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Comparison of Volatile Flavor Compounds of Domestic Onions Harvested in Various Regions (지역별 생산 양파종의 휘발성 향기성분 비교분석)

  • Lee, Hee-Young;Jeong, Eun-Jeong;Jeon, Seon-Young;Cho, Min-Sook;Cho, Woo-Jin;Kim, Hee-Dae;Cha, Yong-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.12
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    • pp.1609-1614
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    • 2008
  • To provide scientific information for processing of value-added products from onion, volatile flavor compounds were compared in onions harvested in 6 regions, such as Muan (Jeonnam), Buan (Jeonbuk), Andong (Gyeongbuk), and 3 regions of Gyeongnam around Changnyeong (Yueo, Jangma, Seongsan). A total of 51 compounds were detected in samples by solid phase microextraction (SPME)/GC/MSD and they consisted mainly of sulfur-containing compounds (20), aldehydes (4), ketones (5), esters (11), aromatic hydrocarbons (4), nitrogen containing compounds (2), and miscellaneous compounds (5). The sulfur-containing compounds were the major compounds with ranges of $62.6{\sim}80.3%$ of total volatiles, regardless of harvested regions. The amounts of 5 sulfur containing compounds known as having antioxidant activity (2,4-, 2,5-dimetylthiophene, 2-vinyl-1,3-dithiane, 5-methoxy thiazole, and 3,5-diethyl-1,2,4-trithiolane) were the highest in Andong and followed by Yueo, Jangma, Buan, Seongsan, and Muan. However, onions from Buan region had the highest amounts of 5 sulfur-containing compounds known as having anticarcinogenic activity ((Z)-, (E)-methyl propenyl disulfide, (Z)-, (E)-propenyl propyl disulfide, and di-2-propenyl disulfide), followed by Yueo, Andong, Jangma, Seongsan, and Muan.

Cultural Conditions for Mycelial Growth and Molecular Phylogenetic Relationship in Different Wild Strains of Schizophyllum commune

  • Alam, Nuhu;Cha, Youn-Jeong;Shim, Mi-Ja;Lee, Tae-Soo;Lee, U-Youn
    • Mycobiology
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    • v.38 no.1
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    • pp.17-25
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    • 2010
  • The common split-gilled mushroom, Schizophyllum commune is found throughout the world on woody plants. This study was initiated to evaluate conditions for favorable vegetative growth and to determine molecular phylogenetic relationship in twelve different strains of S. commune. A suitable temperature for mycelial growth was obtained at $30^{\circ}C$. This mushroom grew well in acidic conditions and pH 5 was the most favorable. Hamada, glucose peptone, Hennerberg, potato dextrose agar and yeast malt extract were favorable media for growing mycelia, while Lilly and glucose tryptone were unfavorable. Dextrin was the best and lactose was the less effective carbon source. The most suitable nitrogen sources were calcium nitrate, glycine, and potassium nitrate, whereas ammonium phosphate and histidine were the least effective for the mycelial growth of S. commune. The genetic diversity of each strain was investigated in order to identify them. The internal transcribed spacer (ITS) regions of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 129 to 143 bp and 241 to 243 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, while the 5.8S sequences were identical. A phylogenetic tree of the ITS region sequences indicated that the selected strains were classified into three clusters. The reciprocal homologies of the ITS region sequences ranged from 99 to 100%. The strains were also analyzed by random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. Twelve primers efficiently amplified the genomic DNA. The number of amplified bands varied depending on the primers used or the strains tested. The average number of polymorphic bands observed per primer was 4.5. The size of polymorphic fragments was obtained in the range of 0.2 to 2.3 kb. These results indicate that the RAPD technique is well suited for detecting the genetic diversity in the S. commune strains tested.

