• Journal of Veterinary Science
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• 제22권3호
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• pp.40.1-40.12
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• 2021

Background: The microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen. Objectives: The present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees. Methods: A procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration. Results: UR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 10⁴ spores/bee, and the stable detection level was ≥ 2.40 × 10⁵ spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 10⁴ copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min. Conclusions: UR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.

• Smart Structures and Systems
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• 제31권3호
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• pp.275-281
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• 2023

In recent years, technological developments have rapidly increased the number of complex structures and equipment in the industrial. Accordingly, the prognostics and health monitoring (PHM) technology has become significant. The safety assessment of industrial sites requires data obtained by installing a number of sensors in the structure. Therefore, digital twin technology, which forms the core of the Fourth Industrial Revolution, is attracting attention in the safety field. The research on digital twin technology of structures subjected to seismic loads has been conducted recently. Hence, this study proposes a digital twin system that estimates the responses and arbitrary load in real time by utilizing the minimum sensor to a pipe that receives a seismic and arbitrary load. To construct the digital twin system, a finite-element model was created considering the dynamic characteristics of the pipe system, and then updating the finite-element model. In addition, the calculation speed was improved using a finite-element model that applied the reduced-order modeling (ROM) technology to achieve real-time performance. The constructed digital twin system successfully and rapidly estimated the load and the point where the sensor was not attached. The accuracy of the constructed digital twin system was verified by comparing the response of the digital twin model with that derived by using the load estimated from the digital twin model as input in the finite-element model.

• 한국콘텐츠학회논문지
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• 제16권8호
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• pp.671-680
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• 2016

본 연구에서는 통행시간과 점유율의 융합 정보를 이용하는 새로운 실시간 신호제어 알고리즘을 제시하였다. 교통정보시스템의 통행시간 정보를 신호운영에 적용하였으며, 통행시간으로 부터 산정한 포화도를 신호제어에 이용하기 위한 프로세스를 개발하였다. 결정적 지체모형을 이용해 통행시간으로부터 대기행렬 길이를 생성하고, 대기행렬 길이를 다시 포화도로 변환하는 과정이 적용되었다. 또한 통행시간 기반 포화도와 루프검지기 포화도를 융합해 신호시간이 산정되도록 하였다. 신호제어 알고리즘의 효과평가를 위해 미시적 시뮬레이션 분석을 시행하였으며, 과포화 상태에서 기존 루프검지기 기반 실시간 신호제어 대비 최대 27%의 지체 감소 효과를 확인하였다. 또한 과포화 및 검지기 고장상황에 대한 효과적이고, 유용한 대응이 가능함을 확인하였다. 본 연구에서는 교통신호제어시스템과 교통정보시스템의 교통정보 통합이용 방안을 제시하였다는데 의의가 있겠다.

• 대한교통학회지
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• 제19권6호
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• pp.171-182
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• 2001

본 연구는 지점검지체계와 구간검지체계와의 자료합성을 통하여 도심 간선도로 및 지방도로 구간별 효과척도를 산정할 뿐만 아니라 유고검지 및 통행패턴 예측, 네트워크 기종점에 대한 최적/최단 경로를 탐색하는데 기초가 되는 구간 통행시간 추정을 수행한다. 개개 수집원의 자료합성을 위해 퍼지이론과 인공신경 망의 합성모형인 FALEM(Fuzzy Adaptive Learning Estimator for travel time from Multi-information sources)을 개발, 개발된 모형 FALEM에 의해 개개구간의 통행시간을 산출하고 동일시간, 동일구간에서 조사된 실측데이터와의 오차율 비교를 통해 추정된 통행시간을 검증하였다. 테스트 환경은 개발모형에 의해 추정된 구간 통행시간의 적용성을 고려하여 실시간 운영하에서 수행되었다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.668-671
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• 2004

