• Title/Summary/Keyword: region-specific materials

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Molecular Authentication of Morus Folium Using Mitochondrial nad7 Intron 2 Region

  • Jin, Chi-Gyu;Kim, Min-Kyeung;Kim, Jin-Young;Sun, Myung-Suk;Kwon, Woo-Saeng;Yang, Deok-Chun
    • Korean Journal of Plant Resources
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    • v.26 no.3
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    • pp.397-402
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    • 2013
  • Morus Folium (Sang-yeop in Korean) is one of the most important Oriental medicinal plants. In Korea, both M. alba and M. cathayana are regarded as the botanical sources for Morus Folium. In order to discriminate M. alba and M. cathayana from their adulterant, M. tricuspidata, mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 2 region was targeted for molecular analysis with universal primers. DNA polymorphisms, including SNP sites, insertions, and deletions, were detected among these three species sequencing data. Based on these DNA polymorphisms, specific primers were designed for the three species respectively. Multiplex PCR was conducted for molecular authentication of M. alba, M. cathayana, and M. tricuspidata with specific primers. The present results indicate that it is possible to identify Morus Folium from its adulterant using mitochondrial nad7 intron 2 region. The established multiplex-PCR system was proved to be effective for identification of Morus Folium. The results indicate that mitochondrial introns can be used for inter-specific polymorphic study, and the described method can be applied for molecular identification of medicinal materials.

A Study on the Physical Characteristics of Steel-Wire Sound Absorbing Materials (금속와이어 흡음재의 물리적 특성에 관한 연구)

  • 주경민;이동훈;용호택
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2002.05a
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    • pp.1244-1249
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    • 2002
  • In this study, the physical characteristics of steel-wire sound absorbing materials with different thickness and bulk density is experimentally obtained in terms of the porosity and specific flow resistivity. Based on the experimental results, the following conclusions can be made. The porosities of steel-wire sound absorbing materials are smaller than those of general absorbing materials, which are inversely proportional to the volume densities. For the porosity measurement with a good accuracy, the dynamic correction based on the system compliance should be involved in porosity measurement. In addition, the flow condition for the precise measurement of the specific flow resistivity of steel-wire sound absorbing materials should be limited in the laminar flow region.

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Feasibility for Ultrasound Pad Material for the Evaluation Axillary Region of Automated Breast Ultrasound Equipment (자동유방초음파 장비의 액와부 평가를 위한 초음파 패드 물질의 타당성)

  • Seo, Eun-Hee;Seoung, Youl-Hun
    • Journal of radiological science and technology
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    • v.41 no.3
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    • pp.231-240
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    • 2018
  • Automated breast ultrasound (ABUS) equipment is a new innovative technique for 3D automatic breast scanning, but limited for the examination in the concave axillary region. The purpose of this study was to determine feasible candidate materials for the ultrasonic wave propagation media in ABUS, enabling the evaluation of the axillary region. Ultrasonography was performed using an ABUS system ($Invenia^{TM}ABUS$, GE, USA) on the ultrasound-specific phantom (UC-551M-0.5, ATS Laboratories, USA) covered by different candidate materials. The validity of feasible candidate materials was evaluated by image quality. Three independent radiological technologists, with more than 10 years of experience, visually assessed on the images. The inter-observer agreements according to the candidate materials were tested using Cronbach's alpha. Unenveloped solidified carrageenan can be a feasible material for the use of ABUS with excellent test reliability. Therefore, the coverage of the axillary region with carrageenan may be effective for ABUS which was originally developed for the convex anatomic structure as female breast.

IR Absorption Property in Nano-thick Ir-inserted Nickel Silicides (이리듐이 첨가된 니켈실리사이드의 적외선 흡수 특성)

