• Title/Summary/Keyword: raw egg

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Effect of Dietary Inclusion of Medicinal Herb Extract Mix in a Poultry Ration on the Physico-chemical Quality and Oxidative Stability of Eggs

  • Liu, X.D.;Jang, A.;Lee, B.D.;Lee, S.K.;Lee, M.;Jo, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.3
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    • pp.421-427
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    • 2009
  • A mixture of three dietary medicinal herb extracts (MHE, mulberry leaf:Japanese honeysuckle:goldthread = 48.5: 48.5:3.0) was prepared as an additive of hen's feed. One hundred-eight, 28-wk-old Lohmann Brown hens were assigned randomly with three levels of MHE in the diet (0, 0.3, and 1%). Hens were fed for 6 wks and eggs were collected in the 6th week, and stored at $4^{\circ}C$ for 14 days to investigate the effect of MHE on the quality and oxidative stability of eggs. Internal quality of the egg including weight, shell color, albumen height, yolk color, shell weight, shell thickness, and Haugh units was not different among the dietary treatments. The oxidation stability of raw and cooked egg was determined by 2-thiobarbituric acid reactive substances (TBARS) value, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and 2,2'-azinobis(3-ethylbenzonthianoline-6-sulfonic acid) ($ABTS^{+}$) radical reducing ability. Results indicated that TBARS value at day 0 and $ABTS^{+}$ radical reducing ability of eggs from hens fed MHE were higher than from the control group. However, DPPH radical scavenging activity showed no difference in both raw and cooked samples. Results of the present study indicate that dietary MHE may slightly enhance the oxidative stability of eggs.

Effects of Liquid Rooster Sperm on Reproductive Ability in Chicken (정액의 액상보존이 닭의 정액성상 및 수정율에 미치는 영향)

  • 김학규;나재천;최철환;장병귀;상병돈;이상진;한만희;박창식;이규승
    • Korean Journal of Poultry Science
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    • v.30 no.2
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    • pp.129-134
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    • 2003
  • This study was conducted to investigate the effects of liquid rooster semen on reproductive ability in chicken. Raw and diluted semens were stored at 5$^{\circ}C$ cold temperature for 6, 30, and 54 hours after semen collection. There was no statistically difference in sperm motility throughout the 6 hours period of storage among raw semen and diluted semen groups with skim milk glucose solution (SM), egg yolk glucose solution (EY), and saline. But there was decrease in those throughout the period of 30 and 54 hours of storage. Sperm motility and normal sperm for the period of 30 and 54 hours of storage were significantly better in SM and EY diluted groups (P<0.05). Fertilization rates of rooster semen diluted with SM were 90.77, 87.70, and 59.46% for 6, 30, and 54 hours stored groups, respectively, those proved to be higher in SM-diluted group than other groups.

Traditional Food Use of Frequency of Gwangju City and Chollanamdo Area - In food everyday - (광주와 전라남도의 음식문화 연구 (I) - 일상식 -)

