• Title/Summary/Keyword: random differentiation

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Genetic differentiation and antioxidant activities of Bouea macrophylla Griffith in Nakhon Nayok province

  • Thummajitsakul, Sirikul;Silprasit, Kun
    • Journal of Applied Biological Chemistry
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    • v.60 no.1
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    • pp.41-47
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    • 2017
  • Genetic differentiation and antioxidant activities in ethanolic extracts from leaves of Bouea macrophylla Griffith were determined. The result revealed genetic differentiation among sour ma-praang, ma-yong and sweet ma-praang of B. macrophylla Griffith (${\phi}_{PT}=0.772$, p-value=0.000). In addition, high genetic diversities were found in sour ma-praang and sweet ma-praang populations (P: 51.4 and 57.1 %; He: 0.1900.035 and 0.2240.036, respectively), but low genetic diversity was found in ma-yong population (P: 8.6 %; He: 0.0350.021). Total phenolic contents of sour ma-praang, ma-yong and sweet ma-praang were estimated as $680.51{\pm}89.81$, $701.03{\pm}59.89$ and $530.85{\pm}41.23mg$ gallic acid/g extract, respectively. Free radical scavenging activities of sour mapraang, ma-yong and sweet ma-praang were found (1/EC50=4.17, 1.43 and 1.37, respectively) corresponding with metalchelating activities (1/EC50=0.83, 0.65 and 0.17, respectively). Therefore, the obtained data may be applied to cultivation and utilization of their leaves as source of natural phenolics and antioxidants.

Priority-based Intelligent Uplink Random Access Scheme for Fourth-generation Cellular Systems and Its Standardization (4세대 이동통신시스템을 위한 우선순위 기반 지능적 상향링크 랜덤 접속 방법 및 표준화)

  • Moon, Jung-Min;Lee, Ho-Won;Cho, Dong-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.12B
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    • pp.1623-1635
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    • 2011
  • Fourth-generation (4G) cellular communication systems must provide different quality-of-service (QoS) to users according to the service type, membership class, and usage case of each user. For the purpose of user's QoS differentiation, we propose an enhanced uplink random access scheme for bandwidth request. More specifically, we divide a bandwidth request channel into two parts: One is a primary region and the other is a secondary region. Then, each region has a unique minimum access class that allows only specific users to perform random access through that region. By doing so, we can reduce collision probability and increase the success probability of bandwidth request. From the perspective of standardization, we presented this scheme in the IEEE 802.16m Session #66 held on March 2010. As a result, the concept of the proposed scheme and required messages were defined in the 802.16m standard.

Achieving Relative Loss Differentiation using D-VQSDDP with Differential Drop Probability (차별적이니 드랍-확률을 갖는 동적-VQSDDP를 이용한 상대적 손실차별화의 달성)

  • Kyung-Rae Cho;Ja-Whan Koo;Jin-Wook Chung
    • Proceedings of the Korea Information Processing Society Conference
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    • 2008.11a
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    • pp.1332-1335
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    • 2008
  • In order to various service types of real time and non-real time traffic with varying requirements are transmitted over the IEEE 802.16 standard is expected to provide quality of service(QoS) researchers have explored to provide a queue management scheme with differentiated loss guarantees for the future Internet. The sides of a packet drop rate, an each class to differential drop probability on achieving a low delay and high traffic intensity. Improved a queue management scheme to be enhanced to offer a drop probability is desired necessarily. This paper considers multiple random early detection with differential drop probability which is a slightly modified version of the Multiple-RED(Random Early Detection) model, to get the performance of the best suited, we analyzes its main control parameters (maxth, minth, maxp) for achieving the proportional loss differentiation (PLD) model, and gives their setting guidance from the analytic approach. we propose Dynamic-multiple queue management scheme based on differential drop probability, called Dynamic-VQSDDP(Variable Queue State Differential Drop Probability)T, is proposed to overcome M-RED's shortcoming as well as supports static maxp parameter setting values for relative and each class proportional loss differentiation. M-RED is static according to the situation of the network traffic, Network environment is very dynamic situation. Therefore maxp parameter values needs to modify too to the constantly and dynamic. The verification of the guidance is shown with figuring out loss probability using a proposed algorithm under dynamic offered load and is also selection problem of optimal values of parameters for high traffic intensity and show that Dynamic-VQSDDP has the better performance in terms of packet drop rate. We also demonstrated using an ns-2 network simulation.

