• Bulletin of the Korean Chemical Society
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• v.35 no.4
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• pp.1082-1086
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• 2014

A multi-channel microchip electrophoresis (MCME) method with parallel laser-induced fluorescence (LIF) detection was developed for rapid screening of H1N1 virus. The hemagglutinin (HA) and nucleocapsid protein (NP) gene of H1N1 virus were amplified using polymerase chain reaction (PCR). The amplified PCR products of the H1N1 virus DNA (HA, 116 bp and NP, 195 bp) were simultaneously detected within 25 s in three parallel channels using an expanded laser beam and a charge-coupled device camera. The parallel separations were demonstrated using a sieving gel matrix of 0.3% poly(ethylene oxide) ($M_r$ = 8,000,000) in $1{\times}$ TBE buffer (pH 8.4) with a programmed step electric field strength (PSEFS). The method was ~20 times faster than conventional slab gel electrophoresis, without any loss of resolving power or reproducibility. The proposed MCME/PSEFS assay technique provides a simple and accurate method for fast high-throughput screening of infectious virus DNA molecules under 400 bp.

• Journal of Internet Computing and Services
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• v.3 no.5
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• pp.31-39
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• 2002

A rapid growth of internet and sudden increase of multimedia data demands for high-speed transfer media and if optimizec usage from the interface system. To achieve this level of network bandwidth, multiple NICs for support of high-speed network bandwidth have been developed and studied. Furthermore, the use of multiple NICs can provide high-speed LAN environment without large network environment modification, supports backward compatibility of current system and reduce overhead. However. if system failure is caused by SPOF(Single Point of Failure) fault of large-capacity multiple NICs, incredible loss will be met because it services large capacity of multimedia data, Therefore, to prevent loss coming from faults, we describe 'Fault tolerance of multiple NICs', which use the fault prevention mechanism. Considering inefficiency of availability and serviceability that is occurred with existing TMR, Primary-Standby approach and Watchdog time mechanism, we propose and design the efficient fault tolerance mechanism, which minimize down time as changing of detection period dynamically. Consequently, the fault tolerance mechanism proposed for reducing overhead time when the fault is occurred, should minimize system downtime overall.

• Journal of The Korean Society of Clinical Toxicology
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• v.2 no.2
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• pp.147-150
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• 2004

Hydrogen sulfide is a colorless, and malodorous 'rotten eggs' gas that results from the decay of organic material. It is a byproduct of industry and agriculture. The mechanism of its toxicity is primarily related to inhibition of oxidative phosphorylation, which causes a decrease in available cellular energy. Because there is no rapid method of detection that is of clinical diagnostic use, management decisions must be made based on history, clinical presentation, and diagnostic tests that imply hydrogen sulfide's presence. Although there is some anecdotal evidence to suggest that the early use of hyperbaric oxygen is beneficial, supportive care remains the mainstay of therapy. We describe an occupational exposure to hydrogen sulfide gas in 51-year-old man. While cleaning the sewage of pigs. he became unconscious. When he arrived in the emergency department, he had irritability and confused mentality. The typical smell of rotten eggs on clothing and exhaled air were enough to be considered to be exposed to hydrogen sulfide. Hyperbaric oxygen therapy was performed. He had a recovery to normal function.

• Food Science of Animal Resources
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• v.42 no.4
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• pp.655-671
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• 2022

There are differences of spectral characteristics between different types of meat cut, which means the model established using only one type of meat cut for meat quality prediction is not suitable for other meat cut types. A novel portable visible and near-infrared (Vis/NIR) optical system was used to simultaneously predict multiple quality indicators for different commercial meat cut types (silverside, back strap, oyster, fillet, thick flank, and tenderloin) from Small-tailed Han sheep. The correlation coefficients of the calibration set (R_c) and prediction set (R_p) of the optimal prediction models were 0.82 and 0.81 for pH, 0.88 and 0.84 for L^*, 0.83 and 0.78 for a^*, 0.83 and 0.82 for b^*, 0.94 and 0.86 for cooking loss, 0.90 and 0.88 for shear force, 0.84 and 0.83 for protein, 0.93 and 0.83 for fat, 0.92 and 0.87 for moisture contents, respectively. This study demonstrates that Vis/NIR spectroscopy is a promising tool to achieve the predictions of multiple quality parameters for different commercial meat cut types.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.25 no.4
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• pp.434-441
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• 2014

