• Title/Summary/Keyword: quality grades

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Regional Differences of Proteins Expressing in Adipose Depots Isolated from Cows, Steers and Bulls as Identified by a Proteomic Approach

  • Cho, Jin Hyoung;Jeong, Jin Young;Lee, Ra Ham;Park, Mi Na;Kim, Seok-Ho;Park, Seon-Min;Shin, Jae-Cheon;Jeon, Young-Joo;Shim, Jung-Hyun;Choi, Nag-Jin;Seo, Kang Seok;Cho, Young Sik;Kim, MinSeok S.;Ko, Sungho;Seo, Jae-Min;Lee, Seung-Youp;Chae, Jung-Il;Lee, Hyun-Jeong
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.8
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    • pp.1197-1206
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    • 2016
  • Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis. Two different adipose depots (i.e. intramuscular and omental) were collected from cows (n = 7), steers (n = 7), or bulls (n = 7). LC-MS/MS revealed a total of 55 and 35 proteins in IMAT and OMAT, respectively. Of the 55 proteins identified, 44, 40, and 42 proteins were confirmed to be differentially expressed in IMAT of cows, steers, and bulls, respectively. In OMAT of cows, steers, and bulls, 33, 33, and 22 were confirmed to be differentially expressed, respectively. Tropomyosin (TPM) 1, TPM 2, and TPM3 were subjected to verification by quantitative PCR and western blot analysis in IMAT and OMAT of Hanwoo cows, steers, and bulls as key factors closely associated with muscle development. Both mRNA levels and protein levels of TPM1, TPM2, and TPM3 in IMAT were lower in bulls compared to in cows or steers suggesting that they were positively correlated with marbling score and quality grade. Our results may aid the regulation of marbling development and improvement of meat quality grades in beef cattle.

Quality Comparison of Chuncheon Dakgalbi Made from Korean Native Chickens and Broilers (토종닭과 육계로 만든 춘천닭갈비의 품질비교)

  • Lee, Sung-Ki;Choi, Won-Hee;Muhlisin, Muhlisin;Kang, Sun-Moon;Kim, Cheon-Jei;Ahn, Byoung-Ki;Kang, Chang-Won
    • Food Science of Animal Resources
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    • v.31 no.5
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    • pp.731-740
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    • 2011
  • This study was conducted to evaluate a quality comparison between Chuncheon Dakgalbi made from Korean native chickens (KNC) and that made from commercial broilers. Two Korean native chickens including Woorimatdag (KNCWoori) and Hanhyup3 (KNC-Hanhyup), and two commercial broilers including grades of 18 (Broiler-18) and 13 (Broiler-13) were slaughtered at 110, 70, 38, and 31 d of ages. Chuncheon dalkalbi was prepared by mixing/dipping the meat in chili pepper sauce; it was then packed with air-packaging (Air-P) and 30% $CO_2$-MAP (0% $O_2$/30% $CO_2$/70% $N_2$), and stored at $5^{\circ}C$ for 10 d. The results showed that the KNC group had a lower pH but a higher cooking loss compared with the broiler group (p<0.05). In a texture analysis, KNC-Woori had the highest shear force value among the breeds (p<0.05). For the fatty acid composition of the thigh, the KNC-Woori contained more total saturated acids, myristic acid, palmitic acid and stearic acid, but less total unsaturated fatty acids, linoleic acid and linolenic acid than other breeds (p<0.05). Also, the n6/n3 ratios of the KNC group (19.24 and 16.77) were higher than those of the broiler group (14.02 and 14.77) (p<0.05). The total acceptability scores of Dakgalbi made from the KNC group were decreased by sensory panelists. The Dakgalbi with 30% $CO_2$-MAP delayed the protein deterioration (Volatile basic nitrogen) and lipid oxidation during storage. However, no clear evidence was observed of $CO_2$-MAP on the effect of different chicken materials. It is suggested that 30% $CO_2$-MAP instead of Air-P is used for methods for Chuncheon Dakgalbi. Furthermore, it might be unfavorable to use Korean native chickens as raw material for Chuncheon Dakgalbi from a practical quality point of view.

