• Journal of Life Science
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• v.29 no.11
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• pp.1179-1191
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• 2019

Cancer cells undergo the epithelial-mesenchymal transition (EMT) and show unique oncogenic metabolic phenotypes such as the glycolytic switch (Warburg effect) which are important for tumor development and progression. The EMT is a critical process for tumor invasion and metastasis. High-mobility group box 1 (HMGB1) is a chromatin-associated nuclear protein, but it acts as a damage-associated molecular pattern molecule when released from dying cells and immune cells. HMGB1 induces the EMT, as well as invasion and metastasis, thereby contributing to tumor progression. Here, we show that HMGB1 induced the EMT by activating Snail. In addition, the HMGB1/Snail cascade was found induce a glycolytic switch. HMGB1 also suppressed mitochondrial respiration and cytochrome c oxidase (COX) activity by a Snail-dependent reduction in the expression of the COX subunits COXVIIa and COXVIIc. HMGB1 also upregulated the expression of several key glycolytic enzymes, including hexokinase 2 (HK2), phosphofructokinase-2/fructose-2,6-bisphosphatase 2 (PFKFB2), and phosphoglycerate mutase 1 (PGAM1), in a Snail-dependent manner. However, HMGB1 was found to regulate some other glycolytic enzymes including lactate dehydrogenases A and B (LDHA and LDHB), glucose transporter 1 (GLUT1), and monocarboxylate transporters 1 and 4 (MCT1 and 4) in a Snail-independent manner. Transfection with short hairpin RNAs against HK2, PFKFB2, and PGAM1 prevented the HMGB1-induced EMT, indicating that glycolysis is associated with HMGB1-induced EMT. These findings demonstrate that HMGB1 signaling induces the EMT, glycolytic switch, and mitochondrial repression via Snail activation.

• Journal of Korea Water Resources Association
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• v.49 no.6
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• pp.459-467
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• 2016

LISFLOOD-FP model in which channel flows are resolved separately from the floodplain flows using either a kinematic or diffusive wave approximation has been used to analyze flooding behavior on the lower Mankyung River influenced by backwater. A calibration and validation process was applied using the previous flood events to assess the model performance. Sensitivity analysis was conducted for main calibrated parameters, such as Manning roughness coefficient and downstream boundary condition. Also, we examined the effect of warm-up for the initial conditions. The results show that the computed hydrograph is in good agreement with measured data on the study reach, even though it was a hydrologic kinematic wave model. The sensitive analysis show that the difference between the computed results may be greater depending on the used calibrated parameters and that the sufficient calibration/validation process against various flood events is necessary. If the flood inundation simulation is performed using the validated model, it is expected to be able to contribute about river planning and policy decision-making for flood damage reduction.

• Tunnel and Underground Space
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• v.33 no.5
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• pp.388-398
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• 2023

The changes in groundwater flow due to mining development act as a contributing factor to major issues such as ground subsidence, strength reduction and collapse. For the sustainable mining development, measures for dealing with fluctuations in seasonal underground water inflow, power losses, pump damage, and unexpected increases in inflow must be put in place. In this study, the aim is to identify the causes of underground seepage through the examination of hydrological connectivity between the study area and nearby limestone mine. A tracer tes for assessing subsurface connectivity has been planned. A variety of tracers, such as dyes and ions, were applied in lab test to select the optimal tracer material, and a hydrological model of the study area was implemented through field test. Finally, the hydrological connectivity between the external stream and underground water in the mine was analyzed.