Authentication of Traded Traditional Medicine Ogapi Based on Nuclear Ribosomal DNA Internal Transcribed Spacers and Chloroplast DNA Sequences (nrDNA ITS 및 엽록체 DNA 염기서열 분석에 의한 유통 한약재 오가피 판별)

  • Kim, Jeong Hun;Byeon, Ji Hui;Park, Hyo Seop;Lee, Jeong Hoon;Lee, Sang Won;Cha, Sun Woo;Cho, Joon Hyeong
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.6
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    • pp.489-499
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    • 2015
  • Background : Plants belonging to 5 species of the genus Eleutherococcus are currently distributed in the Korean peninsula. The traditional medicine 'Ogapi', derived from Eleutherococcus sessiliflorus and other related species, and 'Gasiogapi', derived from Eleutherococcus senticosus, are frequently mixed up and marketed. Therefore, accurated identification of their origins in urgently required. Methods and Results : Candidate genes from nuclear ribosomal DNA (nrDNA) and chloroplast DNA (cpDNA) of Eleutherococcus plants were analyzed. Whereas the nrDNA-internal transcribed spacer (ITS) regions were useful in elucidating the phylogenetic relationships among the plants, the cpDNA regions were not as effective. Therefore, a combined analysis with nrDNA-ITS was performed. Various combinations of nrDNA and matK were effective for discriminating among the plants. However, the matK and rpoC1 combination was ineffective for discriminating among some species. Based on these results, it was found that OG1, OG4, OG5, OG7, GS1, GS2, and GS3 were derived from E. sessiliflorus. In particular, it was confirmed that GS1, GS2, and GS3 were not derived from E. senticosus. However, more samples need to be analyzed because identification of the origins of OG2, OG3, OG6 and GS4 was not possible. Conclusion : The ITS2, ITS5a, and matK combination was the most effective in identifying the phylogenetic relationship among Eleutherococcus plants and traditional medicines based on Eleutherococcus.

Robust Real-time Face Detection Scheme on Various illumination Conditions (다양한 조명 환경에 강인한 실시간 얼굴확인 기법)

  • Kim, Soo-Hyun;Han, Young-Joon;Cha, Hyung-Tai;Hahn, Hern-Soo
    • Journal of the Korean Institute of Intelligent Systems
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    • v.14 no.7
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    • pp.821-829
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    • 2004
  • A face recognition has been used for verifying and authorizing valid users, but its applications have been restricted according to lighting conditions. In order to minimizing the restricted conditions, this paper proposes a new algorithm of detecting the face from the input image obtained under the irregular lighting condition. First, the proposed algorithm extracts an edge difference image from the input image where a skin color and a face contour are disappeared due to the background color or the lighting direction. In the next step, it extracts a face region using the histogram of the edge difference image and the intensity information. Using the intensity information, the face region is divided into the horizontal regions with feasible facial features. The each of horizontal regions is classified as three groups with the facial features(including eye, nose, and mouth) and the facial features are extracted using empirical properties of the facial features. Only when the facial features satisfy their topological rules, the face region is considered as a face. It has been proved by the experiments that the proposed algorithm can detect faces even when the large portion of face contour is lost due to the inadequate lighting condition or the image background color is similar to the skin color.

A Method for Customizing Flexible Pavement Design Parameters for EDCF-Funded Projects in Asia (아시아 지역 EDCF 사업의 가요성포장 설계 계수 적용방안)

