• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.21.1-21.11
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• 2016

Background: The evaluation of suitable reference genes as normalization controls is a prerequisite requirement for launching quantitative reverse transcription-PCR (RT-qPCR)-based expression study. In order to select the stable reference genes in abalone Haliotis discus hannai tissues (gill and hepatopancreas) under heavy metal exposure conditions (Cu, Zn, and Cd), 12 potential candidate housekeeping genes were subjected to expression stability based on the comprehensive ranking while integrating four different statistical algorithms (geNorm, NormFinder, BestKeeper, and ${\Delta}CT$ method). Results: Expression stability in the gill subset was determined as RPL7 > RPL8 > ACTB > RPL3 > PPIB > RPL7A > EF1A > RPL4 > GAPDH > RPL5 > UBE2 > B-TU. On the other hand, the ranking in the subset for hepatopancreas was RPL7 > RPL3 > RPL8 > ACTB > RPL4 > EF1A > RPL5 > RPL7A > B-TU > UBE2 > PPIB > GAPDH. The pairwise variation assessed by the geNorm program indicates that two reference genes could be sufficient for accurate normalization in both gill and hepatopancreas subsets. Overall, both gill and hepatopancreas subsets recommended ribosomal protein genes (particularly RPL7) as stable references, whereas traditional housekeepers such as ${\beta}-tubulin$ (B-TU) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were ranked as unstable genes. The validation of reference gene selection was confirmed with the quantitative assay of MT transcripts. Conclusions: The present analysis showed the importance of validating reference genes with multiple algorithmic approaches to select genes that are truly stable. Our results indicate that expression stability of a given reference gene could not always have consensus across tissue types. The data from this study could be a good guide for the future design of RT-qPCR studies with respect to metal regulation/detoxification and other related physiologies in this abalone species.

• 한국통신학회:학술대회논문집
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• 한국통신학회 2004년도 하계종합학술발표회논문초록집
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• pp.179-179
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• 2004

• 한국한의학연구원논문집
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• 제3권1호
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• pp.17-40
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• 1997

${\ulcorner}$Classified Collection of Medical Prescriptions${\lrcorner}$(${\ulcorner}$醫方類聚${\lrcorner}$ is a medical book which classifies and edits the existing books. First of all, it is not until finishing the study of reference books that we can study the ${\ulcorner}$Classified Collection of Medical Prescriptions${\lrcorner}$ in earnest. We did not take traditional non-systematic methods to study the reference books of ${\ulcorner}$Classified Collection of Medical Prescriptions${\lrcorner}$ systematically. We investigated the title of 'reference book's name' as a reference book and did not investigate re-reference books. The number of reference books are one hundred forty-two volumes. Most of the reference books are medical books; some books are about Taoism and Buddhism. The title of the reference books are given a name by various methods. But these methods have a lot of problems. First, the same title book misapply an alias, the original text name and an abbreviated name. Second, a chapter name misapplies reference books. Third, reference books misapplies a chapter name. Forth, the writer and book name misapplies reference books and so on. From quotation collection about ${\ulcorner}$Classified Collection of Medical Prescriptions${\lrcorner}$'s characteristic point fellows below in three kinds. First, it emphasizes medical therapy. Second, most of Song(宋) period, Geum Yuan(金 元) period of China reference books take place in here. During this period it had accepted studying theory from clinical accumulation and the result of re-clinical studies reception based on theory research, and also had accepted Geum Yuan's expansive theory. Third, by adding technical books of a professional assortment, it has raised its profession of division.

• 한국문헌정보학회지
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• 제32권2호
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• pp.121-142
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• 1998

온라인정보기술 환경이 참고봉사에 어떤 특성과 변화를 가져오는지를 구체적으로 검토하였다. 선행연구 결과를 바탕으로 고찰하였으며, 경우에 따라 국내 도서관의 사례조사도 실시하였다. 다음으로 이러한 특성과 변화가 이용자에 대한 참고사서의 기본적 중재역할인 정보제공, 교육, 상담, 평가 기능에 어떻게 영향을 미치게되는지 분석하였다. 변화된 상황에서 참고사서의 사명에 대한 개념적 기반을 보다 명백히하고 새롭게 확인된 역할을 실행하기 위해 필요한 자질을 재고해 보는데 본 논고의 궁극적 의의가 있다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.740-743
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• 1997

