• Korean Journal of Ichthyology
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• v.30 no.3
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• pp.131-136
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• 2018

Decrease in seawater temperature during winter is one of the most important and serious issues confronted by fish net-cage aquaculture farms. This can become the cause of the manifestation of diseases and ensuing mass mortality. The present study was conducted to assess the survival rate, the range of limited low-temperature, the response of oxidative stress in the blood of red sea bream Pagrus major with decrease of water temperature. Low-lethal temperature for 7 days of P. major ($7day-LT_{50}$) was $6.54^{\circ}C$ (confidence limit: $6.31{\sim}6.71^{\circ}C$). Oxygen consumption rate was decreased with lower temperature. It showed the minimum value at the range of low-lethal temperature. Osmolality at $5^{\circ}C$ and $6^{\circ}C$ experimental group was higher significantly than control group. SOD (superoxide dismutase) activity was increased significantly at $5^{\circ}C$ experimental group compared to control group. This study data will be used to determine the appropriate area for aquaculture of red sea bream. It also manage fish net-cage farm to cope with the mass mortality occurring frequently during winter season.

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.340-341
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• 2006

• Fisheries and Aquatic Sciences
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• v.26 no.2
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• pp.97-104
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• 2023

CD96 is a membrane-bound receptor discovered in humans in 1992 that is mainly present in natural killer cells and T cells derived from haematopoietic cells and performs immune functions. Based on the sequence of CD96 obtained from red seabream (Pagrus major), phylogenetic analysis with other species, infections of normal fish, Streptococcus iniae and red sea bream iridovirus (RSIV), and expression analysis was conducted using real-time polymerase chain reaction. Phylogenetic analysis showed the highest homology with Sparus aurata, and multiple sequence analysis confirmed the conservation of major domains between different fish species. Normal fish high expression results were confirmed in the head kidney, and spleen, which are the haematopoietic organs of the fish. High expression levels were confirmed in the gills, liver, spleen, and kidney on day three after RSIV infection. After S. iniae infection, high expression was confirmed in the gills and liver on day one, and high expression was confirmed in the spleen from 12 hours. These results show that PmCD96 functions as an immune gene in P. major and is considered a basic research case for CD96 in fish's hematopoietic organ immune system.

• Korean Journal of Ichthyology
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• v.29 no.1
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• pp.41-51
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• 2017

To understand the application in farm for the fish aquaculture, we investigated biological and pathological traits on red sea bream Pagrus major which were reared in each copper-alloy net cage and the synthetic fiber net cage for 9 months. Two groups of cage were made and set in Yokji-eup, Tongyoung, Gyeongsangnam-do in size of 25 m in diameter and 10 m of depth. Survival rate of the red sea bream in the rearing copper-alloy net cage and synthetic fiber cage showed 99.75% and 99.70% respectively, there was no significant difference. Daily weight growth rate in each net was shown to 2.13 g/day and 1.65 g/day. Health analysis by blood composition analysis showed a favorable result in the copper-alloy net cage rather than in the synthetic fiber net. Bioaccumulation of heavy metal such as Cu and Zn especially in gonad was higher than other organ. Bioaccumulation of Cu and Zn in the muscle was lower compared to the permitted standard for food safety. Pathogenic infection test discovered Microcotyle tai for parasite, V. alginolyticus and other five species for bacteria. But there was a little bit difference of bacteria infection in copper-alloy net cage and copper-alloy net cage is expected to be has antibacterial effect. Thus, copper-alloy net cage can be applied to farm considering its system stability, recycling, antibiosis and food safety.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.2
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• pp.109-117
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• 1997

In order to hold the behavior of fish school to the set-net, a series of tag-recapture experiments were carried out in two fishing grounds of the middle sized set-nets which were located in 20m depth on the coast of Keojedo and Namhaedo in the Southern part of Korea from September to October in 1996. In the experiments, the leading ability of the leader and the fish court and the recapturing ability of the bag nets were checked out for the six species of fish in method of discharging the tagged fishes at side points of leader, and the middle points of the fish court and the bag nets in a hauling step, and recapturing them at the bag nets in the next hauling. The results obtained are as follows; 1. The ratio recaptured at the both side bag-nets in the next hauling after discharged from the fish court in the previous step was 20.3% in small size of mackerel Scomber japonicus, 16.2% in small size of horse mackerel Trachurus japonicus, 10.3% in black sea-bream Acanthopagrus schlegelii, 19.1% in red barracuda Syhyraena pinguis, 16.3% in halfbeak Hemiramphus sajori, 20.0% in gizzard shad Konosirus punctatus individually, and totally in six species of fish, that was 17.2%. 2. The ratio recaptured at the same bag net after discharged in the both side bag-nets was 21.7% in small size of mackerel, 21.5% in small size of horse mackerel, 6.7% in black sea-bream, 17.8% in red barracuda, 16.8 in half-beak, 19.1% in gizzard shad individually, and totally in six species of fish, that was 18.8%. 3. The leading ratio from side points of the leader departed from door in 25m to fish court was 58.9% in small size of mackerel, 74.6% in small size of horse mackerel, 38.0% in black sea-bream, 54.7% in red barracuda, 58.6% in half-beak, 54.5% in gizzard shad individually, and totally in six species of fish, that was 57.8%. So it was assumed that the leader of set-net was very effective in leading to the swimming direction of small size of mackerel, small size of horse mackerel, red barracuda, half-beak and gizzard shad. 4. Red barracuda, half-beak and gizzard shad entered into bag net of upstream in large numbers than bag net of downstream, and small size of horse mackerel and black sea-bream entered into bag net of downstream in large numbers than bag net of upstream. 5. Small size of mackerel and small size of horse mackerel had high remaining rate in the bag net of downstream, and black sea-bream, red barracuda and half-beak had high remaining rate in the bag net of upstream.

