• Fisheries and Aquatic Sciences
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• 제11권4호
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• pp.183-189
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• 2008

In this study, tests were conducted to investigate the effects of three dietary growth hormones, administered in various amounts, on the growth performance and lysozyme activity in juvenile olive flounder, Paralichthys olivaceus. Three dietary growth hormones, recombinant human growth hormone (rHGH), recombinant bovine somatotropin A (rBST A) and recombinant bovine somatotropin B (rBST B) were tested at three different supplemental levels (10, 20 or 40 mg/kg body weight per week) by a $3{\times}3$ factorial design and a complete randomized design in comparison to a control group. Fish were fed one of the ten experimental diets (control, $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{10}$, rBST $B_{20}$ and rBST $B_{40}$) for 6 weeks and afterward were analyzed for growth performance by measuring weight gain (WG), feed efficiency (FE), specific growth rate (SGR) and protein efficiency ratio (PER). Based on the factorial design analysis, fish fed rHGH diets demonstrated significantly higher growth performance than fish fed rBST A or rBST B diets. However there were no significant differences in WG, FE, SGR and PER between fish fed rBST A and rBST B diets. Neither hormone level nor the interaction between the different hormones and their various levels had a significant effect on WG, FE, SGR, PER, lysozyme activity or whole-body proximate composition. A complete randomized design analysis confirmed fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{20}$ and rBST $B_{40}$ diets for 6 weeks showed higher WG than fish fed the control diet (P<0.05). A higher FE was observed in fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{20}$ and rBST $A_{40}$ diets in comparison to fish fed the control diet. Fish fed all graded rHGH, rBST A and rBST B supplemented diets showed a higher SGR than fish fed the control diet. Regarding PER, fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$ and rBST $B_{20}$ diets were higher than fish fed the control diet. Furthermore, the lysozyme activity of fish fed a diet of $rHGH_{20}$ was significantly higher than that of fish fed any other diet. The results measuring the growth and development of the fish clearly suggest the biopotency of dietary rHGH could be higher than those of both dietary rBST A and rBST B. Further implied is the probability that within the range of 10 to 40 mg/kg BW/week the dietary growth hormones could accelerate growth performance, and that 20 mg rHGH/kg BW/week could possibly enhance lysozyme activity in juvenile olive flounder, Paralichthys olivaceus.

• 한국가축번식학회지
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• 제24권2호
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• pp.163-170
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• 2000

본 연구는 rBST, vitamin E 및 selenium (Se)의 투여가 한우 종모우의 혈액성분 및 호르몬의 농도변화에 미치는 영향을 검토하였다. 1. rBST 투여구, Vit. E 투여구 및 Se 투여가 한우 종모우의 혈액성분에 미치는 효과를 조사한 결과, 혈중 albumin의 함량은 Se 투여구가 대조구, rBST 투여구 및 Vit. E 투여구보다 통계적으로 유의하게 높은 함량을 나타났으며 (P＜0.05), 혼합투여구는 여타구 (대조구, rBST 투여구 및 vitamin E 투여구)보다 통계적으로 유의하게 높게 나타났다 (P＜0.05). 혈중 BUN (Blood Urea Nitrogen)과 creatinine의 함량은 rBST 투여구가 여타구보다 통계적으로 유의하게 높게 나타났다 (P＜0.05). 혈중 total protein 함량은 rBST 투여구, Se 투여구 및 혼합투여구가 대조구보다 통계적으로 유의하게 높은 함량을 나타냈다 (P＜0.05). 혈중 calcium, cholesterol, glucose, inorganic phosphoros 의 함량은 투여구간에 차이가 없었다 (P＞0.05). 2. rBST, Vit. E 및 Se 투여가 한우 종모우의 혈중내 호르몬의 변화에 미치는 효과를 조사한 결과, 혈중 estradiol의 농도는 Se 투여구와 혼합투여구가 여타구 (대조구, rBST 투여구 및 Vit. E 투여구 )보다 다소 높게 나타났지만 유의차는 인정되지 않았으며 (P＞0.05), 혈중 testeserone의 농도는 투여구간에 커다란 차이는 없었다 (P＞0.05).

