Browse > Article

Primers for typing Listeria spp. using Random Amplified Polymorphic DNA (RAPD) ANalysis  

임형근 (동국대학교 생명공학과)
홍종해 (강원대학교 수의학과)
박경진 (한국보건산업진흥원 HACCP팀)
최원상 (동국대학교 생명공학과)
Publication Information
Journal of Food Hygiene and Safety / v.18, no.2, 2003 , pp. 67-72 More about this Journal
Abstract
Random amplified Polymorphic DNA (RAPD) analysis Is based on the amplification of random DNA segment using a single arbitrary primer. Polymorphic DNA patterns identified by this method can be used for typing Listeria monocytogenes. To select the primers for RAPD typing Listeria spp., the performance of 31 primers were compared by analyzing 13 Listeria spp. reference strains. Reproducible electrophoresis patterns were obtained. Among 31 primers, 6 primers (primer 6, HLWL74, UBC155, UBC127, Lis5, Lis11) showed better differentiation, when discrimination index, band clarity, band number, difficulty of band scoring were considered than the others. These primers will be useful far typing Listeria spp. in the future. Currently, we are under investigation for the RAPD typing of contaminated L. monocytogenes for the risk analysis of pork processing plant using these primers.
Keywords
Random amplified polymorphic DNA (RAPD); Listeria spp.; Listeria monocytogenes;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Seeliger, H.P.R. and Hohne, K.: Serotyping of Listeria monocytogenes. In Method in Microbiology (Bergan, T and Norris, J.R. Eds), Vol. 13, Academic Press, New York, p. 31-49 (1979)
2 Franciosa, G., Pourshaban, M., Gianfranceschi, M., and Aureli, P.: Genetic typing of human and food isolates of Listeria monocytogenes from episodes of listeriosis. Eur. J. Epidemiol. 14, 205-210 (1998)   DOI   ScienceOn
3 Choi, W.S. and Hong, C.-H.:Rapid enumeration of Listeria monocytogenes in milk using competitive PCR. International J. Food Microbiol. 84, 79-85 (2003)   DOI   ScienceOn
4 Chansiripomchai, N., Ramasoota, P., Bangtrakulnonth, A., Sasipreeyajan, J, Svenson, S.B.: Application of randomly amplified polymorphic DNA (RAPD) analysis for typing avian Salmonella entrica subsp. enterica. FEMS Immunology and Medical Microbiol. 29, 221-225 (2000)   DOI   ScienceOn
5 Milleman, Y., Lesage-Descauses, M.-C., Lafont, J.-P. and Chaslus-Dancla, E.: Comparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella. FEMS Immunol. Medical Microbiol. 14, 129-134 (1996)   DOI
6 Miyata, M., Aoki, T., Inglis, V., Yoshida, T. and Endo, M.: RAPD analysis of Aeromonas salmonicida and Aeromonas hydrophila. : J. Appl. Bacteriol. 79, 181-185 (1995)   DOI
7 Lehner, A, Loncarevic, S., Wagner, M., Kreike, J. and Brandl, E.: A rapid differentiation of Listeria monocytogenes by use of PCR-SSCP in the listeriolysin O (hlyA) locus. J Microbiol. Methods 34, 165-171 (1999)   DOI   ScienceOn
8 Hunter, P.R. and Gaston, M.A.: Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26, 2465-2466 (1998)
9 Mare, L., Dicks, L.M.T. and van der Walt, M.L.: Characterization of South African isolates of Salmonella enteritidis by phage typing, numerical analysis of RAPD-PCR banding patterns and plasmid profiles. International J. Food Microbiol. 64, 237-245 (2001)   DOI   ScienceOn
10 Brosch, R., Brett, M., Catimel, B., Luchansky, J.B., Ojeniyi, B. and Rocourt, J.: Genomic fingerprinting of 80 strains from the WHO multicenter international typing study of Listeria monocytogenes via pulsed-field gel electrophoresis (PFGE). International J. Food Microbiol. 32, 343-355 (1996)   DOI   ScienceOn
11 Gravesen, A., Jacobsen, T., Moller, P.L., Hansen, F., Larsen, AG. and Knochel, S.:Genotyping of Listeria monocytogenes: comparision ofRAPD, ITS, and PFGE. International J. Food Microbiol. 57, 43-51 (2000)   DOI   ScienceOn
