• 한국식품영양과학회지
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• 제37권12호
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• pp.1609-1614
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• 2008

국내 주요 생산지인 전남 무안, 전북 부안, 경북 안동 및 경남 창녕의 3개 지역(유어, 장마, 성산)에서 수거된 양파 6종의 휘발성향기성분을 SPME법으로 추출하여 GC/MSD 로 분석하였다. 총 51종의 화합물이 동정되었는데, 이는 함황화합물류(20종), 알데히드류(4종), 케톤류(5종), 에스테르류(11종), 방향족화합물류(4종), 함질소화합물류(2종) 및 기타화합물류(5종)로 구성되었다. 함황화합물류가 총 휘발성 향기성분 함량의 $62.6{\sim}80.3%$를 차지하였으며, 함량 면에서는 안동(4,828 ng/g), 부안(4,627 ng/g), 유어(4,149 ng/g), 장마(2,979 ng/g), 성산(2,758 ng/g) 및 무안(2,171 ng/g) 순으로 많았다(p<0.05). 항산화효과가 있는 5종의 함황화합물류 (2,4-, 2,5-dimetylthiophene, 2-vinyl-1,3-dithiane, 5-methoxy thiazole, 3,5-diethyl-1,2,4-trithiolane)는 안동, 유어, 장마, 부안, 성산 및 무안 순이었고, 항암효과가 있는 함황화합물류 5종((Z)-, (E)-methyl propenyl disulfide, (Z)-, (E)- propenyl propyl disulfide, di-2-propenyl disulfide)은 부안, 유어, 안동, 장마, 성산 및 무안 순으로 많았다.

• Mycobiology
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• 제38권1호
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• pp.17-25
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• 2010

The common split-gilled mushroom, Schizophyllum commune is found throughout the world on woody plants. This study was initiated to evaluate conditions for favorable vegetative growth and to determine molecular phylogenetic relationship in twelve different strains of S. commune. A suitable temperature for mycelial growth was obtained at $30^{\circ}C$. This mushroom grew well in acidic conditions and pH 5 was the most favorable. Hamada, glucose peptone, Hennerberg, potato dextrose agar and yeast malt extract were favorable media for growing mycelia, while Lilly and glucose tryptone were unfavorable. Dextrin was the best and lactose was the less effective carbon source. The most suitable nitrogen sources were calcium nitrate, glycine, and potassium nitrate, whereas ammonium phosphate and histidine were the least effective for the mycelial growth of S. commune. The genetic diversity of each strain was investigated in order to identify them. The internal transcribed spacer (ITS) regions of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 129 to 143 bp and 241 to 243 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, while the 5.8S sequences were identical. A phylogenetic tree of the ITS region sequences indicated that the selected strains were classified into three clusters. The reciprocal homologies of the ITS region sequences ranged from 99 to 100%. The strains were also analyzed by random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. Twelve primers efficiently amplified the genomic DNA. The number of amplified bands varied depending on the primers used or the strains tested. The average number of polymorphic bands observed per primer was 4.5. The size of polymorphic fragments was obtained in the range of 0.2 to 2.3 kb. These results indicate that the RAPD technique is well suited for detecting the genetic diversity in the S. commune strains tested.

• 한국약용작물학회지
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• 제23권6호
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• pp.489-499
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• 2015

Background : Plants belonging to 5 species of the genus Eleutherococcus are currently distributed in the Korean peninsula. The traditional medicine 'Ogapi', derived from Eleutherococcus sessiliflorus and other related species, and 'Gasiogapi', derived from Eleutherococcus senticosus, are frequently mixed up and marketed. Therefore, accurated identification of their origins in urgently required. Methods and Results : Candidate genes from nuclear ribosomal DNA (nrDNA) and chloroplast DNA (cpDNA) of Eleutherococcus plants were analyzed. Whereas the nrDNA-internal transcribed spacer (ITS) regions were useful in elucidating the phylogenetic relationships among the plants, the cpDNA regions were not as effective. Therefore, a combined analysis with nrDNA-ITS was performed. Various combinations of nrDNA and matK were effective for discriminating among the plants. However, the matK and rpoC1 combination was ineffective for discriminating among some species. Based on these results, it was found that OG1, OG4, OG5, OG7, GS1, GS2, and GS3 were derived from E. sessiliflorus. In particular, it was confirmed that GS1, GS2, and GS3 were not derived from E. senticosus. However, more samples need to be analyzed because identification of the origins of OG2, OG3, OG6 and GS4 was not possible. Conclusion : The ITS2, ITS5a, and matK combination was the most effective in identifying the phylogenetic relationship among Eleutherococcus plants and traditional medicines based on Eleutherococcus.