• 한국임상수의학회지
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• 제16권2호
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• pp.332-338
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• 1999

This study was carried out to investigate weight gain and prevention of diarrhea in suckling calves which were injected with recombinant bovine somatopropin (rBST). A total of 101 breeding cows were assigned to the six groups according to the administered dosage and injected time, respectively. Groups T-1, T-3, T-4 and T-6 were injected starting 1 week before calving and groups T-2 and T-5 were injected on the calving day. The six groups were injected five times at two week intervals. Groups T-1 and T-2 were injected with 250mg composite rBST, Group T-3 was injected with 375 mg composite rBST. Groups T-4 and T-5 were injected with 500mg composite rBST, And group T-6 was injected with 500mg rBST-S. The control group was not injected with BST. The groups injected with 500 mg BrST had a lower rate of morbidity from diarrhea than the control group or the groups injected with 250 mg rBST (T-1 and T-2). Weight gain was higher in group T-4 than in the control group or groups T-1 and T-2. In Korean Native Cattle, the total weight gain was greater in group T-4 than in the control group (p<0.05). In crossbred cows, total weight gain was the highest in group T-4, and the total weight gain rate was greater than in group T-4 and the control group (p<0.05). The results of the hematological values showed that injections of rBST did not affect the level of the RBC, TP and BUN in the breeding cows at 9 weeks after postpartum or the neonatal calves. The results of this study indicate that injecting breeding cows with 500 mg rBST before calving would be effective in the preventing of diarrhea and in increasing weight gain of calves from birth to weaning.

• 한국임상수의학회지
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• 제19권1호
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• pp.43-48
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• 2002

This study was performed to investigate the effects of recombinant BST formulation treatment on the milk yield, milk components, mastitis, and general cow health condition when the formulations of retinyl palmitate, cholecalciferol and rBST were administered after the peak period of milk production. The milk yields of treatment groups (Group I, II, III and IV) were increased from 21.5% to 29.0% than that of control group. There was significant difference in milk production between treatment group II, IV and control group (P<0.05). However, there was no significant difference in milk production between treatment group I, III and control group (P<0.05). And the addition of retinyl palmitate and cholecalciferol into rBST formulation did not increase the milk yield. The milk of treatment groups with sustained-release rBST did not show significant difference in milk components (milk fat, protein, lactose, and solid not fat). However, there were minor changes, primarily in fat content of milk, during the first few weeks of rBST administration. There was no incidence of clinical mastitis between rBST treatment groups and control group. Addition of high and medium concentration of retinyl palmitate and cholecalciferol into sustained-release rBST formulation was efficient in reduction of somatic cell count in milk. There was great energy deficit in all treatment groups compared with control group during the early study period. Thus, the body condition score of all treatment groups showed lower value than control group. No evidences of metabolic health problems, such as ketosis, milk fever, and downer cow were observed. Incidence of general lameness did not appear on all treatment groups during 140 days of this study.

• 한국가축번식학회지
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• 제20권2호
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• pp.197-205
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• 1996

This study was conducted to examine the effect of sustained release recombinant bovine somatotropin(SR-rBST) with different dosage(0, 0.03mg/kg body weight/14 day, 0.06mg/kg body weight/14 day, 0.09mg/kg body weight/14 day) on the economical traits of the semen characteristics and sexual behaviors and blood chemical values with sixteen Holstein dairy young bulls in Dairy Cattle Improvement Center of National Livestock Co-operatives Federation. Sensual testings of collected semens of Holstein young bulls were not different among treatments (p>0.05). Ejaculated semen volumes of control, SR-rBST 0.03mg, 0.06mg and 0.09mg groups were 5.6ml, 5.6ml, 6.0ml and 6.7m, respectively, but the result of SR-rBST 0.09mg group significantly increased semen volume(P<0.05). In sperm concentration, SR-rBST 0.06mg and 0.09mg groups significantly increased the total number of sperms than the other groups(P<0.05). In otherwise, SR-rBST treatments did not affect on pH, osmotic pressure, anti forzen rate, abnormality and motility of collected semen during whole experimental period. Reaction time(RT), sexual aggressiveness(SA) and tactile stimulation(TS) were not different among treatments. The libido scores(LS) of control, 0.03mg, 0.06mg and 0.09mg groups were 68.0, 80.5, 73.9 and 80.8, respectively, and LS were significantly improved by SR-rBST administration(P<0.05). The effect of SR-rBST on scrotal circumference measurement that is important factor to determine the ability of semen production, was not different among treatments. These studies indicates that SR-rBST treatments favourably affect on semen quality and sexual libido in Holstein young bull.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집
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• pp.73-76
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• 2001

