RNA, we also show that ssOR is stimulated by the transient depletion of the endogenous DNA mismatch repair protein MSH2. Furthermore, we find that the effects of $Red{\beta}$ expression and MSH2 depletion on ssOR can be combined with a degree of cooperativity. These results suggest that oligonucleotide annealing and mismatch recognition are distinct but interdependent events in ssOR that can be usefully modulated in gene correction strategies.

• Journal of Radiation Protection and Research
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• v.20 no.2
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• pp.71-78
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• 1995

To develop methods to reduce radiation risk and apply such knowledge to improvement of radiation protection, the effects of cobaltous chloride known as bioantimutagen on the function of E. coli RecA protein involved in the repair of DNA damage were examined. The results demonstrated two distinct effects of cobaltous chloride on the RecA protein function necessary for the strand exchange reaction. Cobaltous chloride enhanced the ability of RecA protein to displace SSB protein from single-stranded DNA and the duplex DNA-dependent ATPase activity. RecA protein was preferentially bound with UV-irradiated supercoiled DNA as compared with nonirradiated DNA The binding of RecA protein to UV-irradiated supercoiled DNA was enhanced in a dose-dependent manner. It is likely that studies on the factors affecting repair efficiency and the DNA repair proteins may provide information on the repair of ionizing radiation-induced DNA damage and the mechanism for DNA radioprotection.

• Corrosion Science and Technology
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• v.20 no.5
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• pp.308-314
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• 2021

Neutron dose level at bottom head of a reactor pressure vessel (RPV) was calculated using reactor vessel neutron transport for a Korean nuclear power plant A. At 34 EFPY with a 40-year (2042) design life after plating repair, irradiation fast neutron effect was 6.6x10¹⁵ n/cm². As helium(He) gas can be generated by Ni only at 1/10⁶ level of 5 × 10²¹ n/cm², He generation possibility in the Ni plating layer is very little during 40 years of operation (2042, 34 EFPY). Thermal neutrons can significantly affect the generation of He from Ni metal. At 10 years after a repair, He can be generated at a level of about 0.06 appm, a level that can add general welding repair without any consideration. After 40 years of repair, 9.8 appm of He may be generated. Although this is a rather high value, it is within the range of 0.1 to 10 appm when welding repair can be applied. Clad repair by Ni electroplating technology is expected to greatly improve the operation efficiency by improving the safety and shortening the maintenance period of the nuclear power plant.

• Korean Journal of Environmental Biology
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• v.29 no.3
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• pp.236-240
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• 2011

Mercury known as quicksilver, is the most common cause of heavy metal toxicity. Toxicity caused by excessive mercury exposure is now being recognized as a widespread environmental problem and is continuing to attract a great deal of public concerns. The mercury genotoxicity could be its effect on DNA repair mechanisms, which constitute the defense system designated to protect genome integrity. The objective of this study is to confirm that mercuric chloride inhibits the repair of gamma ray-induced DNA damage. The earthworm of Eisenia fetida was chosen for this study because it is an internationally accepted model species for toxicity testing with a cosmopolitan distribution. Experiments were done to identify the levels of DNA damage and the repair kinetics in the coelomocytes of E. fetida irradiated with 20 Gy gamma rays alone or with gamma rays after 40 mg $kg^{-1}$ $HgCl_2$ treatment by means of the single cell gel electrophoresis assay. The Olive tail moments were measured during 0~96 hours after irradiation. The repair time in the animals treated with the combination of $HgCl_2$ and ionizing radiation was nearly five times longer than that in the animals treated with ionizing radiation alone. Also, E. fetida exposed to mercury showed a statistically lower repair efficiency of gamma ray-induced DNA damage. The results suggest that the mercury could even have deleterious effects on the DNA repair system. Influence of mercury on the DNA repair mechanisms has been confirmed by this study.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.941-944
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• 2008

Various ductile fiber reinforced cement composite(DFRCC) including large quantities of PVA fiber or steel fiber have been developed recently and studies to find applications in diverse domains are currently conducted actively. Regard to economical efficiency, DFRCC becomes competitive when applied as special elements and repair material with small quantities rather than the casting of large volume for the main body of structures in field. The authors have developed FRP-DFRCC composite slab for bridges and a wet spraying repair technique using DFRCC. In case of the application on FRP-DFRCC composite slab, it was found that there was no problems the structure and durability of it after passed 3 months. And in case of the application on the application of the deteriorated sewage box that passed 20 years, it was found that there was no difference the repair performance between domestic PVA fiber and the Japan. Therefore, DFRCC using PVA fiber, the concrete structures can be increased to performance and secured the economical efficiency.

• ETRI Journal
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• v.42 no.1
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• pp.118-128
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• 2020

A semitransparent photovoltaic-thermal (PV/T) air collector can produce electricity and heat simultaneously. To maximize the thermal and overall efficiency of the semitransparent PV/T air collector, its availability should be maximum; this can be determined through a Markov analysis. In this paper, a Markov model is developed to select an optimized number of semitransparent PV modules in service with five states and two states by considering two parameters, namely failure rate (λ) and repair rate (μ). Three artificial neural network (ANN) models are developed to obtain the minimum cost, minimum temperature, and maximum thermal efficiency of the semitransparent PV/T air collector by setting its type appropriately and optimizing the number of photovoltaic modules and cost. An attempt is also made to achieve maximum thermal and overall efficiency for the semitransparent PV/T air collector by using ANN after obtaining its minimum temperature and available solar radiation.