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Red and Blue Photons Can Enhance the Production of Astaxanthin from Haematococcus pluviatis

  • Kim, Z-Hun;Lee, Ho-Sang;Lee, Choul-Gyun
    • ALGAE
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    • v.24 no.2
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    • pp.121-127
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    • 2009
  • The unicellular green alga, Haematococcus pluvialis, accumulates the highest level of astaxanthin among knownastaxanthi.n-producing organisms. Light is the most important factor to induce astaxanthin by H. pluvialis. BIue andred LEDs, whose ${\lambda}_{max}$'s are 470 and 665 nm, respectively, were used for internally illuminated light sources.Fluorescent lamps were also used for both internal and external illumination sources. The astaxanthin levels in thesevarious lighting systems were analyzed and compared each other. The cultures under internally illuminated LEDsaccumulaled 20% more astaxanthin than those under fluorescent lamp. Furthermore, LEDs generated much lessheat than the fluorescent lamps, which gives one more reason for the LEDs being a suitable internal Light source forastaxanthin induction. The results reported here would lead novel designs of photobioreactors with improvementsof illumination methods for high level of astaxanthm production. The maximum astaxanthin concentrations as wellas the astaxanthin yield per supplied photon were increased by at least 20% when blue or red LEDs were supplied.

Effect of LED Light Quality and Intensity on Growth Characteristics of Ginseng Cultivated in Plastic House

  • Sang Young Seo;Jong hyeon Cho;Chang Su Kim;Hyo Jin Kim;Min Sil An;Du Hyeon Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.61-61
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    • 2020
  • This experiment was carried out using artificial bed soil and LED in the plastic film house(irradiation time: 07:00-17:00/day). Seedlings(n=63 per 3.3 m2) of ginseng was planted on May 17, 2018. LED was combined with red and blue light in a 3:1 ratio and irradiated with different light intensity(40-160 µmol/m2/s). Average air temperature from April to September according to the light intensity test was 20.4℃-20.9℃. Average artificial bed soil temperature was 20.1℃-21.7℃. The test area where fluorescent lamp was irradiated tended to be somewhat lower than the LED irradiation area. The chemical properties of the test soil was as follows. pH levels was 6.6-6.7, EC levels 0.9-1.3 dS/m and OM levels 30.6-32.0%. The available P2O5 contents was 73.3-302.3 mg/kg. Exchangeable cations K and Ca contents were higher than the allowable ranges and mg content was high in the fluorescent lamp treatment. The photometric characteristics of LED light intensity are as follows. The greater the light intensity, the higher the PPFD(Photosynthetic Photon Flux Density) value, illuminance and solar irradiation. Fluorescent lamp treatment had high illuminance value, but PPFD and solar irradiation were lower than LED intensity 40 µmol/m2/s treatment. The photosynthetic rate increased(2.0-3.8 µmolCO2/m2/s) as the amount of light intensity increased, peaking at 120 µmol/m2/s, and then decreasing. The SPAD (chlorophyll content) value decreased as the amount of light intensity increased, and was the highest at 36.1 in fluorescent lamp treatment. Ginseng germination started on April 5 and took 14-17 days to germinate. The overall germination rate was 68.8-73.6%. The growth of aerial parts(plant height etc.) were generally excellent in the treatment of light intensity of 120-160 µmol/m2/s. The plant height was 41.9 cm, stem length was 24.1 cm, leaf length was 9.8 cm and stem diameter was 5.6 mm. The growth of underground part (root length etc.) was the best in the treatment with 120 µmol/m2/s of light intensity. Due to the root length was long(24.8 cm) and diameter of taproot was thick(18.7 mm), the fresh root weight was the heaviest at 24.8 g. There were no disease incidence such as Alternaria blight, Gray mold and Anthracnose. Disease of Damping-off caused by Rhizoctonia solani occurred 0.6-1.5% and incidence ratio of rusty root ginseng was 30.8-62.3%. It is believed that the reason for the high incidence of rusty root ginseng is that the amount of field moisture capacity of artificial bed soil is larger than the soil. Leaf discoloration rate was 13.7-32.3%.

