• Fisheries and Aquatic Sciences
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• 제15권3호
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• pp.183-190
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• 2012

The purpose of this study was the purification and characterization of an angiotensin I converting enzyme (ACE) inhibitory peptide purified from enzymatic hydrolysates of rainbow trout Oncorhynchus mykiss muscle. After removal of lipid, the approximate composition analysis of the rainbow trout revealed 24.4%, 1.7%, and 68.3% for protein, lipid, and moisture, respectively. Among six hydrolysates, the peptic hydrolysate exhibited the highest ACE inhibitory activity. We attempted to purify ACE inhibitory peptides from peptic hydrolysate using high performance liquid chromatography on an ODS column. The $IC_{50}$ value of purified ACE inhibitory peptide was $63.9{\mu}M$. The amino acid sequence of the peptide was identified as Lys-Val-Asn-Gly-Pro-Ala-Met-Ser-Pro-Asn-Ala-Asn, with a molecular weight of 1,220 Da, and the Lineweaver-Burk plots suggested that they act as a competitive inhibitor against ACE. Our study suggested that novel ACE inhibitory peptides purified from rainbow trout muscle protein may be beneficial as anti-hypertension compounds in functional foods.

• 한국어류학회지
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• 제28권3호
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• pp.141-146
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• 2016

본 연구에서는 넙치 유래 VHSV (genotype IVa)에 의한 무지개송어의 영향을 조사하기 위하여 다양한 조건에서 감염 실험을 실시하였다. 3개의 다른 로트의 담수 무지개송어 치어에 VHSV ($10^{6.3-7.3}TCID_{50}$/fish)를 복강 주사한 결과, 15% 이하의 누적 폐사율이 관찰되었다. 무지개송어 (in vivo)를 사용하여 VHSV를 계대 배양한 후 감염 실험을 실시한 결과, VHSV를 5회 계대 배양하여도 독력의 변화는 관찰되지 않았다. 해수에 사육 중인 송어 (성어)에 넙치 유래 VHSV ($10^{5.3}TCID_{50}$/fish와 $10^{6.3}TCID_{50}$/fish)를 복강 주사한 결과, 폐사어는 관찰되지 않았고, 어체 내에서도 VHSV는 검출되지 않았다. 이상의 결과, 해수 송어 (성어)에서는 넙치 유래 VHSV의 병원성이 확인되지 않았으나 담수 무지개송어 치어에서는 낮은 독력을 나타내었다.

• 생명과학회지
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• 제5권2호
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• pp.14-14
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• 1995

The effect of lipoxygenase (LOX) on the oxidation and co-oxidation of lipid fraction was studied in the model system of rainbow trout. For the reaction in model system 1 g of lipid fraction and 50mL of enzyme extract(LOX, 140 unit in 50mL phosphate buffer solution at pH 7,4)), which were obtained from rainbow trout, were homoginized in the presence of Tween 20 and kept at 23$\circ$C for 3 days. The activity of LOX was decreased to 43% of initial level during the reaction in the model system. The initial composition of rainbow trout lipid was showed to be consisted of trigliceride(TG;82%) and free fatty acid(FFA;0.1%), while this converted to 59% of TG and 20% of FIFA, respectively after reaction in model system. Change of fatty acid composition was also observed and the content of linoleic acid, one of the major fatte acids, was decreased to 13% from 54% in the content of total fatty acids after reaction. The carotenoids in rainbow trout were composed of 0.4% $\alpha$-carotene, 1.6% $\beta$ -carotene, 80% canthaxanthin, 7% lutein and 11% zeaxanthin, thus the canthaxanthin was the major component. This canthaxanthin was the most degraded carotenoid by lipoxygenase catalyzed co-oxidation during the reaction. On the other hand the tocopherol isomers found in the rainbow trout were $\alpha$ and $\beta$ -tocopherol, and $\alpha$-tocopherol had a higher degradation rate by the lipoxygenase catalyzed co-oxidation than of $\beta$-tocopherol in the reaction of model system.