• 한국지능시스템학회논문지
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• 제14권7호
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• pp.821-829
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• 2004

얼굴인식기술이 인증 및 보안을 위한 도구로 활용되고 있지만 입력영상의 상태, 즉 조명환경에 따라 적용할 수 있는 범위가 제약적일 수밖에 없다. 본 논문에서는 이러한 제약을 최소화하기 위해 측면과 후면조명 등의 불규칙한 조명환경에서 획득한 입력영상에서 얼굴의 특징을 구분하여 얼굴영상임을 확인하는 방법을 제안한다. 제안된 방법은 에지차영상을 얼굴특징이 두드러지도록 전처리한 후, X와 Y축의 프로파일을 이용하여 얼굴영역을 예측하고 영역 내의 밝기분포를 이용하여 눈, 코, 입 등의 얼굴특징이 놓일 수 있는 수평영역을 분리한다. 수평영역들은 눈, 코, 입을 포함할 수 있는 영역의 그룹으로 나누어지고 각 그룹에서 코와 입, 그리고 눈의 순서로 특징들을 검출한다. 얼굴여부는 검출된 특징들의 구조적인 관계를 검증하여 확인한다. 제안된 알고리즘은 배경색상이나 조명의 방향과 색상 등으로 인해 얼굴의 형태와 특징이 결여된 입력영상에서도 매우 안정적으로 적용됨을 실험을 통해 확인하였다.

• 한국도로학회논문집
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• 제19권5호
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• pp.21-31
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• 2017

PURPOSES : One of the main components of road projects funded by the Economic Development Cooperation Fund (EDCF) is the improvement or rehabilitation of existing pavements. The result is that pavement structures are critical to the success of a project. There is, however, no design standard available at present that reflects a region's specific features including climate conditions and quality of pavement materials. For this reason, a comparative study of the major EDCF borrowers' flexible pavement design standards was conducted. This study led to the proposal of a new method for applying flexible pavement designs which can be used for EDCF-funded projects in Asia. METHODS : The method has been produced by adjusting some input data of the "AASHTO Interim Guide for Design of Pavement Structures" in accordance with certain Asian countries' geometrical features, tropical and subtropical weather, and strength of pavement materials. The Philippine regional factors, having five different grades, have been selected after taking into consideration the amount of rainfall, strength of pavement materials, and characteristics of the Asia and Pacific regions. Structural layer coefficients have been prepared for two different regions according to the geometric difference between Southeast and Southwest Asia. The Philippine and Sri Lankan coefficients have been used for Southeast Asia and Southwest Asia, respectively. CONCLUSIONS : Owing to applying this new method, it was verified that the thickness of the pavement was underestimated by between 11 cm and 16 cm compared with the originally designed thickness. Having discovered that the use of the Korean and American-oriented factors and coefficients is not appropriate for other Asian countries, the new method is expected to enhance the quality of pavement in future projects.

• 자원환경지질
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• 제29권3호
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• pp.335-343
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• 1996

The dipole-dipol electrical resistivity survey was conducted to investigate the probable contamination of the Han river by leachate from the near-by Nanjido Landfill. The survey line of 3 km was set along the unpaved road toward the Han river. For the convenience of the field work, the survey line was divided into four segments. The complete two-dimensional resistivity section was constructed by connecting the inversion result of each segment. Gravity survey was also carried out along the profile parallel to the resistivity line. Near surface resistivity generally appeared to be of very low value in most part of the survey area and the boundary between the alluvium layer and underlying basement rocks is well discriminated on the resistivity section. These results agree well with those of the preceding Schlumberger depth sounding made at adjacent area by Lee and fun (1995). The variation of thickness of the alluvium layer delineated by gravity anomaly profile also correlates well with the result of the resistivity survey on the qualitative basis. The problem of contamination by leachate from the Nanjido Landfill, where various waste materials have been dumped without any proper treatment facilities, has been remains unsolved yet. Therefore, we present the most probable passages of leachate flow based on the survey results and have briefly discussed about measure for contamination control. Considering the thickness of alluvium and the possible existence of fractured zone, the middle point between 1st and 2nd landfill and the midst of 1st landfill are the most hazardous regions to make leachates flow into the Han river. Since large amounts of leachates are observed from the test wells located on the lines extending from the border between the 1st and 2nd landfill and the middle of the lst landfill, contamination protection barriers are strongly recommended near these regions.