Transfer and Validation of NIRS Calibration Models for Evaluating Forage Quality in Italian Ryegrass Silages (이탈리안 라이그라스 사일리지의 품질평가를 위한 근적외선분광 (NIRS) 검량식의 이설 및 검증)

  • Cho, Kyu Chae;Park, Hyung Soo;Lee, Sang Hoon;Choi, Jin Hyeok;Seo, Sung;Choi, Gi Jun
    • Journal of Animal Environmental Science
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    • v.18 no.sup
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    • pp.81-90
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    • 2012
  • This study was evaluated high end research grade Near infrared spectrophotometer (NIRS) to low end popular field grade multiple Near infrared spectrophotometer (NIRS) for rapid analysis at forage quality at sight with 241 samples of Italian ryegrass silage during 3 years collected whole country for evaluate accuracy and precision between instruments. Firstly collected and build database high end research grade NIRS using with Unity Scientific Model 2500X (650 nm~2,500 nm) then trim and fit to low end popular field grade NIRS with Unity Scientific Model 1400 (1,400 nm~2,400 nm) then build and create calibration, transfer calibration with special transfer algorithm. The result between instruments was 0.000%~0.343% differences, rapidly analysis for chemical constituents, NDF, ADF, and crude protein, crude ash and fermentation parameter such as moisture, pH and lactic acid, finally forage quality parameter, TDN, DMI, RFV within 5 minutes at sight and the result equivalent with laboratory data. Nevertheless during 3 years collected samples for build calibration was organic samples that make differentiate by local or yearly bases etc. This strongly suggest population evaluation technique needed and constantly update calibration and maintenance calibration to proper handling database accumulation and spread out by knowledgable control laboratory analysis and reflect calibration update such as powerful control center needed for long lasting usage of forage analysis with NIRS at sight. Especially the agriculture products such as forage will continuously changes that made easily find out the changes and update routinely, if not near future NIRS was worthless due to those changes. Many research related NIRS was shortly study not long term study that made not well using NIRS, so the system needed check simple and instantly using with local language supported signal methods Global Distance (GD) and Neighbour Distance (ND) algorithm. Finally the multiple popular field grades instruments should be the same results not only between research grade instruments but also between multiple popular field grade instruments that needed easily transfer calibration and maintenance between instruments via internet networking techniques.

Evaluation of Image Quality for 2D TSE(RT) and 3D GRASE in MRCP Study: Fast MRCP Method (췌담관자기공명영상에서 2D TSE(RT)와 3D GRASE(BH) 기법에 대한 영상의 질 평가 : Fast MRCP 기법)

  • Goo, Eun-Hoe
    • Journal of the Korean Society of Radiology
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    • v.15 no.7
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    • pp.983-989
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    • 2021
  • In this study, we intend to evaluate image quality and provide to clinical basic data by applying to 2D TSE (RT) and 3D GRASE (BH) techniques using Fast MRCP testing methods for application to patients in poor patient condition. Data were analyzed for 30 patients (15 males, 15 females, and 64±4.26 average age) who underwent MRCP tests. The equipment used was Ingenia CX 3.0 T equipment and Ds anterior coil was used for data acquisition. SNR and CNR of each image were measured through quantitative analysis, and the quality of the image was evaluated by dividing it into 5 grades for qualitative evaluation. The image evaluation was performed on the paired t-test and the Wilcoxon test, and when the p value was 0.05 or less, it was considered to be significant. As a result of quantitative analysis of SNR and CNR, 3D GRASE (BH) was measured high when comparing the two techniques, 2D TSE (RT) MRCP and 3D GRASE (BH) (p<0.05). The qualitative analysis result is a sharpness of the bile duct: 3D GRASE(BH): 4.12±0.03, Overall image quality: 3D GRASE(BH): 4.21±0.91 was high (p=0.001). The motion artifact of the bile duct showed no significant difference with two techniques(2D TSE(RT): 4.41±0.04, 3D GRASE(BH): 4.53±0.14(p=0.067). However, the background suppression obtained significant results with 2D TSE(RT) of 4.14±0.55(p=0.001). In conclusion, as a result of using the Fast MRCP testing method, MRCP images obtained by 3D GRASE (BH) had an advantage over MRCP images using 2D TSE (RT). However, there will be useful results of 2D TSE(RT) MRCP technique in patients who have difficulty holding their breath.