• 한국어류학회지
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• 제27권3호
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• pp.183-188
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• 2015

본 연구에서는 무지개송어의 해수면 양식장으로의 이동에 따라 나타날 수 있는 어류 병원성 바이러스의 영향을 알아보기 위해, 담수 무지개송어 유래 병원체인 infectious hematopoietic necrosis virus (IHNV)와 infectious pancreatic necrosis virus (IPNV)를 사용하여 주요 해산 양식 어종인 넙치, 조피볼락, 돌돔, 참돔 및 능성어에 주사한 후 병원성 및 감염 여부를 조사하였으며, 또한 해산 양식 어종 유래 병원체인 marine birnavirus (MABV), hirame rhabdovirus (HIRRV) 및 nervous necrosis virus (NNV)를 사용하여 무지개송어에 대한 병원성 및 감염 여부를 조사하였다. IHNV와 IPNV는 주요 해산 양식 어종에 감염되는 것으로 확인되었으며, 또한 무지개송어는 해산 유래 위해 병원체인 MABV와 NNV에 감염되거나 HIRRV에 의해 폐사되는 것으로 확인되었다. 이상의 연구 결과, 해수사육 무지개송어는 어류 병원성 바이러스들에 의해 영향 받을 수 있을 것으로 사료되었다.

• 영재교육연구
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• 제21권1호
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• pp.39-56
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• 2011

본 연구는 무지개를 활용한 과학영재활동에서 과학영재들이 나타내는 특징을 분석하는 데 목적을 두었다. 이를 위해 지방 소재 대학교 부설 과학영재교육원 소속 중학생 23명을 연구대상으로 설정하였다. 분석 결과, 첫째, 과학영재의 다양한 광학 관련 개념과 사고 유형이 나타났다. 과학영재들은 무지개 모양 그대로 빛이 그 곳에 존재할 것이라고 생각하기도 하며, 물방울에서 빛의 분산이 프리즘에서처럼 단순한 과정일 것이라고도 생각하였다. 둘째, 과학영재들은 이 활동을 통해 다양한 이론을 구성하고 다양한 과학적 상상 유형을 표현하였다. 과학영재들은 자신의 이해를 바탕으로 무지개의 모양과 색에 대한 다양한 이론을 구성해 제시했다. 새로운 무지개를 제시하는 과제에서도 기존 무지개의 모양이나 색 등이 가지는 원래 현상의 특징을 변화시키거나 생성 조건을 변화시키는 두 가지 전략을 사용해 다양한 무지개를 제안하였다. 덧붙여 무지개를 활용한 통합적 과학활동과 후속과제에 대하여 논의하였다.

• 정보보호학회논문지
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• 제29권4호
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• pp.697-708
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• 2019

본 논문에 이차식 기반 서명인 Rainbow를 8비트 MCU(Microcontroller Unit)에 적용하기 위해 최적화 하는 방안을 검토하고 구현한다. 양자 컴퓨터가 개발되면서 기존의 암호 알고리즘 특히, 서명 기법의 보안성을 위협함에 따라 IoT 기기에도 양자내성을 갖춘 서명 기법을 적용해야할 필요성이 있다. 현재 제안된 양자내성암호는 격자 기반, 해쉬 기반, 코드 기반 그리고 다변수 이차식 기반 암호 알고리즘 및 서명 기법들이 있는데, 특히 다변수 이차식 서명기법은 기존의 서명 기법과 비교해 속도가 빨라 IoT 기기에 적합하다. 그러나 키의 길이가 크고 연산이 많아 IoT 기기 중 메모리와 성능에 큰 제약이 있는 8비트 MCU에는 기존의 구조 그대로 구현하기 어려워 이에 적합한 최적화가 필요하다. 따라서 본 논문에서는 다변수 이차식 서명 기법인 Rainbow를 8비트 MCU에 키를 나누어 저장하는 방안과 연산방식을 최적화하여 메모리 소모가 적고 연산 속도가 빠르게 알고리즘을 개선하고, 구현해본 후 각 최적화 방식에 따른 성능을 비교한다.