  • Shim, Cha-Sang;Cho, Yoon-Ho
    • International Journal of Highway Engineering
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    • v.19 no.5
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    • pp.21-31
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    • 2017
  • PURPOSES : One of the main components of road projects funded by the Economic Development Cooperation Fund (EDCF) is the improvement or rehabilitation of existing pavements. The result is that pavement structures are critical to the success of a project. There is, however, no design standard available at present that reflects a region's specific features including climate conditions and quality of pavement materials. For this reason, a comparative study of the major EDCF borrowers' flexible pavement design standards was conducted. This study led to the proposal of a new method for applying flexible pavement designs which can be used for EDCF-funded projects in Asia. METHODS : The method has been produced by adjusting some input data of the "AASHTO Interim Guide for Design of Pavement Structures" in accordance with certain Asian countries' geometrical features, tropical and subtropical weather, and strength of pavement materials. The Philippine regional factors, having five different grades, have been selected after taking into consideration the amount of rainfall, strength of pavement materials, and characteristics of the Asia and Pacific regions. Structural layer coefficients have been prepared for two different regions according to the geometric difference between Southeast and Southwest Asia. The Philippine and Sri Lankan coefficients have been used for Southeast Asia and Southwest Asia, respectively. CONCLUSIONS : Owing to applying this new method, it was verified that the thickness of the pavement was underestimated by between 11 cm and 16 cm compared with the originally designed thickness. Having discovered that the use of the Korean and American-oriented factors and coefficients is not appropriate for other Asian countries, the new method is expected to enhance the quality of pavement in future projects.

Dipole-Dipole Resistivity Survey on the Side of Han River near Nanjido Landfill (난지도에 인접한 한강변에서의 쌍극자-쌍극자 전기탐사)

  • Lee, Kiehwa;Kwon, Byung-Doo;Oh, Seok-Hoon;Kim, Cha-Seop
    • Economic and Environmental Geology
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    • v.29 no.3
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    • pp.335-343
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    • 1996
  • The dipole-dipol electrical resistivity survey was conducted to investigate the probable contamination of the Han river by leachate from the near-by Nanjido Landfill. The survey line of 3 km was set along the unpaved road toward the Han river. For the convenience of the field work, the survey line was divided into four segments. The complete two-dimensional resistivity section was constructed by connecting the inversion result of each segment. Gravity survey was also carried out along the profile parallel to the resistivity line. Near surface resistivity generally appeared to be of very low value in most part of the survey area and the boundary between the alluvium layer and underlying basement rocks is well discriminated on the resistivity section. These results agree well with those of the preceding Schlumberger depth sounding made at adjacent area by Lee and fun (1995). The variation of thickness of the alluvium layer delineated by gravity anomaly profile also correlates well with the result of the resistivity survey on the qualitative basis. The problem of contamination by leachate from the Nanjido Landfill, where various waste materials have been dumped without any proper treatment facilities, has been remains unsolved yet. Therefore, we present the most probable passages of leachate flow based on the survey results and have briefly discussed about measure for contamination control. Considering the thickness of alluvium and the possible existence of fractured zone, the middle point between 1st and 2nd landfill and the midst of 1st landfill are the most hazardous regions to make leachates flow into the Han river. Since large amounts of leachates are observed from the test wells located on the lines extending from the border between the 1st and 2nd landfill and the middle of the lst landfill, contamination protection barriers are strongly recommended near these regions.

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Phylogenetic Analysis of Caterpillar Fungi by Comparing ITS 1-5.8S-ITS 2 Ribosomal DNA Sequences

  • Park, Joung-Eon;Kim, Gi-Young;Park, Hyung-Sik;Nam, Byung-Hyouk;An, Won-Gun;Cha, Jae-Ho;Lee, Tae-Ho;Lee, Jae-Dong
    • Mycobiology
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    • v.29 no.3
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    • pp.121-131
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    • 2001
  • This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions(ITS1 and ITS2) and 5.8S ribosomal DNA(rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and TTS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The Geniank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an putgroup in all taxa.