A Study on the Construction Methods and the Distribution of Proper Spatial Function for Restoring Urban Streams into Close-to-Nature Streams - A Case Study of Hongjecheon(Stream) in Seodaemun-Gu, Seoul - (도시 내 자연형 하천 조성을 위한 적정 공간기능 배분과 조성방안 연구 - 서울시 서대문구 홍제천을 사례로 -)

  • Jung, Tae-Jun;Lee, Kyong-Jae;Han, Bong-Ho
    • Journal of the Korean Institute of Landscape Architecture
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    • v.41 no.3
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    • pp.43-55
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    • 2013
  • The purpose of this study is to propose a plan that properly organizes urban close-to-nature streams by evaluating the city park functions, ecological functions and landscape functions required for urban stream and setting up space function suitable for the space. The site of this study is Hongjecheon located in Seodaemun gu of Seoul City, whose length of channel spans 6.12km in total. The plan for the construction of close-to-nature streams had been established from late 2003, and the construction was completed. Evaluation Categories and indications were deduced from 4 stages. First, based on theoretical examination, we made a list of stream and park evaluation categories and added Category about Characteristic of urban streams. Next, we set Final Evaluation Categories and indications through the process of goal-relevance, indication verification, merging similar category. Final Evaluation Categories were deduced such as usage demand, usability(city park functions), biodiversity, inhabitation potential, rarity(ecological functions), historical cultural elements, and landscape Quality(landscape functions). As a result of allotting space functions, zones 1 through 4, got high grades at usage demand, was classified as a civic resort district; zones 5 through 6, close to major green area and remained original landscape, as ecological conservation and restoration district; zones 7 through 8, get high grades at usage demand and usability, as environmentally-friendly use district; and zones 9 through 10, many historical cultural elements and view points, and high green possession rate, as stream scenic district. In addition, detail space function and construction plan for each zones were proposed. As a result of this study, proposed space function assignment considering natural characteristics, humanities and social characteristics and landscape characteristics and is expected to be utilized at reasonable spatial planning considering various functions required for urban stream.

Effect of Transportation Distance of Broilers on Meat Grades and PSE Incidence (육계 출하 시 수송 거리가 닭고기의 도체 등급 및 PSE육 출현에 미치는 영향)

  • Chae, H.S.;Yoo, Y.M.;Jeong, S.G.;Ham, J.S.;Ahn, C.N.;Kim, D.H.;Jang, A.
    • Korean Journal of Poultry Science
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    • v.36 no.1
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    • pp.9-14
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    • 2009
  • This study was conducted to evaluate the effect of three types of transportation distance (short: less than 40 km; intermediate: 70~80 km; long: 140~150 km) on quality of whole chicken and chicken meat cuts. In whole chicken, $1^+$ grade chicken incidence after transportation of short, intermediate, and long distance transportation was 61.3, 56.3, and 43.8%, respectively. Bruise was not shown in breast and thigh, while wings with bruise after short and long distance transportation were 16.7% and 27.3%, respectively. For chicken meat cuts, $1^+$ grade chicken breast incidence after short distance transportation was 14% higher than that after long distance transportation. Bruised chicken breast after short and long distance transportation were 10 and 12%. Pale, soft, and exudative (PSE) chicken breast incidence after long distance transportation was higher (18.3%) than that after short distance transportation (7.0%). Chicken thigh cuts with $1^+$ grade after short and long distance transportation were shown 91.2% and 88.3%, respectively. Also, chicken wing cuts showed similar result to chicken thigh cuts' and $1^+$ grade incidence of chicken wings after short distance transportation was 11% higher than that of after long distance transportation. These results suggest that shorter transportation distance from farm to slaughter house result in high grade whole chicken and chicken meat cuts.

