• Title/Summary/Keyword: rapid preparation

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Preparation of Monodispersed Titania from TPOT (TPOT로부터 단분산 TiO2분말 합성)

  • 안영필;최석홍
    • Journal of the Korean Ceramic Society
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    • v.25 no.6
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    • pp.677-684
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    • 1988
  • Titania powders were obtained from the various hydrolysis condition the variation of Alkoxide, water/Alcohol, REaction, Temp., reaction time and solvent system were investigated. In this result, spherical monodisperesed titania gel powders(≒0.7${\mu}{\textrm}{m}$) were obtained using EtOH(as solvent), and this method had rapid reaction time compare with iso-PrOH(as solvent).

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Development of simultaneous determination of vitamin A and E in infant formula by micro-HPLC (Micro-HPLC를 이용한 조제분유 중 비타민 A.E 동시분석법 개발)

  • Yun I-Ran;Choi You-Jeong;Lee Min-Kwon;Jeong Myeong-Ho;Kim Byeong-Hun
    • Korean Journal of Veterinary Service
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    • v.29 no.3
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    • pp.339-346
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    • 2006
  • Semi-micro-HPLC using a column-switching technique was developed for simultaneous determination of vitamin A and E contents in infant formula. Vitamin A and E were extracted by PDA - HPLC with reversed phase column using organic solvent and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with hydrolysis method and rapid extraction. Developed method has many advantages of simple and rapid sample preparation and simultaneous determination of vitamin A and E by micro-HPLC using reversed phase column.

A Study on Rapid Prototyping using VRML Model (VRML 모델을 이용한 쾌속조형에 관한 연구)

  • 김호찬;이주호;반갑수;최홍태;이석희
    • Journal of the Korean Society for Precision Engineering
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    • v.17 no.7
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    • pp.63-73
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    • 2000
  • Internet becomes very common tool for communication and data sharing. Virtual reality(VR) on web browser, and virtual prototyping and virtual manufacturing is widely used in many engineering folds. The reduction of overall development process and error minimization during data conversion becomes very crucial where sharing data via Internet and VR. This paper deals with the advantage and disadvantage of VRML format used in RP(Rapid Prototyping), and a software for RP data preparation is developed. If VRML format as an international standard for VR, is replaced with STL format, the weak points of STL format can be overcome and the technique related to virtual prototyping and virtual manufacturing can be addressed more systematically by sharing the data. The system developed in this work shows a good window to get access to a more realistic observation of an object fur an RP system from a long remote sites in a more systematic way.

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Improvement of the pre-hospital emergency medical service system in China (중국의 병원 전 응급의료체계 개선방안)

  • Wang, Chengying;Choi, Eun-Sook
    • The Korean Journal of Emergency Medical Services
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    • v.16 no.2
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    • pp.53-66
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    • 2012
  • Purpose : Emergency medical services in China are increase in demand by people and under the greater pressure than ever before. So it is, necessary to advance the pre-hospital system in order to promote the development of emergency medical services. Methods : This is based on China-related articles, books, journals, reports, statistical data and other literature. Results : First, pre-hospital emergency medical care with the introduction of specialist training program should be established. Second, to strengthen pre-hospital emergency services and to develop the EMS guidelines. Third, the "120" reporting systems unification and awareness activation. Fourth, the preparation of the EMS facilities equipment system. Fifth, the rapid transport system establishment to the selected medical institutions. Conclusion : It is necessary to strengthen the emergency medical personnel at the scene, rapid transport, rapid patient triage and to improve the survival rate of the patients.