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Characteristics of Vertical Profiles of Local Aerosol Mass Concentration According to Air Mass Pathways over the Korean Peninsula During Winter (한반도 겨울철 공기이동경로에 따른 에어로졸 농도의 연직분포 특성)

  • Ko, A-Reum;Kim, Jinwon;Chang, Ki-Ho;Cha, Joo-Wan;Lee, Sang-Min;Ha, Jong-Cheol
    • Atmosphere
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    • v.29 no.5
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    • pp.525-535
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    • 2019
  • Vertical distributions of aerosol mass concentrations over Seoul and Gangneung from January to February 2015 were investigated using aerosol Mie-scattering lidars. Vertical mass concentration of aerosol was calculated from the lidar data using KALION's algorithm and quantitatively compared with ground PM10 concentration to obtain objectivity of data. The backward trajectories calculated using HYSPLIT (version 4) were clustered into 5 traces for Seoul and 6 traces for Gangneung, and the observed aerosol vertical mass distribution was analyzed for individual trajectories. Result from the analysis shows that, aerosol concentrations with in the planetary boundary layer were highest when airflows into the measurement points originated in the Shandong Peninsula or the Inner Mongolia. In addition, the difference of aerosol mass concentrations in the two regions below 1 km was about twice as large as that in the long range transport from the Shandong Peninsula compared to the local emission. This result shows that the air quality over Korea related to particulate matters are affected more by aerosol emissions in the upstream source regions and the associated transboundary transports than local emissions. This study also suggests that the use of local aerosol observations is critical for accurate simulations of aerosol-cloud interactions.

A Study on the Results of the National Evaluation on Public Library Management During 2010~2013 (전국 공공도서관 운영평가의 성과에 관한 연구 - 2010년~2013년도를 중심으로 -)

  • Cha, Mikyeong;Pyo, Soon Hee
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.26 no.2
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    • pp.241-268
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    • 2015
  • Since 2008, the Presidential Committee on Library and Information has conducted 'National Library Management Evaluation,' and almost all of the public libraries have participated in the evaluation. In order to find the effect of the Evaluation on the public libraries, this study analyzed four years of the performance scores (2010~2013) during the period of the $1^{st}$ National Library Development Plan 2009~2013, which used the same measurement, were analyzed by the size groups and regions. The results indicated positive effect on the quality of the internal process such as planning and collection building. Yet attainment gab remains between metropolitan and the other regions, and also different relationship exists between input and attainment among the public library size groups. It suggested the policy development considering the library size and the regional difference.

Molecular Characterization of Three cDNA Clones Encoding Calmodulin Isoforms of Rice

  • Lee, Sung-Ho;Kim, Cha Young;Lim, Chae Oh;Lee, Soo In;Gal, Sang Wan;Choi, Young Ju
    • Journal of Applied Biological Chemistry
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    • v.43 no.1
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    • pp.5-11
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    • 2000
  • Three cDNA clones encoding rice calmodulin (CaM) isoforms (OsCaM-1, OsCaM-2, and OsCaM-3) were isolated from a rice cDNA library constructed from suspension-cultured rice cells treated with fungal elicitor. The coding regions of OsCaM-1 and O.sCaM-2 were 89% homologous at DNA Ievel, whereas the 5' and 3' untranslated regions were highly divergent. The polypeptides encoded by OsCaM-1 and OsCaM-2 was identical except two conservative substitution at position 8 and 75. The coding region of OsCaM-3 was consist of a typical conserved CaM domain and an additional C-terminal extension. The amino acid sequence of conserved CaM domain of OsCaM-3 shared only 86% identity with that OsCaM-1. The OsCaM-3 cDNA is belongs to a novel group of calmodulin gene due to its C-terminal extension of 38 amino acids, a large number of which are positively charged. The extension also contains a C-terminal CaaX-box prenylation site (CVlL). Genomic Southern analysis revealed at least six copies of CaM or CaM-related genes, suggesting that calmodulin may be represented by a small multigene family in the rice geneme. Expression of OsCaM gene was examined through Northern blot analysis. Transcript level of OsCaM-3 was increased by treatment with a fungal elicitor, whereas the OsCaM-1 and OsCaM-2 genes did not respond to the fungal elicitor. The expression of OsCaM-3 gene was remarkable inhibited in the rice cells treated with cyclosporine A, calcinurin inhibitor.

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