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Effect of different light sources and ventilation on in vitro shoot growth and rooting of a rare and endangered species, Tsuru-rindo(Tripterospermum japonicum) (희귀 및 멸종위기 식물 덩굴용담의 기내생장에 미치는 광질 및 환기효과)

  • Moon, Heung-Kyu;Park, So-Young
    • Journal of Plant Biotechnology
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    • v.35 no.3
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    • pp.215-221
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    • 2008
  • Effects of light generated by LEDs on shoot growth and rooting of Tsuru-rindo(Tripterospermum japonicum) were evaluated. Apical shoots(one or two node with 3-4 leaves) were cultured on MS basal medium with 3% sucrose and maintained for four weeks under five different light qualities: fluorescent lamp(F), 100% red LED(R), 70% red LED+30% blue LED(R7B3), 50% red LED+50% blue(R5B5), or 100% blue LED(B). Rooting was promoted by both red light and fluorescent lamp, and the effect was further promoted under the ventilation. Red light enhanced shoot node elongation, whereas blue light appeared to suppress it. Growth of shoots and leaves were enhanced under the ventilation irrespective of the different light qualities. Under the ventilated condition, total fresh weight of plants was highest in R7B3 LED as 257.7 mg per plant. Dry matters, which are used for index of plant growth, were lowest under red light, whereas it was highest under blue light. The dry matter was inclined to getting higher by ascending the ratio of blue light and red light. Total chlorophyll content was highest in both R7B3 LED and R5B5 LED under ventilation as 29.5 and 31.2, respectively. Above results suggest that light quality optimization could be an important factor to foster in vitro growth of the species. Ventilation treatment appeared to be another important factor to induce normal shoot growth and rooting.

Effects of Light-emitting Diodes on In Vitro Growth of Virus-free Sweet Potato Plantlets (LED가 고구마 바이러스 무병묘의 기내 생장에 미치는 영향)

  • Yoo, Kyoung-Ran;Lee, Seung-Yeob
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.490-498
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    • 2017
  • The in vitro growth of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets was investigated under different light sources: fluorescent lamp (control); red (660 nm), blue (460 nm), white light-emitting diodes (LED), and two mixtures of blue and red LED (R:B = 8:2, and 7:3). Single node explants (10 mm) of three cultivars ('Matnami', 'Shincheonmi', and 'Yeonhwangmi') were cultured on Murashige and Skoog medium supplemented with $0.2mg{\cdot}L^{-1}$ 6-benzyladenine for 4 weeks. Explants were exposed to $150{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux at a distance of 20 cm, constant temperature of $25^{\circ}C$, and under 16/8-h (day/night) photoperiod. Using the same method, the in vitro growth of 10 cultivars under red LED was also compared. After 3 weeks, vine length was highest in plantlets cultured under red LED, and lowest in plantlets cultured under blue LED. Fresh and dry weights were also greatest in plantlets cultured under red LED. Compared to the control, vine thickness was significantly higher in plantlets grown under white LED and the 7:3 R:B LED mixture. Significant differences were observed among the 10 cultivars grown under red LED. 'Matnami', 'Shincheonmi', and 'Shinhwangmi' all had excellent vine lengths, and fresh and dry weights. Compared to the control, vine elongation of sweet potato plantlets was most effective under red LED, and culture duration was about 1 week shorter.

Single crystal growth and characterization of changeable colored cubic zirconia (변색효과 cubic zirconia의 단결정 성장과 특성평가)

  • Park, Byeong-Seok;Choi, Jong-Keon
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.17 no.3
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    • pp.108-112
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    • 2007
  • Cubic Zirconia single crystals having changeable color with light source were grown by skull melting method. Strong absorption of yellow color region light by Co and Nd and the absorption in violet color region by Fe led the color change from blue-green to red-purple with the light source from fluorescent to incandescent lamp. Color of crystals varied not only by the dopants but yttria contents and the conditions far heat treatment.

Screening of Seaweed Extracts for Algicidal Substances Using a Photosensitization Effect (해조류 추출물로부터의 Photosensitizing 효과를 지니는 항 적조물질 탐색)

  • Jin, Hyung-Joo;Jin, Deuk-Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.3
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    • pp.122-127
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    • 2007
  • Photosensitized extracts of 28 seaweed species were tested in vitro to examine the variation in their algicidal activities against the toxic microalga Cochlodinium polykrikoides. When both seaweed extracts and microalgae were exposed to UV and visible light, methanol extracts of Porphyra yezoensis showed 5.7- and 4.4-fold increasesin light-dependent algicidal activity, respectively. When only seaweed extracts were exposed to both UV (365 nm) and visible light (white lamp) at the same time, methanol extracts of Enteromorpha linza and Carpopeltis affinis showed 3.3- and 3.4-fold increases in algicidal activity, respectively. When UV-photosensitized extracts were left in the dark, the algicidal activity of Ecklonia cava increased 13-fold after 5 h. When visible light-photosensitized extracts were left in the dark, the algicidal activity of Monostroma nitidum increased by 3.3-fold in 1 h.

