• ALGAE
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• v.22 no.2
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• pp.95-106
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• 2007

Blooms of Chattonella species are normally during summer in inland seas with high nutrients from the land and inflowing water. These blooms cause mass fish kills worldwide. We isolated a Chattonella strain from the south coast of Korea and identified it as C. antiqua. It is known that the morphological changes of phytoplankton correspond to the diurnal vertical migrations that follow an intrinsic biological clock and a nutrient acquisition mechanism during the day and night. In electron micrographs, C. antiqua clearly showed a radial distribution of lipid bodies in subcellular regions and plastids composed in which thylakoid layers were perpendicular to the surface. A single pyrenoid was present in each plastid and it was found at the end of the plastid towards the center of the cell. Throughout the day, plastids of C. antiqua cells appeared as an expanded net-like recticulum. During the night, however, the plastids changed their shape and contracted toward the cell periphery. The electron density of pyrenoids was increased in cells harvested during the night.

• Smart Media Journal
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• v.5 no.1
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• pp.130-136
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• 2016

In this paper, the real time monitoring system for the abnormal state of marine algae does not detect the plankton which may directly cause the red tide or the water bloom. But checks both oxygen reduction and nitrogen reduction in water, which indicates the characteristics of zooplankton and phytoplankton respectively, and this system makes a module that monitors in real time the temperature and the illumination of the water, which are indirect factors, with sensors placed in and outside the water, and this module transmits signals periodically at specific intervals to a sever that builds up data base, and the data collected in these ways will be analyzed and compared with the standard data from Ministry of Oceans and Fisheries, and then these data will be made the adequate form of information to be provided to the users as visual information, thus, this system intends to make a red tide and water bloom monitoring system tailored for individual fish farm businesses that has local characteristics and can quickly operate outside working hours, which differs from the existing wide area detecting and monitoring systems.

• The Korean Journal of Malacology
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• v.26 no.1
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• pp.19-31
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• 2010

Seogundo is a small island adjacent to the southern coast of Jeju Island and connected to it by a boulder beach at low tide Surveys of this area were conducted from 2001 to 2009 to enumerate the mollusks there and also to examine their diversity, relative abundance, and ecological relationships. Both the boulder beach itself and several large tide pools were studied, including the coarse sand substrate and several species of seaweed and coralline algae found in the tide pools. Of the 121 species obtained or observed, there were 97 gastropods, 16 bivalves, and 8 polyplacophorans. Live specimens were obtained for about half of those species. About one third were found on rocky substrate, with the most common species being Nodilittorina radiata and Nerita japonica in the upper intertidal zone, N. radiata and Littorina brevicula in the middle intertidal, and Turbo (Lunella) coronata coreensis and Acanthopleura japonica in the lower intertidal and shallow subtidal. The seaweeds and coralline algae contained about 40% of all mollusk species. The most common mollusks in two species of brown seaweed were Ittibittum parcum, Musculus nanus, and Euplica scripta. In a species of red seaweed, Komaitrochus pulcher was the most frequent, as in the coralline algae, along with M. nanus. The coarse sand in the tidepools contained about 25% of the species, with the Cerithiidae having the largest number. A sample of beach drift contained 17 species, with Bittium aleutaceum and Rissoina (Phosinella) pura being most common. Most species, about 60%, were found in a variety of habitats, especially the marine flora; few species exhibited any habitat preferences. Biographically, Jeju Island is part of the Warm Temperate Northwest Pacific Province and the East China Sea ecoregion with a strong faunal affinity with southern Japan, eastern China, and northeastern Taiwan. Zonal-geographical groupings reveal that the fauna is mainly subtropical-low boreal, preferring moderately warm water, with a somewhat smaller number of tropical-subtropical species.

