• Journal of Bio-Environment Control
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• v.24 no.1
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• pp.45-50
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• 2015

Edible sprouts are highly nutritious, containing numerous health-promoting phytochemicals and nutrients. However, the process of sprouting is subject to potential contamination by microorganisms attached to the seed coat. The objective of this study was to determine the effects of air anions generated by spray ionization on growth and sterilization of edible sprouts. Treatment with air anions has positive effects on plant growth and sterilization in 4 kinds of sprouts. Hypocotyl length in red cabbage and kale increased approximately 1.26-fold and radicle length of lettuce, red cabbage, and kale increased 1.4 to 1.6-fold compared to the control. The fresh weight of sprouts in the spray-ionization treatment was significantly (16.0-38.5%) higher than that of the controls. Radicle activity in sprouts exposed to anions was higher than that of the control in all species. Bacterial counts on lettuce, red cabbage, and tatsoi decreased significantly (by 41%, 66%, and 19%, respectively), and bacterial colonies also declined in drainage water. Our findings suggest that spray-ionization treatment is useful for improving sprout growth and for sterilizing sprouts.

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2008.10a
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• pp.82-84
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• 2008

• Journal of Bio-Environment Control
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• v.13 no.4
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• pp.200-208
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• 2004

Effects of cucumber and tomato seed germination by previous leaf and root vegetables (cabbage, radish, welsh onion, lettuce) residue in soil were tested in pot condition. Overall, suppression effect of welsh onion residue was the greatest in 4 tested crop residue and followed by radish, cabbage and lettuce, but lettuce residue didn't have effect on cucumber seed germination. Suppression were maintained ca. 20 days but after the time point, growth of cucumber and tomato were enhanced. Enhancing effect of welsh onion residue was the greatest in 4 tested crops residues and followed by radish, cabbage and lettuce. As a conclusion, residue of welsh onion, radish and cabbage were suppressed the germination of cucumber and tomato seeds but enhanced growth after 20 days of treatment. To verify the effective concentration of residue on suppression of germination and growth of radicle of cucumber and tomato, plant extract of welsh onion, radish, cabbage and lettuce were diluted as 5, 10, 17, 23, 35, 50, and $65\%$, and then tested. In low concentration treatment, 5, 10, 17, and $23\%$, all 4 crop extracts didn't suppress cucumber seed germination. High concentration of lettuce extract, 35, 50, and $65\%$, cucumber seed didn't germinate at all. In case of welsh onion extract, only $65\%$ treatment suppressed cucumber seed germination. In low concentration treatment, 17, and $23\%$, only the welsh onion extract suppressed young radicle. In case of high concentration treatment, except $35\%$, all four crops extract suppressed cucumber radicle growth. In low concentration treatment, tomato seed germination was suppressed by lettuce extract only but in high concentration treatment, 35, 50, and $65\%$, all extracts suppressed germination. Especially higher than $50\%$ treatment, tomato seed didn't germinate at all. Radicle growth was highly suppressed in welsh onion and lettuce extract, higher than $23\%$ concentration. As conclusion, leaf and root vegetable extracts suppressed cucumber and tomato seed germination and in high concentration, also suppressed radicle growth.

• Journal of Korean Society of Forest Science
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• v.81 no.1
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• pp.30-39
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• 1992

