• Journal of Gastric Cancer
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• v.10 no.3
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• pp.91-98
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• 2010

Purpose: Silent mating-type information regulation 2 homologue 1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent deacetylase. SIRT1 plays an important role in the regulation of cell death/survival and stress response in mammals. The aim of this study was to investigate whether the SIRT1 gene is involved in the development or progression of gastric cancers. Materials and Methods: SIRT1 and p53 genes in 86 gastric cancers were examined for genetic alterations by PCR-single strand conformation polymorphism sequencing, as well as SIRT1 protein expression in 170 gastric cancers by immunohistochemistry. Results: In the genetic analysis, we found SIRT1 and p53 mutations in two and 12 cases, respectively. Two missense mutations, c.599 C>T (T200I) and c.1258 G>A (E420K), were detected in the SIRT1 gene coding region. The SIRT1 and p53 mutation were found in mutually exclusive gastric cancers. The immunohistochemistry revealed that SIRT1 overexpression was found in 95 (55.9%) of 170 gastric cancers. Altered SIRT1 expression was not statistically associated with clinicopathological parameters, including tumor differentiation, location, lymph node metastasis, or p53 expression. Two cases with an SIRT1 mutation showed increased SIRT1 expression. Conclusions: These results suggest that genetic alterations and overexpression of the SIRT1 gene may contribute to gastric cancer development.

• Journal of the Korean Institute of Rural Architecture
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• v.10 no.2
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• pp.61-70
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• 2008

The purpose of this study is to give some suggestions for the legal supplement of the regulation on the balcony expansion for the optimal use of apartment balcony. For the purpose, this study focused on the characteristics of spaces expanded to the balcony, in the plans supplied after the legalization of balcony expansion, comparing them by their location; the metropolitan area of Seoul Gyeonggi and the districts of the other areas. The 168 plans of apartments in the supplied by top 10 constructors in the metropolitan areas of Seoul and Gyeonggi, together with the 92 plans in the other smaller districts in 2006 to 2007 were analyzed with the tools of frequency and chi-square analysis. The result is as follows. (1) The region showed no difference in the plans of balcony; whereas the size, the position and access of the shelter explained some differences of balcony plan. (2) In wider plans, balconies were placed in all sides of plan-front, rear and side, and were remodeled to expand facing rooms. (3) The balcony expansion tended to increase the number of bays. (4) In almost cases, the living rooms were expanded to balcony. In smaller plans, the balconies were remodeled to supply supplementary kitchen space, splitting it from laundry. (5) The shelters were placed at the rear or side of plans, but the shelter facing room could be dangerous in case of fire. The balcony expansion, despite of legalization spirit to adopt diverse use of additional spaces for residents' needs, was only a tool for the space expansion. The amendment of the regulation is needed to accommodate these findings that the balconies should be identified by their function, that the front corridor-type balcony should be limited in length and magnitude, and that the shelter should be positioned with easy access.

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1397-1410
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• 2021

The present study was designed to determine the influence of all-trans retinoic acid (ATRA) on adipogenesis-related gene regulation in bovine intramuscular (IM) and subcutaneous (SC) adipose cells during differentiation. Bovine IM and SC adipocytes were isolated from three 19-mo-old, crossbred steers. Adipogenic differentiation was induced upon cultured IM and SC preadipocytes with various doses (0, 0.001, 0.01, 0.1, 1 µM) of ATRA. After 96 h of incubation, cells were harvested and used to measure the gene expression of CCAAT/Enhancer binding protein β (C/EBPβ), peroxisome proliferator-activated receptor (PPAR) γ, glucose transporter 4 (GLUT4), stearoyl CoA desaturase (SCD), and Smad transcription factor 3 (Smad3) relative to the quantity of ribosomal protein subunit 9 (RPS 9). Retinoic acid receptor (RAR) antagonist also tested to identify the effect of ATRA on PPARγ -RAR related gene expression in IM cells. The addition of ATRA to bovine IM decreased (p < 0.05) expression of PPARγ. The expression of PPARγ was also tended to be downregulated (p < 0.1) in high levels (10 µM) of ATRA treatment in SC cells. The treatment of RAR antagonist increased the expression of PPARγ in IM cells. Expression of C/EBPβ decreased (p < 0.05) in SC, but no change was observed in IM (p > 0.05). Increasing levels of ATRA may block adipogenic differentiation via transcriptional regulation of PPARγ. The efficacy of ATRA treatment in adipose cells may vary depending on the location.