• 혜화의학회지
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• 제15권1호
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• pp.87-95
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• 2006

Objective : We suggested the clinical effect of Rainbow therapy on Bell's palsy. Methods : 20 Bell's palsy patients were divided into two groups. One group(A group) was treated by acupunture and the other group(B group) was treated by acupunture and Rainbow therapy. The effect of these treatments was evaluated by Yanagihara's unweighted grading system and House-Brackmann grading system. Results and Conclusions : In Yanagihara's unweighted grading system After 1 week and 2weeks treatment, group B marked more higher than group A in treatment outcome. We discovered that it is significant differences between two groups. After 3 weeks treatment, group B marked more higher than group A in treatment outcome but it is not significant differences between two groups. In House-Brackmann's facial nerve grading system, After 1 week treatment, group B marked more higher than group A in treatment outcome. We discovered that it is significant differences between two groups. After 2 weeks and 3 weeks treatment, group A marked more higher than group A in treatment outcome but it is not significant differences between two groups.

• 생명과학회지
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• 제17권9호통권89호
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• pp.1244-1247
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• 2007

Effects of muscle fluid concentrates from Styela clava on fish diets were investigated in rainbow trout(Oncorhynchus mykiss). Fish were fed on one of the isonitrogenous(48%) and isolipic(20%) feed containing 5 to 20% of muscle fluid concentrates for 8 weeks. Hematological parameters such as hemoglobin, hematocrit, albumin, glucose, total bililubin, triglyceride and glutamic pyruvate transaminase of the rainbow trout which were fed on the diets varied, but no specific trend became apparent. However, glutamic oxaloacetic transaminase value was significantly higher than that of normal fishes. Therefore, concentrated ascidian fluid which is normally discarded, can be combined with commercial diets for the normal growth of rainbow trout.

• 한국수산과학회지
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• 제50권5호
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• pp.624-629
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• 2017

A rainbow trout Onchorhynchus mykiss farm located in Gangwon province, South Korea, experienced approximately 10% mortality in June 2017. Most diseased fish had a markedly distended, gas-filled stomach, and exhibited abnormal behavior at the water surface. In this study, we attempted to identify the cause of stomach distension syndrome in those rainbow trout. The stomach of most of the affected fish were full of unidentified gases and some exudate, and yeast was isolated from the stomach mucosa. Pure cultures of yeast were identified using a multilocus sequence typing scheme based on 18S rRNA, internal transcribed spacers, large subunit rRNA, and the gene encoding the largest subunit of RNA polymerase II (RPB1). The RPB1 gene sequences were compared with those of related species available in a database. The yeast was identified as Scheffersomyces coipomoensis (Candida coipomoensis) based on sequence analyses. This is the first study to reveal that Sch. coipomoensis is a potential causative agent of stomach distension syndrome in farmed rainbow trout. Our results will be helpful for future related studies, and indicate that farmers and stakeholders should observe this emerging disease closely.

• Fisheries and Aquatic Sciences
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• 제1권1호
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• pp.19-23
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• 1998

Effects of pituitary and thyroid hormones on estradiol-induced vitellogenin (VTG) induction were electrophoretically examined in primary hepatocyte cultures of rainbow trout. Hepatocytes were precultured for 2 days and then estradiol-17 $\beta$ $(E_2,\;2 \times 10^{-6}M)$>, triiodothyronine $(T_3,\;10^{-8}-10^{-6}M)$, bovine growth hormone (bGH, 10-100 ng/ml), ovine prolactin (oPRL, 100-500 ng/ml), and pituitary extract (PE) of rainbow trout (0.75PE/dish) were added to the incubation medium. The hepatocytes were cultured for 7 more days. The addition of oPRL to the incubation medium was not effective in increasing VTG production at any concentrations. The addition of PE to the incubation medium with $E_2$ was not effective in increasing VTG production. The addition of bGH to the incubation medium with $E_2$ was not effective in increasing the rate of VTG production at concentrations of 10-50 ng/ml. However, a higher concentration of bGH, 100 ng/ml, increased VTG production. The various concentrations of $T_3$ were ineffective in stimulating VTG production. These results suggest that GH could be one of stimulus factors for VTG production in rainbow trout.