• ALGAE
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• v.29 no.2
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• pp.153-163
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• 2014

Mesodinium rubrum is a cosmopolitan ciliate that often causes red tides. Predation by heterotrophic protists is a critical factor that affects the population dynamics of red tide species. However, there have been few studies on protistan predators feeding on M. rubrum. To investigate heterotrophic protists grazing on M. rubrum, we tested whether the heterotrophic dinoflagellates Gyrodiniellum shiwhaense, Gyrodinium dominans, Gyrodinium spirale, Luciella masanensis, Oblea rotunda, Oxyrrhis marina, Pfiesteria piscicida, Polykrikos kofoidii, Protoperidinium bipes, and Stoeckeria algicida, and the ciliate Strombidium sp. preyed on M. rubrum. G. dominans, L. masanensis, O. rotunda, P. kofoidii, and Strombidium sp. preyed on M. rubrum. However, only G. dominans had a positive growth feeding on M. rubrum. The growth and ingestion rates of G. dominans on M. rubrum increased rapidly with increasing mean prey concentration < $321ngCmL^{-1}$, but became saturated or slowly at higher concentrations. The maximum growth rate of G. dominans on M. rubrum was $0.48d^{-1}$, while the maximum ingestion rate was 0.55 ng C $predator^{-1}d^{-1}$. The grazing coefficients by G. dominans on populations of M. rubrum were up to $0.236h^{-1}$. Thus, G. dominans may sometimes have a considerable grazing impact on populations of M. rubrum.

• The Journal of the Acoustical Society of Korea
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• v.24 no.8
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• pp.447-453
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• 2005

Laboratory measurements were performed in a uni-algae medium Cochlodinium polykrikoides (Phytoplankton, dinoflagellates) using an Underwater Ultrasound $(5\~15\;MHz)$ to study Characteristics of Acoustic Backscattering of Harmful algae. In an effort to detect the harmful algal scatterers with population density of less than 300 cells/ml that corresponds to the precaution stage of red tide, backscattered signals from various scatterer-density samples were obtained and analyzed. Correlations between volume backscattering strength (Sv) and population density (cells/ml) of scatterers in the medium have been investigated. Comparison of Volume Backscattering Strengths calculated with the fluid-sphere model [1] and the measured values showed an agreement.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.508-514
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• 2017

Water-bloom and red tide due to eutrophication have been overgrown and have caused various environmental problems. Recently, however, research on bid-diesel that can utilize algae as an energy source has been actively carried out. In particular, many studies variously have been conducted to utilize algal photosynthesis oxygen as a supply method for reducing the energy by an air blower in MWTP. In this study, a lab scale algae-nitrification reactor was operated to replace the oxygen required for nitrogen removal and the operation period was largely divided into three sections. In the first section, ammonia nitrogen removal efficiency was 24 ~ 38% according to the MLSS (Mixed Liquer Suspended Solid) concentration. In the second section, ammonia nitrogen removal efficiency was 38 ~ 50% according to the micro-algae concentration and in the last section ammonia nitrogen removal efficiency was 61 ~ 80% according to HRT (Hydraulic Retention Time). As a result, as the MLSS decreased and algae biomass increased, the ammonia nitrogen removal efficiency tended to increase, but the effect of Algae biomass was greater than that of MLSS.

• ALGAE
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• v.31 no.4
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• pp.373-378
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• 2016

Cochlodinium polykrikoides is a red-tide forming dinoflagellate that causes significant worldwide impacts on aquaculture industries and the marine ecosystem. There have been extensive studies on managing and preventing C. polykrikoides blooms, but it has been difficult to identify an effective method to control the bloom development. There is also limited genome information on the molecular mechanisms involved in its various ecophysiology and metabolism processes. Thus, comprehensive genome information is required to better understand harmful algal blooms caused by C. polykrikoides. We estimated the C. polykrikoides genome size using flow cytometry, with detection of the fluorescence of DNA stained with propidium iodide (PI). The nuclear genome size of C. polykrikoides was 100.97 Gb, as calculated by comparing its mean fluorescence intensity (MFI) to the MFI of Mus musculus, which is 2.8 Gb. The exceptionally large genome size of C. polykrikoides might indicate its complex physiological and metabolic characteristics. Our optimized protocol for estimating the nuclear genome size of a dinoflagellate using flow cytometry with PI can be applied in studies of other marine organisms.