  • Yoon, Kijeong;Song, Ohsung;Han, Jeungjo
    • Korean Journal of Metals and Materials
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    • v.46 no.11
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    • pp.755-761
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    • 2008
  • We fabricated thermally evaporated 10 nm-Ni/1 nm-Ir/(poly)Si films to investigate the energy saving property of silicides formed by rapid thermal annealing (RTA) at the temperature range of $300{\sim}1200^{\circ}C$ for 40 seconds. Moreover, we fabricated 100 nm-thick ITO/(poly)Si films with an rf-sputter as references. A transmission electron microscope (TEM) and an X-ray diffractometer were used to determine cross-sectional microstructure and phase changes. A UV-VIS-NIR and FT-IR (Fourier transform infrared spectroscopy) were employed for near-IR and middle-IR absorbance. Through TEM analysis, we confirmed 20~65 nm-thick silicide layers formed on the single and polycrystalline silicon substrates. Ir-inserted nickel silicide on single crystalline substrate showed almost the same absorbance in near IR region as well as ITO, but Ir-inserted nickel silicide on polycrystalline substrate, which had the uniform absorbance in specific region, showed better absorbance in near IR region than ITO. The Ir-inserted nickel silicide on polycrystalline substrate particularly showed better absorbance in middle IR region than ITO. The results imply that nano-thick Ir-inserted nickel silicides may have excellent absorbing capacity in near-IR and middle-IR region.

Analysis of Hemocyte-specific Gene Expression from Bombyx mori

  • Park, Seung-Won;Goo, Tae-Won;Kim, Seong-Ryul;Kang, Seok-Woo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.23 no.1
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    • pp.137-141
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    • 2011
  • A previous data was provided information for tissuespecific expression genes by means of whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the hemocyte tissue on 5 days of 5th instar larvae during the development of $B.$ $mori$. Total 5 candidates pick out from the $Bombyx$ $mori$ Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray). To verify the hemocyte-specific expression, we analyzed by semi-quantitative and real-time quantitative RT-PCR using the highly expressed endogenous $Actin$ RNA as an intrinsic reference. In this study, we confirmed that one gene-sw17255- out of 5 candidates expressed in the hemocyte tissue, which was consistent with the previous data. Circulating hemocytes in the body fluid of the $B.$ $mori$ are most powerful target organ for producing biomaterials. We need further studies to find hemocyte-specific promoter region from sw17255 gene. Finally, this result can be applied in creating transgenic silkworms as a biomedical insect.

Analysis of Variation of Specific Cutting Resistance in Nanoscale Cutting (나노스케일 절삭가공에서의 비절삭저항 변화 및 원인 분석)

  • Kwon, Ye-Pil;Kim, Si-Hoon;Jeon, Eun-chae
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.19 no.11
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    • pp.23-28
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    • 2020
  • In general, lithography techniques are applied when machining single-crystal silicon in nanoscale applications; however, these techniques involve low degrees of freedom for the vertical shapes. By applying mechanical techniques to machine silicon, nanopatterns having various types of vertical shapes can be manufactured. In this study, we determined the ductile-brittle machining transition point and analyzed the- variation of the specific cutting resistance within the ductile machining region in nanoscale applications. When brittle fracture occurred during the nanoscale cutting, the depth of cut and cutting force increased and decreased rapidly, respectively. The first point of rapid increase in the depth of cut was defined as the ductile-brittle machining point. Subsequently, the shape of the machining tool was observed using a scanning electron microscope to calibrate the machining area, considering the tip blunting. The specific cutting resistance decreased continuously and converged to a certain value during the nanoscale cutting. The decrease and convergence in the value can be attributed to the decrease in the ratio of the arc length to the area of the machining tool and silicon.

A Framework for Development of Correctness Centered e-Learning based Curriculum in Sukkur Region

  • Ahmed Masood Ansari;Mumtaz H. Mahar
    • International Journal of Computer Science & Network Security
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    • v.23 no.6
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    • pp.13-16
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    • 2023
  • This study aims to explore the status of e-learning in the public sector institutes of the Sukkur region in Pakistan. A survey was conducted to collect data from students and teachers regarding their awareness, access, and use of e-learning resources. The results showed that although there is a widespread use of the internet and mobile devices for accessing information, there is a lack of awareness and access to e-learning resources. Barriers to accessing e-learning content and a lack of familiarity with e-learning content development technologies were also identified. The study concludes that there is a need for improved e-learning facilities and curriculum in the public sector institutes of the Sukkur region in Pakistan. Recommendations are provided for developing a correctness-centered e-learning based curriculum that is tailored to the specific needs of the students in the region. It is hoped that the findings of this study will inform efforts to improve the teaching and learning process in the region and provide students with greater flexibility and access to study materials.