Determination of Grades and Design Strengths of Machine Graded Lumber in Korea (국내 기계등급구조재의 등급구분체계 및 기준설계값 결정방법 연구)

  • Hong, Jung-Pyo;Lee, Jun-Jae;Park, Moon-Jae;Yeo, Hwanmyeong;Pang, Sung-Jun;Kim, Chul-Ki;Oh, Jung-Kwon
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.4
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    • pp.446-455
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    • 2015
  • Based on comparative studies on standards and grading procedures of machine graded lumber in Korea and other countries, this study proposed a procedure of determining the grade classification and design strengths of domestic machine graded lumber. Differences between machine stress rated lumber and E-rated laminations were detailed in order to clarify the need for the procedure improvement. To this improvement the use of average MOE requirement for grading was introduced instead of the fixed minimum MOE requirement which is currently used in the Korean standards. It was found that the fixed minimum MOE requirement method was easier for an inspector to grade but, less efficient as a strength predictor than the average MOE requirement method. The advantage of average MOE requirement method is statistically MOR-MOE regression-based MOR prediction and highly efficient in quality control though it requires a computer-aided operation system in an initial setup. A major weakness of the current Korean grading system was found that different strength characteristics depending on wood species were not reflected on the grade classification and the tabulated allowable design stress. The proposed procedures were developed taking advantages of respective merits of both methods and based on MOR-MOE regression analysis. Through this procedure, the grades of machine stress rated lumber should be revised to become interchangeable with E-rated lamination, which would be beneficial to the cost competitiveness of domestic machine graded lumber and glued laminated timber industry.

Effects of Dietary Buffer Material for Chicken Meat Grades and PSE Incidence in Broilers under Transport Heat Stress (고온기 육계의 수송 스트레스에 대한 버퍼제 급여에 따른 닭고기 품질 및 PSE 발생에 미치는 영향)

  • Chae, Hyun-Seok;Choi, Hee-Chul;Na, Jae-Cheon;Jang, Ae-Ra;Kim, Min-Ji;Bang, Han-Tae;Kang, Hwan-Ku;Kim, Dong-Wook;Seo, Ok-Suk;Park, Sung-Bok;Ham, Jun-Sang
    • Korean Journal of Poultry Science
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    • v.37 no.2
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    • pp.131-137
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    • 2010
  • The present study has been performed to suggest a method to decrease the adverse effects of transportation on chicken meat quality. The groups were prepared as follows; Control group and three groups of treatments (sodium phosphate, sodium bicarbonate and magnesium sulfate). The chicken fed magnesium sulfate showed higher chicken meat quality compared to control and other treatment groups. Also, minor and severe PSE incidence of chicken breast was found at 88% in sodium phosphate group, 24% in sodium bicarbonate group and 56% in magnesium sulfate group. Control group showed 92% higher minor and severe PSE incidence of chicken breast compared to other groups. In control group, the external bruise of chicken showed 32% but 22, 24 and 44% in other treatment groups, respectively. Lightness ($L^*$) of chicken containing sodium phosphate treatment, sodium bicarbonate treatment and magnesium sulfate were 67.05, 66.27 and 65.89, while Lightness ($L^*$) of chicken containing control group was decreased of 67.88. In conclusion, dietary buffer material (sodium phosphate, sodium bicarbonate, magnesium sulfate) under heat stress decreased adverse effects including death, wound or abnormality of chickens.

International Comparison Study on the Articulation of the Science Curriculum: Focus on the Concept of Photosynthesis (과학과 교육과정의 연계성 국제 비교: 광합성 개념 중심으로)