• Journal of the Korean Society for Marine Environment & Energy
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• v.14 no.3
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• pp.154-162
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• 2011

Seawater quality was investigated each month at 30 stations near Tongyeong, South Korea, to provide data for the effective use of coastal fisheries and the reduction of economic damage to marine products. Water temperature was lowest in January and highest at the end of August. Neither extremely low water temperature below $4^{\circ}C$ nor fish damage caused by low water temperature was observed. Salinity ranged from 24.04 to 34.39 psu in the surface layer and from 29.92 to 34.39 psu in the bottom layer. The minimum salinity, attributable to rainfall events, was observed in July; salinity increased to high of about 34 psu in November. Low dissolved oxygen (DO), below 4 mg/L, was observed at Wenmun and Buksin Bays during May to October. Concentrations of $NO_2$-N, $NO_3$-N, and $PO_4$-P were low from March to September and high from October to February. Transparency was 6 m on average and was high in Wenmun Bay. Chemical oxygen demand (COD) and chlorophyll a (Chl. a) were high during summer, when the water temperature was high. With cluster analysis based on environment factors related to water quality, the study area could be divided into three main sea areas: Buksin Bay, coastal seawater, and offshore seawater. Buksin Bay was characterized by low salinity, high DO and Chl. a, and high transparency in the surface layer and by low DO and high $NH_4$-N in the bottom layer. Offshore seawater had high salinity and $NO_3$-N and low Chl. a concentration. In summer season that oyster need lots of phytoplankton, $NO_3$-N and Chl. a concentrations at this study area were low compare to Gwangy-ang and Gamak Bays. In winter, a sea squirt swallow much more than other season, the Chl. a concentrations were also low than Gwangyang and Gamak Bays.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.371-371
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• 2019

우리나라는 홍수기(6~9월)에 집중되는 기상패턴과 하천 중하류부에 발달된 도시의 개발특성으로 인하여 가장 중요한 자연재해 중 하나로 홍수 및 도시침수가 거론되고 있다. 과거 집중호우로 침수 피해가 발생한 사례를 살펴보면, 피해가 발생하는 지역은 지방하천 및 소하천을 중심으로 형성된 도시지역이다. 중앙 지방 정부는 수차례 침수 피해를 겪으며 사후관리가 아닌 재난예방 및 사전관리 등의 방안 마련을 강조하고 있다. 하지만 기후변화에 의한 기상의 불확실성으로 치수 중심의 물관리 및 중 소하천의 하천 특성으로 여전히 홍수 발생에 대비할 수 있는 골든타임 확보 등에 어려움을 겪고 있다. 이러한 어려움을 극복하기 위해 사전 예방적 차원에서의 홍수대응 방안으로 중 소하천을 담당하는 지자체 중심의 홍수피해 저감 방안이 필요하다. 본 연구에서는 A 지자체를 대상으로 모니터링 대상 경계를 설정하여 우량 알람 기준을 예비알람, 주요 관측지점에 대해 강우에 따른 수위 상승 정도를 홍수대응 기준인 직접알람과 연계함으로써 예방적 재난대응 체계를 구축하였다. 모니터링 대상 지역은 해당 지자체를 포함하면서 유역 개념을 적용하여 만경강유역 전체로 설정하였다. 만경강 유역 내 유관기관(지자체, 환경부, K-water, 기상청 등)이 관할하는 우량국(41개소) 및 수위국(28개소), 저수용량이 30만톤 이상이 되는 농어촌공사 저수지(7개소)를 고려하여 홍수분석 모형(COSFIM)을 구축하였다. 해당 모형은 2018년 8월 호우사상에 대해 주요 수위관측 지점에서 $R^2$가 0.8 이상의 우수한 검증 결과를 보였다. 구축된 모형을 통해 예상강우량별 하천 내 수위지점별 최고수위, 최대유량, 도달시간 등 예상 조견표를 제시하여 호우 발생시 지자체 담당자가 참고할 수 있도록 제시하였다. 또한 수위지점별 홍수대응 기준은 평시, 관심, 주의, 경계, 심각 단계로 구분하여 담당자가 수위별 위험 정도를 인지할 수 있도록 지점별 도달되는 수위의 위험 정보를 알람기준으로 제시하였다. 홍수분석 모형은 상류에 위치한 주요시설물의 운영현황을 연계하고 있어 실제 강우 발생 시 기상예보를 고려하여 하천 내 수위관측 지점별 수위 상승 정도를 예상함으로써 사전에 홍수에 대비할 수 있는 단계별 시간 확보에 활용 가능하다. 향후 홍수대응기준은 하천 환경 변화를 반영하여 지속적인 보완이 필요하며 유관기관과의 수문자료 공유체계 확대로 예방적 차원의 홍수 대응 체계가 구축되어야 할 것이다.