• Journal of fish pathology
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• v.26 no.2
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• pp.99-110
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• 2013

Hatchery-reared seeds provides a key source of animal protein for human consumption and restocking for fishery management. For stock enhancement program, we have inspected the hatchery-reared seeds of 33 species in 2009, 44 species in 2010, 43 species in 2011 and 46 species in 2012 for legally designated diseases. Results showed that abalone was the most abundant in the marine species group and then sea cucumber, olive flounder, rockfish and swimming crab were followed. Crucian carp was the most abundant and then mandarin fish, Korean bullhead, melanian snail and Chinese mitten crab were followed in the freshwater species group. The number of inspection for black sea bream, rock bream, scorpionfish, black scraper, and eel has continuously decreased for four years. The inspection for flathead mullet has been carried out only in 2009. The total number of inspection cases for eight pathogens in this study were 8,476 and disqualification cases were 56 (0.67%) by detection of aquatic animals pathogens such as koi herpesvirus, white spot syndrome virus, red sea bream iridovirus or viral haemorrhagic septicemia virus.

• Journal of fish pathology
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• v.23 no.1
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• pp.85-98
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• 2010

Ministry of Agriculture, Fisheries and Food (MAFF) and National Fisheries Research and Development Institute (NFRDI) have inspected the hatchery-reared seeds of 22 marine species and 11 freshwater species for aquatic animal diseases in stock enhancement program in 2009. Results showed that total 12 local selfgovernments have been restocking the sea with cultured juveniles. Gyeongsangnam-do, Jeollanam-do, Jejudo and Chungcheongnam-do have a preference for marine species seeds to freshwater species. On the contrary, freshwater species were released mostly in Gyeonggi-do, Jeollabuk-do and Chungcheongbuk-do. In the marine species group, abalone was the most abundant as 24.5%, and then sea cucumber (15.2%), olive flounder (11.5%), swimming crab (5.6%), black sea bream and rockfish (6.8%), rock bream (5.1%), black rockfish (4.6%) and scorpionfish (4.5%) were followed. Crucian carp was the most abundant as 19.4%, and then eel (17.0%), Korean bullhead (12.3%), melanian snail (12.0%), catfish (8.4%) were followed in the freshwater species group. The total number of inspection cases in this study were 1,080 and disqualification cases were 19 by detection of aquatic animals pathogens such as red sea bream iridovirus (RSIV), koi herpesvirus (KHV) or white spot syndrome virus (WSSV).

• Journal of fish pathology
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• v.1 no.1
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• pp.39-43
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• 1988

Three strains of pathogenic bacteria isolated from diseased red sea bream at a culture farm in Gyoung sangnam-Do during the midwinter of 1987 were studied taxonomically. Based on their biological and biochemical characteristics, they were identified as Vibrio sp.. They exhibited very similarities to group II (Kusuda et al.,1979) except that the former can grow at lower temperature.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.3
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• pp.241-246
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• 2014

Viruses in the genus Megalocytivirus have been subdivided into four subgroups. Among these subgroups 2 and 4, represented by the red sea bream iridovirus (RBIV) and the olive flounder iridovirus (FLIV), respectively, are non-exotic. subgroups 1 and 3, represented by the red sea bream iridovirus (RSIV) and the infectious spleen and kidney necrosis virus (ISKNV), respectively, have not been detected in Korea and are known as exotic. Shellfish are filter-feeders, and can thus filter and accumulate Megalocytivirus in their digestive glands, allowing us to track viral contamination in surrounding aquatic environment. In this study, we developed a high-resolution melting (HRM) analysis to differentiate among subgroups of Megalocytivirus accumulated in shellfish, and confirmed the convenience and efficiency of this method. More than two subgroups of Megalocytivirus were found in the digestive gland of a single shellfish. We classified all Megalocytivirus viruses from shellfish in Korea into subgroups 2 and 4, although proportions of subgroups were different among regions. Compared to nucleotide sequencing analysis, HRM analysis is a simple and rapid method for differentiating of Megalocytivirus subgroups.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.49 no.3
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• pp.227-237
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• 2013

In Jeju Island, red sea bream is mainly caught by long line with a live squid as bait. The movements of artificial jigs in the fishery are supposed to be an important factor for catching the target organism as well as bait. To develop new fishing method for long line fishery, movements of the bait which was squid were tried to be characterized. In a water tank experiment, a live squid was hooked by a fish hook attached to a model long line. And then movements of squids in the water tank were recorded for 20 minutes by a video camera. Recorded movement of the squid was just periodically up-and-down moving, with a dominant frequency of 0.125Hz, and amplitude of 10.8cm.