• Journal of Ocean Engineering and Technology
• /
• v.34 no.4
• /
• pp.263-271
• /
• 2020

In this study, we performed a three-dimensional (3D) topology optimization of a fixed offshore structure to enhance its structural stiffness. The proposed topology optimization is based on the solid isotropic material with penalization (SIMP) method, where a volume constraint is applied to utilize an equivalent amount of material as that used for the rule-based scantling design. To investigate the effects of the main legs of the fixed offshore structure on its structural stiffness, the leg region is selectively considered in the design domain of the topology optimization problem. The obtained optimal designs and the rule-based scantling design of the structure are manufactured by 3D metal printing technology to experimentally validate the topology optimization. The behaviors under compressive loading of the obtained optimal designs are compared with those of the rule-based scantling design using a universal testing machine (UTM). Based on the structural experiments, we concluded that by employing the topology optimization method, the structural stiffness of the structure was enhanced compared to that of the rule-based scantling design for an equal amount of the fabrication material. Furthermore, by effectively combining the topology optimization and rule-based scantling methods, we succeeded in enhancing the structural stiffness and improving the breaking load of the fixed offshore structure.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.24 no.4
• /
• pp.307-314
• /
• 2004

A circumferential guided wave method was developed to detect the axial crack on the bent feeder pipe. Dispersion curves of circumferential guided waves were calculated as a function of curvature of the pipe. In the case of thin plate, i.e. infinite curvature, as the frequency increases, the $S_0$ and $A_0$ mode coincide and eventually become Rayleigh wave mode. In the case of pipe, however, as the curvature increases, the lowest modes do not coincide even in the high frequencies. Based on the analysis, a rocking technique using angle beam transducer was applied to detect an axial defect in the bent region of PHWR feeder pipe. Based on the analysis of experimenal data for artificial notches, the vibration modes of each signal were identified. It was found that the notches with the depth of )0% of wall thickness can be detected with the method.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.11
• /
• pp.1662-1669
• /
• 2007

The high mobility group AT-hook1 (HMGA1) proteins are known to be related to the regulation of gene transcription, replication and promotion of metastatic progression in cancer cells. The loss of expression by disrupting the HMGA1 gene affects insulin signaling and causes diabetes in the mouse. Previously identified single nucleotide polymorphism (SNP) of HMGA1 was significantly associated with fat deposition traits in the pig. In this study, we identified 3,935 bp nucleotide sequences from exon 5 to exon 8 of the bovine HMGA1 gene and its mRNA expression was observed by quantitative real-time PCR. Six single nucleotide polymorphisms in the bovine HMGA1 gene were detected and the allele frequencies of these SNPs were investigated using the PCR-RFLP method in nine cattle breeds including Limousin, Simmental, Brown Swiss, Hereford, Angus, Charolais, Hanwoo, Brahman and Red Chittagong cattle. The map location showed that the bovine HMGA1 gene was also closely located with a previously identified meat quality QTL region indicating this gene is the most likely positional candidate for meat quality traits in cattle.

• Reproductive and Developmental Biology
• /
• v.31 no.4
• /
• pp.227-233
• /
• 2007

This study was conducted to examine the mRNA expression of apoptosis-related and imprinted genes and methylation pattern of the differentially methylated region (DMR) of H19 gene in day 35 of SCNT pig fetuses. The day 35 of natural mating (control) or cloned (clone) pig fetuses were recovered from uterus. Endometrium from dam and liver from fetus were obtained, respectively. mRNA expression was evaluated by real-time PCR and methylation pattern was analyzed by bisulfite sequencing method. The Bcl-2 mRNA expression in clone was significantly lower than that of control (p<0.05). The mRNA expression of H19 gene in both endometrium and liver was significantly higher in clone than that of control, respectively (p<0.05). The level of IGF-2 mRNA in liver of clone was significantly lower than that of control (p<0.05), whereas the mRNA expression of IGF2-R gene in liver of clone was significantly higher than that of control (p<0.05). The DMR of H19 was lower methylation pattern in clone than that of control. These results suggest that the aberrant mRNA expression of apoptosis-related and imprinted genes and the lower DMR methylation pattern of imprinted gene may be closely related to the inadequate fetal development of cloned fetus.

• Journal of Animal Science and Technology
• /
• v.53 no.6
• /
• pp.511-516
• /
• 2011

Fas gene known to associate with intramuscular fat content in Korean cattle was selected for DNA marker development. Fas (APO-1, CD95), a member of the tumor necrosis factor (TNF) receptor superfamily, is a cell membrane protein that mediates apoptosis (programmed cell death). We discovered single nucleotide polymorphisms (SNPs) within Fas gene in order to develop novel DNA markers at genomic level. Of this gene to search for SNP, sequences of whole exon and 1kb range of both front and back of the gene using 24 cattle were determined by direct-sequencing methods. As a result, 16 SNPs in exon, 37 SNPs in intron and 2 SNPs in promoter region, a total of 55 SNPs were discovered. In these SNPs, thirty-one common polymorphic sites were selected considering their allele frequencies, haplotype-tagging status and Linkage Disequilibrium (LD) for genotyping in larger-scale subjects. Selected SNPs were confirmed genotype through SNaPshot method (n=274) and were examined for possible genetic association of Fas polymorphisms with carcass weight (CWT), eye muscle area (EMA), and backfat thickness (BF). So, the SNP have been identified significant g.-12T>G, g.1112T>G and g.32548T>C. These results suggest that polymorphism of Fas gene was associated with meat quality traits in Hanwoo.