• Journal of Korean Society of Disaster and Security
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• v.12 no.4
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• pp.1-13
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• 2019

Recently, as the occurrence frequency of sudden floods due to climate change increased, it is necessary to install the facilities that can cope with the initial stormwater. Most researches have been conducted on the design of facilities applying the Low Impact Development (LID) and the reduction effect on rainfall runoff to examine with 1D or 2D numerical models. However, the studies on the examination about flow characteristics and stability of pipe network systems were relatively insufficient in the literature. In this study, the stability of the pipe network system in rainwater storage tank was examined by using 3D numerical model, FLOW-3D. The changes of velocity and dynamic pressure were examined according to the number of rainwater storage tank and compared with the design criteria to derive the optimal design plan for a rainwater storage tank. As a results of numerical simulation with the design values in the previous study, it was confirmed that the velocity became increased as the number of rainwater storage tank increased. And magnitude of the velocity in pipes was formed within the design criteria. However, the velocity in the additional rainwater storage pipe was about 3.44 m/s exceeding the allowable range of the design criteria, when three or more additional rainwater storage tanks were installed. In the case of turbulence intensity and bottom shear stress, the bottom shear stress was larger than the critical shear stress as the additional rainwater storage was increased. So, the deposition of sediment was unlikely to occur, but it should be considered that the floc was formed by the reduction of the turbulence intensity. In addition, the dynamic pressure was also satisfied with the design criteria when the results were compared with the allowable internal pressure of the pipes generally used in the design of rainwater storage tank. Based on these results, it was suitable to install up to two additional rainwater storage tanks because the drainage becomes well when increasing of the number of storage tank and the velocity in the pipe becomes faster to be vulnerable to damage the pipe. However, this study has a assumption about the specifications of the rainwater storage tanks and the inflow of stormwater and has a limitation such that deriving the suitable rainwater storage tank design by simply adding the storage tank. Therefore, the various storage tank types and stormwater inflow scenarios will be asked to derive more efficient design plans in the future.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1304-1308
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• 2006

The present study describes the preliminary evaluation of the antioxidant activity and the cytotoxic effect of Ceramium kondoi. The antioxidant activities and cytotoxic effect of the water extracts were evaluated by total phenolic contents (TPC), DPPH radical scavenging activity (RSA), reducing power (RP), comet assay, and MTT reduction assay. TPC, DPPH RSA, and RP of the extract at the concentration of $1,000{\mu}g/mL$ was $659.2{\mu}M$, 86.0%, and 1.084, respectively, and those were concentration dependent. The $200{\mu}M\;H_2O_2-induced$DNA damage was inhibited by C. kondoi water extract in a dose dependent manner in human leukocytes. The inhibition was by 62.3, 39.8, 24.8% and 16.4% at the concentration of 5, 10, $25{\mu}g/mL$ and $50{\mu}g/mL$, respectively. Cytotoxic activity on HT-29 cells and MCF-7 cells of the C. kondoi water extract at the concentration of $10{\mu}g/mL$ was 49% and 60%, respectively. These results strongly support the possibility of C. kondoi as a source of natural functional materials.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.6
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• pp.815-824
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• 2022