This paper proposes a new method of sensor fusion of an ultrasonic sensor and a vision sensor at the sensor level. In general vision system, the vision system finds edges of objects. And in general ultrasonic system, the ultrasonic system finds absolute distance between robot and object. So, the method integrates data of two different types. The system makes perfect output for robot control in the end. But this paper does not propose only integrating a different kind of data but also fusion information which receives from different kind of sensors. This method has advantages which can simply embody algorithm and can control robot on real time.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.798-802
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• 2003

The robustness and reliability of vision algorithms is the key issue in robotic research and industrial applications. In this paper, the robust real time visual tracking in complex scene is considered. A common approach to increase robustness of a tracking system is to use different models (CAD model etc.) known a priori. Also fusion of multiple features facilitates robust detection and tracking of objects in scenes of realistic complexity. Because voting is a very simple or no model is needed for fusion, voting-based fusion of cues is applied. The approach for this algorithm is tested in a 3D Cartesian robot which tracks a toy vehicle moving along 3D rail, and the Kalman filter is used to estimate the motion parameters, namely the system state vector of moving object with unknown dynamics. Experimental results show that fusion of cues and motion estimation in a tracking system has a robust performance.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 춘계학술대회 논문집
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• pp.1055-1058
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• 2003

The robustness and reliability of vision algorithms is the key issue in robotic research and industrial applications. In this paper robust real time visual tracking in complex scene is considered. A common approach to increase robustness of a tracking system is the use of different model (CAD model etc.) known a priori. Also fusion or multiple features facilitates robust detection and tracking of objects in scenes of realistic complexity. Voting-based fusion of cues is adapted. In voting. a very simple or no model is used for fusion. The approach for this algorithm is tested in a 3D Cartesian robot which tracks a toy vehicle moving along 3D rail, and the Kalman filter is used to estimate the motion parameters. namely the system state vector of moving object with unknown dynamics. Experimental results show that fusion of cues and motion estimation in a tracking system has a robust performance.

• 한국소음진동공학회논문집
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• 제14권11호
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• pp.1091-1100
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• 2004

This paper presents an approach for the monitoring and detection of faults in induction machine by using data fusion technique and Dempster-Shafer theory Features are extracted from motor stator current and vibration signals. Neural network is trained and Hosted by the selected features of the measured data. The fusion of classification results from vibration and current classifiers increases the diagnostic accuracy. The efficiency of the proposed system is demonstrated by detecting motor electric and mechanical faults originated from the induction motors. The results of the test confirm that the proposed system has potential for real time application.

• International Journal of Control, Automation, and Systems
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• 제1권4호
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• pp.444-452
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• 2003

An integration system with multi-measurement sets can be realized via combined application of a centralized and federated Kalman filter. It is difficult for the centralized Kalman filter to remove a failed sensor in comparison with the federated Kalman filter. All varieties of Kalman filters monitor innovation sequence (residual) for detection and isolation of a failed sensor. The innovation sequence, which is selected as an indicator of real time estimation error plays an important role in adaptive mechanism design. In this study, the centralized Kalman filter with adaptive measurement fusion is introduced by means of innovation sequence. The objectives of adaptive measurement fusion are automatic isolation and recovery of some sensor failures as well as inherent monitoring capability. The proposed adaptive filter is applied to the GPS/SDINS integration system with an additional sensor. Simulation studies attest that the proposed adaptive scheme is effective for isolation and recovery of immediate sensor failures.

• International Journal of Aeronautical and Space Sciences
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• 제11권1호
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• pp.31-40
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• 2010

For weapon cueing and Head-Mounted Display (HMD), it is essential to continuously estimate the motion of the helmet. The problem of estimating and predicting the position and orientation of the helmet is approached by fusing measurements from inertial sensors and stereo vision system. The sensor fusion approach in this paper is based on nonlinear filtering, especially expended Kalman filter(EKF). To reduce the computation time and improve the performance in vision processing, we separate the structure estimation and motion estimation. The structure estimation tracks the features which are the part of helmet model structure in the scene and the motion estimation filter estimates the position and orientation of the helmet. This algorithm is tested with using synthetic and real data. And the results show that the result of sensor fusion is successful.