• Journal of Integrative Natural Science
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• v.9 no.3
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• pp.171-176
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• 2016

Rap1 is a key regulator of cell adhesion and migration. Although increasing evidence indicates that the Rap1 signaling pathway is involved in the process of bone remodeling, the mechanism by which Rap1 regulates osteoblastic differentiation and cell adhesion remains unknown. Here, we investigated the morphological characteristics and osteoblastic differentiation of cells expressing constitutively activated form of Rap1A (Rap1ACA) or Rap1 GTPase activating protein Rap1GAP and found that activated Rap1 induces osteoblastic differentiation and cell adhesion as well as cell spreading. When osteoblastic differentiation was induced, Rap1ACA cells showed considerably higher levels of calcium deposits than the wild-type and Rap1GAP-overexpressing cells did. Rap1ACA cells showed increased spreading and size, as well as strong cell adhesion and significantly decreased growth rates. F-actin staining using phalloidin revealed several thin thread-like filopodia around the protrusions in Rap1ACA cells, which possibly contribute to the increased cell adhesion.

• Journal of the Korean Geotechnical Society
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• v.20 no.5
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• pp.127-134
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• 2004

Rammed Aggregate Pier (RAP) has extensive applicability as for a foundation of structures. In this study, bearing capacity of the reinforced ground by RAP and the failure behavior of RAP are investigated through experiments. RAPs with diameters of 45, 60, 70 mm were installed in sand, of which relative densities are 60, 70, 90%. Then, two columns of pressure gauges, near the RAPs and one diameter off from the center of piers, are installed 5, 10, 15, 20, 25, 30 cm from the surface of the ground. The test results show that maximum lateral earth pressure is observed near 5∼10 cm (1.0∼2.0D) from the surface, which indicates the occurrence of bulging failure type. In addition, deformation of RAP in radial direction increases with lower relative density of the ground. Furthermore, lateral stress distribution decreases with depth.

• Structural Engineering and Mechanics
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• v.67 no.3
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• pp.283-290
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• 2018

This paper presents the results of a study that investigated the influence of using recycled coarse aggregate (RCA) and recycled asphalt pavement (RAP) on the properties of pervious concrete (PC). The natural aggregate (NA) was replaced by RCA and RAP in the PC with replacement levels of 0%, 20%, 40%, 60% and 80% by the total weight of NA, respectively. In addition to incorporating RAP and RCA in the same mixes with replacement levels of: (1) 20% RAP and 80% RCA; (2) 60% RAP and 40% RCA; and (3) 80% RAP and 20% RCA. Water permeability, thermal conductivity, density, porosity, void content, and compressive, splitting tensile and flexural strengths were studied in this paper. The results showed that using RCA, RAP, and (RAP-RCA) enhanced the properties of PC in general and improved the mechanical properties significantly in particular. The optimum mix was reported to be the 60% RAP and 40% RCA. Accordingly, the RAP has the potential to be used in PC in order to reduce the negative impact of RAP on the human health and environment.

• Structural Engineering and Mechanics
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• v.62 no.3
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• pp.357-364
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• 2017

This paper presents the results of a study that investigated the improvement of the mechanical properties of coarse and fine recycled asphalt pavement (RAP) produced by adding silica fume (SF) with contents of 5%, 10%, and 15% by total weight of the cement. The coarse and fine natural aggregate (NA) were replaced by RAP with replacement ratio of 20%, 40% and 60% by the total weight of NA. In addition, SF was added to NA concrete mixes as a control for comparison. Twenty eight mixes were produced and tested for compressive, splitting tensile and flexural strength at the age of 28 days. The results show that the mechanical properties decrease with as the content of RAP increases. And the decrease in the compressive strength was more in the fine RAP mixes compared to the coarse RAP mixes, while the decrease in the splitting tensile and flexural strength was almost the same in both mixes. Furthermore, using SF enhances the mechanical properties of RAP mixes where the optimum content of SF was found to be 10%, and the mechanical properties enhancement of coarse RAP were better than fine RAP mixes. Accordingly, the RAP has the potential to be used in the concrete pavements or in other low strength construction applications in order to reduce the negative impact of RAP on the environment and human health.

• Advances in concrete construction
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• v.10 no.6
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• pp.489-498
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• 2020

This paper investigates mechanical properties of roller compacted concrete (RCC) involving reclaimed asphalt pavement (RAP). In this way, a set of 276 cylindrical RCC specimens were prepared with different RAP sizes (i.e., fine, coarse & total) at various ratios (i.e., 10%, 20%, and 40%). Results reveal that incorporation of RAP decreases unconfined compressive strength (UCS), modulus of elasticity (E), and indirect tensile (IDT) strength of RCC. For each RAP size, a regression model was used to maximize RAP content while satisfying the UCS lower limit (27.6 Mpa) mentioned by ACI as a minimum requirement for RCC used in pavement construction. Moreover, UCS of RAP incorporated mixes, dissimilar to that of control mixes, was found to be sensitive and insensitive to the testing temperature and curing time after 7 days, respectively. The results also demonstrate that the higher amounts of RAP, the more flexibility in RCC is. This issue was also proved by the results of modulus of elasticity test. In addition, the toughness index (TI) shows that increase in RAP content leads to up to 43% increase in energy absorbance capacity of RCC.

