• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.236-244
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• 2014

The gene encoding ${\alpha}-\small{L}$-arabinofuranosidase (AFase) from Paenibacillus sp. DG-22 was cloned, sequenced, and expressed in Escherichia coli. The AFase gene (abfA) comprises a 1,509 bp open reading frame encoding 502 amino acids with a molecular mass of 56,520 daltons. The deduced amino acid sequence of the gene shows that AbfA is an enzyme consisting of only a catalytic domain, and that the enzyme has significant similarity to AFases classified into the family 51 of the glycosyl hydrolases. abfA was subcloned into the pQE60 expression vector to fuse it with a six-histidine tag and the recombinant AFase (rAbfA) was purified to homogeneity. The specific activity of the recombinant enzyme was 96.7 U/mg protein. Determination of the apparent molecular mass by gel-filtration chromatography indicated that AbfA has a tetrameric structure. The optimal pH and temperature of the enzyme were 6.0 and $60^{\circ}C$, respectively. The enzyme activity was completely inhibited by 1 mM $HgCl_2$. rAbfA was active only towards p-nitrophephenyl ${\alpha}-\small{L}$-arabinofuranoside and exhibited $K_m$ and $V_{max}$ values of 3.5 mM and 306.1 U/mg, respectively. rAbfA showed a synergistic effect in combination with endoxylanase on the degradation of oat spelt xylan and wheat arabinoxylan.

• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.21-27
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• 1998

The xylA gene of Bacillus stearothermophilus encoding the major ${\beta}$-xylosidase was previously cloned and sequenced. In the present study we examined the regulation of the cloned xylA gene expression in Bauillus subtilis MW15 carrying the xylA::aprA fusion plasmids. The induction of the fused xylA gene expression remained uninfluenced by any of the carbon sources tested but the gene expression was repressed about 2-3 fold in the presence of glucose. Two CRE-like sequences (CRE-1: nucleotides ＋ 124 to ＋136 and CRE-2: ＋247 to ＋259) were recognized within the reading frame region of the xylA gene. The deletion experiments showed that the CRE-2 sequence had a role in catabolite repression (CR) as a true CRE of the xylA gene, but the CRE-1 had no effect on CR of the xylA gene expression. Surprisingly, the deletion of the CRE- 1 sequence reduced about 2~3 fold of the expression of the xylA fused gene. The repression ratios of the xylA gene expression were estimated to be about 0.4 from the assay of subtilisin activity, and about 0.3 at the level of transcription by determining the amounts of xylA transcripts in B. subtilis. While, the level of CR of the xylA gene was assessed to be about l0-fold in previous work when the relative amounts of the xylA transcripts were measured in B. stearothermophilus.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1387-1390
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• 2005

Recently, foreign utilities emphasize the importance of the value-added services based on Information Technology(IT) as one of the strategic technologies for establishing a new power system in the future digital society. They develop many different types of the value-added services and apply the systems for customer. In domestic case, the data from the Automatic Meter Reading (AMR) System is used only for calculating the tariffs. Data from the AMR system can be strategic assets for utilities to provide the value-added services for customer. Development of the value-added services for utilities and customer needs processing and managing the AMR data. In this paper, the energy consulting service, which is adequate to new power system environment, is suggested for development of the value-added services. The application of the suggested service will bring the effect of reducing the monthly bill for customer. Also the service will give not only the effective demand side management(DSM) and load control, but also reduction of the investment for utilities.

• Speech Sciences
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• v.10 no.2
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• pp.191-203
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• 2003

This study examined the effect of vocal loudness on nasalance measures, under the conditions of three sentence patterns (i.e., Oral sentences, Mixed sentences, Nasal sentences). The vocal loudness level was classified into soft voice (55 dB), medium voice (65 dB) and loud voice (75 dB). The participants in the present study were 30 normal adults (male: female =1:1). Kay's Nasometer 6200 was used to measure nasalance and Sound level meter was used to adjust the loudness level. The results of the present study are as follows. Firstly, the change in vocal loudness is in the following. In the Oral sentence stimuli, the loud voice for both male and female showed the highest nasalance degree, and the medium voice the lowest level. In the Mixed and Nasal sentence stimuli, however, male participants showed the highest degree of nasalance in the soft voice, and the lowest degree in the loud voice, and female showed the highest degree of nasalance in the soft voice and the lowest in the medium voice. Secondly, when each subject's nasalance scores were ranked in a ordered manner, noticeable tendency. Lowest nasalance score occurred in the loud voice and the highest nasalance score was recorded in the soft voice during participants' reading of the Nasal sentences. However, it was hard to find such pattern in the Oral sentences. It is assumed that velopharyngeal function could be related to these findings. Furthermore, the findings associated with vocal loudness may have diagnostic as well as clinical implications.

• Journal of Korean Academy of Nursing
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• v.2 no.1
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• pp.175-188
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• 1971

More and Tore emphasis is put on moral treatment in the care of psychiatric patients. Methods employed for moral treatment rake use of therapeutic activity for the mentally ill patient. In some psychiatric hospitals all of the therapeutic activities are categorized as occupational therapy, while in others these activities are departmentally categorized -recreational, musical, occupational and bibliotherapy. All of these constitute separate departmental therapies. By research, it is known at this time, that the above activities have been of great help in personality integration, reeducation and early recovery of patients, as well as in their social adjustment after discharge. The goal of these therapeutic activities incorporate the goals set by healthier interpersonal relationship and psychotherapy. Put the therapeutic activities require a prolonged time of therapy for effectiveness, economic support, special trained personnel, careful planning, guidance and proper evaluation. Therefore this treatment is a very complicated one. But it may be the most profitable therapy for certain patients. One true fact about this treatment is, that simple recreation, occupation, music, reading, etc., make the patients life in the ward more interesting and have a good effect on the interpersonal relationship between patients. Thus it has been proved that this kind of treatment demands the active and full participation of the personnel and the full support of the hospital administration.

