• 한국수산과학회지
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• 제46권5호
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• pp.534-539
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• 2013

To secure the biological safety of live fish and shellfish for raw consumption, the germicidal effects of electrolyzed seawater were evaluated. Upon direct exposure to electrolyzed seawater, coliform group bacteria were killed and decreased to undetectable levels after 1 day. The physicochemical characteristics of the seawater were stable during the test period. A byproduct of chlorine disinfection, trihalomethane, was not generated by the electrolysis of seawater. Vibrio parahaemolyticus infection in a live fish was effectively resolved by electrolyzed seawater and became undetectable after 12-36 h of treatment. Bioaccumulation of coliform group and fecal coliform bacteria in live oysters Crassostrea gigas was removed within 18 h of treatment. This study demonstrated that electrolyzed seawater is an effective and safe germicidal agent for the traditional retail market and can help to prevent outbreaks of foodborne disease associated with the consumption of raw fish and shellfish.

• 한국양식학회지
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• 제19권2호
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• pp.84-89
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• 2006

사료내 다양한 녹차 원료 첨가에 따른 넙치 유어기의 성장, 면역성 및 세균 공격성에 미치는 영향을 조사하였다. 녹차엑기스를 첨가한 사료를 공급한 실험구에서 어체중증가는 대조구와 유의적인 차이는 없었지만, 녹차 생잎, 건조잎 또는 부산물을 첨가한 실험구에 비하여 유의적으로 우수한 것으로 나타났다. 그리고 녹차엑기스를 첨가한 실험구에서의 사료전환효율은 대조구와 유의적인 차이는 없었지만, 녹차 생잎, 건조잎 또는 부산물을 첨가한 실험구에 비하여 유의적으로 우수한 것으로 나타났다. 녹차 건조잎과 엑기스를 첨가한 실험구에서 Iysozyme 활성은 유의적인 차이는 없었지만, 녹차 생잎과 부산물을 첨가한 실험구에 비해서는 유의적으로 높은 Iysozyme 활성이 나타났다. 3시간에서의 세균 살해능은 녹차 건조잎과 엑기스를 첨가한 실험구에서 살해능이 녹차 생잎과 부산물 또는 대조구에 비하여 유의적으로 낮게 나타났다. 또한 6시간에서의 세균 살해능은 녹차 엑기스, 건조일, 생잎, 부산물 및 대조구의 순으로 나타났으며 , 대조구에 비하여 엑기스, 건조잎 및 생일은 유의적으로 낮게 나타났다. 세균 공격실험 결과, 복강 주사 2일째부터 넙치의 폐사가 나타나기 시작하여 4일 동안 폐사는 계속되었으며 녹차 생잎과 부산물을 첨가한 실험구에서의 누적폐사율은 대조구에 비하여 높게 나타났으며 녹차 건조잎과 엑기스를 첨가한 실험구에서는 대조구에 비하여 낮은 누적폐사율을 보였으나 실험구간에 유의적인 차이는 없었다. 따라서 녹차엑기스와 건조잎의 사료내 첨가는 넙치의 면역성 향상과 세균공격성에 내성 향상 면에 있어서 아주 효과적인 것으로 판단된다.타내었고 $25^{\circ}C$ 실험구의 경우 대조군과 비교하여 감소하는 경향을 나타내었다. CAT에서는 수온 스트레스 직후 10 및 $25^{\circ}C$ 실험구에서는 대조군과 비교하여 낮은 활성을 나타내었으며, 시간의 지남에 따라 회복하는 경향을 나타내었다. 또한, $15^{\circ}C$ 실험구의 경우에는 6 h째에 가장 높은 CAT 활성을 나타내었으며, 이 후 차츰 회복하는 경향을 나타내었고, $30^{\circ}C$ 실험구의 경우에는 시간의 지남에 따라 유의적으로 활성이 낮아지는 경향을 나타내었다. HSP70 mRNA의 발현은 대조군($20^{\circ}C$)과 비교하여 $25^{\circ}C$ 48 h째 실험구를 제외한 모든 실험구에서 유의적으로 높게 발현되었다. 이상의 결과로, $20^{\circ}C$에서 순치된 시볼트전복은 급격한 수온 스트레스에 대해 많은 스트레스 요인으로 작용하는 것으로 나타났으며, 수온 스트레스에 대한 생리학적 방어 기작이 분자 레벨인 HSP70 mRNA에서는 신속히 발현되어 스트레스에 대처하지만, SOD나 CAT와 같은 항산화 효소의 발현은 다소 늦게 작용하는 것으로 나타났다. 그러나, 시간의 지남에 따라 $5^{\circ}C$ 내외의 스트레스와 저수온 스트레스의 경우에는 비교적 안정화되는 것으로 보여지며, $10^{\circ}C$ 이상의 고수온에 노출되었을 경우에는 생리학적 방어기작이 한계점에 이르러 더

