• Title/Summary/Keyword: real-time process

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Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs

  • Chen Chen;Yitong Chang;Yuan Deng;Qingming Cui;Yingying Liu;Huali Li;Huibo Ren;Ji Zhu;Qi Liu;Yinglin Peng
    • Animal Bioscience
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    • v.36 no.3
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    • pp.404-416
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    • 2023
  • Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

Temperature Compensation on the Cone Tip Resistance by Using FBG Temperature Transducer (FBG센서를 이용한 콘 선단저항력의 온도영향 보상)

  • Kim, Rae-Hyun;Lee, Jong-Sub;An, Shin-Whan;Lee, Woo-Jin
    • Journal of the Korean Geotechnical Society
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    • v.25 no.10
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    • pp.31-40
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    • 2009
  • As the measurement of strain-gage type cone penetrometer is influenced by the temperature change during penetration, the temperature is a factor producing an error of the cone tip resistance. In this study, the 0.5 mm diameter temperature transducer and 7 mm diameter micro cone penetrometer are manufactured by using FBG sensors to evaluate the effect of temperature on the cone tip resistance. Design concepts include the cone configuration, sensor installation and the temperature compensation process. The test shows that the tip resistance measured by strain gauge is affected by the temperature change. The error of the tip resistance increases with an increase in temperature change, while the temperature effect on the tip resistance of FBG cone is effectively compensated by using FBG temperature transducer. Temperature compensated tip resistance of the strain gauge cone shows the good matched profile with FBG cone which performs real-time temperature compensation during penetration. This study demonstrates that the temperature compensation by using FBG sensor is an effective method to produce the more reliable cone tip resistance.

An Automatic ROI Extraction and Its Mask Generation based on Wavelet of Low DOF Image (피사계 심도가 낮은 이미지에서 웨이블릿 기반의 자동 ROI 추출 및 마스크 생성)

  • Park, Sun-Hwa;Seo, Yeong-Geon;Lee, Bu-Kweon;Kang, Ki-Jun;Kim, Ho-Yong;Kim, Hyung-Jun;Kim, Sang-Bok
    • Journal of the Korea Society of Computer and Information
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    • v.14 no.3
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    • pp.93-101
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    • 2009
  • This paper suggests a new algorithm automatically searching for Region-of-Interest(ROI) with high speed, using the edge information of high frequency subband transformed with wavelet. The proposed method executes a searching algorithm of 4-direction object boundary by the unit of block using the edge information, and detects ROIs. The whole image is splitted by $64{\times}64$ or $32{\times}32$ sized blocks and the blocks can be ROI block or background block according to taking the edges or not. The 4-directions searche the image from the outside to the center and the algorithm uses a feature that the low-DOF image has some edges as one goes to center. After searching all the edges, the method regards the inner blocks of the edges as ROI, and makes the ROI masks and sends them to server. This is one of the dynamic ROI method. The existing methods have had some problems of complicated filtering and region merge, but this method improved considerably the problems. Also, it was possible to apply to an application requiring real-time processing caused by the process of the unit of block.

Development of Deep Learning Model for Detecting Road Cracks Based on Drone Image Data (드론 촬영 이미지 데이터를 기반으로 한 도로 균열 탐지 딥러닝 모델 개발)

  • Young-Ju Kwon;Sung-ho Mun
    • Land and Housing Review
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    • v.14 no.2
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    • pp.125-135
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    • 2023
  • Drones are used in various fields, including land survey, transportation, forestry/agriculture, marine, environment, disaster prevention, water resources, cultural assets, and construction, as their industrial importance and market size have increased. In this study, image data for deep learning was collected using a mavic3 drone capturing images at a shooting altitude was 20 m with ×7 magnification. Swin Transformer and UperNet were employed as the backbone and architecture of the deep learning model. About 800 sheets of labeled data were augmented to increase the amount of data. The learning process encompassed three rounds. The Cross-Entropy loss function was used in the first and second learning; the Tversky loss function was used in the third learning. In the future, when the crack detection model is advanced through convergence with the Internet of Things (IoT) through additional research, it will be possible to detect patching or potholes. In addition, it is expected that real-time detection tasks of drones can quickly secure the detection of pavement maintenance sections.