  • 김경애;정난희;전은례
    • Korean Journal of Human Ecology
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    • v.5 no.2
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    • pp.9-21
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    • 2002
  • This study was investigated traditional food utilization actual conditions of Gwangju and Chollanamdo. Frequency of main meal ice plain white rice, boiled rice and cereals, bean-mixed rice, gruel Dakjuk, winter squash porridge, sesame porridge, noodles by noodles cut out with a kitchen knife, noodles with assorted mixtures, soup with dough flakes order frequency much have. Soup ate much beanpaste soup, soup cooked with dried radish leaves, seaweed soup, broth by power-pot soup, hot shredded beef soup, loach soup order. Pot stew soybean paste stew and kimchi stew, beef casserole bean curd beef casserole and small octopus beef casserole often eat. Kimchi ate much cabbage kimchi, radish kimchi, radish cube kimchi, dish of dried slices of radish by sesame leaf dish of dried slices of radish, pickled garlics, Maneuljjong dish of dried slices of radish order. Salted sea foods that eat often were salted anchovies, tiny salted shrimps, Gejang order, and soy sauce were toenjang, korean hot pepper paste, bean-paste soup prepared with around fermented soy beans order, and laver fried kelp, tangle fried kelp, green perilla leaf fried kelp order to fried kelp, and it was bean sprouts, bracken herbs, fragrant edible wild aster herbs order to herbs. It is Ssukgatmuchim, squid debt saliva, Jabanmuchim's order that season, hard-boiled food is beef boiled in soy sauce, mackerel radish hard-boiled food, order of bean curd hard-boiled food, panbroiling ate often by order of Kimchi panbroiling, red pepper anchovy panbroiling, pork panbroiling. Steamed dish is egg steamed dish, fish steamed dish, steamed short-ribs order, fried fish egg speech, by Gimchijeon, Pajeon order, meat roasted with seasoning ate often by laver meat roasted with seasoning, hair-tail meat roasted with seasoning, mackerel meat roasted with seasoning order. Minced raw meat are small octopus raw that live, beef dish of minced raw beef, Hongeohoe order, rice cake is cake made from g1u1ions rice, Seolgitteok, songpyon order, dessert ate often by fermented rice Punch, cinnamon flavored persimmon punch, Kangjung order.

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In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages

  • Lee, Jae Hoon;Ahn, Dong Uk;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.38 no.6
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    • pp.1226-1236
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    • 2018
  • Ovotransferrin (OTF) is a well-known protein of the transferrin family with strong iron chelating activity, resulting in its antimicrobial activity. Furthermore, OTF is known to have antioxidant, anticancer, and antihypertensive activities. However, there have been few studies about the immune-enhancing activity of OTF. In current study, we investigated the immune-enhancing activity of OTF using the murine macrophage cells in vitro. The effect of OTF on production of pro-inflammatory mediators and cytokines were determined using Griess assay and quantitative real-time PCR. Using Neutral Red uptake assay, we confirmed the effect of OTF on phagocytic activity of macrophages. Ovotransferrin significantly increased the production of nitric oxide (NO) and secretion of inducible nitric oxide synthase (iNOS) mRNA with no cytotoxic activity. Ovotransferrin (2 mg/mL) stimulated NO production up to $31.9{\pm}3.5{\mu}M$. Ovotransferrin significantly increased the mRNA expression levels of pro-inflammatory cytokines which are tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), Interleukin-$1{\beta}$ (IL-$1{\beta}$), and IL-6: OTF (2 mg/mL) treatment increased the secretion of mRNA for TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 by 22.20-, 37.91-, and 6.17-fold of the negative control, respectively. The phagocytic activity of macrophages was also increased by OTF treatment significantly compared with negative control. Also, OTF treatment increased phosphorylation level of MAPK signaling pathways. These results indicated that OTF has immune-enhancing activity by activating RAW 264.7 macrophages via MAPK pathways.

The Infection Status and Perception toward Intestinal Trematodes in an Island Inhabitants (일부 도서지역 주민의 장흡충 감염실태 및 인식)

  • Park, Eun-Mi;Kim, Suk-Il;Park, Hyang;Kim, Ki-Soon;Ryu, So-Yeou;Park, Jong
    • Journal of agricultural medicine and community health
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    • v.25 no.2
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    • pp.303-314
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    • 2000
  • In order to evaluate prevalence and perception on the intestinal trematodes of inhabitants at Docho-island, Shinan-gun, Chollanam-do, a questionnaire survey for the epidemiological study and stool examination for the detection of the eggs of the trematodes were done on 224 individuals at the island from January to February, 2000. The results were as follows: 1. Egg positive rate of the intestinal trematodes was 21.0%. 2. The egg positive rate has revealed significantly higher in the elderly over age 70 than other ages, higher in seashore villagers than inland ones, and higher in persons who had a rural and/or marine ph than persons with other jobs, respectively(P<0.05). 3. The egg positive rate of alcoholic drinkers was higher than non-alcoholic persons, and the rate was also higher in smokers than non-smokers. It was statistically significant(P<0.05). 4. The egg positive rate was shown to be significantly higher in inhabitants who ate raw mullet that was well known to be the source of infection with the intestinal trematodes than people who did not eat the raw marine fishes(P<0.01). 5. The egg positive rate was also significantly higher in humans who had the complaints of frequent abdominal pain and diarrhea than healthy people(P<0.05). In conclusion, the prevalence of intestinal trematodes at the island located in the south and west area in Korea was too high to eradicate the parasitic infections. So a special program is necessary for the control of the trematode infections in many adjacent islands at Chollanamdo.

