• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
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• pp.402-407
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• 2002

Laminate tooling process is a fast and simple method to make metal tools directly for various molding processes such as injection molding in rapid prototyping field. Metal sheets are usually cut, stacked, aligned and joined with brazing or soldering. Through the joining process, all of the metal sheet layers should be rigidly joined. When joining process parameters are not appropriate, there would be defects in the layers. Among various types of defects, non-bonded gaps of the tool surface are of great importance, because they directly affect the surface quality and dimensional accuracy of the final products. If a laminate tool with defects has to be abandoned, it could lead to great loss of time and cost. Therefore a repair method for non-bonded gaps of the surface is essential and has important meaning for rapid prototyping. In this study, a rapid laminate tooling system composed of a CO2 laser, a furnace, and a milling machine was developed. Metal sheets were joined by furnace brazing, dip soldering and adhesive bonding. Joined laminate tools were machined by a high-speed milling machine to improve surface quality. Also, repair brazing and soldering methods of the laminates using the $CO_2$ laser system have been investigated. ill laser repair process, the beam duration, beam power and beam profile were of great importance, and their effects were simulated by [mite element methods. The simulation results were compared with the experimental ones, and optimal parameters for laser repair process were investigated.

• 대한치과교정학회지
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• 제49권3호
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• pp.150-160
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• 2019

Objective: This study aimed to analyze the effect of changing various parameters of the bone-borne rapid palatal expander (RPE) using the finite element method (FEM). Methods: In eight experimental groups, we investigated the effect of the number, position, and length of miniscrews; positional changes of the expander; and changes in the hook length on maxillary expansion. In finite element analysis, we compared the magnitude and distribution of stress, and the displacement changes following expansion of the bone-borne RPE. Results: When we compared the number and position of miniscrews, placing miniscrews in the anterior and posterior sides was advantageous for maxillary expansion in terms of stress distribution and displacement changes. Miniscrew length did not significantly affect stress distribution and displacement changes. Furthermore, anteroposterior displacement of the expander did not significantly affect transverse maxillary expansion but had various effects on vertical changes of the maxilla. The maxilla rotated clockwise when the miniscrews were placed in the anterior region. The hook length of the expander did not show consistent results in terms of changes in stress distribution and magnitude or in displacement changes. Conclusions: The findings of this study suggest that changes in the location and length of the miniscrews and displacement of the bone-borne RPE could affect the pattern of the maxillary expansion, depending on the combination of these factors.

• 대한건축학회논문집:구조계
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• 제34권6호
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• pp.29-34
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• 2018

In this research, regarding the cement supplied to ready mixed concrete plant, the feasibility of rapid acquisition test for cement fineness using hydrometer which used for early strength evaluation of concrete was analyzed. Additionally, regarding the rapid test with hydrometer control factors were provided. As a result of analysis, quality control using hydrometer was possible with the regressive equation obtained in five minute between density of suspension and fineness of cement powder. As the control factors, dispersing admixture, replacing kerosene as a medium, and temperature of cement and water were evaluated. According to the control factor evaluation, the tap water was optimum as a medium and calibration of keeping the temperature of water to $20^{\circ}C$ or correction factor was needed for density results. Finally, it is considered that the suggested rapid quality evaluation method using hydrometer is cheaper and easier method than currently used Blaine test.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권12호
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• pp.4776-4794
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• 2020

We propose a rapid adaptive learning framework for streaming object detection, called EER-ASSL. The method combines the expected error reduction (EER) dependent rollback learning and the active semi-supervised learning (ASSL) for a rapid adaptive CNN detector. Most CNN object detectors are built on the assumption of static data distribution. However, images are often noisy and biased, and the data distribution is imbalanced in a real world environment. The proposed method consists of collaborative sampling and EER-ASSL. The EER-ASSL utilizes the active learning (AL) and rollback based semi-supervised learning (SSL). The AL allows us to select more informative and representative samples measuring uncertainty and diversity. The SSL divides the selected streaming image samples into the bins and each bin repeatedly transfers the discriminative knowledge of the EER and CNN models to the next bin until convergence and incorporation with the EER rollback learning algorithm is achieved. The EER models provide a rapid short-term myopic adaptation and the CNN models an incremental long-term performance improvement. EER-ASSL can overcome noisy and biased labels in varying data distribution. Extensive experiments shows that EER-ASSL obtained 70.9 mAP compared to state-of-the-art technology such as Faster RCNN, SSD300, and YOLOv2.

