• Journal of the Korea Society of Computer and Information
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• v.20 no.1
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• pp.185-195
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• 2015

During hospital peak times of outpatients, an effective mechanism that informs the newly receipted patient information to corresponding medical departments is lacking in current hospital reception systems. Since every department repeatedly searches entire patient reception database in sequential manner to acquire its reception information, this is a significant performance degradation factor in hospital information system. To improve hospital reception system efficiency, we developed two web socket based systems, a primary key transmitting batch system and a reception information transmitting real time system. The former reduced database access time compared to sequential search system as well as kept search time low regardless of receipted patient number. The latter effectively reduced waiting list updating time in request/response patient reception system by eliminating database access.

• Journal of KIISE:Databases
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• v.32 no.5
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• pp.473-486
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• 2005

The Hippocratic database model recently proposed by Agrawal et at. incorporates privacy protection capabilities into relational databases. The authors have subsequenty proposed the Hippocratic XML daかabase model[4], an extension of the Hippocratic database model for XML databases. In this paper, we propose a new concept that we cail the dynamic predicate(DP) for effective access control in the Hippocratic XML database model. A DP is a novel concept that represents a dynamically constructed rendition that tan be adapted for determining the accessibility of elements during query execution. DPs allow us to effectively integrate authorization checking into the query plan so that unauthorized elements are excluded in the process of query execution. Using synthetic and real data, we have performed extensive experiments comparing query processing time with those of existing access control mechanisms. The results show that the proposed access control mechanism improves the wall clock time by up to 219 times over the top-down access control strategy and by up to 499 times over the bottom-up access control strategy. The major contribution of our, paper is enabling effective integration of access control mechanisms with the query plan using the DP under the Hippocratic XML database model.

• Journal of Chest Surgery
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• v.43 no.4
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• pp.356-363
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• 2010

Background: Connexin 43-mediated gap junctional communication plays an important role in atherosclerosis. Numerous studies have demonstrated a correlation between mitral valve annular calcification and atherosclerotic disease. However, the relevance of connexin 43 to mitral valve disease remains unclear. We hypothesized that the mechanism contributing to mitral valve disease is associated with alterations in cell-to-cell communication mediated by changes in Connexin 43 expression. Material and Method: Twenty male New Zealand rabbits were divided into two groups: animals in group 1 (n=10) were fed a normal chow diet, whilst those in group 2 (n=10) received a diet containing 1% cholesterol for 12 weeks. After sacrificing the animals, the mitral valves were excised and analyzed with immunohistochemical staining and Real-time Reverse Transcriptase polymerase chain reaction (real time RT-PCR). Result: Myofibroblasts and macrophages were found concentrated within the endothelial layer on the ventricular side of the leaflet in the cholesterol diet group. Immunohistochemial staining showed elevated expression of connexin43 in the cholesterol diet group. Real-time RT-PCR revealed increased connexin43 mRNA levels in mitral valves from hypercholesterolemic animals. Conclusion: Our finding that connexin43 expression is increased in mitral valves of hypercholesterolemic rabbits suggests that alterations in cell-to-cell communication via connexin43 containing gap junctions play a role in the development of mitral valve disease in hypercholesterolemia.

• Korean Journal of Microbiology
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• v.42 no.4
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• pp.277-285
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• 2006

The most biofilm forming bacteria in catheter, Esctherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were isolated and identified from a patient's catheter occuring catheter-associated urinary tract infection (CA-UTI). We examined mRNA expression and its quantification of AIs synthetic genes encoding signal substance of quorum sensing from each bacterial species in order to elucidated quorum sensing mechanism. Both pure cultures for each bacterial strains and a mixed cultures with three were grown for 24 hr and 30 days. Initial densities to be able to detect mRNA expression oil single strains culture were shown at $2.4{\times}10^5$ CFU/ml, $5.4{\times}10^6$ CFU/ml of E. coli for ygaG and S. aureus for luxS, and at $6.9{\times}10^4$ CFU/ml of P. aeruginosa for rhlI and lasI. Also, in mixed culture of three, initial cell densities of mRNA expression were appear to at $7.3{\times}10^5$ CFU/ml, $1.6{\times}10^7$ CFU/ml of E. coli for ygaG and S. aureus for luxS, and at $2.1{\times}10^5$ CFU/ml of P. aeruginosa for rhlI and lasI. Each AIs synthetic gene was expressed in initial cell density and the mRNA expression of the genes were detected continously during 30 days. And then, the quantification of mRNA expression level of ygaG, rhlI, last, and luxS which were related AIs synthesis was done each time point by real-time RT-PCR. Interestingly, the mRNA levels of ygaG, rhlI, lasI, and luxS from the mixed culture was higher than those from each single strain culture. In the case of E. coli ygaG, the amount of transcript from the mixed culture was at least 30 times for that from single culture. In the case of P. aeruginosa rhlI and lasI, the amount of transcript from the mixed culture was at least 40 times and 250 times for that from single strain culture. In the case of S. aureus luxS, the amount of transcript from the mixed culture was at least 5 times for that from single strain culture. And specially, the mRNA expression of rhlI and lasI of P. aeruginosa showed the highest efficency among four AIs synthetic genes.