The three-dimensional microstructure of trabecular bone: Analysis of site-specific variation in the human jaw bone

  • Kim, Jo-Eun;Shin, Jae-Myung;Oh, Sung-Ook;Yi, Won-Jin;Heo, Min-Suk;Lee, Sam-Sun;Choi, Soon-Chul;Huh, Kyung-Hoe
    • Imaging Science in Dentistry
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    • v.43 no.4
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    • pp.227-233
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    • 2013
  • Purpose: This study was performed to analyze human maxillary and mandibular trabecular bone using the data acquired from micro-computed tomography (micro-CT), and to characterize the site-specific microstructures of trabeculae. Materials and Methods: Sixty-nine cylindrical bone specimens were prepared from the mandible and maxilla. They were divided into 5 groups by region: the anterior maxilla, posterior maxilla, anterior mandible, posterior mandible, and mandibular condyle. After the specimens were scanned using a micro-CT system, three-dimensional microstructural parameters such as the percent bone volume, bone specific surface, trabecular thickness, trabecular separation, trabecular number, structure model index, and degrees of anisotropy were analyzed. Results: Among the regions other than the condylar area, the anterior mandibular region showed the highest trabecular thickness and the lowest value for the bone specific surface. On the other hand, the posterior maxilla region showed the lowest trabecular thickness and the highest value for the bone specific surface. The degree of anisotropy was lowest at the anterior mandible. The condyle showed thinner trabeculae with a more anisotropic arrangement than the other mandibular regions. Conclusion: There were microstructural differences between the regions of the maxilla and mandible. These results suggested that different mechanisms of external force might exist at each site.

Receptor-Mediated Endocytosis of Hepatitis B Virus PreS1d Protein in EBV-Transformed B-Cell line

  • Park, Jung-Hyun;Cho, Eun-Wie;Lee, Dong-Gun;Park, Jung-Min;Lee, Yun-Jung;Choi, Eun-A;Kim, Kill-Lyong
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.844-850
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    • 2000
  • The specific binding and internalization of viral particles is an essential step for the successful infection of viral pathogens. In the case of the hepatitis B virus (HBV), virions bind to the host cell via the preS domain of the viral surface antigen and are subsequently internalized by endocytosis. HBV-preS specific receptors are primarily expressed on hepatocytes, however, viral DNA and proteins have also been detected in extrahepatic sites, suggsting that celluar recepators for HBV may also exist on extrahepatic cells. Recently, an EBV-transformed B-cell line was identified onto which the preS region binds in a receptor-ligand specific manner. In this study, this specific interaction was further characterized, and the binding region within the preS protein was locaized. Also the internalization after host cell attachment was visualized and analyzed by fluorescence-labeled HBV-preS1 proteins using confocal microscopy. Energy depletion by sodium azide treatment effectively inhibited the internalization of the membrane-bound preS1 ligands, thereby indicating an energy-dependent receptor-mediated endocytotic pathway. Accordingly, the interaction of HBV-pres! with this specific B-cell line may serve as an effective model for an infection pathway in extrahepatic cells.

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Study on the Characteristics of Cavitation Erosion-Corrosion for Mild Steel ( 1 ) - Damage Behaviour of Vibration Cavitation Erosion-Corrosion - (연강의 캐비테이션 침식-부식 특성에 관한 연구 ( 1 ) - 진동 캐비네이션 침식-부식 손상 거동 -)

  • Lim, Un-Joh;Hwang, Jae-Ho
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.31 no.4
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    • pp.413-422
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    • 1995
  • Cavitation erosion-corrosion implies damage to materials due to the shock pressure or shock wave that results when bubbles form and collapse at a metal surface within a liquid. If the liquid is corrosive to the material, a condition typically encountered in industry, the component materials may suffer serious damage by a combination of mechanical and electrochemical attack. In this study, the mild steel(SS41) was tested by using the piezoelectric vibrator with 20kHz, 24$\mu$m to cavity generation apparatus. The damage behaviour of vibration cavitation erosion-corrosion and the environment characteristics were investigated in various solutions which are seawater, tap water and distilled water. The main results obtained are as follows; 1) The cavitation erosion-corrosion damage behaviour in the seawater increases to occur at the equal degree on the middle portion and the outside portion of specimen. The distilled water specimen, on other hand, occurs beginning on the outside portion across to the middle portion of specimen. 2) The cavitation erosion-corrosion damage in the tap water of low specific resistance more increases than that in the distilled water of high specific resistance at the initial testing time and more decreases than that in it by the CaCO sub(3) film with testing time. 3) Cavitation erosion-corrosion damage characteristic divides into four regions; incubation region, acceleration region, deceleration region and steady state region.

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