Serological Diagnosis of Bordetellosis: Application of Rapid Plate Agglutination Technique for the Detection of Carrier in Swine (Bordetella 감염증(感染症)의 혈청학적진단(血淸學的診斷): 특히 보균돈검색(保菌豚檢索)을 위한 급속평판응집반응(急速平板凝集反應)의 실용화(實用化))

  • Kang, Byong Kyu
    • Korean Journal of Veterinary Research
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    • v.18 no.2
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    • pp.61-67
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    • 1978
  • The detection of Bordetella bronchiseptica which is supposed to be an agent of the infectious atrophic rhinitis of swine, is likely to receive more attention in the future as the pork industry comes to realize that eradication of this infection from breeding herds is a practical possibility. Experiments described here were carried out to establish the rapid plate agglutination test for the detection of the infectious atrophic rhinitis of swine in the field using the criteria of antigen preparation, effects on the antigenecity after storing of the antigen and reaction appearing time. Also, the agglutinabilities between the plate and tube method were compared and the degree of pathological lesions were recorded in relation to tube agglutination titers. Obtained results were as follows: 1. No differences were noted in the agglutinabilities on the plate agglutination test between the treatments in antigen preparation-formolized, merthiolate-killed and living organism. 2. The agglutinability of the antigens did not show any significant changes until 10 weeks of storage at 4 C; however, after 10 weeks of storage, non-specific reaction was observed with the HPCD control sera. 3. The results of the plate and tube agglutination tests were not comparable but the effective use of the plate method in Bordetella bronchiseptica eradication programs in pigs especially in the sow is stressed as a screening test.

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Preparation process of functional particles: III. Preparation of composite particles by rapid expansion of supercritical fluid solutions and release behavior (기능성 미분말의 제조공정에 관한 연구 : III. 초임계 분출법에 의한 복합분체의 합성과 용출특성)

  • ;;;Eiichi Abe
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.1
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    • pp.55-59
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    • 1999
  • The Rapid Expansion of Supercritical fluid Solutions (RESS) process was applied to particles coating. Microcapsules prepared by spray drying were used as the core particles, and two kinds of paraffin were used as the coating materials. Supercritical $CO_{2}$ solutions of paraffin were expanded through the short nozzle into the bed that was fluidized by air. Extraction temperature and pressure were varied at $50~120^{\circ}C$, $150~200\;kg/\textrm{cm}^2$, respectively. The thickness of theoritical coating layer ws measured, and precipitate coating layer on surface was analyzed by using SEM, FT-IR. The release behaviors of $Mg^{2+}$ ions were inspected by atomic absorbance spectrophtometer.

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DNA Microarray Probe Preparation by Gel Isolation Nested PCR

  • Wang, Hong-Min;Ma, Wen-li;Huang, Hai;Xiao, Wei-Wei;Wang, Yan;Zheng, Wen-Ling
    • BMB Reports
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    • v.37 no.3
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    • pp.356-361
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    • 2004
  • To develop a simplified method that can rapidly prepare DNA microarray probes in a massive scale, a lambda phage genomic DNA-fragments library was constructed for the microarray-probes collection. Four methods of DNA band recovery from the first PCR products were tested and compared. The DNA microarray probes were collected by a novel method of nested PCR that was mediated by gel isolation of the first PCR products. This method was named GIN-PCR. The probes that were prepared by this GIN-PCR technique were used as subjects to fabricate a DNA microarray. The results showed that a wooden toothpick was superior to the other 3 methods, since this technique can steadily transfer the DNA bands as the template of the second PCR after the first PCR. A group of probes were successfully collected and DNA microarrays were constructed using these probes. Hybridization results demonstrated that this technique of DNA recovery and probe preparation was rapid, efficient, and effective. We developed a cost-effective and less labor-intensive method for DNA microarray probe preparation by nested PCR that is mediated by wooden toothpick transfer of the DNA bands in the gel after electrophoresis.