• 농촌의학ㆍ지역보건
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• 제8권1호
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• pp.44-65
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• 1983

As a part of the epidemiological studies of clonorchiasis, this study was conducted to evaluate the current endemicity and the natural transition of the Clonorchis infection in Gimhae Gun and delta area a high endemic area in Korea in recent years, prior to the introduction of praziquantel which will eventually influence the status of the prevalence. The data obtained in this study in 1983 were evaluated for natural transition of the infection in comparison with those obtained 16 years ago in 1967 by the author(Kim, 1974). The areas of investigation, villages and schools surveyed, methods and techniques used in this study were the same as in 1967, except for the contents of the questionnaire for raw freshwater fish consumption by the local inhabitants. 1) The prevalence rate of clonorchiasis in the general population of the villages was 48.1% on the average out of a total of 484 persons examined. The average of those of the riverside-delta area was 65.2% and 43.0% in the inland area. Among the schoolchildren, the prevalence rate was 8.2% on the average out of a total of 1,423 examined. By area, the prevalence rate was 10.8% in the riverside delta area and 2.8% in the inland area. By sex, difference in the prevalence was seen only in the inhabitants of the inland area showing 52.4% in the male and 33.5% in the female. 2) In the natural transition of the infection, the prevalence rate among the inhabitants has decreased from 68.8% in 1967 to 48.1% in 1983, and in the schoolchildren from 56.4% in 1967 to 8.2% in 1983. The reduction rate was higher in the riverside-delta area than in the inland area. 3) In the prevalence rate by age, 11.9% was first seen in the 5-9 age group and the rate gradually increased up to 75.0% in the 50-59 age group. By sex, the rate was higher in the male than in the female in the 20-29 age group and over. 4) In the natural transition of the prevalence rate by age, the reduction rate of the infection during the past 16 years was greater in the younger age groups up to the 40-49 age group and reached the same level in the age group 50-59. Reduction was seen again in the age group over 60s. By sex, the reduction rate was greater in the female than in the male in the 20-29 age group and over. By area, the reduction rate was greater in the riverside delta area than in the inland area, particularly in the young age groups. 5) In the intensity of the infection among the cases, the mean egg out-put per mg feces per infected cases(EPmg) in the inhabitants was 6.3. EPmg of those of the river-side-delta area was 15.4 and that of the in-land was 2.8. On the other hand, in the schoolchildren, EPmg was 3.2, and no difference was seen between the two areas, the river-side-delta area and the inland area. 6) In the transition of the intensity of the infection by area, EPmg among the inhabitants inexplically increased from 7.8 in 1967 to 15.4 in 1983. This was probably caused by uneven specimen collection in the process of sampling the population. EPmg of the inhabitants in the inland area and those of the schoolchildren of both riverside delta and inland areas showed a similar decrease in the past 16 years. 7) The intensity of the infection by age showed a relatively low level in the 20-29 age group and below, and EPmg 5.1-9.5 was seen in the 30-39 age group and over. Sex, Epmg was 5.8 in the male and 4.7 the female. By in 8) In the transition of the intensity of the infection, EPmg decreased from 6.2 in 1967 to 5.4 in 1983. By age, in contrast to the figures of 1967 in which EPmg gradually increased with some fluctuation from 1.1 in the 0-4 age group to peak 10.5 in the 50-59 age group, in 1983 lower intensity of the infection was seen in the age group from 10-14 to 20-29 with the EPmg range of 0.6-2.7. 9) In the distribution of the clonorchiasis cases by the range of EPmg value, 43.2% of the cases were in 0.1 0.9 and 34.6% in 1.0-4.9. As a whole by cumulative percent, 44.6% of them were under 0.9 as light infection and 86.1% of them under 9.9 up to moderate infection. By sex, no difference was seen in Epmg. 10) In the transition of the distribution by the range of Epmg, the cases were distributed up to the range 80.0-99.9 in 1967 and to 60.0-79.9 in 1983. By cumulative percent, in the range of 0.1-0.9 and less, light infection, 34.3% of them were distributed in 1967 and 44.6% in 1983 with about 10% increase. In the range of 5.0-9.9 and less, up to moderate infection, 83.2% in 1967 and 86.1% in 1983 of the cases were seen, respectively. 11) The practice of raw freshwater fish consumption among the inhabitants seems to have decreased in recent years. Those who admitted to raw freshwater fish consumption in the last two years among the infected inhabitants were 59.3%, although 86.8% of them professed to have experience with raw freshwater fish consumption. 31.7% of those who have had experience of the raw freshwater fish consumption denied any further consumption in recent years. From an interview of 543 school-children, 24.1% of them admitted to an experience of raw freshwater fish consumption. However, those who have practised in the past two years comprized 17.9%. Those who denied raw freshwater fish consumption in recent years among those who had such experience were 26.0% out of 131 interviewed. The rate of raw freshwater fish consumption in both inhabitants and schoolchildren were higher in the male than in the female. On the contrary, the rate of those who did not practise in recent years among those who had experience of raw freshwater fish consumption was higher in the female than in the male. 12) The major reason for the reduction of raw freshwater fish consumption among the local inhabitants was the risk of the fluke infection. However, it has become apparent that such change of taste has resulted from water pollution impact which has affected throughout the areas of the freshwater systems in this locality since last several years. 13) In animal survey, Clonorchis infection was seen in 14.8% of 88 dogs examined and 3.7% of 27 house rats examined. It was noted that populations of dogs and cats have increased in the villages surveyed. Although the prevalence rate was lower in the present survey than those of 1967, the significance of the animals as the reservoir host has not changed. 14) Prevalence rate of Clonorchis infection by cercariae in the first intermediate host, Parafossarulus manchouricus, was 0.6% out of 517 snails examined. The infection rate was lower in comparison with 2.3% out of 2,124 examined in 1967. Moreover, sharp decreases in number and distribution of the intermediate host snails in many watershed areas of the huge freshwater systems in this locality seemed to reduce transmission of Clonorchis in connection with the intermediate host stage of its life cycle. 15) Clonorchis infection in the second intermediate fish hosts was relatively low. The mean number of Clonorchis metacercaria per fish in Pseudorasbora parva was 517 in 1983, whereas it was 1943 in 1968 through 1969. Environmental water pollution has also caused the decreased fish population density in these areas, and this has also apparently affected to the practice of raw freshwater fish consumption among the local inhabitants. 16) In conclusion, endemicity of Clonorchis infection in Gimhae Gum and delta area of the Nagdong River has sharply decreased during the past 16 years. The major cause of the regressive transition of the infection was the water pollution of the land water systems of this locality. The pollution has upset the ecosystems comprizing of the intermediate hosts of Clonorchis in many areas, and also affected to a significant extent to the discontinuance of the local inhabitants for raw freshwater fish consumption.