We deposited epitaxial $Ba_{0.6}$S $r_{0.4}$Ti $O_3$(BST) films having thickness of 400 nm on MgO(001) substrates, where a 10 nm thick $Ba_{1-x}$S $r_{x}$Ti $O_3$(x=0.1-0.7) interlayer was inserted between BST and MgO to manipulate the stress of the BST films. Since the main difference of those epitaxial BST films was the lattice constant of the interlayers, we were very successful in controlling the stress of the BST films. BST films under small tensile stress showed larger dielectric constant than that without stress as well as those under compressive stress. Stress relaxation was investigated using epitaxial BST films with various thicknesses grown on different interlayers. For BST films grown on $Ba_{0.7}$S $r_{0.3}$Ti $O_3$ interlayers, the critical thickness was about 600 nm. On the other hand, the critical thickness of single-layer BST film was less than 100 nm.00 nm.m.m.m.

• 한국세라믹학회지
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• 제40권2호
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• pp.198-205
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• 2003

R.F. Magnetron Sputtering법을 이용하여 Pt/Ti/ $SiO_2$/Si기판 위에 seed-layers와 $Ba_{0.66}$S $r_{0.34}$Ti $O_3$박막을 제조하였다. 다양한 기판온도에 따른 BST 박막의 전기적인 특성(정전용량과 누설전류)과 seed-layer층이 BST 박막에 미치는 영향을 조사하였다. BST 박막은 seed-layer층을 삽입함으로써 박막의 결정성이 향상되었고, 박막의 기판온도(결정화온도)도 상당히 낮출 수 있었다. 순수한 BST에 비하여 seed-layer를 삽입한 BST는 높은 유전상수와 낮은 유전손실 및 낮은 누설전류를 가지는 우수한 전기적 특성을 나타내었다. BST 박막의 전기적 특성은 기판온도에 따라 영향을 받고, seed-layer에 의해 향상됨을 알 수 있었다.

• 한국가축번식학회지
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• 제23권3호
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• pp.205-212
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• 1999

본 연구는 rBST, Vit. E 및 Se의 투여가 한우 종모우의 혈청내 Se과 vitamin E의 농도 변화에 미치는 영양을 검토하였다. 1. rBST, Vit. E 및 Se 투여가 한우 종모우의 혈청 내 Se농도 변화에 미치는 영향을 조사한 결과, 혈청내 Se 농도는 Vit. E와 Se 혼합투여구가74.09ppb/ml로서 대조구(64.55ppb/ml)와 rBST투여구(65.50ppb/ml) 및 vit. E 투여구(68.15ppb/m1)보다 높은 농도를 나타냈으나(P<0.05), selenium 투여구(73.11ppb/ml)와는 통계적 유의차가 없었다(P>0.05). 2. rBST, Vit. E 및 Se 투여가 한우 종모우의 혈청 내 Vit. E 농도 변화에 미치는 영향을 조사한 결과, 혈청내 Vit. E농도는 Vit. E투여구와 혼합투여구가 각각 2.80 및 2.75ppm/ml로서 여타구(대조구, 2.27 ; rBST투여구, 2.32 및 Se투여구, 2.58ppm/rnl)보다 다소 높게 나타났지만 통계적 유의차는 인정되지 않았다(P>0.05). 3. Vt. E 투여구, Se 투여구 및 혼합투여구에서 투여 횟수에 따른 혈청내 Se과 Vit. E의 농도 변화는 모든 투여구에서 투여 횟수가 증가함에 따라 혈청내 농도가 증가하였다.