Artificial acid rain(pH2.0, 3.0, 4.0 and 5.0) was treated on the seeded pots of 4 species to examine its effects on germination, leaf injury and seedling growth. Aluminum ammonium sulfate solution (0.5, 1.0 and 2.5mM) and artificial acid rain (pH3.0, 4.0 and 5.0) were treated on the seeded germinators of 3 species to examine its effects on germination and radicle growth. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water(pH6.5) was used as control. Artificial acid rain was sprayed to seeded pots three times per week. About 5mm of artificial acid rain was treated each time from early April to early October, 1991. Germination, seedling establishment, radicle growth, leaf injury and seedling growth were measured and compared among the treatments. The results were summarized as follows 1. Germination, seedling establishment rate of Pinus densiflora were highest on the pH5.0 pot whereas those of Ailanthus altissima was highest on the control pot, and those of Magnolia sieboldii showed the highest value on the pH3.0 pot. 2. The differences in seedling height of Pinus densiflora and Pinus rigida were significant among the treatment levels of acid rain, whereas those of Magnolia sieboldii and Ailanthus altissima were not significant. Seedling heights of Pinus densiflora and Pinus rigida were highest on the pH3.0 pot, and that of Magnolia sieboldii was highest on the pH4.0 pot, and that of Ailanthus altissima was highest on the control pot. 3. Seedling dry weights of Pinus densiflora. Pinus rigida, and Ailanthus altissima differed significantly among the treatments, but that of Magnolia sieboldii did not differ. Highest seedling dry weights of Magnolia sieboldii and Ailanthus altissima were observed on the pH4.0 pot, and that of Pinus densiflora was observed on the pH3.0 pot, and that of Pinus rigida was observed on the pH5.0 pot. 4. With decrease of pH value of artificial acid rain, the number of injured needle and injured individual tended to be increased. 5. The differences in effects of soil acidity were highly significant among the treatments for all species. 6. Radicle growth of Pinus densiflora differed significantly among pH levels and aluminum concentrations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.137-141
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• 2006

Germination characteristics and alterations in soluble sugar-starch transition and phytic acid during germination were studied in rice seeds under saline conditions. NaCl significantly reduced the speed of germination. Also, the radicle growth out of seeds was severely inhibited by the exposure to NaCl solution, thus, seeds were almost impossible to grow to seedlings. Soluble sugar was remarkably accumulated, whereas starch was decomposed stepwise during seed germination. The metabolism of soluble sugar and starch in germinating seeds showed a distinct difference. The level of phytic acid in seeds decreased in all NaCl treatments during germination, but the level was affected differently by NaCl concentration in the two varieties. Overall, our results suggest that salt stress retard the radicle growth of rice seeds, and affect the starch-to-sugar conversion and the decomposition of phytic acid differently in two varieties.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.97-102
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• 2004

Paenibacillus polymyxa E681, with its plant growth promotion and root colonization ability, has been proven to be a promising biocontrol agent of cucumber and barley. This study investigated the attributes related to the movement of bacteria from the seed to the radicle and to the whole root system. It also illustrated the existing form and population changes of the bacteria on seed and root using the scanning electron microscope and confocal laser scanning microscopy. The bacteria invaded and colonized the inside of the seed coat while the seeds were soaked in bacterial suspension. Almost the same number of bacteria on seed surface invaded the inside of the seed coat right after seed soaking. The population densities of E681 increased greatly inside as well as on the surface of the seed before the radicle emerged. The bacteria attached on the emerging radicle directly affected the initial population of newly emerg-ing root. The colonized cells on the root were arranged linearly toward the elongation of the root axis. In addition to colonizing the root surface, strain E681 was found inside the roots, where cells colonized the inter-cellular space between certain epidermal and cortical cells. When the cucumber seeds were soaked in bacterial suspension and sown in pot, the bacterial populations attached on both the surface and inside of the root were sustained up to harvesting time. This means that E681 successfully colonized the root of cucumber and sustained its population density up to harvesting time through seed treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.526-534
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• 1996

This study was conducted to find out the effects of crude extracts from welsh onion, onion, chinese chives and garlic on the seedling growth of chrysanthemum, lettuce, rice, radish, chinese cabbage, cucumber and oriental melon. The crude extracts from Allium spp. inhibited the growth of chrysanthemum and lettuceseedlings at low concentration, the effect increased as the concentration grew higher, and especially in lettuce, the radicle growth was inhibited greater than the hypocotyl growth.The root extracts from welsh onion, chinese chives, and stem-leaf extracts from welsh onion, chinese chives and garlic significantly promoted the root growth of rice seedlings at 300∼700ppm and at 500∼1,000ppm, respectively, and the leaf sheath was elongated at low concentration of all extracts. The stem-leaf extracts from garlic and onion promoted the root and hypocotyl growth of chinese cabbage up to 2,000ppm or 3,000ppm. Root extracts from all Allium spp. promoted the elongation of chinese cabbage root up to 300∼700ppm, but they didn't influence its hypocotyl elongation. At low concentration of onion, garlic and chinese chives extracts, the growth of radish seedlings was highly promoted. The promotive effect, however, declined as the concentration increased. Only, extracts of chinese chives promoted the growth of radish seedling up to 5,000ppm. The stem-leaf extracts from onion and garlic promoted hypocotyl growth in pepper, up to 5,000ppm, but had little effect on other treatments. All of the extracts markedly promoted both root(main and lateral root) and hypocotyl growth in cucumber seedlings. A higher degree of promotion was made in the cucumber lateral root by onion and garlic extracts. The seedling growth of oriental melon was slightly increased by low concentration, but high concentration inhibited the root and hypocotyl growth.