• Mycobiology
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• 제29권3호
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• pp.121-131
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• 2001

This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions(ITS1 and ITS2) and 5.8S ribosomal DNA(rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and TTS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The Geniank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an putgroup in all taxa.

• 대기
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• 제29권5호
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• pp.525-535
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• 2019

Vertical distributions of aerosol mass concentrations over Seoul and Gangneung from January to February 2015 were investigated using aerosol Mie-scattering lidars. Vertical mass concentration of aerosol was calculated from the lidar data using KALION's algorithm and quantitatively compared with ground PM₁₀ concentration to obtain objectivity of data. The backward trajectories calculated using HYSPLIT (version 4) were clustered into 5 traces for Seoul and 6 traces for Gangneung, and the observed aerosol vertical mass distribution was analyzed for individual trajectories. Result from the analysis shows that, aerosol concentrations with in the planetary boundary layer were highest when airflows into the measurement points originated in the Shandong Peninsula or the Inner Mongolia. In addition, the difference of aerosol mass concentrations in the two regions below 1 km was about twice as large as that in the long range transport from the Shandong Peninsula compared to the local emission. This result shows that the air quality over Korea related to particulate matters are affected more by aerosol emissions in the upstream source regions and the associated transboundary transports than local emissions. This study also suggests that the use of local aerosol observations is critical for accurate simulations of aerosol-cloud interactions.

• 한국비블리아학회지
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• 제26권2호
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• pp.241-268
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• 2015

'전국 도서관 운영평가'는 대통령소속 도서관정보정책위원회에서 2008년부터 매년 시행하고 있으며 거의 모든 공공도서관이 평가에 참여하고 있다. 본 연구에서는 전국도서관 운영 평가의 성과를 분석하기 위하여 제 1차 도서관발전종합계획 기간(2009년~2013년) 가운데, 동일한 평가 지표가 적용된 2010년 실적부터 2013년 실적까지 4년간의 평가 데이터를 대상으로 규모를 기준으로 한 범주별 분석과 수도권과 비수도권으로 나눈 지역 간 분석을 실시하였다. 분석 결과 계획과 장서구성 등 내부 운영 역량 개선의 측면에서는 전반적으로 긍정적 효과를 거두었으나, 수도권과 비수도권 사이의 격차는 개선되지 않았으며, 도서관 그룹 별로 투입 자원과 도달률 사이의 상관관계가 다르게 나타남을 발견하여 이를 고려한 정책 수립이 필요함을 제안하였다.

• Journal of Applied Biological Chemistry
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• 제43권1호
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• pp.5-11
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• 2000

Three cDNA clones encoding rice calmodulin (CaM) isoforms (OsCaM-1, OsCaM-2, and OsCaM-3) were isolated from a rice cDNA library constructed from suspension-cultured rice cells treated with fungal elicitor. The coding regions of OsCaM-1 and O.sCaM-2 were 89% homologous at DNA Ievel, whereas the 5' and 3' untranslated regions were highly divergent. The polypeptides encoded by OsCaM-1 and OsCaM-2 was identical except two conservative substitution at position 8 and 75. The coding region of OsCaM-3 was consist of a typical conserved CaM domain and an additional C-terminal extension. The amino acid sequence of conserved CaM domain of OsCaM-3 shared only 86% identity with that OsCaM-1. The OsCaM-3 cDNA is belongs to a novel group of calmodulin gene due to its C-terminal extension of 38 amino acids, a large number of which are positively charged. The extension also contains a C-terminal CaaX-box prenylation site (CVlL). Genomic Southern analysis revealed at least six copies of CaM or CaM-related genes, suggesting that calmodulin may be represented by a small multigene family in the rice geneme. Expression of OsCaM gene was examined through Northern blot analysis. Transcript level of OsCaM-3 was increased by treatment with a fungal elicitor, whereas the OsCaM-1 and OsCaM-2 genes did not respond to the fungal elicitor. The expression of OsCaM-3 gene was remarkable inhibited in the rice cells treated with cyclosporine A, calcinurin inhibitor.