Primers for typing Listeria spp. using Random Amplified Polymorphic DNA (RAPD) ANalysis (Listeria spp.의 RAPD typing을 위한 Primer의 분리력 비교)

  • 임형근;홍종해;박경진;최원상
    • Journal of Food Hygiene and Safety
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    • v.18 no.2
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    • pp.67-72
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    • 2003
  • Random amplified Polymorphic DNA (RAPD) analysis Is based on the amplification of random DNA segment using a single arbitrary primer. Polymorphic DNA patterns identified by this method can be used for typing Listeria monocytogenes. To select the primers for RAPD typing Listeria spp., the performance of 31 primers were compared by analyzing 13 Listeria spp. reference strains. Reproducible electrophoresis patterns were obtained. Among 31 primers, 6 primers (primer 6, HLWL74, UBC155, UBC127, Lis5, Lis11) showed better differentiation, when discrimination index, band clarity, band number, difficulty of band scoring were considered than the others. These primers will be useful far typing Listeria spp. in the future. Currently, we are under investigation for the RAPD typing of contaminated L. monocytogenes for the risk analysis of pork processing plant using these primers.

Genetic Differentiation among Sheep Populations from Near-sea Mainland in East Asia

  • Lu, S.X.;Chang, H.;Du, L.;Tsunoda, K.;Ji, D.J.;Sun, W.;Yang, Z.P.;Chang, G.B.;Mao, Y.J.;Wang, Q.H.;Xu, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.10
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    • pp.1360-1365
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    • 2004
  • Using the method of 'random sampling in typical colonies of the central area of the habitat', 60 Small-tailed Han sheep were obtained in Jining city, Shangdong province. The variations of Small-tailed Han sheep at 12 structural loci encoding blood proteins were detected by several electrophoresis techniques and their gene frequencies were then estimated. The same data of four other sheep populations from Near-sea Mainland in East Asia were cited for the analysis of genetic differentiation. The average heterozygosities of five populations, namely Kharkhorin sheep, Ulaanbaatar sheep, Small-tailed Han sheep, Hu sheep and Cham Tribe sheep were 0.3447, 0.3285, 0.3157, 0.3884 and 0.2300, respectively. The coefficient of gene differentiation among four populations, Kharkhorin sheep, Ulaanbaatar sheep, Small-tailed Han sheep and Hu sheep, was 0.045557, and that between these four breeds and Cham Tribe sheep was 0.088005, indicating that the level of gene differentiation among the former four sheep populations of Mongolian group was comparatively lower than that between Cham Tribe sheep and other four sheep populations. The origin of Cham Tribe sheep deserve further research. The documentary research on the evolution of Small-tailed Han sheep and Hu sheep from Mongolian sheep was further verified by the biochemical experiments in the study. It was reasonably deduced that Hu sheep, Small Tailed Han sheep and Cham Tribe sheep were decreasingly influenced by the bloodline of Mongolian sheep.

Evaluation of Genetic Differentiation of Albizia lucida Populations from Eastern Region of the Indian Sub-continent by ISSR Markers

  • Aparajita, Subhashree;Rout, G.R.
    • Journal of Forest and Environmental Science
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    • v.24 no.1
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    • pp.27-34
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    • 2008
  • Level and distribution of genetic diversity in seven populations of Albizia lucida Benth. in eastern region of the Indian sub-continent were estimated using ISSR markers. Relatively higher level of genetic diversity within populations was observed in seven populations of A. lucida (mean of 0.38). From the result of AMOVA, majority of genetic diversity was allocated within populations (96.2%) resulting in a moderate degree of population differentiation. The observed distribution pattern of I-SSR variant among the populations was coincided with the typical pattern of long-lived woody tree species. Genetic relationships among the populations, reconstructed by UPGMA method, revealed two genetic groups. The population of Anugul and Bargarh turned out to be the most closely related despite a distance location between them. These formations will be of great value in the development of conservation plans for species exhibiting high levels of genetic differentiation due to fragmentation, such as indication of conservation unit size, which populations should be chosen as priority in conservation plans and which samples should be introduced in areas with a low number of individuals of A. lucida.