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.329-340
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• 1996

The extracts of selected plants and analyzed phenolic compounds were used to study the effects of alleloKDICicals on seed germination and seedling growth. HPLC analysis of the aqueous extracts of seven species identified 15 phenolic compounds including caffeic acid. Among them, protocatechuic acid was detected at 65.87ppm and 6.84ppm, in Erigeron canadensis and Pinus rigida, respectively. And the extract of P. rigida showed the strongest inhibitory effect on seed germination. The extract of P. rigida leaves significantly inhibited germination and radicle growth of Raphanus sativus var. hortensis for. acanthiformis in direct proportion to concentration. However, germination of Cassia mimosoides var. nomame was stimulated by the treated extracts at the same concentrations, but root growth was inhibited at high concentrations. Except chlorogenic acid, eleven of the twelve phenolic compounds inhibited the germination of R. sativus var. hortensis for. acanthiformis. In the case of C. mimosoides var. nomame, some phenolic compounds such as chlorogenic acid, vanillic acid, protocatechuic acid, ferulic acid, gallic acid and ${\rho}-coumaric$ acid stimulated germination, while the others reduced it.

• Korean Journal Plant Pathology
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• v.1 no.3
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• pp.173-177
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• 1985

The objective of the study is to determine differences between cucurbits in the pathogenicity of Didymella bryoniae isolated from the naturally infected seeds of cucumber and pumpkin. Primary seedling infection of cucumber(Cucumis sativus L.), oriental melon(Cucumis melo var. makuwa Makino), pumpkin(Cucurbita pepo L.) and watermelon (Citrullus vulgaris Shrad.) occurred on the radicle, hypocotyl and cotyledons and symptoms on each crop were very similar. Infection of the radicle generally caused pre-emergence rot, while infection on the hypocotyl and cotyledons provided further inoculum for infection of the first true leaves and the stem. In cross inoculation tests, all isolates of D. bryoniae could infect cucumber, oriental melon, pumpkin and watermelon at different growth stages and there were not much differences in pathogenicity or susceptibility between isolates of the pathogen and crops tested. The susceptibility of cucumber and pumpkin was markedly influenced by prevailing humid conditions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.194-198
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• 1998

An experiment was carried out to find out the priming effects of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) on. the seedling establishment and early emergence under excess soil moisture conditions. Seeds were primed by soaking in -0.6 MPa polyethylene glycol (PEG) solution at $25^{\circ}C$ for 4 days. The primed seeds were sown in soils with various soil moistures (60, 80, 100, 120, and 140% field capacity) at 17 and $25^{\circ}C$, respectively. Germination and emergence rates, plumule height, and radicle length of primed seeds were higher than those of untreated seeds at any soil moisture and temperature examined. The time from planting to 50% germination ($T_{50}$) of primed seeds was less than that of untreated seeds by 0.9～3.7 days. Germination rate, emergence rate, plumule height, and radicle length were highest at the soil moisture of 80% field capacity among the soil moistures. Priming effects of rice seeds on germination and emergence rates were more prominent under the unfavorable soil moistures (60, 100, 120, and 140% field capacity) than those under the optimum soil moisture condition (80% field capacity). However, priming effects on seedling growth were greater at near optimum soil moisture compared with too lower or higher soil moistures. Therefore, these findings suggest that priming of rice seeds may be a useful way for better seedling establishment under the adverse soil conditions.