• 한국가축번식학회지
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• 제23권3호
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• pp.191-203
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• 1999

본 연구는 rBST, Vito E 및 Se 투여가 한우 종모우의 정액성상에 미치는 효과를 검토하였고 정액의 동결보존 시 정액희석액에 taurine의 첨가가 융해 후 정액성상에 미치는 영향을 검토하였다. 1. rBST, Vit. E, Se 및 Vit. E와 Se 혼합 투여가 한우 종모우의 정액량, 정자농도 및 수소이온농도 (pH)에 미치는 영향을 조사한 결과, 정액량과 수소이온농도는 처리구간에 커다란 차이가 없었으며(P＜0.05), 정자의 평균농도는 각각 13.50$\pm$2.32, 16.26$\pm$2.65, 17.07$\pm$2.61, 19.23$\pm$2.07 및 19.46$\pm$2.06$\times$$10^{8}$ 정자/$m\ell$로서 Se 투여구와 혼합투여구가 대조구, rBST 투여구 및 Vit. E 투여구보다 통계적으로 유의하게 높은 정자농도를 나타냈다 (P＜0.05). 2. rBST. Vit. E 및 Se 투여가 한우 종모우의 정액의 동결융해시 정액성상에 미치는 영향을 조사한 결과, 정자의 운동율과 기형율은 투여구간에 통계적 유의차는 없었으나, 정자의 생존율은 혼합투여구가 48.24%로서 대조구 (44.48%)보다 통계적으로 유의하게 높은 생존율을 나타냈으며 (P＜0.05), rBST 투여구, Vit. E 투여구 및 Se 투여구는 각각 46.48%, 47.28% 및 46.34%로서 대조구와 통계적 유의차는 인정되지 않았다. 3. 정액의 동결보존시 정액회석액에 taurine의 첨가가 정액의 융해 후 정액성상에 미치는 영향을 조사한 결과는 총정자수, 운동율, 생존율 및 기형율에서 taurine 첨가구가 taurine 무첨가구보다 다소 좋은 결과를 얻었지만 커다란 차이가 없었다. 4. rBST, Vit. E 및 Se 투여가 정자의 수정능획득과 첨체반응에 미치는 효과를 조사한 결과, 수정능 획득과 침체반응이 모두 일어나지 않는 비율(F율)과 모두 일어난 비율 (AR율) 은 투여구간에 커다란 차이가 없었다 (P＜0.05). 5. 정액의 동결보존 시 taurine 의 첨가가 융해후 정자의 수정능획득과 첨체반응에 미치는 영향을 조사한 결과는 수정능 획득이 일어났지만 첨체 반응이 일어나지 않은 비율 (B율)과 AR 율에서는 투여구간에 커다란 차이가 없었지만, F율은 taurine 첨가구가 taurine 무첨가구보다 낮은 비율을 나타났다 (P＜0.05).

• Journal of Animal Science and Technology
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• 제47권3호
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• pp.439-446
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• 2005

This study was conducted to elucidate the influence of recombinant bovine somatotropin (rBST) on the plasma IGF-I profiles in deer stags. Slow release rBST (250mg each) preparations were injected subcutaneously during the antler's non-growing period and growing period. It was observed that significant increases in the plasma IGF-I concentrations were occurred right after injections of the rBST irrespective of antler's non-growing period or growing period. When the rBST preparations were injected two times with one week interval during the antler's non-growing period, the mean level of IGF-I was found to be 2,920.7ng I mL, indicating that the IGF-I concentration increased up to 8.4-folds compared with a control. After two weeks from second shot, however, the IGF-I levels decreased to the level of the control. Similar results were also obtained with both a Sika and a Red deer during the antler's growing period. Increases in the IGF-I level of up to 5.6-folds with the Sika deer and 4.3-folds with the Red deer were found. Based on the present study, it is likely that the rBST may be used as a possible candidate for an antler's growth stimulating agent.