Reference models are used in many control algorithms for improvement of transient response characteristics. They provide desired trajectories that the plant should follow. Most control systems have bounded control inputs to avoid saturation of the plant. If we design reference models that do not account for limits of control inputs, control performance of the system may be deteriorated. In this paper, therefore, the way of determining variable reference models for TDC(time delay control) technique is proposed. The variable reference model is determined based on the information of bounded control inputs and reference inputs. This proposed method is also verified by application to the position control experiment using the BLDC motor.

• 대한기계학회논문집A
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• 제28권6호
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• pp.701-708
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• 2004

This paper discusses applicability of the enhanced reference stress method to estimate J-integral along the semi-elliptical surface crack front. It is found that angular variations of normalized J­integral are strongly dependent on the geometry, loading mode and loading magnitude. As application of the reference stress approach to semi-elliptical surface cracks implies proportional increases in the normalized J-integral, the present results pose a question in applicability of the reference stress approach. However, investigation of the error in the estimated J-integral in the present work suggests that the enhanced reference stress approach, recently proposed by authors, provides an effective engineering tool fur estimating crack driving force along the semi-elliptical surface crack front.

• 한국공작기계학회논문집
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• 제15권5호
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• pp.72-78
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• 2006

The paper proposes reference model error feedback control scheme for motion control system with hard non-linear components as like saturation and dead-zone in plant input part. Additionally, the plant has the system uncertainty effected by plant model parameter deviation and disturbance. The control algorithm uses the reference model to apply additional feedback loop with the error between reference model output and actual output effected by disturbance and non-linear components. And the stability evaluation based on Popov stability and controller design method are formulated to be performed. The effectiveness of the proposed scheme is examined by simulations. The results are proven by reasonable performances following reference model responses with good disturbance rejection performance without over-tuning of controller.

• 한국문헌정보학회지
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• 제3권
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• pp.25-46
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• 1973

The recent situation on developments in an expanding field of reference and information service is reviewed through the cooperative arrangements and comprehensive planning among libraries in America. The growth of reference cooperation is examined first along with the social forces that influenced the library interdependence for service. Of the various interlibrary programs identified in relation to the cooperative reference work, interlibrary loan is viewed as the key to the use of library resources based on equal access and making use of the tools for information and research, therefore a reference principle. Several kinds of library systems specifically intended to improve the reference situation are identified and described in detail without any attempt to evaluate their performance in terms of unit size, cost, or quality of the service.

• The International Journal of Costume Culture
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• 제2권1호
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• pp.77-86
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• 1999

This study was designed to identify the information sources of reference used by male college students. Theoretical framework is based upon social comparison theory, reference group theory, and Kotler's model of personal and non-personal sources of reference. The subjects were 287 male college students. The questionnaire were distributed to the school area and the commercial area such as Shinchon, Myung-Dong, and Apkujung-Dong in Seoul. Means, standard deviation, frequency, T-test, one-way ANOVA, and Duncan's multiple range test were applied for the analyses of data. The result of hyporthesis 1 showed that male college student utilize personal and non-personal sources of reference indiscriminately. The result of hypothesis 2 showed that male college students consult the socially high-proximal group such as a girl friend(a lover) and mother most actively. The result of hypothesis 3 found that male college students use position media most actively, especially in-store displays, followed by print media and broadcast media.

• Transactions on Control, Automation and Systems Engineering
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• 제3권1호
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• pp.32-42
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• 2001

In this paper, a parameter estimator is developed for the plant model whose structure is represented by the Takagi-Sugeno model. The essential idea behind the on-line estimation is the comparison of the measured stated with the state of an estimation model whose structure is the same as that of the parameterized model. Based on the parameter estimation scheme, and indirect Model Reference Adaptive Fuzzy control(MRAFC) scheme is proposed to provide asymptotic tracking of a reference signal for the systems with uncertain for slowly time-varying parameters. The developed control law and adaptive law guarantee the boundedness of all signals in the closed-loop systems. In addition, the plant state tracks the state of the reference model asymptotically with time for any bounded reference input signal.