• Journal of Korean Society of Forest Science
• /
• v.112 no.3
• /
• pp.322-330
• /
• 2023

Amid the recent intensification of climate change, the decline of subalpine coniferous species is evident. Accordingly, the Korea Forest Service is conducting research to establish conservation measures, identify the causes of the decline of subalpine coniferous species, and establish conservation strategies for seven species of conifers in the alpine region. This study used an economic value evaluation method to present objective data to inform the need for and value of conservation of endangered coniferous species through the recognition of endangered coniferous species and evaluation of conservation value among coniferous species conservation strategies in endangered alpine areas. A contingent valuation method was applied. As a result, the estimated conservation value per household of endangered alpine coniferous species was 49,181 won.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.11 no.6
• /
• pp.3188-3207
• /
• 2017

In this paper, we propose an image enhancement algorithm to improve license plate extraction rate in various environments (Day Street, Night Street, Underground parking lot, etc.). The proposed algorithm is composed of image enhancement algorithm and license plate extraction algorithm. The image enhancement method can improve an image quality of the degraded image, which utilizes a histogram information and overall gray level distribution of an image. The proposed algorithm employs an interpolated probability distribution value (PDV) in order to control a sudden change in image brightness. Probability distribution value can be calculated using cumulative distribution function (CDF) and probability density function (PDF) of the captured image, whose values are achieved by brightness distribution of the captured image. Also, by adjusting the image enhancement factor of each part region based on image pixel information, it provides a function that can adjust the gradation of the image in more details. This processed gray image is converted into a binary image, which fuses narrow breaks and long thin gulfs, eliminates small holes, and fills gaps in the contour by using morphology operations. Then license plate region is detected based on aspect ratio and license plate size of the bound box drawn on connected license plate areas. The images have been captured by using a video camera or a personal image recorder installed in front of the cars. The captured images have included several license plates on multilane roads. Simulation has been executed using OpenCV and MATLAB. The results show that the extraction success rate is more improved than the conventional algorithms.

• Journal of Ginseng Research
• /
• v.41 no.1
• /
• pp.31-35
• /
• 2017

Background: Korean ginseng (Panax ginseng) is a well-known medicinal plant of Oriental medicine that is still in practice today. Until now, a total of 11 Korean ginseng cultivars with unique features to Korean ginseng have been developed based on the pure-line-selection method. Among them, a new cultivar namely G-1 with different agricultural traits related to yield and content of ginsenosides, was developed in 2012. Methods: The aim of this study was to distinguish the new ginseng cultivar G-1 by identifying the unique single-nucleotide polymorphism (SNP) at its 45S ribosomal DNA and Panax quinquefolius region than other Korean ginseng cultivars using multiplex amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR). Results: A SNP at position of 45S ribosomal DNA region between G-1, P. quinquefolius, and the other Korean ginseng cultivars was identified. By designing modified allele-specific primers based on this site, we could specifically identified G-1 and P. quinquefolius via multiplex PCR. The unique primer for the SNP yielded an amplicon of size 449 bp in G-1 cultivar and P. quinquefolius. This study presents an effective method for the genetic identification of the G-1 cultivar and P. quinquefolius. Conclusion: The results from our study shows that this SNP-based approach to identify the G-1 cultivar will be a good way to distinguish accurately the G-1 cultivar and P. quinquefolius from other Korean ginseng cultivars using a SNP at 45S ribosomal DNA region.

• Journal of the Korea Society of Computer and Information
• /
• v.24 no.1
• /
• pp.73-83
• /
• 2019

In this paper, we propose the augmented reality(AR) based driving navigation based on robust lane detection method to dynamic environment changes. The proposed technique uses the detected lane position as a marker which is a key element for enhancing driving information. We propose Symmetrical Local Threshold(SLT) algorithm which is able to robustly detect lane to dynamic illumination environment change such as shadows. In addition, by using Morphology operation and Connected Component Analysis(CCA) algorithm, it is possible to minimize noises in the image, and Region Of Interest(ROI) is defined through region division using a straight line passing through several vanishing points We also propose the augmented reality aided visualization method for Interchange(IC) and driving navigation using reference point detection based on the detected lane coordinates inside and outside the ROI. Validation experiments were carried out to assess the accuracy and robustness of the proposed system in vairous environment changes. The average accuracy of the proposed system in daytime, nighttime, rainy day, and cloudy day is 79.3% on 4600 images. The results of the proposed system for AR based IC and driving navigation were also presented. We are hopeful that the proposed research will open a new discussion on AR based driving navigation platforms, and thus, that such efforts will enrich the autonomous vehicle services in the near future.

• Transactions on Electrical and Electronic Materials
• /
• v.10 no.2
• /
• pp.53-57
• /
• 2009

In this paper we develop a method to observe faults in semiconductor devices and transmission lines by calculating the variation of the reflection function in a dielectric microstrip line that has an open-ended termination containing an optically induced plasma region. It is analyzed with the assumption that the plasma is distributed homogeneously in laser illumination. With the non linear material of degradation, the concentration of the carrier in the part of the material has changed. Since the input wave has produced the phenomenon of reflection, the input signal to the open-ended microstrip lines can be observed on reflection to identify the location of the fault. The characteristic impedances resulting from the presence of plasma are evaluated by the transmission line model. The variation of the reflection wave in the microwave system has been calculated by using an equivalent circuit model. The transient response has been also evaluated theoretically for changing the phase of the variation in the reflection. The variation of characteristic response in differentially localized has been also evaluated analytically.