Among the main facilities of the power plant, the circulating water used for cooling the power generation system is supplied through the Circulation Water Intake Basin (CWIB). The vortexes of various types generated in the Pump Sump (PS) of CWIB adversely affect the Circulation Water Pump (CWP) and pipelines. In particular, the free surface vortex accompanied by air intake brings about vibration, noise, cavitation etc. and these are the causes of degradation of CWP performance, damage to pipelines. Then power generation is interrupted by the causes. Therefore, it is necessary to investigate the hydraulic characteristics of CWIB through the hydraulic model experiment and apply an appropriate Anti Vortex Device (AVD) that can control the vortex to enable smooth operation of the power plant. In general, free surface vortex is controlled by Curtain Wall (CW) and the submerged vortex is by the anti vortex device of the curtain wall. The detailed specifications are described in the American National Standard for Pump Intake Design. In this study, the circulating water intake part of the Tripoli West 4×350 MW power plant in Libya was targeted, the actual operating conditions were applied, and the vortex reduction effect of the anti vortex device generated in the suction tank among the circulating water intake part was analyzed through a hydraulic model experiment. In addition, a floor splitter was basically applied to control the submerged vortex, and a new type of column curtain wall was additionally applied to control the vortex generated on the free surface to confirm the effect. As a result of analyzing the hydraulic characteristics by additionally applying the newly developed Column Curtain Wall (CCW) to the existing curtain wall, we have found that the vortex was controlled by forming a uniform flow. In addition, the vortex angle generated in the circulating water pump pipeline was 5° or less, which is the design standard of ANSI/HI 9.8, confirming the stability of the flow.

• Korean Journal of Poultry Science
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• v.40 no.1
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• pp.11-16
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• 2013

Salmonella is closely related with human health of modern society which has concern increased in livestock goods consumption as well as give economic damage throughout the chicken industry such as farm, hatchery, slaughter house and processing plant. From 2007 to 2011, this study investigated Salmonella prevalence from 200 old hen delivery trucks which deliver old egg-laying hens and broiler breeders in Korea. The prevalence of Salmonella species was 38.0% in old hen delivery truck. Serogroup C1 was the most frequently detected serogroup of Salmonella, followed by the serogroups D1, C2 and B. A total of 25 serotypes were identified and Salmonella infantis was the most frequently isolated serotype. In addition, we applied disinfectant to old hen delivery truck for the reduction of Salmonella contamination. The disinfectant consists of formaldehyde, glutaladehyde and quaternary ammonium compound was applied to the trucks. Salmonella isolation rate was significantly decreased after disinfection from 38.0% to 7.5%. Disinfectant could not effectively reduce Salmonella contamination at a dilution of 1:200 which is recommended by manufacturer, but Salmonella isolation rate was significantly decreased at a dilution of 1:50. Since old hen delivery truck could be a potential vector to carry Salmonella into farms and abattoirs, chicken delivery truck should be disinfected thoroughly and sufficiently to control contamination of farms and abattoirs.

• Korean Journal of Environmental Agriculture
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• v.6 no.2
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• pp.53-65
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• 1987

The study was performed to investigate the effect of gaseous emissions of sulfur dioxide and hydrogen fluoride on the growth of rice plants under stressed field conditions consisting of 88 industrial plants operating with 285 smoke stacks emitting pollutants. As for the relationship between yields and yield components it is believed that the panicles per hill is the single most important component affecting the rate of yield of the rice plant. Based on the standard partial regression coefficient analysis, panicles per hill has the largest contribution to yield and the average contribution of 54%. Other components such as spikelets per panicle, percent fertility and 1000 grain weight are also contributing factors to yield, although far less so. Fluorine content in the leaf appear to have more negative effect on panicles per hill, percent fertility and subsequent overall yield than does sulfur content in the leaf. It is constantly observed and interesting to note that fluorine and sulfur content in the leaf appears to have no effect on spikelets per panicle and 1000 grain weight. Reduction in yield seems to be affected mainly by panicles per hill which are, in turn, affected more by fluorine content in the leaf as demonstrated by the standard partial coefficient analysis. Regarding the prediction sum of the square of the regression equation, the lowest value was found when nine variables were used for the analysis. The variables taken into consideration were the monthly sulfur and fluorine content in the leaf as well as the monthly percent of leaf damage during the months of June, July and August. A significant correlation is found between the actual and predicted yields by the regression equations selected as a result of a prediction sum of the square analysis.