Proposals on Basic Data Based on Comparison of Changes in Clinical Laboratory Technologists' National Examination and Job Definition: Focused on Korea, Japan, and Taiwan (임상병리사국가시험 및 직무의 변천 비교를 중심으로 한 기초자료 제안: 한국, 일본, 대만을 중심으로)

  • Bon-Kyeong KOO;Myung Soo KIM;Yoon Sik KIM;Jun Ho LEE
    • Korean Journal of Clinical Laboratory Science
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    • v.55 no.2
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    • pp.71-81
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    • 2023
  • This study examined the transition process of clinical laboratory technologists' national examination and job definition in Korea and compared the differences in the national examination between Korea and neighboring countries, such as Japan and Taiwan. In Korea, the number of questions made for it was 200 (1965), 200 (1977), 300 (1982), 250 (1992), 330 (2006), and 280 (2015). The practice of clinical physiology is important for real-time monitoring, given the characteristics of physiological testing. On the other hand, there are conflicts between other occupations in the working area. Clinical molecular biology needs to be established as a new major subject considering the diagnostic importance of molecular biological tests and the speed of science and technology development. Clinical laboratory operations provide policy and guidance recommendations to technologist staff. The proposed clinical laboratory technologists' national examination comprises major subjects: clinical biochemistry, clinical hematology, clinical transfusionology, clinical immunology, clinical microbiology, clinical molecular biology, clinical histology, clinical cytology, clinical physiology, and clinical laboratory operations. In addition, this study proposes the job definition of clinical laboratory technologists, revising various chemical or physiological testing to biomedical or physiological testing required for medical practice.

The study of security management for application of blockchain technology in the Internet of Things environment (Focusing on security cases in autonomous vehicles including driving environment sensing data and occupant data) (사물인터넷 환경에서 블록체인 기술을 이용한 보안 관리에 관한 소고(주행 환경 센싱 데이터 및 탑승자 데이터를 포함한 자율주행차량에서의 보안 사례를 중심으로))

  • Jang Mook KANG
    • Convergence Security Journal
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    • v.22 no.4
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    • pp.161-168
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    • 2022
  • After the corona virus, as non-face-to-face services are activated, domain services that guarantee integrity by embedding sensing information of the Internet of Things (IoT) with block chain technology are expanding. For example, in areas such as safety and security using CCTV, a process is required to safely update firmware in real time and to confirm that there is no malicious intrusion. In the existing safe security processing procedures, in many cases, the person in charge performing official duties carried a USB device and directly updated the firmware. However, when private blockchain technology such as Hyperledger is used, the convenience and work efficiency of the Internet of Things environment can be expected to increase. This article describes scenarios in how to prevent vulnerabilities in the operating environment of various customers such as firmware updates and device changes in a non-face-to-face environment. In particular, we introduced the optimal blockchain technique for the Internet of Things (IoT), which is easily exposed to malicious security risks such as hacking and information leakage. In this article, we tried to present the necessity and implications of security management that guarantees integrity through operation applying block chain technology in the increasingly expanding Internet of Things environment. If this is used, it is expected to gain insight into how to apply the blockchain technique to guidelines for strengthening the security of the IoT environment in the future.

A Study on the Development of Traffic Accident Information System Based on WebGIS (WebGIS 기반 교통사고정보관리 시스템 개발에 관한 연구)

  • Jeong, Su-Jin;Lim, Seung-Hyeon;Cho, Gi-Sung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.6D
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    • pp.1003-1010
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    • 2006
  • This study developed a traffic accident information management system based on WebGIS that can process a lot of data for giving effectively diagnosis of traffic accidents in serious damage circumstances by traffic accident. Also, this study presents a way to compose and to convey traffic accident information. In addition, non-spatial attributes as well as spatial attributes about traffic accidents information be integrated and managed by the system. To provide Web service, we developed modules that can supply visually spatial information and traffic accidents data through ASP, Javascript, ArcIMS based on Web and constructed a server. And constructed system include a function that offer the now situation of traffic accident in real time, which supply the statistical data of traffic accident through Web as soon as user entry data in comparison with previous way that preparatory period until traffic accidents data is supplied to peoples had been long. Traffic accidents are analyzed with only nonspatial attribute by simply collecting in the past. However, system constructed by this study offer new function that can grasp visually accident spot circumstance and use detailed content and accurate location data as well as statistical data of traffic accidents. Also, it offer interface that can connect directly with accident charge policeman.

Identification and functional prediction of long non-coding RNAs related to oxidative stress in the jejunum of piglets

