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Development of simultaneous determination of vitamin A and E in infant formula by micro-HPLC  

Yun I-Ran (Gyeongnam Livestock Promotion Research Institute)
Choi You-Jeong (Gyeongnam Livestock Promotion Research Institute)
Lee Min-Kwon (Gyeongnam Livestock Promotion Research Institute)
Jeong Myeong-Ho (Gyeongnam Livestock Promotion Research Institute)
Kim Byeong-Hun (Gyeongnam Livestock Promotion Research Institute)
Publication Information
Korean Journal of Veterinary Service / v.29, no.3, 2006 , pp. 339-346 More about this Journal
Abstract
Semi-micro-HPLC using a column-switching technique was developed for simultaneous determination of vitamin A and E contents in infant formula. Vitamin A and E were extracted by PDA - HPLC with reversed phase column using organic solvent and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with hydrolysis method and rapid extraction. Developed method has many advantages of simple and rapid sample preparation and simultaneous determination of vitamin A and E by micro-HPLC using reversed phase column.
Keywords
Rapid extraction; Micro-HPLC; Simultaneous determination; Vitamin A and E;
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