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Anaphylactic Shock in a Breast Milk-Fed Infant due to Skin Contact with Egg White (모유 수유아에서 발생한 계란 흰자 피부접촉에 의한 아나필락시스 쇽 1례)

  • Kim, Eo Jin;Yoon, Young Ran;Yeom, Jung Sook;Kim, Jum Su;Seo, Ji Hyun;Lim, Jae Young;Choi, Myoung Bum;Park, Chan Hoo;Woo, Hyang Ok;Youn, Hee Shang
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.7 no.1
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    • pp.83-86
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    • 2004
  • Food allergy is not uncommon among small children. Cow milk and eggs are most frequently incriminated as the major cause of food allergy. A 4-month-old female infant who did not have a previous history of contact with the egg developed anaphylactic shock when an emulsion of raw egg white was rubbed on the buttock by her mother to relieve erosive diaper dermatitis. She had been fed on breast milk. She had no past medical history of any other allergy and no family history of atopy, asthma or allergic rhinitis. Her IgE PRIST was 29.46 IU/ml and multiple antigen simultaneous testing chemiluminescent assay for food specific IgE antibody showed a level 4 positive value only to egg white.

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Quantitative Analysis of Biogenic Amines in Raw and Processed Foods of Animal Origin on Korean Domestic Market

  • Min, Joong-Seok;Lee, Sang-Ok;Jang, Aera;Lee, Mooha;Kim, Yangha
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.12
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    • pp.1764-1768
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    • 2004
  • This study was performed to examine the levels of biogenic amines (BAs) in foods of animal origin such as egg, ham, sausage, milk, cheese and yoghurt distributed on Korean domestic markets, and to compare the results with those of western countries. Egg yolk had more BAs than egg white. BAs detected in ham samples were the highest level in barbecued tender loin ham. Sausage samples had less BAs than ham samples. However, the delicatessen sausages had significantly higher levels of histamine than the other sausage samples (p<0.001). Cadaverine, spermidine and spermine were detected in market milks and their concentrations were very low compared with other samples. In Cheddar cheese, tyramine (44.46${\pm}$0.83 ${\mu}$g/g) was the major BA. The major BA of stirred yoghurt samples was histamine. These results suggest that BAs of the foods of animal origin distributed on Korean domestic markets were not much different from those of western countries and would not cause any harmful effect to consumers.

Inhibitory Effects of Chicken Egg Yolk Antibody on Infection of Escherichia coli in Macrophage

  • Lee, Jin-Ju;Kim, Dong-Hyeok;Lim, Jeong-Ju;Kim, Dae-Geun;Kim, Gon-Sup;Min, Won-Gi;Lee, Hu-Jang;Rhee, Man-Hee;Chang, Hong-Hee;Kim, Suk
    • Journal of agriculture & life science
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    • v.46 no.2
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    • pp.107-114
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    • 2012
  • The present study evaluated the potential use of immunoglobulin prepared from egg yolk of chickens immunized with Escherichia coli K88 (IgY-Ec) in the control of E. coli K88 infection in RAW 264.7 murine macrophage. The binding activity of IgY-Ec against E. coli K88 surface protein was more specific and increased than control IgY. In infection assay of E. coli in macrophage, the specific IgY-Ec to E. coli K88 remarkably inhibited the phagocytic activity comparing to nonspecific IgY (p<0.001). In adherence assay, bacterial adhesion on macrophage cells was definitely reduced by preincubation of IgY-Ec compared with nonspecific IgY (p<0.05). These findings suggested that IgY-Ec have the protective effects against pathogens and IgY-based diets may have potential benefits for preventing or treating various infections in domestic animals.