• Parasites, Hosts and Diseases
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• v.60 no.6
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• pp.393-400
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• 2022

Human infection with simian malaria Plasmodium knowlesi is a cause for concern in Southeast Asian countries, especially in Malaysia. A previous study on Peninsular Malaysia P. knowlesi rhoptry associated protein-1 (PkRAP1) gene has discovered the existence of dimorphism. In this study, genetic analysis of PkRAP1 in a larger number of P. knowlesi samples from Malaysian Borneo was conducted. The PkRAP1 of these P. knowlesi isolates was PCR-amplified and sequenced. The newly obtained PkRAP1 gene sequences (n=34) were combined with those from the previous study (n=26) and analysed for polymorphism and natural selection. Sequence analysis revealed a higher genetic diversity of PkRAP1 compared to the previous study. Exon II of the gene had higher diversity (π=0.0172) than exon I (π=0.0128). The diversity of the total coding region (π=0.0167) was much higher than those of RAP1 orthologues such as PfRAP-1 (π=0.0041) and PvRAP1 (π=0.00088). Z-test results indicated that the gene was under purifying selection. Phylogenetic tree and haplotype network showed distinct clustering of Peninsular Malaysia and Malaysian Borneo PkRAP1 haplotypes. This geographical-based clustering of PkRAP1 haplotypes provides further evidence of the dimorphism of the gene and possible existence of 2 distinct P. knowlesi lineages in Malaysia.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.2
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• pp.116-121
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• 2019

In order to evaluating applicability of RAP (recycled aggregate powder) in mortar, in this study, physical and mechanical tests was carried out. Material characteristics of recycled aggregate and RAP were evaluated and the mechanical properties of mortar replaced with RAP were analyzed. Test result of sieve analysis showed that as the milling time increased the fineness modulus was decreased and the distribution of 0.6 mm particle size was found to increase. The fluidity of mortar mixture substituted with RAP tended to increase than Plain mixture. It was result that the increasing fluidity was affected by unreacted surplus water in the mortar as the binder was replaced with RAP. From the compressive strength result of the mortar subjected to RAP, it was found that the RAP was able to replace up to about 10% of unit binder weight although the compressive strength of mortar was decreased as the RAP replacement increased. From the above study, it can be concluded that the physical properties of RAP satisfied the quality standard of aggregate for replacement with fine aggregate. Moreover, in case of the RAP was replaced up to 10% of unit cement weight, it was able to be possible to improve fluidity and compressive strength of mortar.

• BMB Reports
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• v.42 no.11
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• pp.764-768
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• 2009

The tandem ubiquitin-interacting motif (UIM) domain located at the N-terminus of Receptor Associated Protein 80 (RAP80) plays a crucial role in ionizing radiation (IR)-induced DNA damage response. RAP80 translocates to sites of IR-induced DNA damage through interaction of its UIM domain with ubiquitinated H2A and Lys63-linked polyubiquitin chains. The exact mechanism, however, through which RAP80 associates with Lys63-linked polyubiquitin chains is not clear. Here, we show by in vitro GST-pull down assays that modifying the linker region between the tandem ubiquitin binding domains of RAP80 changes the binding affinity for Lys63-linked polyubiquitin chains and affects translocation to sites of DNA breaks. Based on these findings, we suggest that the length of the linker region between the tandem ubiquitin binding domains of RAP80 may be a key factor in the binding of RAP80 with Lys63-linked polyubiquitin chains as well as in the translocation of RAP80 to DNA break sites.

• IMMUNE NETWORK
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• v.9 no.2
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• pp.35-40
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• 2009

Although Rap GTPases of the Ras family remained enigmatic for years, extensive studies in this decade have revealed diverse functions of Rap signaling in the control of cell proliferation, differentiation, survival, adhesion, and movement. With the use of gene-engineered mice, we have uncovered essential roles of endogenous Rap signaling in normal lymphocyte development of both T- and B-lineage cells. Deregulation of Rap signaling, on the other hand, results in the development of characteristic leukemia in manners highly dependent on the contexts of cell lineages. These results highlight crucial roles of Rap signaling in the physiology and pathology of lymphocyte development.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.6
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• pp.1101-1107
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• 2016

As an increase in the amount of RAP, there are growing interests in recycling asphalt concrete. In case of using the RAP as recycled asphalt pavement, it tends to be quality deteriorate. Therefore, the amount of RAP using is advised to be limited or has to be used with rejuvenator. In this study, asphalt mixture containing WMRA was analyzed to be used up to 50% for the sake of convenience on the process. As the results of evaluation, there was no significant difference in case of using 30% of RAP in the test of Marshall stability. However, in case of WMRA using up to 50%, it was satisfied criteria by flow value at 34.7. Further, result of toughness test was found that the crack resistance showed 55% higher than using straight asphalt when using WMRA binder up to 50%. According to the results of directly comparing crack resistance through repeated direct tensile test, it was shown that the fatigue crack resistance of WMRA pavement was increased by 263%. Therefore, it was shown that WMRA binder was effective in recycling RAP because WMRA binder could increase the percentage of RAP using up to 50%.