LED신호등의 원형 배열을 이용한 새로운 신호 체계 설계구현

  • Park, Yong-Man;Kwon, Jong-Won;Odgere, Odgere;Kim, Hie-Sik
    • Proceedings of the KIEE Conference
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    • 2006.04a
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    • pp.348-350
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    • 2006
  • Currently the indication method of the traffic signal light which is established in our country displays each "red, yellow, green" using the brightness LED signal light. In this paper, one LED circular arrangement replaces the Current indication method of the traffic signal light using two color LED. Also the blinking signal function of present time uses the MCU of AVR series and it is embodied with magnification and abridgment of the circular arrangement for recognizing easily far away. The signal light indication method which is proposed will be able to substitute the signal light of 3 colors with 1 signal light with economic profit presented the reform measure of energy efficiency improvement together.

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The Soft Material Obtained from an Europium (III)-Containing Ionic Liquid

  • Shao, Huifang;Wang, Yige;Li, Dan
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.973-976
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    • 2011
  • The addition of organic ligand (${\beta}$-diketone or heterocyclic compound) to the europium (III)-containing ionic liquid resulted in bright luminescent soft materials with the molar ratio of europium/ionic liquid (IL)/ligand being 1:3:1 that exhibit bright red light under UV lamp. The luminescent properties such as emission features and lifetime of $^5D_0$ $Eu^{3+}$ excited level are dependent on the organic ligands. The materials were characterized by FT-IR and luminescence spectroscopy. The data shows that at least parts of the ILs (carboxylic acid) are replaced with ${\beta}$-diketone ligand rather than the formation of europium complex with the molar ratio of $Eu^{3+}$:IL: ligand being 1:3:1, while no ILs could be replaced by the heterocyclic ligand such as Bpy and Phen.

Leaf Shape Index, Growth, and Phytochemicals in Two Leaf Lettuce Cultivars Grown under Monochromatic Light-emitting Diodes (단색 발광다이오드에서 자란 축면상추 두 품종의 엽형, 생장 및 기능성 물질)

  • Son, Ki-Ho;Park, Jun-Hyung;Kim, Daeil;Oh, Myung-Min
    • Horticultural Science & Technology
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    • v.30 no.6
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    • pp.664-672
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    • 2012
  • As an artificial light source, light-emitting diode (LED) with a short wavelength range can be used in closed-type plant production systems. Among various wavelength ranges in visible light, individual light spectra induce distinguishing influences on plant growth and development. In this study, we determined the effects of monochromatic LEDs on leaf shape index, growth and the accumulation of phytochemicals in a red leaf lettuce (Lactuca sativa L. 'Sunmang') and a green leaf lettuce (Lactuca sativa L. 'Grand rapid TBR'). Lettuce seedlings grown under normal growing conditions ($20^{\circ}C$, fluorescent lamp + high pressure sodium lamp, $130{\pm}5{\mu}mmol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 hours photoperiod) for 18 days were transferred into incubators at $20^{\circ}C$ equipped with various monochromatic LEDs (blue LED, 456 nm; green LED, 518 nm; red LED, 654 nm; white LED, 456 nm + 558 nm) under the same light intensity and photoperiod ($130{\pm}7{\mu}mmol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 hours photoperiod). Leaf length, leaf width, leaf area, fresh and dry weights of shoots and roots, shoot/root ratio, SPAD value, total phenolic concentration, antioxidant capacity, and the expression of a key gene involved in the biosynthesis of phenolic compounds, phenylalanine ammonia-lyase (PAL), were measured at 9 and 23 days after transplanting. The leaf shape indexes of both lettuce cultivars subjected to blue or white LEDs were similar with those of control during whole growth stage. However, red and green LEDs induced significantly higher leaf shape index than the other treatments. The green LED had a negative impact on the lettuce growth. Most of growth characteristics such as fresh and dry weights of shoots and leaf area were the highest in both cultivars subjected to red LED treatment. In case of red leaf lettuce plants, shoot fresh weight under red LED was 3.8 times higher than that under green LED at 23 days after transplanting. In contrast, the accumulation of chlorophyll, phenolics including antioxidants in lettuce plants showed an opposite trend compared with growth. SPAD value, total phenolic concentration, and antioxidant capacity of lettuce grown under blue LED were significantly higher than those under other LED treatments. In addition, PAL gene was remarkably activated by blue LED at 9 days after transplanting. Thus, this study suggested that the light quality using LEDs is a crucial factor for morphology, growth, and phytochemicals of two lettuce cultivars.