• 한국정보통신학회논문지
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• 제23권1호
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• pp.39-46
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• 2019

본 연구에서 외래어종 퇴치를 위한 시스템 개발에 앞서 물 안의 어류 이미지를 CNN으로 학습하여 어종을 분류하는 알고리즘을 제안하고자 한다. CNN 학습을 위한 원데이터(raw data)는 각 어종에 대해 직접 촬영한 영상을 사용하였으며, 어종 분류성능을 높이기 위해 영상 이미지의 개수를 늘린 데이터세트 1과 최대한 자연환경과 가까운 영상 이미지를 구현한 데이터세트 2를 구성하여 학습 및 테스트 데이터로 사용하였다. 4가지 CNN의 분류성능은 데이터세트 1에 대해 99.97%, 데이터세트 2에 대해 99.5% 이상을 나타내었으며, 특히 데이터세트 2를 사용하여 학습한 CNNs이 자연환경과 유사한 어류 이미지에 대해서도 만족할 만한 성능을 가짐을 확인하였다. 그리고 4가지 CNN 중 AlexNet이 성능에서도 만족스러운 결과를 도출하였으며, 수행시간과 학습시간 역시 가장 짧아 외래어종 퇴치를 위한 시스템 개발에 가장 적합한 구조임을 확인하였다.