• Journal of Gastric Cancer
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• v.20 no.3
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• pp.300-312
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• 2020

Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. Materials and Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_ circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.

• Journal of Digital Convergence
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• v.8 no.3
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• pp.19-37
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• 2010

One of the core issues in the realization of e-government is the effective implementation of a website. The current trend of e-government is developing individual web sites and portals, covering such fields as education, tax, economics, real estate, travel, and health. Evaluating e-government web sites is essential for improving web sites. These principles can be included in usability in a broader concept. Website usability has attracted considerable research on website evaluation and has been regarded as one of the most important criteria for measuring and evaluating websites. Usable e-government web sites make citizens willing to search for helpful information or solve their civil applications. The objective of this paper is to suggest an Analytic Hierarchy Process (AHP) method for evaluating e-government web sites. The proposed AHP method has two special characteristics compared to general AHP approaches. First, all citizens who are e-government users can evaluate both the usability criteria and the web sites, but the weighting mechanism differs between criteria and web sites. Secondly, a pairwise comparison is time-consuming and not cost effective with many criteria. Therefore for evaluating a website with respect to a sub-criterion, we develop checklist items as measure scales with a seven-point rating. This method is efficient as it enable us to avoid the pairwise comparison mechanism between sub-criteria. A new computation procedure for the AHP is developed that combines and synthesizes priorities of portals in the case that two decision maker groups are involved.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.16
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• pp.6947-6951
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• 2015

Background: Oral carcinoma (OC) remains one of the most difficult malignancies to cure. Hesa-A is an Iranian herbal-marine compound that has shown promising anti-tumor properties against various human tumors. However, its mechanism of action remains to be addressed. The present study was conducted to evaluate the effect of two doses of Hesa-A on mRNA expression of erb$\backslash$b2 as a main prognosticator tumor marker for OC in an animal model. Materials and Methods: A total of 60 rats were randomly divided into 5 groups of 12 animals each. Rats in carcinoma groups received 0, 250 and 500mg/kg body weight doses of Hesa-A 3 times a day. The other two groups were considered as treated and untreated control groups. At the end of the experiment, animals were sacrificed and tongue tissues subjected to H and E staining and real time PCR. Results: Our results showed that compared to the control group, erb$\backslash$b2 was over-expressed ~ 30% in the carcinoma group. After treatment with 250mg/kg and 500mg/kg body weight of Hesa-A, erb$\backslash$b2 levels dropped by 24.1% and 3.4 % respectively compared to the control carcinoma group (p<0.01, p<0.0001). Moreover, there was a significant relation between erb$\backslash$b2 mRNA content and observed pathological changes in studied groups (p<0.05). Conclusions: These data provide insight into mechanism(s) by which Hesa-A may improve clinical outcome of oral carcinoma by affecting oncogene erb$\backslash$b2 expression and suggest Hesa-A as an effective chemotherapeutic agent in treatment of HER+tumors.

• International Journal of Aeronautical and Space Sciences
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• v.16 no.2
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• pp.247-253
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• 2015

The components of an aero-engine gas path cannot be monitored in a timely way due to a lack of real-time monitoring technologies. As an attempt to address this problem, we have conducted research on a condition monitoring technology based on the charging characteristics of particles in an aero-engine gas path, and emphatically analyze the formation of particles in an aero-engine gas path, the charging mechanism of carbon particles and the factors that influence the charge quantity and polarity. The verification experiments are performed on the simulated experiment platform and a turbo-shaft engine test bench. The results show the carbon particles' carry charge, and an obvious change in the total electrostatic charge level in the aero-engine gas path due to the increased carbon particles produced by burning or abnormal metal particles; the charge number is related to the size of particles, and the bigger carbon particles carry a negative charge and metal particles carry a positive charge; the change in engine power can lead to an obvious change in the level of electrostatic charge in the gas path, and the change in electrostatic charge results from the extra carbon particles formed in the rich-oil burning process. The research provides a reference for establishing the baseline of electrostatic charge while the engine runs on different power. The study also demonstrates the validity of the electrostatic monitoring technology and establishes a base for developing the application of electrostatic monitoring technology in aero-engines.

• BMB Reports
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• v.38 no.2
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• pp.151-160
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• 2005

By a cDNA array representing 2308 signal transduction related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.9
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• pp.2063-2072
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• 2013

Smart Grid is the next-generation intelligent power grid that maximizes energy efficiency with the convergence of IT technologies and the existing power grid. It enables consumers to check power rates in real time for active power consumption. It also enables suppliers to measure their expected power generation load, which stabilizes the operation of the power system. However, there are high possibility that various kinds of security threats such as data exposure, data theft, and privacy invasion may occur in interactive communication with intelligent devices. Therefore, to establish a secure environment for responding to such security threat with the smart grid, the key management technique, which is the core of the development of a security mechanism, is required. Using a hash chain, this paper suggests a group key management mechanism that is efficiently applicable to the smart grid environment with its hierarchical structure, and analyzes the security and efficiency of the suggested group key management framework.