• Animal cells and systems
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• v.3 no.1
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• pp.73-78
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• 1999

Although there are several methods for the preparation of mitochondrial DNA (mtDNA) from mammalian tissues, most are relatively long ultracentrifugation or manipulations by a small-scale method. We escribed a rapid method for large-scale extraction of mtDNA from human placental and horse liver tissues. The method is based on the preparation and homogenization of tissues, urification of crude mitochondria by differential centrifugations and isolation of mtDNA by alkaline Iysis. It was improved from Pre-existing methods by replacing some steps with simpler ones and discarding many others. This method gives a high yield of pure mtDNA(approximately 1-5mg from one placenta; ca. 400-600 g wet weight), depending on its sources (fresh tissue gave better results than frozen one). The resulting mtDNA indicated that this method can yield mtDNA in sufficient purity and quantity to identify the direct restriction analysis on agarose gel, random-primed labeling as a probe, and end labeling. Therefore, the method is ideal for obtaining good mtDNA samples to conduct routine restriction fragment length polymorphism (RFLP) analyses of natural populations for genetic studies.

• Journal of Institute of Control, Robotics and Systems
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• v.4 no.2
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• pp.246-255
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• 1998

This paper presents a novel method for real-time malfunction detection of plasma etching process using EPD signal traces. First, many reference EPD signal traces are collected using monochromator and data acquisition system in normal etching processes. Critical points are defined by applying differentiation and zero-crossing method to the collected reference signal traces. Critical parameters such as intensity, slope, time, peak, overshoot, etc., determined by critical points, and frame attributes transformed signal-to symbol of reference signal traces are saved. Also, UCL(Upper Control Limit) and LCL(Lower Control Limit) are obtained by mean and standard deviation of critical parameters. Then, test EPD signal traces are collected in the actual processes, and frame attributes and critical parameters are obtained using the above mentioned method. Process malfunctions are detected in real-time by applying SPC(Statistical Process Control) method to critical parameters. the Real-time malfunction detection method presented in this paper was applied to actual processes and the results indicated that it was proved to be able to supplement disadvantages of existing quality control check inspecting or testing random-selected devices and detect process malfunctions correctly in real-time.

• Animal cells and systems
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• v.16 no.4
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• pp.313-320
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• 2012

Genetic variation was surveyed at four microsatellite loci and 1416 base pairs (bp) of the mitochondrial DNA (mtDNA) cytochrome c oxidase I gene (COI) to clarify the genetic structure of the small yellow croaker, Larimichthys polyactis, in the Yellow and East China Seas, especially regarding four provisional populations, (one Korean and three Chinese populations). Based on microsatellite DNA variations, the estimated expected heterozygosity ($H_E$) in each population ranged from 0.776 to 0.947. The microsatellite pairwise $F_{ST}$ estimates showed no significant genetic differentiation between the populations. MtDNA variations also indicated no genetic structure in L. polyactis, but very high variability. The absence of genetic differentiation among and within populations of L. polyactis may either result from the random migration of the adult or the passive dispersal of the eggs and larvae.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.7
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• pp.892-896
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• 2004

A genetic examination using 14 structural loci and 7 microsatellite markers was carried out among random samples of Hu sheep (Hu), Tong sheep (Tong) and Yantse River Delta White goat (YRD); The mean heterozygosity (H), mean polymorphism information contents (PIC) and mean effective numbers of alleles (Ne) calculated based on the data from the above two types of genetic markers were compared. The standard genetic distances among the three populations based on two types of gene frequencies were calculated and compared. The results show that the mean heterozygosity (H), mean polymorphism information contents (PIC) and mean effective numbers of alleles (Ne) based on 7 microsatellite markers are greater than those based on the structural loci. The standard genetic distances based on structural loci among the three populations are: 0.0268-0.2487, the standard genetic distances based on microsatellite markers are: 0.2321-1.2313. The study indicates that structural and microsatellite markers reflect the genetic variation of the three populations consistently: Tong>Hu>YRD. The differentiation between related species or interpopulations can be expressed more effectively by microsatellite markers than structural markers. Oar FCB11, MAF33, Oar AE101, Oar FCB128 and OarFCB304 can be used as representative loci for research on genetic differentiation between sheep and goat.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.4B
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• pp.255-269
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• 2006

The differentiated services(DiffServ) architecture provides packet level service differentiation through the simple and predefined Per-Hop Behaviors(PHBs). The Assured Forwarding(AF) PHB proposed as the assured services uses the RED-in/out(RIO) approach to ensusre the expected capacity specified by the service profile. However, the AF PHB fails to give good QoS and fairness to the TCP flows. This is because OUT(out- of-profile) packet droppings at the RIO buffer are unfair and sporadic during only network congestion while the TCP's congestion control algorithm works with a different round trip time(RTT). In this paper, we propose an Adaptive Regulating Drop(ARD) marker, as a novel dropping strategy at the ingressive edge router, to improve TCP fairness in assured services without a decrease in the link utilization. To drop packets pertinently, the ARD marker adaptively changes a Temporary Permitted Rate(TPR) for aggregate TCP flows. To reduce the excessive use of greedy TCP flows by notifying droppings of their IN packets constantly to them without a decrease in the link utilization, according to the TPR, the ARD marker performs random early fair remarking and dropping of their excessive IN packets at the aggregate flow level. Thus, the throughput of a TCP flow no more depends on only the sporadic and unfair OUT packet droppings at the RIO buffer in the core router. Then, the ARD marker regulates the packet transmission rate of each TCP flow to the contract rate by increasing TCP fairness, without a decrease in the link utilization.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.4
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• pp.726-747
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• 2013