• 수산해양교육연구
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• 제24권5호
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• pp.609-615
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• 2012

A histo-pathogical examination was carried out to evaluate the effect of commercial extruded pellet (EP) and a raw fish moist pellet (MP) diet on the health of juvenile rockfish cultured in marine net-cage for 7 months. Fish were distributed randomly to each net cage as a group of 76,000 fish (initial mean body weight 5.9 g). After 2 months, the hypertrophy or swelling of liver parenchymal cells was identified in most individuals and lasted until 7 months. Livers in EP fed group frequently showed hypertrophic parenchyma and fatty change with occasional atrophic cells. However, after 4 months, lymphocytic infiltration in splenic parenchyma was seen in a number of individuals. In addition, the gastric glandular epithelium was atrophied and in the lumen of renal tubules protozoan parasites were frequently identified but there was no correlation with the type of feed. Moreover, juvenile rockfish on EP diet showed gross and microscopic hypertrophy of the liver which would be due to oversupply of feed. Severe hepatic cellular hypertrophy or swelling could lead to the damage of microcirculation. Especially fatty change and atrophic change of liver could be the result from the damage, which could be responsible for immunological problem. Lymphocytic infiltration of spleen on the MP diet suggests that juvenile rockfish could be frequently exposed to infectious antigens.

• 한국식품조리과학회지
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• 제29권5호
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• pp.533-541
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• 2013

The purpose of this article was to study the influence of tangible clues of raw-fish restaurants on consumers' emotional responses and repurchasing behavior in eastern area of Kangwon-province. In this study frequency analysis, reliability analysis, factor analysis and path analysis(SEM) were applied to analyze the data. Through path analysis tangible clues' performances had generally significant influences upon consumers' emotional responses to buy products of raw-fish restaurants. According to the results, exterior facility had positive influence on pleasure(p<0.001) and arousal(p<0.01). Interior facility had positive influence on pleasure(p<0.001) and arousal(p<0.001), but negative on dominance(p<0.01). And the others had positive influence on pleasure(p<0.05). Press copy had positive influence on pleasure(p<0.001) and arousal(p<0.001). And Pleasure and arousal had positive influence on repurchasing behavior(p<0.001). But Dominance didn't have significant influence on repurchasing behavior. As a result, we studied that the tangible clues was an effective marketing tactic to maintain and increase consumers' positive emotional reponses to repurchase at restaurants. Therefore it is finally needed that dining-out corporations manage tangible clues in a customer-maintaining without additional costs, and especially to use mixing tangible clues each other in some suitable condition.

• 한국식생활문화학회지
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• 제21권3호
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• pp.303-310
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• 2006

This study was performed by questionnaire to investigate the satisfaction and preference on the menu of Japanese restaurant customers in Seoul. The subjects were consisted of 386 participants in Seoul. The results are as follows: Respondents consider that the preferred menu of Japanese restaurants' foods is menus such as 'assorted raw fish', 'sushi', and 'dessert' rather than menus such as 'jin mi', 'seasonal appetizer', 'raw fish with vinegar' and 'clean soup'. The higher intake Japanese cuisine menus was 'dessert' (3.71 point), 'noodle and soup' 'sushi' 'fried dishes', and but 'jin mi' (1.91 point), 'seasonal appetizer' scored the lowest. In result of analyzing difference between importance and satisfaction of the menu, importance is much higher than satisfaction. In terms of IPA analysis on Japanese cuisine menu, it was noteworthy that the 2nd quadrant with high fulfillment but low importance for customers included 'noodle and soup'. The menus that need continuous keeping management with high importance and high fulfillment included 'assorted raw fish', 'roasted dishes', 'fried dishes', 'beef and seafood casserole', 'sushi', 'deopbap', 'dessert'.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2002년도 추계 수산관련학회 공동학술대회발표요지집
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• pp.116-117
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• 2002

Olive flounder and Black rockfish comprises around 90％ of the total fish culture in Korea. Most of the olive flounder is consumed as raw fish meat “saengseonhoe”, as like a sashimi in Japan. Also, texture is particularly a important factor which determine the quality of raw fish meat in Korea. Many works have attempted to improve of texture such as arai, electrical stimulation and compulsory exercise of fishes. (omitted)