• Journal of the Korean Institute of Gas
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• v.16 no.4
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• pp.1-7
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• 2012

Systematic safety research by Korea Government has been made to enhance the safety of CNG (compressed natural gas) vehicles since the burst of compressed cylinder of an urban bus in August 9, 2010. This article summarizes some major activities to ensure the safety of CNG vehicles, which covers review of regulation, safety management system including standard of inspection and certification, and training program of inspectors and car mechanics. Specifically, the followings were reviewed; type of CNG cylinder, location of CNG cylinder, material and type of fuel line and vent line, modification of pipeline connection, installation of gas detector, installation of emergency shutdown valve, installation of protecting cover for cylinder, obligations for CNG vehicle filling station. improving periodical inspection, routine test on gas vehicles, training program for engaged in gas vehicles, and designation of safety manager for CNG bus company. This paper suggests how to improve safety of CNG vehicles as a result of review of above mentioned check items.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.14 no.2
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• pp.181-190
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• 2019

The purpose of this study is to help the understanding of the housing market through the influence of consumer choice attributes and financial policy on home buyer behavior. The key issue in the analysis is to take into account moderate effect of housing investment demand between different types of housing attribute choice and financial policy. The results of the study are as follows. First, convenience, education location, and neighborhood level have a significant effect on the purchase intention of the housing. Second, government policy have no significant influence on the purchase intention of the house. Third, the moderating effects of real estate investment outlook are that the neighbors level and interaction variables have a statistically significant effect on the purchase intention of the house. Since the government's financial policies do not affect the decision to buy a house, in reality, excessive regulation may reduce the quality of housing welfare for the first time home buyers. As a result of this study, the financial policy of the government does not affect the decision of the purchase of the house. In reality, the excessive regulation may reduce the quality of the housing welfare for the first time home buyer. Only an analysis which combines these aspects of consumer's choice can adequately describe and explain the actual change in demand in the residential market.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.38 no.2
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• pp.131-140
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• 2020

Recently the importance of BIM (Building Information Modeling) that enables 3D location-based design and construction work is being highlighted around the world. In Korea, the road map has been established to settle the design based on BIM using drone survey results by 2025. As the first step, BIM would be applied to road construction projects worth more than 50 billion Korean Won from 2020. On the other hand, drone survey regulation has been enacted and the data for drone survey cost were also included on Standard of construction estimate in 2020. However, more careful improvement is required to reflect drone survey results in BIM design and construction. Currently, Engineering instructions and Standard of construction estimate specifies that earthwork volume must be calculated by cross section method only. So it is required to add the method of DEM (Digital Elevation Model) based volume calculation on these regulations to realize BIM application. In order for that, this study verified the method of DEM based earthwork volume calculation. To get an accurate DEM for accurate volume computation, drone survey was carried out according to the drone survey regulation and then could get an accurate DEM data which have errors less than 3cm in X, Y and 6.8cm in H. As each DEM cell has 3D coordinate component, the volume of each cell can be calculated by obtaining the height of area of the cell then total volume is calculated by multiplying total number of cells by volume of each cell for the construction area. Verification for the new calculation method compare with existing method was carried out. The difference between DEM based volume by drone survey and cross section based volume by traditional survey was less than 1.33% and it can be seen that new DEM method will be able to be applied to BIM design and construction instead of cross section method.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5789-5795
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• 2013