In order to support a large number of mobile stations (MSs) with statistical multiplexing in cellular networks, a random access scheme is widely used for uplink (UL) bandwidth request (BR). In the design of a random access based BR scheme, there are two important requirements: short connection delay and diverse Quality of Services (QoSs) support. Such requirements are crucial for IMT-Advanced systems like IEEE 802.16m to provide various types of fourth generation (4G) data services. IEEE 802.16m provides advanced UL BR schemes for non-real time polling service (nrtPS) and best-effort (BE) service to meet the requirements of short connection time and multiple QoS level support. In order to provide short connection time and multiple QoS support, three-step and differentiated BR procedures are adopted. In this paper, a novel modelling of IEEE 802.16m contention based BR scheme is proposed that uses a 2-dimensional discrete time Markov chain. Both the short access delay three-step BR procedures and normal five-step BR procedure are considered in the model. Our proposed model also incorporates the IEEE 802.16m differentiated BR procedure. With the proposed model, we extensively evaluate the performance of IEEE 802.16m BR for two different service classes by changing QoS parameters, such as backoff window size and BR timer. Computer simulations are performed to corroborate the accuracy of the proposed model for various operation scenarios. With the proposed model, accurate QoS parameter values can be derived for the IEEE 802.16m contention-based BR scheme.

• The Korean Journal of Food And Nutrition
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• v.17 no.3
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• pp.254-259
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• 2004

Rapid detection of foodbome pathogens is becoming increasingly important. The requirement for faster, more reliable tests has lead to the development of a wide range of rapid methods. Among these methods, the use of systems based on nucleic acid based detection has been increasing since they offer advantages of reduction in test time and more reliable detection or identification. Random Amplification Polymorphic DNA(RAPD) method has been used to fingerprint foodbome microorganisms; Listeria monocytogenes. In this study, 10-mer primer OPG-13(5'-CTCTCCGCCA-3') was used to generate RAPD-PCR for detection of pathogenic L. monocytogenes of Listeria spp. Among 20 primers tested, OPG-13 showed on acceptable result for the differentiation of a pathogenic Listeria from non-pathogenic microorganisms. Pathogenic Listeria, L. monocytogenes(ATCC 15313, 19111, 19112, 19113) showed two bands for 700 bp and 1,500 bp while non-pathogenic bacteria, L. ivanovii, L. grayi, L. murrayi, L. innocua, L. welshimeri, and L. seeligeri had only one band sizing from 2,000 to 2,300 bp. This RAPD method proved to be a valuable to gain important information on sources of pathogenic bacteria in food industry.

• International Journal of Human Ecology
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• v.6 no.2
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• pp.73-83
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• 2005

The purpose of this study was to analyze the needs of future users for services at senior congregate housing and to look into the development directions of the service standards for senior congregate housing. A survey was conducted among middle-aged people in their fifties, who lived in Seoul, using the systematic random sampling method. The final sample included 498 respondents. The survey was conducted from November 3, 2003 to November 14, 2003. The results of this study were as follows. Firstly, as the need for housing services were high, it was quite clear that various services at senior congregate housing should be provided. Secondly, a service differentiation strategy should be proposed to meet the characteristics of the users. Thirdly, it was hereby suggested that service standards should be established in the following manner considering the relationship between the needs for services and the income level directly influencing the provision of services: 'Basic services' to be provided regardless of income, 'Optional services' to be selectable among the preferable services per income level, and 'Supportive services' to be provided for low-income people.

• Journal of Plant Biology
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• v.39 no.3
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• pp.223-229
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• 1996

The genetic variation in Korean evening primorse (Oeothera odorata L.) populations was examiend to estimate the level of allozyme variation within populatons using starch gel electrophoresis. 7 of 13 loci (Adh, Est-1, Est-2, Mdh-2, Pgd-2, Pgm-1, and Idh) revealed (Ps=43.2%) were polymorphic. The mean number of alleles per locus (A) and polymorphic locus (Ap) for populations were 1.64 and 2.46, respectively. The effective number of alleles (Aep) within populations relatively was low ranging from 1.08 to 1.22 with a mean of 1.14. Within populations, the mean number of allele per polymorphic loci (Ap) was 2.46, the mean number of alleles per locus (A) was 1.64, and the mean genetic diversity was 0.093. About 2.7% of the total allozyme diversity resided among populations (Mean GST=0.0274). FIS, a measure of the deviation from random mating within 13 populations, was relative low (mean FIS=0.03636). The indirect estimate of gene flow, based on the mean GST, was high (Nm=8.88). Estimates of gene flow were consistent with low levels of genetic differentiation among populations.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.682-688
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• 1998

Fungicide resistance of 48 isolates of Botrytis cinerea collected from citrus in Cheju was investigated and genetic diversity was analyzed with random amplified polymorphic DNA(RAPD). High levels of resistance to benzimidazole fungicides benomyl and thiophanate-methyl and N-phenylcarbamate fungicide diethofencarb were observed. Negative cross resistance was clear between benzimidazole and N-phenylcarbamate fungicides, and multiple resistance to the fungicides was also observed. There was cross resistance among the dicarboximide fungicides procymidione, vinclozolin and iprodione as it was observed between the benzimidazole fungicides benomyl and thiophanate-methyl. The lowest levels of resistance were to the dicarboximide fungicides, but no sensitive isolate to polyoxin B was observed. The isolates showed genetically diverse RAPD profiles according to the geographic origin collected, but there was no significant correaltion between RAPD profiles of genomic DNA and the levels of fungicide resistance of the isolates. The isolates showed genetically diverse RAPD profiles, indicating that genetic differentiation had already occurred in the populations of B. cinerea distributed in Cheju.