Journal of Gastric Cancer

  • 제20권3호
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  • Pages.300-312
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  • 2020
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  • 2093-582X(pISSN)
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  • 2093-5641(eISSN)
대한위암학회 (The Korean Gastric Cancer Association)
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Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression

  • Shen, Duo (Medical School, Southeast University) ;
  • Zhao, Hongyu (Department of Clinical Research Center, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine) ;
  • Zeng, Peng (Medical School, Southeast University) ;
  • Song, Jinyun (Department of Clinical Research Center, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine) ;
  • Yang, Yiqiong (Medical School, Southeast University) ;
  • Gu, Xuefeng (Medical School, Southeast University) ;
  • Ji, Qinghua (Medical School, Southeast University) ;
  • Zhao, Wei (Medical School, Southeast University)
  • 투고 : 2020.04.30
  • 심사 : 2020.08.23
  • 발행 : 2020.09.30
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초록

Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. Materials and Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_ circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.

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  2. Roles of ncRNAs as ceRNAs in Gastric Cancer vol.12, pp.7, 2020, https://doi.org/10.3390/genes12071036