  • Lee, Hyonyong;Yeo, Chaeyeong
    • Journal of The Korean Association For Science Education
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    • v.35 no.5
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    • pp.805-815
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    • 2015
  • The Korean education curriculum is making efforts to improve education to foster competencies that the future society demands through the 2007 and 2009 revised curriculum. The revised curricula focus on enhanced articulation for the quality curriculum. In this study, the curriculum is analyzed for vertical and horizontal articulation. In addition, the study found a problem in Korea's curriculum through international comparison and sought improvement. Furthermore, the study compared internationally articulation of the concept of photosynthesis, of which the results are as follows. First, our science curriculum focuses on vertical articulation and has relatively neglected the problem of horizontal articulation. To compensate for this problem, curriculum design should introduce aspects of 'nature' and 'environment' and should consider the interests and concerns of students, as countries with high horizontal articulation do. Second, the actual education field has a problem with the a lack of continuity and sequence because of concentration of concept in a specific grade or simply repeating the concept across multiple grades. These results have led to alternative proposals that should arrange basis of concept configuration such as 'Big Idea' and should establish the adoption of 'systems' frequently appearing in the other curricula. Finally, there may be mentioned a lack of research on students' learning progression, which can be a common standard of horizontal and vertical articulation. Research on learning progression has been a trend overseas, but there exists no study to fit Korea's situation, so education fields need to conduct the appropriate research on learning progression as part of the commitment to high-quality curriculum.

Density Effects on the Size of 2-1 Korean Pine and 1-1 Jack Pine Nursery Stock (잣나무 2-1 묘(苗)와 방크스소나무 1-1 묘(苗)의 생장(生長)에 미치는 밀도효과(密度効果))

  • Ma, Sang Kyu
    • Journal of Korean Society of Forest Science
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    • v.32 no.1
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    • pp.1-8
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    • 1976
  • One of the most common needle leaf species used in planting in Korea is korean pine (Pinus koraiensis S. et Z.), and jack pine (Pinus banksiana Lamb.) is one of the test species for suitability. The relation of nursery bed density of 2-1 korean pine and 1-1 jack pine was studied at the Kwang Nung Nursery, Central Branch Station of Forest Research Institute, and about 40km north of Seoul. Nursery bed density of 2-1 korean pine, which ranged from 36 to 324 trees per square meters and of 1-1 jack pine, which ranged from 25 to 169 trees per square meters, had a marked effect on caliper, height, dry weight and percent and amount of plantable stock. The soil physical and chemical properties is silt plus clay, 50.55 percent; organic matter, 2.09 percent; total nitrogen, 0.13 percent; available phosphorus, 253.25 ppm; exchangeable potash, 0.46 m.e/100g; and pH, 5.58. As the density of the nursery seedling stand of 2-1 korean pine increases, the average tree height increases (Fig. 1A), but in 1-1 jack pine density do not affect to increase or decrease the average tree height. As the density of nursery bed increases, the average stem caliper (at 2cm above ground line) and dry weight decrease (Fig. 1B), but the decreasing rate is more seriously in 1-1 jack pine than 2-1 korean pine (Fig.5). As increasing the density of nursery bed, the T/R ratio of trees of the test species increase. Also the dry weight of leaf, stem and root parts are decreasing in proportion to the increase of stand density, but the drop rate of jack pine is more rapid than korean pine (table. 1) The patent facts of difference of growth characteristics between 2-1 korean pine and 1-1 jack pine were studied. These facts should be used to select the scale of stand density at the nursery bed or the plantable site. Korean pine is demanded high density, on the other hand in jack pine low density are more suitable to manage the stand density. Stands of comparatively low density had the greatest percentage of high-quality stock, and the stands of high density had less than the high quality trees of low density. An important criterion of the best density is percent and number of high-quality trees produced per square meter of bed area. Stem caliper and stem height of seedling is used in most public nurseries to sort seedling into plantable grades. The stock grade standard has set at 4.5mm caliper and 16cm height of 2-1 korean pine as the minimum desired stem caliper and height. By the result studies, the plantable stock grade standards of 2-1 korean pine used at stem height 16cm and stem caliper 4.5mm from public nurseries should be reformed to stem height 18cm and stem caliper 4.0mm by the growth characteristics and the tree distribution of stem height and caliper of relation to density. For the 2-1 korean pine, best density should be about 160 to 200 trees per square meter according to soil fertility. For the 1-1 jack pine, the suitable standard of plantable stock should be at stem height 25cm and caliper 6mm (at 2cm above ground line) and best density was about 100 to 120 trees.

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