• 수산경영론집
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• 제34권2호
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• pp.91-107
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• 2003

Distribution of fish products from Tongyoung Marine ranching can be classified by three routes such as street-stall, live fish transportation vehicles, and wholesale markets neighboring unloading ports. These methods of distribution, however, have been restricted by limited distribution right, difficulties to differentiate fish prices from other surfaces, simple marketing channels. The ratio of cultured live fish circulated in market is increasing while naturally caught live fish is decreasing and the fresh fish shows a little of increasing rate. Consumers purchasing routes mainly depend on the live fish transportation merchants. For fresh fish traditional market plays an important role in trade. Convenience for consumers and quality of products are main factors in making decision of purchases. Bargaining power, however, belongs to the live fish transportation merchants. The demand of special markets for live fish was very strong, and the convenience and quality are relatively important required factors. Catch from Tongyoung Marine ranching has very good reputation as the possibility of being a good brand. Expecting possibility of quality differentiation was higher than price differentiation specially. The possible conclusion of a contract of a supply was suspicious however. Preliminary quality evaluation revealed that the catch is better than the cultured but worse than naturally grown fish. A merchandising is to be in a better position in the formation of prices by giving $\ulcorner$brand image$\lrcorner$ to potential consumers. The target markets are retail stores such as restaurants for raw fish and final consumers. The staple markets are retail stores. Possible items of products are live fish, fresh fish for cook, and fresh fish for raw fish. It is necessary for the catch to be informed as new functional products that have been improved in safety and quality, since the product positioning is similar but not well known to consumers. To secure a brand it is required to register a trademark, eco-label product design or packing, use real name in tranction, introduce recall system, and put label. Price higher than naturally grown live fish should be targeted. Establishing broad distribution channel, wholesale market, franchise are required. To secure enough catch and control shipment of products facilities of containing live fish are necessary. Instead of dealing with live fish only, it would be better to. sell fresh fish and live fish simultaneous. Strategically promotion focuses on advertisement of Marin ranching at first and then focuses on the catch from the marine ranching.

• 한국식품위생안전성학회지
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• 제19권2호
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• pp.84-96
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• 2004

본 연구는 우리나라에서는 새로운 형태의 생선회인 싱싱회 생산업체에 HACCP시스템이 정착되는 것을 지원하기 위하여 작업자가 쉽게 활용할 수 있는 SSOP(Sanitation Standard Operating Procedure:)프로그램을 개발하여 이를 활용하여 SSOP프로그램이 없이 일반적인 방법으로 식품을 위생적으로 생산하려고 하는 것과 SSOP프로그램을 제정하여 이를 활용하여 작업할 때의 위생 이행 효과를 식품접촉표면 시료와 작업자 개인위생 시료를 세균 검사하고, 위생 점검표로 위생 감사하여 그 결과를 합산하고 통계 분석하여 SSOP프로그램의 활용 성과를 확인한 것이다. 현재 정부에서 사용하는 5때 항목의 적합/부적합 여부로 부적합이 6개 이상이면 위생불량으로 판정하는 위생감사와 달리 위생감사 내용을 점수로 계량화하여 70％로 환산하고 샘플링의 세균 검사 결과도 계량화하여 이를 30％로 환산하여 두 결과를 합산하여 100점으로 하였다 그 결과 60점 이하이면 위생 불량으로 행정제재, 61-75점이면 보통, 76-85점이면 양호, 86점 이상이면 우수로 판정하고 SSOP프로그램을 활용한 위생 실태를 통계적으로 분석하였다. 새로 개량한 위생 점검표에 의한 위생 이행과 위생 시설 구비 여부를 점검한 환산 점수는 활용 전 11.8％에서 활용 후 88.6％로 위생 등급이 두 등급 향상되었다 세균 검사 결과를 점수로 환산한 결과도 57.5％에서 98.2％로 개선되었으며 유의한 차이가 있었다. 위 두 부분(위생감사와 세균 검사 결과)의 환산 점수를 각각 70％와 30％로 환산하여 총합 점수로 분석한 SSOP 활용 전 후의 성과도 66.4점에서 93.4점으로 향상되었다 상기 결과로 당 연구 대상 업체는 HACCP시스템의 올바른 정착에 필요한 위생의 8가지 핵심 내용이 관리 하에 있음이 입증되었다.