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Structural Differentiation of the Connective Stalk in Spirodela polyrhiza (L.) Schleiden

  • Kim, InSun
    • Applied Microscopy
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    • v.46 no.2
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    • pp.83-88
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    • 2016
  • Structural differentiation of the connective stalk in giant duckweed, Spirodela polyrhiza, was examined to reveal the anatomical and ultrastructural characteristics within reduced shoot. The study focuses primarily on structural features of the connective stalk (CT), which connect offspring to their mother fronds. Photoautotrophic offspring fronds remained connected by stalks to mother fronds in the reproductive pockets until separation. The CT originated from the meristematic region of the abaxial frond and joined the fronds laterally with two abscission layers. The most notable features of the CT were polymorphic mitochondria, random occurrences of fibrillar structures in intercellular spaces, and great variability in cell wall thickness. Vascular tissues in CTs were highly reduced, demonstrating only a central vascular strand. Grana with 2 to 4 thylakoids and starch grains were found in the chloroplasts. A chlorophyll assay indicated high chlorophyll concentrations in daughter fronds and low concentrations in CTs. The frond and CT, while physically connected to each other, functioned independently. Despite great reduction in S. polyrhiza, the CT has proven to be very efficient for separating offspring from the mother frond, which lends to its capacity for rapid vegetative reproduction. The ultrastructural aspects of CTs in S. polyrhiza were characterized for the first time in this study.

Molecular Characterization of a Protein Kinase Gene in Chiness Cabbage(Brassica campestrics subsp. napus var. pekinensis)

  • Jeong, Sang-Ho;Ahn, Ji-Hoon;Lee, June-Seung;Lee, Jong-Seob
    • Animal cells and systems
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    • v.1 no.1
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    • pp.135-142
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    • 1997
  • Random sequencing of expressed sequence tags in roots of Chinese cabbage led to isolation of a partial cDNA clone, BR77, which encoded a putative protein kinase. Using the BR77 cDNA as a probe, we isolated a full-length cDNA encoding the Brassica campestris protein kinase 1 (Bcpk1). The Bcpt1 cDNA contained one open reading frame encoding a polypeptide of 439 amino acids. The putative polypeptide consisted of a short N-terminal region and a protein kinase catalytic domain. The catalytic domain of Bcpkl showed a high homology to cAMP- and calcium- phospholipid-dependent subfamilies of serine/threonine protein kineses. Eleven major catalytic domains in protein kineses were well conserved in Bcpk1. However, Bcpk1 contained a unique nonhomologous intervening sequence between subdomains VII and VIII, which was not found in protein kineses of animals and lower eukaryotes. Genomic DNA gel blot analysis showed that Bcpt1 genes might be present as three copies in the Chinese cabbage genome. These imply that Bcpk1 belongs to a plant-specific serine/threonine protein kinase subfamily.

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A Study on the Estimation Method of the Wheel Acceleration (차륜 가속도 예측방법에 대한 연구)

  • 김중배;민중기
    • Transactions of the Korean Society of Automotive Engineers
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    • v.5 no.2
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    • pp.120-126
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    • 1997
  • In this study, an effective estimation method of wheel acceleration is presented. The wheel acceleration is mainly used in the ABS(anti-lick brake system) and the TCS(traction control system). The wheel acceleration is a derivative term of the wheel speed which is generally measured by the wheel speed sensors. The results of a simple differentiation of the signal and an observation of the signal by Kalman filter show that Kalman filter has better performance than the simple differentiation. The differentiated sine signal which is contaminated with random noise shows a rugged signal compared with the signal which is filtered by the Kalman filter. The covariance of the differentiated signal is higher than that of the Kalman-filtered signal, too. The presented Kalman filter technique shows an effective way of solution to get the estimated wheel acceleration value which is sufficient to be applied to ABS or TCS control algorithms.

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Cultural Characteristics and Genetic Diversity of Rhizina undulata Isolates by Random Amplified Polymorphic DNA(RAPD) (리지나뿌리썩음병균 분리주들의 배양 특성 및 RAPD에 의한 유전적 다양성 분석)

  • Lee, Sang Yong;Lee, Sun Keun;Lee, Jong Kyu;Kim, Kyung Hee;Lee, Seung Kyu
    • Journal of Korean Society of Forest Science
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    • v.95 no.4
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    • pp.388-392
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    • 2006
  • To investigate physiological characteristics and genetic diversity of Rhizina undulata isolates distributed in Korea, cultural characteristics and random amplified polymorphic DNA (RAPD) of 13 Rhizina undulata isolates from Pinus densiflora and P. thunbergi stands were analyzed. There were no correlations between the host species of R. undulata isolates and the mycelial growth of R. undulata isolates on culture media supplemented with water-soluble extract from the two different host species, i.e., Pinus densiflora and P. thunbergi. Genetic diversity of genomic DNA from 13 R. undulata isolates was analyzed by RAPD using 12 random primers. There was no differentiation in RAPD profiles among the isolates from Korea. But, there was some differentiation in RAPD profiles between Korean isolates and Japanese isolates, with 88% homology by phylogenetic tree analysis.