  • Jinbao Li;Jianmin Zhang;Xinlin Jin;Shiyin Li;Yingbin Du;Yongqing Zeng;Jin Wang;Wei Chen
    • Animal Bioscience
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    • v.37 no.2
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    • pp.193-202
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    • 2024
  • Objective: Oxidative stress (OS) is a pathological process arising from the excessive production of free radicals in the body. It has the potential to alter animal gene expression and cause damage to the jejunum. However, there have been few reports of changes in the expression of long noncoding RNAs (lncRNAs) in the jejunum in piglets under OS. The purpose of this research was to examine how lncRNAs in piglet jejunum change under OS. Methods: The abdominal cavities of piglets were injected with diquat (DQ) to produce OS. Raw reads were downloaded from the SRA database. RNA-seq was utilized to study the expression of lncRNAs in piglets under OS. Additionally, six randomly selected lncRNAs were verified using quantitative real-time polymerase chain reaction (qRT-PCR) to examine the mechanism of oxidative damage. Results: A total of 79 lncRNAs were differentially expressed (DE) in the treatment group compared to the negative control group. The target genes of DE lncRNAs were enriched in gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) signaling pathways. Chemical carcinogenesis-reactive oxygen species, the Foxo signaling pathway, colorectal cancer, and the AMPK signaling pathway were all linked to OS. Conclusion: Our results demonstrated that DQ-induced OS causes differential expression of lncRNAs, laying the groundwork for future research into the processes involved in the jejunum's response to OS.

Proposals for Revising the Occupational Exposure Limits for Aluminum in Korea (국내 알루미늄 노출실태 및 노출기준 개정 제안)

  • Seung Won Kim;Young Gyu Phee;Yong-Joon Baek;Taejin Chung;Hye-Sil Lee
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.34 no.1
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    • pp.85-97
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    • 2024
  • Objectives: This study was intended to investigate the revision status of the occupational exposure standards for aluminum at home and abroad; to investigate worker exposure at domestic aluminum manufacturing and handling workplaces; to conduct social and economic evaluation for the revision of domestic aluminum exposure limits. Methods: We investigated the current status of occupational exposure limits for aluminum at home and abroad, and analyzed supporting data. An exposure survey was conducted targeting domestic aluminum manufacturing and handling workplaces. Based on these, revised aluminum occupational exposure limits were proposed. Results: The major aluminum exposure limits at home and abroad show a notable difference. The toxicity of aluminum, which was revealed through animal experiments and epidemiological investigations. The average concentration of aluminum in the air at 12 workplaces was 0.016 mg/m3, and the maximum was 0.0776 mg/m3. When total dust and respiratory dust were measured side by side and simultaneously for the same process, 12.1% of the total mass concentration of aluminum dust was respiratory dust. As a result of measuring and comparing the size distribution of dust with an optical particle counter in real time, 48.1% of the total dust in the form of welding fume and pyro-powder was respiratory dust. Based on the literature review and workplace survey, three proposals for changing the aluminum exposure limit were proposed. Proposal (1): For all types, 10 mg/m3 is unified as the exposure limit except for soluble salts and alkyls. Proposal (2): 1(R) mg/m3 as the exposure limit for all forms except soluble salts and alkyl. Proposal (3): 1(R) mg/m3 for pyro-powder and welding fume, and 10 mg/m3 for metal dust, aluminum oxide, and insoluble compounds as exposure standards. A pyro-powder was defined as dry aluminum powder of 200 mesh size (74 ㎛) or smaller (larger size classified as metal dust). Reason for setting: In the workplace survey, the ratio of respiratory dust to total dust was analyzed to be about 1:10, so it was judged that the domestic standard and the ACGIH standard were compatible. Conclusions: In all scenarios according to the revision of the exposure standard, the B/C ratio was greater than 1 or only benefits existed, so it was evaluated as sufficiently reasonable as a result of the socio-economic evaluation.

Integrated Sensing Module for Environmental Information Acquisition on Construction Site (건설현장 환경정보 수집을 위한 통합 센싱모듈 개발)

  • Moon, Seonghyeon;Lee, Gitaek;Hwang, Jaehyun;Chi, Seokho;Won, Daeyoun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.44 no.1
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    • pp.85-93
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    • 2024
  • The monitoring of environmental information (e.g. noise, dust, vibration, temperature, humidity) is crucial to the safe and sustainable operation of a construction site. However, commercial sensors exhibit certain drawbacks when applied on-site. First, the installation cost is prohibitively high. Second, these sensors have been engineered without considering the rugged and harsh conditions of a construction site, resulting in error-prone sensing. Third, construction sites are compelled to allocate additional resources in terms of manpower, expenses, and physical spaces to accommodate individual sensors. This research developed an integrated sensing module to measure the environmental information in construction site. The sensing module slashes the installation cost to 3.3%, is robust enough to harsh and outdoor sites, and consolidates multiple sensors into a single unit. The sensing module also supports GPS, LTE, and real-time sensing. The evaluation showed remarkable results including 97.5% accuracy and 99.9% precision in noise measurement, an 89.7% accuracy in dust measurement, and a 93.5% reliability in data transmission. This research empowers the collection of substantial volumes and high-quality environmental data from construction sites, providing invaluable support to decision-making process. These encompass objective regulatory compliance checking, simulations of environmental data dispersion, and the development of environmental mitigation strategies.