Studies on the Utilization of Bivoltine Foundation Cross Males in the Preparation of Cross Breed Eggs of the Silkworm, Bombyx mori L.

  • Rajanna, K.L.;Reddy, G. Vemananda
    • International Journal of Industrial Entomology and Biomaterials
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    • v.25 no.2
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    • pp.171-174
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    • 2012
  • In order to study the feasibility of utilizing bivoltine foundation cross (FC2) males of the silkworm as male components with Pure Mysore (PM), six foundation crosses viz,CSR2 x CSR27, CSR27 x CSR2, CSR27 x CSR50, CSR50 x CSR27, CSR2 x CSR50 and CSR50 x CSR2 along with CSR2 pure breed were assessed for performance of parental crop,grainage, rearing and reeling performance. The foundation crosses exhibited better performance than CSR2 as a parental crop and male component which resulted in higher egg recovery. Though there was not much difference between PM crossed with FC2 and CSR2 males pertaining to important bioassay parameters, PM x CSR2 exhibited superiority in reeling traits particularly with reelability and raw silk %. This study reveals that FC2 seed cocoons can be utilized during exigency when there is a dearth for CSR2 seed cocoons but not regularly.

The Prevalence of Clonorchis sinensis and Its Related Factors at Goksung Area in the Basin of Sumjin River (섬진강유역(蟾津江流域) 곡성지역(谷城地域) 간흡충(肝吸蟲) 감염실태(感染實態) 및 관련요인(關聯要因))

  • Hwang, Min-Hong;Kim, Suk-Il;Park, Jong;Ryu, So-Yeon;Lee, Chul-Gab;Ahn, Hyun-Ok;Kim, Yang-Ok;Kim, Ki-Soon
    • Journal of agricultural medicine and community health
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    • v.22 no.2
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    • pp.239-252
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    • 1997
  • A questionnaire survey including stool examination for the parasite ova, skin test, and enzyme-linked immunosorbent assay (ELISA) of the liver fluke to find the factors related with the prevalence of the parasite infestation was done toward 118 residents living in three villages of Song-jung, Yi-jung, and Chim-gok of Okok-myun, Goksung County, South Cholla Province. The results are as follows : 1. The prevalence of Clonorchis sinensis ova by the stool examination was 46.6%. The sensitivity and specificity of skin test to egg-positive cases were 63.5% and 68.9%. The sensitivity and specificity of ELISA to egg-positive clonorchiasis were 100% and 34.9%. 2. The prevalence of Clonchiasis was higher in male than in female. 45~64 year old group was the highest among age groups, and the longer the duration of living in the present residence was, the higher the prevalence was shown statistically significantly (p<0.01). 3. The present drinker had higher prevalence of Clonorchiasis significantly than the past drinker or non-drinker, and the more the frequency of drinking the higher the prevalence was shown significantly (p<0.05). 4. Those who took raw fish had significantly higher than persons who didn't take it or take cooked fish(p<0.05) and prevalence showed the tendency to be increased as the frequency to take raw fishes increased significantly(p<0.05). 5. Those who had taken a raw sweet fish(Plecoglossus altivelis) or snake-headed fish(Ophicephalus argus) showed significantly higher prevalence of infection with liver fluke than persons who didn't take the raw fish. 6. While 56.4% of persons infected with Clonorchiasis answered that they were healthy, only 13.0% of the subjects were careful for their health, and 41.8% experienced tests to diagnose liver flukes before they were examined by this survey. 7. While 56.4% of infected persons thought the prevalence was high in their community, 54.0% were afraid to be infected, 47.1% thought the infection might destroy their health, 25.5% took treatment drugs, and 18.2% took raw fish continuously after treatment. The above results confirmed that Goksung area was highly prevalent in Clonorchiasis, so it was necessary to push effective preventive program against liver fluke infection together with the program to diagnose and treat existing infected persons.

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