• Korean Journal of Child Studies
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• v.33 no.6
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• pp.51-69
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• 2012

The purpose of this study was to investigate the differences in children's daily experience and school adjustment of elementary school according to children's gender and self-care and to explore the relationship between children's daily experience and school adjustment. Data were collected from the NYPI panel survey of 2010. The data were analyzed by means of Cronbach's ${\alpha}$ tests, means, standard deviations, Two-way ANOVAs, and Pearson's Correlation. The results of this study were as follows : 1. There were some significant differences by gender and self-care in daily experience. Girls spent more time in watching TV but less time in games than boys. And self-care children spent less time in learning and reading, but more time in game and TV watching. Also there was a interaction effect of learning experience by gender and self-care. 2. There were some significant differences by gender and self-care in school adjustment. Girls showed more positive school adjustment than boys. And children who are cared for by their parents showed more positive learning and school rule adjustment. 3. There were some significant relationship between children's daily experience and school adjustment.

• The Mathematical Education
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• v.55 no.4
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• pp.503-522
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• 2016

The purpose of this study is to investigate the effects of peer-to-peer relay feedback strategy on achievement in mathematical problem solving and mathematical communication ability. The experimental instruction was given to 99 students. They were divided into two groups. In this research a strategy of peer-to-peer relay feedback is developed. To enhance lateral communication, students were instructed according to the strategy to read open-type problems, to write mathematical ideas individually and to share their ideas and get feedback in relay method with peers within a small subgroup. The results are as follows. 1) Mathematics achievement assessments were carried six times in the cleanup phases of the class. There was no difference at the 1st test. 2) The second t-test of mathematical communication abilities showed that there was meaningful difference and that peer-to-peer relay feedback was effective. But there was no difference on reading. 3) Peer-to-peer relay feedback strategy has a positive impact.

• English Language & Literature Teaching
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• no.3
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• pp.13-42
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• 1997

The purpose that the curriculum appropriate for the student's level pursues is to offer to a student the curriculum that can satisfy the student's interest, aptitude, and requirements. This curriculum has recently enforced all over the country, but the careful research and the plan for verification on its effect are required so that it may accomplish its purpose. This study, centered on the reading comprehension ability of Freshman English of the High School, aims to suggest a more desirable procedure for organizing and enforcing the curriculum appropriate for the students' level. In order to provide the best curriculum appropriate for the student's level. (1) the diagnosis of the individual student's level should be made first ; (2) the syllabus focused both on the grammar and on the communicative functions should be prepared ; (3) the teaching material on the basis of the syllabus should be selected considering the student's interest. and requirements. The new method of teaching-learning English should also be developed with the enforcement of the new curriculum discussed here. The curriculum appropriate for the student's level has many kinds of problems but these disadvantages should be overcome for providing the better educational system for the individual student.

• Annual Conference on Human and Language Technology
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• 2001.10d
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• pp.226-232
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• 2001

본 연구는 결속장치(cohesive devices)가 과학텍스트의 읽기 속도와 내용 이해에 끼치는 영향에 대해 연구하였다. 연구의 목적을 위한 실험을 통해서 먼저, 텍스트의 문단별 읽기 시간을 측정하여 온라인 처리 과정을 검토하였고, 둘째, 회상과 재인 검사를 실시하여 오프라인 상태에서의 이해도를 조사하였다. 이 연구의 재료로 사용된 텍스트는 번개 생성과정에 대한 과학텍스트로서, 반복, 지시사, 정박(anchoring), 인과적 접속사 등의 결속장치를 이용하여 응집성(coherence)의 강도를 높고, 낮게 조작하였다. 실험 결과, 결속장치가 길속장치의 종류와 지엽적 응집성의 강도에 따라 과학텍스트 읽기와 이해에 선택적으로 영향을 끼친다는 것을 발견하였다. 첫째, 인과적 접속사는 읽기 시간에는 영향을 주지 않는 반면, 이해를 촉진했는데, 이 긍정적 효과는 과제의 종류에 따라 다르게 나타났다. 즉, 회상 검사 결과에서는 인과적 접속사가 쓰인 모든 문단에서 유의한 차이가 나타났으나, 재인 검사에서는 유의한 차이가 부분적으로만 나타났다. 둘째, 반복 결속장치는 다른 결속장치와 같이 발생할 경우에만 읽기 시간과 이해를 부분적으로 촉진하는 것으로 나타났다. 셋째. 정박 결속장치의 영향은 읽기와 이해 두 처리 과정에 모두 선택적으로 영향을 준 것으로 나타났다. 인과적 접속사와 함께 쓰인 문단의 경우에는 회상 검사에서만, 반복 결속장치가 함께 쓰인 문단에서는 회상, 재인 검사에서 모두 긍정적 영향을 준 것으로 관찰되었다.

• Proceedings of the Optical Society of Korea Conference
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• 2007.07a
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• pp.275-276
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• 2007

A multiplex recording and reading technique in photopolymer are presented In order to record the polarization gratings, polarization-sensitive materials, in which linear birefringence is induced by irradiating the polarized light, are necessary. We checked orthogonal and independence of laser. The value of the photoinduced linear polarization had effect on diffraction properties in the holographic gratings. The grating strengths of two polarization are investigated and the relevant parameters for equal diffraction intensity readout are optimized.