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http://dx.doi.org/10.4490/ALGAE.2009.24.2.105

Detection of Fish Killing Dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum (Dinophyceae) in the East China Sea by Real-time PCR  

Park, Tae-Gyu (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Kang, Yang-Soon (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Park, Young-Tae (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Bae, Heon-Meen (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Lee, Yoon (Marine Ecology Research Division, National Fisheries Research and Development Institute)
Publication Information
ALGAE / v.24, no.2, 2009 , pp. 105-110 More about this Journal
Abstract
The rDNAs of figh-killing dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum were detected from the East China Sea by species-specific real-time PCR probes. Sequence analysesusing the partial ITS sequences from the real-time PCR products showed identical sequences with C. Polykrikoides and K. veneficum, respectively and low expectation values (E-value) of less than 1e-5 suggesting the presence of these organisms in the East Ching Sea shelf water that flows into the Tsushima Strait and the Yellow Sea.
Keywords
Cochlodinium polykrikoides; East China Sea; Karlodinium veneficum; real-time PCR; red tide;
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