• ALGAE
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• v.38 no.1
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• pp.39-55
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• 2023

Exploring mixotrophy of dinoflagellate species is critical to understanding red-tide dynamics and dinoflagellate evolution. Some species in the dinoflagellate genus Karenia have caused harmful algal blooms. Among 10 Karenia species, the mixotrophic ability of only two species, Karenia mikimotoi and Karenia brevis, has been investigated. These species have been revealed to be mixotrophic; however, the mixotrophy of the other species should be explored. Moreover, although K. mikimotoi was previously known to be mixotrophic, only a few potential prey species have been tested. We explored the mixotrophic ability of Karenia bicuneiformis, Karenia papilionacea, and Karenia selliformis and the prey spectrum of K. mikimotoi by incubating them with 16 potential prey species, including a cyanobacterium, diatom, prymnesiophyte, prasinophyte, raphidophyte, cryptophytes, and dinoflagellates. Cells of K. bicuneiformis, K. papilionacea, and K. selliformis did not feed on any tested potential prey species, indicating a lack of mixotrophy. The present study newly discovered that K. mikimotoi was able to feed on the common cryptophyte Teleaulax amphioxeia. The phylogenetic tree based on the large subunit ribosomal DNA showed that the mixotrophic species K. mikimotoi and K. brevis belonged to the same clade, but K. bicuneiformis, K. papilionacea, and K. selliformis were divided into different clades. Therefore, the presence or lack of a mixotrophic ability in this genus may be partially related to genetic characterizations. The results of this study suggest that Karenia species are not all mixotrophic, varying from the results of previous studies.

• ALGAE
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• v.24 no.2
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• pp.105-110
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• 2009

The rDNAs of figh-killing dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum were detected from the East China Sea by species-specific real-time PCR probes. Sequence analysesusing the partial ITS sequences from the real-time PCR products showed identical sequences with C. Polykrikoides and K. veneficum, respectively and low expectation values (E-value) of less than 1e-5 suggesting the presence of these organisms in the East Ching Sea shelf water that flows into the Tsushima Strait and the Yellow Sea.

• Journal of Environmental Science International
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• v.11 no.3
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• pp.149-154
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• 2002

This study was conducted to find the structure of phytoplankton community in the Hoeya river(Ulsan) from March 2000 to February 2001. The taxa of phytoplankton identified included 135 species, 5 divisions, 33 families and 82 genera. Among them, chrysophyta(diatoms) were 63 species(46.7%), chlorophyta(green algae) 47 species(34.8%), pyrrophyta(dinoflagellates) 13 species(9.6%), cyanophyta (cyanobacteria) 8 species(5.9%) and euglenophyta(euglenoids) 4 species(3.0%) respectively. Ecological important species is called frequently appearing species, red tide causative species, seawater species and pollution indicator. 22 species including Aulacoseira granulate were recorded frequently appearing species. 19 species including Ceratium furca were recorded as red tide causing species. And the pollution indicators were 33 species including Actinastrum hantzschii var. fluviatile. The highest standing crops were 3,103,441 cells/$\ell$ in August at the RW-1 and the lowest 1,245 cells/ $\ell$ in January at the RW-5. In the community analysis, the dominanance indices ranged from 0.34(October, RW-2) to 0.94(January, RW-1) and the diversity indices from 0.50(May, RW-2) to 2.57(September, RW-2). The saprobic indices were 2.43 in RW-1, 2.41 in RW-2, 2.375 in RW-3, 2.40 in RW-4, 2.43, in RW-5. Therefore, these areas were investigated "$\beta$-mesosaprobic". According to the similarity index among the stations, these areas were defined as residential district areas(RW-2 and RW-3), lower part of the dam (RW-1) and seawater areas(RW-4 and RW-5).