Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Correlation between clinical changes and viral genome copy number in rock bream infected with red sea bream iridovirus

참돔이리도바이러스 감염 돌돔에서 임상적 변화와 viral genome copy number 간의 상관관계

  • Dong Jun Shin (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Yi Seol Jeong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Min Jae Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Guk Hyun Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kwang Il Kim (Department of Aquatic Life Medicine, Pukyong National University)
  • 신동준 (부경대학교 수산생명의학과) ;
  • 정이설 (부경대학교 수산생명의학과) ;
  • 김민재 (부경대학교 수산생명의학과) ;
  • 김국현 (부경대학교 수산생명의학과) ;
  • 김광일 (부경대학교 수산생명의학과)
  • Received : 2023.11.06
  • Accepted : 2023.11.27
  • Published : 2023.12.31
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Abstract

In this study, the correlation between clinical changes and RSIV genome copy number was investigated to determine the quantitative criteria for the characteristics of RSIV infection. The rock bream (Oplegnathus fasciatus) was intraperitoneally injected with three different doses (1.0×101, 1.0×103 and 1.0×105 viral genome copies/fish) as low, medium, and high doses, respectively. The clinical signs (spleen enlargement, death) observation and real-time PCR were conducted at 5, 10 and 14 days post-injection. During the experiment, spleen index as a quantitative indicator for spleen enlargement was continuously increased in the medium- (up to 2.26) and high-dose (up to 4.99) challenge groups, respectively. Notably, when the spleen index was over 1.5, 2.0, 2.5 and 3.0, a positive correlation was revealed with average viral genome copy numbers of 2.51, 3.37, 4.97 and 5.43×107 viral genome copies/mg, respectively. Moreover, the threshold of spleen index over 1.5 was 1.0×106 viral genome copies/mg, while the thresholds of spleen index over 2.0 and dead was 2.51×107 viral genome copies/mg and the thresholds of spleen index over 2.5 and 3 was 3.98×107 viral genome copies/mg. These findings suggest the possibility of quantitatively analyzing the characteristics and development process of RSIV infection.

Keywords

Acknowledgement

이 논문은 2022년도 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업임(No. 2022R1I1A2064371)

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