Sample Preparation and Nucleic Acid-based Technologies for the Detection of Foodborne Pathogens (식중독균의 검출을 위한 시료전처리 및 핵산기반의 분석기술)

  • Lim, Min-Cheol;Kim, Young-Rok
    • Food Engineering Progress
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    • v.21 no.3
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    • pp.191-200
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    • 2017
  • There have been great efforts to develop a rapid and sensitive detection method to monitor the presence of pathogenic bacteria in food. While a number of methods have been reported for bacterial detection with a detection limit to a single digit, most of them are suitable only for the bacteria in pure culture or buffered solution. On the other hand, foods are composed of highly complicated matrices containing carbohydrate, fat, protein, fibers, and many other components whose composition varies from one food to the other. Furthermore, many components in food interfere with the downstream detection process, which significantly affect the sensitivity and selectivity of the detection. Therefore, isolating and concentrating the target pathogenic bacteria from food matrices are of importance to enhance the detection power of the system. The present review provides an introduction to the representative sample preparation strategies to isolate target pathogenic bacteria from food sample. We further describe the nucleic acid-based detection methods, such as PCR, real-time PCR, NASBA, RCA, LCR, and LAMP. Nucleic acid-based methods are by far the most sensitive and effective for the detection of a low number of target pathogens whose performance is greatly improved by combining with the sample preparation methods.

Surface Preparation and Activation Only by Abrasion and Its Effect on Adhesion Strength

  • Ali Gursel;Salih Yildiz
    • Journal of Adhesion and Interface
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    • v.23 no.4
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    • pp.101-107
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    • 2022
  • Adhesive joints have many advantages such as weight savings, corrosion and fatigue resistance and now developed even withstand of high impact and dynamic loads. However, an adhesion has cumbersome and complicated surface preparation processes. The surface preparation step is critical in adhesive joint manufacturing in order to obtain the prescribed strength for adhesive joints. In this study, it was attempted to simplify and reduce the number of surface preparation steps, and abrasion and rapid adhesive application (ARAA) process is developed for an alternative solution. The abrasion processes are performed only for creating surface roughness in standard procedures (SP), although the abrasion processes cause surface activation itself. The results showed that there is no need the long procedures in laboratory or chemical agents for adhesion. After the abrasion process, the attracted and highly reactive fresh surface layer obtained, and its effect on bonding success is observed and analyzed in this research, in light of the essential physic and adhesion theories. Al 6061 aluminum adherends and epoxy-based adhesives were chosen for bonding processes, which is mostly used in light vehicle parts. The adherends were cleaned, treated and activated only with abrasion, and after the adhesive application the specimens were tested under quasi-static loading. The satisfied ARAA results were compared with that of the specimens fabricated by the standard procedure (SP) of adhesion processes of high impact loads.

Natech Risk Assessment of Chemical Facilities in the Event of Earthquake in Korea using RAPID-N (RAPID-N을 이용한 국내 지진 발생 시 화학시설 Natech 위험성 평가)

  • Park, Jaehyuk;Yeon, Eungjin;Lee, Hak Tae;Jung, Seungho
    • Journal of the Korean Society of Safety
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    • v.34 no.4
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    • pp.111-118
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    • 2019
  • Accidents occurring due to natural disasters in chemical process facilities where technologies are concentrated can cause secondary damage. The concept of the relationship between natural disasters and highly intensive technologies has evolved into the Natech (Natural Hazards Triggered Technological Disaster) research. Currently, the number of earthquakes is increasing all over the Korean peninsula. To assess the risk of Natech when an earthquake has occurred in South Korea, the Rapid Natech Risk Assessment Tool (RAPID-N) developed by the European Commission's Joint Research Center (EC JRC) was used in the present study. The RAPID-N can be used for Natech risk assessment based on mapping and can be utilized for sufficient preparation for reduction of the effects of Natech accidents. A total of 261 chemical facilities actually existing in Pohang were initially analyzed to select eight facilities and the Pohang earthquake that occurred in 2017 was implemented in the RAPID-N utilizing the shake map. High risk areas were selected through Natech risk assessments for the selected eight work places and countermeasures for the areas were suggested. High risk areas exist depending on the location, since the damage influence ranges could be overlapped and each chemical facility has an independent probability of Natech. Therefore, studies on Natech emergency plans and emergency evacuation routes should be actively conducted considering such high risk areas. The present study was conducted to demonstrate the feasibility of Natech risk assessment in South Korea through the RAPID-N. These findings can be used as a reference material to lay a foundation for Natech risk assessment and related policies in South Korea.