12 Martinez, I., Rorvik, L.-M., Brox, V., Lassen, J., Seppola, M., Gram, L. and Fonnesbech-Vogel, B.: Genetic variability among isolates ofListeria monocytogenes from food products, clinical samples and processing environments, estimated by RAPD typing. International J. Food Microbiol. in press (2003)
13 Destro, M., Leitao, M.F.F. and Farber, J.M.: Use of molecular typing methods to trace the dissemination of Listeria monocytogenes in a shrimp processing plant. Appl. Environ. Microbiol. 62(2), 705-711 (1996)
14 Byun, S.-K., Jung, S.C. and Yoo, H.S.:Random amplification of polymorphic DNA typing of Listeria monocytogenes isolated from meat. International J. Food Microbiol. 69, 227-235 (2001)   DOI   ScienceOn
15 Van de Peer, Y., and De Wachter, R.: TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. Applic. Biosci. 10, 569-570 (1994)
16 WilIams, J.G.K., Kubelin, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V.: DNA polymorphism amplified by artitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535 (1990)   DOI   ScienceOn
17 Laconcha, I., Lopez-Molina, N., Rementeria, A., Audicana, A, Perales, I. and Garaizar, J.: Phage typing combined with pulsed-field gel electrophoresis and random amplified polymorphic DNA Increases discrimination In the epidemiological analysis of Salmonella enteritidis strains. International J. Food Microbiol. 40, 27-34 (1998)   DOI   ScienceOn
18 Seeliger, H.P.R. and Weishimer, H.J. In Bogy's Manual of Determining Bacteriology, 8th edn. The Williams & Wilkins Company, Baltimore, p. 593 (1974)
19 Wang, C. and Hong, C.: A rapid PCR-based hybridization assay for the detection of Listeria monocytogenes in channel catfish. Food Microbiol. 16, 291-297 (1999)   DOI   ScienceOn
20 Manzano, M., Cocolin, L., Cantoni, C. and Comi, G.: A rapid method for the identification and partial serotyping of Listeria monocytogenes in food by PCR and restriction enzyme analysis. International J. Food Microbiol. 42, 207-212 (1998)   DOI   ScienceOn
21 Giovannacci, I., Ragimbeau, C, Queguiner, S., Salvat, G., Vendeuvre, J.-L., Carlier, V. and Ermel, G.: Listeria monocytogenes in pork slaughtering and cutting plants : use of RAPD, PFGE and PCR-REA for tracing and molecular epidemiology. International J Food Micribiol. 53, 127-140 (1999)   DOI   ScienceOn
22 Pourshaban M., Gianfranceschi, M., Gattuso, A, Menconi, F. and Aureli, P.: Identification of Listeria monocytogenes contamination sources in two fresh sauce production plants by pulse-field gel electrophoresis. Food Microbiol. 17, 393-400 (2000)   DOI   ScienceOn
23 Tsen, H., Hu, H.H., Lin, J.S., Huang, C.H. and Wang, T.K.: Analysis of the Salmonella typhimurium isolates from foodpoisoning cases by molecular subtyping methods. Food Microbiol. 17, 143-152 (2000)   DOI   ScienceOn
24 Kerouanton, A., Brisabois, A., Denoyer, E., Dilasser, F., Grout, J., Salvat, G. and Picard, B.:Comparison of five typing methods for the epidemiological study of Listeria monocytogenes. International J. Food Microbiol. 43, 61-71 (1998)   DOI   ScienceOn
25 Lin, A.W., Usera, M.A., Barret, T.J. and Goldsby, R.A: Application of randomly amplified polymorphic DNA analysis ti differentiate strains of Salmonella enteritidis. J. Clin. microbiol. 34, 870-876 (1996)
26 Jaradat, Z.W., Schutze, G.E. and Bhunia, A.K.: Genetic homogenety among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes. International J. Food Microbiol. 76, 1-10 (2002)   DOI   ScienceOn
27 Hitchins, A.D. Listeria monocytogenes. In Bacteriological Analytical Manual. 7th edn. AOAC International, Arlington, VA, USA, p.141-151 (1992)
28 Link, W., Dixens, C, Singh, M., Schwall, M. and Melchinger, A.E.: Genetic diversity in European and Mediterranean faba bean germ plasm revealed by RAPD markers. Theor. Appl. Genet. 90, 27-32 (1995)
29 이우주: 의학사전, 아카데미서적, 서울, pp, 612 (1992)