Recently, a mutual interference threat has been increasing among the radar systems due to the rapid growth of the military radar operation. In this paper, the radar interference protection criteria is presented for interoperability in terms of the radar coverage and target detection probability in association with the international recommendation on the interference spectrum by ITU-R. The required criteria for the minimum allowable interference is also presented in terms of INR. In order to ensure the maximum detection probability of the radar under the mutual interference situation, only 5 % of detection range loss is allowed for the case of INR of -6 dB, and required SNR is presented at each INR in terms of the detection range and detection probability. This result will be useful for establishing the interference protection criteria in the combined military radar systems.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.22 no.3
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• pp.545-552
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• 2012

The rapid development of information technology is making large changes in our lives today. Also the infrastructure and services are combinding with information technology which predicts another huge change in our environment. However, the development of information technology brings various types of side effects and these side effects not only cause financial loss but also can develop into a nationwide crisis. Therefore, the detection and quick reaction towards these side effects is critical and much research is being done. Intrusion detection systems can be an example of such research. However, intrusion detection systems mostly tend to focus on judging whether particular traffic or files are malicious or not. Also it is difficult for intrusion detection systems to detect newly developed malicious codes. Therefore, this paper proposes a method which determines whether the present network model is normal or abnormal by comparing it with past network situations.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.2
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• pp.692-712
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• 2022

Buoys used for Aid to Navigation systems are widely used to guide the sea paths and are powered by batteries, requiring continuous battery replacement. However, since human labor is required to replace the batteries, humans can be exposed to dangerous situation, including even collision with shipping vessels. In addition, Maritime sensors are installed on the route signs, so that these are often damaged by collisions with small and medium-sized ships, resulting in significant financial loss. In order to prevent these accidents, maritime object detection technology is essential to alert ships approaching buoys. Existing studies apply a number of filters to eliminate noise and to detect objects within the sea image. For this process, most studies directly access the pixels and process the images. However, this approach typically takes a long time to process because of its complexity and the requirements of significant amounts of computational power. In an emergent situation, it is important to alarm the vessel's rapid approach to buoys in real time to avoid collisions between vessels and route signs, therefore minimizing computation and speeding up processes are critical operations. Therefore, we propose Fast Connected Component Labeling (FCCL) which can reduce computation to minimize the processing time of filter applications, while maintaining the detection performance of existing methods. The results show that the detection performance of the FCCL is close to 30 FPS - approximately 2-5 times faster, when compared to the existing methods - while the average throughput is the same as existing methods.

• Journal of Oriental Neuropsychiatry
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• v.16 no.2
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• pp.287-299
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• 2005

Dissociative disorder is a psychiatric disorder characterized by a sudden loss of memory, but which has no organic disease or explanation. It usually occurs after heavy psychosocial stress or traumatic experience. A transient cerebral ischemic attack (TIA) is an acute episode of temporary and focal loss of cerebral function of vascular origin. TIAs are rapid in onset; symptoms reach their maximal manifestation in fewer than 5 minutes. Manifestations are of variable duration and typically last 2-15 minutes(rarely as long as 24 h). Most TIA durations are less than 1 hour. Of concern is the careful detection of changes in behavior, speech, gait, memory, movement, and vision. TIAs are uncommon in persons younger than 60 years. I treat 6 cases of Sudden Temporary Amnesia Patients with oriental medicine and they are improved. All of them had amnesia for $6{\sim}10\;hours$. During that time, they show behavioral changes and they are not on the state of unconsciousness. After recovery, they also forget what happen at the time. they have some emotional reason too. In conclusion, 4 cases of them belong to dissociative disorder and 2 other cases, TIA.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.4
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• pp.263-266
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• 2007

Vibrio vulnificusis a causative agent of serious diseases in humans resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately $84^{\circ}C$ for V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or $10^3$ V. vulnificus cells from pure cultured broth and $10^3$ cells in 1g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium using the rpoS gene in pure cultures and in infected oyster tissues.

• Analytical Science and Technology
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• v.26 no.6
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• pp.375-380
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• 2013

Ammonium biflouride ($NH_4HF_2$) digestion was studied for germanium analysis in rock and sediment by inductively coupled plasma mass spectrometry (ICP/MS). QLO-1 and SDO-1 are used for reference materials from USGS. Sediment, basalt and ball clay for GeoPT were chosen as real samples. The loss of germanium in open vessel digestion was well known which can be caused by easy transformation to volatile compounds. But ammonium bifluoride digestion could suppress loss of germanium in open vessel digestion. Germanium recovery was not influenced by hydrogen peroxide with ammonium bifluoride digestion. Furthermore, the new method was simple and rapid in germanium analysis by ICP/MS. MDL(method detection limit) was 0.015 ${\mu}g/g$ and germanium recovery was 106~128%.