Browse > Article
http://dx.doi.org/10.5010/JPB.2008.35.3.215

Effect of different light sources and ventilation on in vitro shoot growth and rooting of a rare and endangered species, Tsuru-rindo(Tripterospermum japonicum)  

Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute(KFRI))
Park, So-Young (Division of Biotechnology, Korea Forest Research Institute(KFRI))
Publication Information
Journal of Plant Biotechnology / v.35, no.3, 2008 , pp. 215-221 More about this Journal
Abstract
Effects of light generated by LEDs on shoot growth and rooting of Tsuru-rindo(Tripterospermum japonicum) were evaluated. Apical shoots(one or two node with 3-4 leaves) were cultured on MS basal medium with 3% sucrose and maintained for four weeks under five different light qualities: fluorescent lamp(F), 100% red LED(R), 70% red LED+30% blue LED(R7B3), 50% red LED+50% blue(R5B5), or 100% blue LED(B). Rooting was promoted by both red light and fluorescent lamp, and the effect was further promoted under the ventilation. Red light enhanced shoot node elongation, whereas blue light appeared to suppress it. Growth of shoots and leaves were enhanced under the ventilation irrespective of the different light qualities. Under the ventilated condition, total fresh weight of plants was highest in R7B3 LED as 257.7 mg per plant. Dry matters, which are used for index of plant growth, were lowest under red light, whereas it was highest under blue light. The dry matter was inclined to getting higher by ascending the ratio of blue light and red light. Total chlorophyll content was highest in both R7B3 LED and R5B5 LED under ventilation as 29.5 and 31.2, respectively. Above results suggest that light quality optimization could be an important factor to foster in vitro growth of the species. Ventilation treatment appeared to be another important factor to induce normal shoot growth and rooting.
Keywords
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Sanjaya, Muthan B, Rathore TS, Rai VR (2006) Micropropagation of an endangered Indian sandalwood (Santalum album L.). J For Res 11: 203-209   DOI
2 Shin KS, Murthy HN, Heo JW, Hahn EJ, Paek KY (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plant 30: 339-343   DOI
3 Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoides, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144: 307-313   DOI   ScienceOn
4 Zobayed SMA (2005) Ventilation in micropropagation. T. Kozai et al. (eds). Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system, Springer, pp 147-186
5 Kim JA, Moon HK (2006) Effect of light-emitting diodes (LEDs) and ventilation on the in vitro shoot growth of Eucalyptus pellita. J Korean For Soc 95: 716-722
6 Heo JW, Lee CW, Chakrabarty D, Paek KY (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Reg. 38: 225-230   DOI   ScienceOn
7 Gonzlez A, Arigita L, Majada J, Snchez Tams R (1997) Ethylene involvement in in vitro organogenesis and plant growth of Populus tremula L. Planr Growth Regul. 22: 1-6   DOI   ScienceOn
8 Bula RJ, Morrow TW, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS (1991) Light-emitting diodes as a radiation sources for plants. HortSci 26: 203-205
9 Dooley JH (1991) Influence of lighting spectra on plant tissue culture. ASAE (American Society of Agricultural Engineers) Meeting, Chicago, Paper No. 917530
10 Bang JW, Lee MK, Chung SH (1994) Somatic embryogenesis and plant regeneration in leaf explant cultures of Gentiana scabra var. buergeri. Korean J Plant Tiss Cult 21: 233-237
11 Seong NS, Park CH, Kim KS, Lee ST, Chang YH (1996) In vitro variant induction and its content of gentiopicroside of Gentiana scabra Bunge. Korean J Medicinal Crop Sci. 3: 40-44
12 Sung IK, Kitoya M, Hirano T (1998) The effects of time and intensity of supplemental blue lighting during morning twilight on growth and physiological performance of cucumber seedlings. Life Support and Biosphere Sci 5: 137-141
13 SAS (1989) SAS/STAT User's Guide, Version 6, Ed 4, Vol2. SAS Institute, Cary
14 Seabrook JEA (2005) Light effects on the growth and morphogenesis of potato (Solanum tuberosum) in vitro: a review. Amer J Potato Res 82: 353-367   DOI
15 Lim JD, Yu CY (2000) Multiple shoot formation of Gentiana axillariflora leveille by in vitro culture. Korean J Medicinal Crop Sci 8: 41-48
16 Mcree KJ (1972) The action spectra, absorptance and quantum yield of photosynthesis in crop plants. J Agricult Metro 9: 191-196
17 Lee YM, Lee WR (1997) Illustrated rare and endangered species in Korea. Korea Forest Research Institute, Jungbu Forest Experimental Station, pp 255
18 Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hort 101: 143-151   DOI   ScienceOn
19 Nguyen QT, Kozai T (2005) Photoautotrophic micropropagation of woody species. In: T Kozai et al. (eds), Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system, pp 123-146
20 Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Response of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tiss Org Cult 73: 43-52   DOI   ScienceOn
21 Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant. Plant Biotechnol Rep 1: 155-161   DOI
22 Nhut DT, Don NT, Tanaka M (2007) Light-emitting diodes as an effective lighting sources for in vitro banana culture. In: S.M. Jain and Haggman (eds), Protocols for Micropropagation of Woody Trees and Fruits, Springer press, pp 527-541
23 Poudel PR, Kataoka I, Mochioka R (2008) Effect of red and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Org Cult 92: 147-153   DOI
24 Seong NS, Park CH, Lee ST, Kim SM (1993) Plant regeneration and multiplication of Gentiana scabra bunge through leaf and stem culture. Korean J Medicinal Crop Sci 1: 129-136
25 Kartsonas E, Papafotiou M (2007) Mother plant age and seasonal influence on in vitro propagation of Quercus euboica Pap., an endemic, rare and endangered oak species of Greece. Plant Cell Tiss Org Cult 90: 111-116   DOI
26 Moon HK, Park SY, Kim YW and Kim CS (2006) Growth of Tsuru-rindo (Tripterospermum japonicum) cultured in vitro under various sources of light-emitting diode (LED) irradiation. J Plant Biol 49: 174-179   과학기술학회마을   DOI   ScienceOn
27 Muleo R., Morini S, Casano S (2001) Photoregulation of growth and branching of plum shoots: physiological action of two photosystems. In Vitro Cell Dev Biol-Plant 37: 609-617   DOI
28 Muleo R, Morini S (2006) Light quality regulates shoot cluster growth and development of MM 106 apple genotype in in vitro culture. Sci Hort 108(4): 364-370   DOI   ScienceOn
29 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473-479   DOI