Background: The high mobility group box 1 (HMGB1) protein is a widespread nuclear protein present in most cell types. It typically locates in the nucleus and functions as a nuclear cofactor in transcription regulation. However, HMGB1 can also localize in the cytoplasm and be released into extracellular matrix, where it plays critical roles in carcinogenesis and inflammation. However, it remains elusive whether HMGB1 is relocated to cytoplasm in clear cell renal cell carcinoma (ccRCC). Methods: Nuclear and cytoplasmic proteins were extracted by different protocols from 20 ccRCC samples and corresponding adjacent renal tissues. Western blotting and immunohistochemistry were used to identify the expression of HMGB1 in ccRCC. To elucidate the potential mechanism of HMGB1 cytoplasmic translocation, HMGB1 proteins were enriched by immunoprecipitation and analyzed by mass spectrometry (MS). Results: The HMGB1 protein was overexpressed and partially localized in cytoplasm in ccRCC samples (12/20, 60%, p<0.05). Immunohistochemistry results indicated that ccRCC of high nuclear grade possess more HMGB1 relocation than those with low grade (p<0.05). Methylation of HMGB1 at lysine 112 in ccRCC was detected by MS. Bioinformatics analysis showed that post-translational modification might affect the binding ability to DNA and mediate its translocation. Conclusion: Relocation of HMGB1 to cytoplasm was confirmed in ccRCC. Methylation of HMGB1 at lysine 112 might the redistribution of this cofactor protein.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.3
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• pp.253-268
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• 2020

This study examined the effectiveness of policy measures in a growth management plan by analyzing empirically the influence of regulations and incentives in a non-urban growth management plan of Sejong City using the binomial logistic model. The parcel unit data related development location of Sejong City from 2012 to 2017 was used in the model. The analysis showed that time regulation in the growth management plan has a negative (-) impact on the spread of development, which means it is effective in slowing urban sprawl by lowering the profits of developers. The time regulation applied in Sejong City needs to be used actively in other cities in Korea to prevent urban sprawl. Nevertheless, floor ratio incentives had no influence in inducing development within the growth management area, which means a new incentive policy to meet the local characteristics is needed to strengthen the effectiveness of the growth management plan. This study is meaningful because it attempted an empirical analysis of the effects of the growth management plan at The National Territory Act, and this study could encourage further studies.

• The Journal of the Convergence on Culture Technology
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• v.8 no.5
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• pp.477-482
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• 2022

The structure of the turnout track is very complex, so it is a facility that is more difficult to maintain and requires detailed management than a general track type. The purpose of this study is to analyze the effect on the deformation of the turnout system of the ground section due to the excavation work adjacent to the serviced urban railways. In this study, based on finite element analysis for each stage of adjacent excavation, the track deformation for each major location of turnout system was analyzed in consideration of the layout of the turnout system installed on the ground section, and the safety and stability was confirmed by comparing it with the track irregularity regulation. As a result of the study, it was found that the major construction stage affecting the track deformation of the turnout system on the ground section was the final stage of excavation. In addition, although the vertical displacement which is a vertical irregularity occurred relatively large, it was analyzed that the horizontal deformation was dominant overall, because of the excavation site is located on the side of the turnout system. In addition, it was analyzed that the amount of displacement at each major location of the turnout system is different, and there is a possibility that there may be a twist irregularity due to the deviation of the track deformation for each location according to the distance from the excavation site. Therefore, it was analyzed that it is necessary to classify and manage the importance of the track deformation of the turnout system of actual operating line, including additional displacement due to adjacent excavation, based on the track irregularity that has occurred at each location where the major deformation characteristics occur.

• Journal of The Korean Society of Agricultural Engineers
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• v.59 no.4
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• pp.75-83
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• 2017

Many parts of problems in livestock industry today are associated with organic dust. Endotoxin and toxic gasses on the surface of dust and dust itself can cause aesthetic displeasure and respiratory disease. It also reduces livestock productivity by suppressing immunity of animals and carrying microbes causing animal disease. However, dust level of cattle farm was rarely reported in Korea, and regulation for cattle farm worker does not exist. In this paper, dust concentration and environmental condition were regularly monitored in a commercial Korean native cattle farm. The measurement was conducted according to location and working activities. From the measurement, distribution of dust concentration was affected by wind environment, as the result of natural ventilation. TMR mixer was a major source of dust in target cattle house. The maximum inhalable dust concentration was 637.8 times higher than exposure limit as feed dropped into the TMR mixer. It was expected that dust generation could be affected by particle size and drop height of feed. This study suggests potential risk of dust in cattle farm, and necessity for latter study. Effect of aerodynamic condition and TMR processing should be investigated for dust reduction study.