The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Colonization and Population Changes of a Biocontrol Agent, Paenibacillus polymyxa E681, in Seeds and Roots

  • Park, Okhee (Department of Agricultural Biology, Gyeongsang National University) ;
  • Kim, Jinwoo (Department of Agricultural Biology, Gyeongsang National University) ;
  • Ryu, Choong-Min (Department of Agricultural Biology, Gyeongsang National University) ;
  • Park, Chang-Seuk (Department of Agricultural Biology, Gyeongsang National University)
  • Published : 2004.06.01
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Abstract

Paenibacillus polymyxa E681, with its plant growth promotion and root colonization ability, has been proven to be a promising biocontrol agent of cucumber and barley. This study investigated the attributes related to the movement of bacteria from the seed to the radicle and to the whole root system. It also illustrated the existing form and population changes of the bacteria on seed and root using the scanning electron microscope and confocal laser scanning microscopy. The bacteria invaded and colonized the inside of the seed coat while the seeds were soaked in bacterial suspension. Almost the same number of bacteria on seed surface invaded the inside of the seed coat right after seed soaking. The population densities of E681 increased greatly inside as well as on the surface of the seed before the radicle emerged. The bacteria attached on the emerging radicle directly affected the initial population of newly emerg-ing root. The colonized cells on the root were arranged linearly toward the elongation of the root axis. In addition to colonizing the root surface, strain E681 was found inside the roots, where cells colonized the inter-cellular space between certain epidermal and cortical cells. When the cucumber seeds were soaked in bacterial suspension and sown in pot, the bacterial populations attached on both the surface and inside of the root were sustained up to harvesting time. This means that E681 successfully colonized the root of cucumber and sustained its population density up to harvesting time through seed treatment.

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References

  1. Ahmad, J. S. and Baker, R. 1987. Rhizosphere competence in Trichoderma harzianum. Phytopathology 77: 192-189
  2. Bae, Y. S., Kim, H. K. and Park C. S. 1990. An improved method for rapid screening and analysis of root colonizing biocontrol agents. Korean J. Plant Pathol. 6:325-332
  3. Bull, C. T., Weller, D. W. and Thomashow, L. S. 1991. Relationship between root colonization and suppression of Gaeumannomyces graminis var. tritici by Pseudomonas jluorescens strain 2-79. Phytopathology 81 :954-959 https://doi.org/10.1094/Phyto-81-954
  4. Caesar, A. J. and Burr, T. J. 1991. Effect of conditioning, betaine and sucrose on survival of rhizobacteria in power formulations. Appl. Environ. Microbiol. 57: 168-172
  5. Chao, W. L., Nelson, E. B., Harman, G. E. and Hoch, H. C. 1986. Colonization of rhizosphere by biological control agents applied to seeds. Phytopathology 76:60-65 https://doi.org/10.1094/Phyto-76-60
  6. Chin-A-Woeng, T. E., de Priester, W, van der Bij, A. J. and Lugtenberg, B. J. 1997. Description of the colonization of a gnotobiotic tomato rhizosphere by Pseudomonas jluorescens biocontrol Strain WCS365, using scanning electron microscopy. Mol. Plant-Microbe Interact. 10:79-86 https://doi.org/10.1094/MPMI.1997.10.1.79
  7. Glick, B. R, Changping, L., Sibdas, G. and Dumbroff, E. B. 1997. Early development of canola seedlings in the presence of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2. Soil BioI. Biochem. 29: 1233-1239 https://doi.org/10.1016/S0038-0717(97)00026-6
  8. Hallmann, J., Quadt-Hallmann. A., Mahaffee, W. E and Kloepper, J. W. 1997. Bacterial endophytes in agricultural crops. Can. J. Microhiol. 43:895-914 https://doi.org/10.1139/m97-131
  9. Hood, M. A., van Diik, K. V. and Nelson, E. B. 1998. Factor affecting attachment of Enterohacter cloacae to germinating cotton seed. Microb. Ecol. 36:101-110 https://doi.org/10.1007/s002489900097
  10. Howie, W. J., Cook, R. J. and Weller, D. M. 1987. Effects of soil matric potential and cell motility on wheat root colonization by fluorescent pseudomonads suppressive to take-all. Phytopathology 77:286-292 https://doi.org/10.1094/Phyto-77-286
  11. Kang,J. H. and Park, C. S. 1997. Colonization pattern of fluorescent pseudomonads on the cucumber seed and rhizoplane. Korean J. Plant Pathol. 13:160-166
  12. Kloepper, J. W. and Schroth, M. N. 1978. Plant Growth-Promoting Rhizobacteria on radishes. pp. 879-882 In: Proc. Int. Cont. Plant Pathog. Bact. 4th. vol. 2
  13. Loper, J. E., Haach, C. and Schroth, M. N. 1985. Population dynamics of soil Pseudomonas in the rhizosphere of potato (Solanum tuberosum L). Appl. Environ. Microbiol. 49:416-422
  14. Ryu, C. M. and Park, C. S. 1997. Enhancement of plant growth induced by endospore forming PGPR strain, Paenibacillus polymyxa E681. Proc. PGPR workshop. pp. 186-190. Sapporo, Japan
  15. Shah-smith, D. A. and Bums, R. G. 1996. Biological control of damping-off of sugar beet by Pseudomonas putida applied to seed pellets. Plant pathology 45:572-582
  16. Weller, D. M. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Ann. Rev. Phytopathology 26:379-407. https://doi.org/10.1146/annurev.py.26.090188.002115
  17. Xi, K., Stephens,J. H. and Verma, P. R. 1996. Application of formulatedrhizobacteria against root rot of field pea. Plant Pathology 45: 1150-1158 https://doi.org/10.1046/j.1365-3059.1996.d01-188.x

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