• The Plant Pathology Journal
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• 제38권2호
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• pp.159-166
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• 2022

Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42℃ for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.

• Journal of Veterinary Science
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• 제21권1호
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• pp.4.1-4.9
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• 2020

Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.

• 한국어병학회지
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• 제28권2호
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• pp.71-78
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• 2015

In this study, we conducted to the development of a rapid antimicrobial susceptibility test method using WST-1 which is known to water-soluble tetrazolium salt, in order to rapidly response against bacterial diseases in fish. Eight of antibiotics which are permissioned for marine organism from government were used to rapid antimicrobial susceptibility testing using the WST-1. As a result, a similar tendency was verified compare to conventional antibiotic susceptibility test results. Generally, the antibiotic susceptibility test method required about 3 days (72 hours) for determine the effective antibiotics, however, we have confirmed that the our method using WST-1 was required at least 36 hours in this study. Consequentially, our method will contribute to development of rapid antimicrobial susceptibility testing for bacterial diseases in fish.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.1-6
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• 2013

Tetanus is a specific infectious disease, which is often associated with catastrophic events such as earthquakes, traumas, and war wounds. The obligate anaerobe Clostridium tetani is the pathogen that causes tetanus. Once the infection of tetanus progresses to an advanced stage within the wounds of limbs, the rates of amputation and mortality increase manifold. Therefore, it is necessary to devise a rapid and sensitive point-of-care detection method for C. tetani so as to ensure an early diagnosis and clinical treatment of tetanus. In this study, we developed a detection method for C. tetani using loop-mediated isothermal amplification (LAMP) assay, wherein the C. tetani tetanus toxin gene was used as the target gene. The method was highly specific and sensitive, with a detection limit of 10 colony forming units (CFU)/ml, and allowed quantitative analysis. While detecting C. tetani in clinical samples, it was found that the LAMP results completely agreed with those of the traditional API 20A anaerobic bacteria identification test. As compared with the traditional API test and PCR assay, LAMP detection of C. tetani is simple and rapid, and the results can be identified through naked-eye observation. Therefore, it is an ideal and rapid point-of-care testing method for tetanus.

• Journal of Biosystems Engineering
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• 제38권2호
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• pp.121-128
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• 2013

Purpose: Porcine proliferative enteropathy (PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. In order to diagnose PPE rapidly, the rapid kit was developed and tested. Methods: In this study, a rapid kit was developed to screen the PPE rapidly at the pig farm. Also, occult blood test with fecal occult blood (FOB) kit was done for detecting the blood in pig feces which might be the evident of hemorrhagic PPE. For developing the kit, we tested fecal samples of PPE infected pigs diagnosed by polymerase chain reaction (PCR) method. Results: With the developed rapid kit, Lawsonia intracellularis was detected in high density emulsion of ileum. On the other hand, the test result of detecting Lawsonia in feces showed too high non-specific response. In addition, nevertheless the FOB test result showed that blood evident could be founded in pig feces, the diagnosing result was not fit to PCR test result, which shows blood in pig feces could be from not only hemorrhagic PPE but also many reasons. Conclusions: To deal with the PPE effectively, it will be better for farmers to screen the PPE in earlier stage with easy and rapid diagnosing tool on farm. This study found out that the rapid kit could detect the Lawsonia intracellularis and hemoglobin in pig feces. However, the non-specific response to negative samples of PPE was too high to use at a pig farm. Further research is needed for lowering the non-specific response with the rapid kit.

• 소성∙가공
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• 제16권7호
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• pp.538-548
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• 2007

In this paper, a novel approach which enables rapid die surface modification for sheet metal forming process is proposed. In this method an implicit surface which interpolates a given set of control points and displacement constraints is generated to compute the displacements at arbitrary points located on die surface. The proposed method does not depend on the underlying surface representation type and is affected neither by its complexity nor by its quality. In addition, the domain decomposition method is introduced in order to treat large surface model. The global domain of interest is divided into smaller domains where the problem can be solved locally. And then the local solutions are combined together to obtain a global solution. In order to verify the validity and effectiveness of the proposed method, various surface modifications are carried out fur three kinds of die surface model including polygonal surface composed of triangular and rectangular meshes, polynomial surface and NURBS surface.