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Analysis of Vegetational Community Structure and Phytosociological Changes During Eight Years of the Namsan Nature Park in Seoul (남산자연공원(南山自然公園)의 식물군집구조(植物群集構造) 및 8년간(年間)의 식생변화분석(植生變化分析))

  • Lee, Kyong-Jae;Park, In-Hyeop;Oh, Koo-Kyoon
    • Journal of Korean Society of Forest Science
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    • v.76 no.3
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    • pp.206-217
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    • 1987
  • To investigate and analyze the vegetational structure, thirty sites were sampled with clumped sampling method and seventeen belt transects were sampled to analyze the vegetational change during 8 years(from 1978 to 1986)at the Namsan Nature Park in Seoul. Robinia pseudoacacia community of actual vegetation covered 29.39%, Quercus mongolica community 21.25% and Pinus densiflora community 17.58% of the Mt. Namsan forest. The degree of human disturbance of vegetation of 8, 7 and 6 area covered 43.2%, 7.8% and 30.8% respectively. The Quercus mongolica community at the northern slope and R. pseudoacacia community at the edge zone will keep their present structure. And P. densiflora community at the eastern and southern slope shall be succeeded to deciduous trees such as Q. mongolica. As to changes of plant community structure during 8 years, density of understory species, species diversity and Raunkiaer's frequency class A and E showed an increase and vegetation structure was developed to unification.

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Copper and Zinc Uptake Capacity of a Sorghum-Sudangrass Hybrid Selected for in situ Phytoremediation of Soils Polluted by Heavy Metals (식물정화를 위한 중금속 내성 작물의 선발과 수수-수단그라스 교잡종의 구리와 아연 흡수능력)

  • Oh, Soon-Ja;Koh, Seok-Chan
    • Journal of Environmental Science International
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    • v.24 no.11
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    • pp.1501-1511
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    • 2015
  • As essential trace elements, copper and zinc play important roles in many physiological events in plants. In excess, however, these elements can limit plant growth. This study selected a heavy metal-tolerant plant by analyzing seed germination and biomass of alfalfa (Medicago sativa), canola (Brassica campestris subsp. napus var. nippo-oleifera), Chinese corn (Setaria italica), and a sorghum-sudangrass hybrid (Sorghum bicolor ${\times}$ S. sudanense), and determined heavy metal uptake capacity by analyzing biomass, chlorophyll a fluorescence, and heavy metal contents under high external copper or zinc levels. The seed germination rate and biomass of the sorghum-sudangrass hybrid were higher under copper or zinc stress compared to the other three plants. The plant biomass and photosynthetic pigment contents of the sorghum-sudangrass hybrid seedlings were less vulnerable under low levels of heavy metals (${\leq}50ppm$ copper or ${\leq}400ppm$ zinc). The maximum quantum yield of PSII ($F_v/F_m$) and the maximum primary yield of PSII ($F_v/F_o$) decreased with increasing copper or zinc levels. Under high copper levels, the decline in $F_v/F_m$ was caused only by the decline in $F_m$, and was accompanied by an increase in non-photochemical quenching (NPQ). The $F_v/F_m$ declined under high levels of zinc due to both a decrease in the maximum fluorescence ($F_m$) and an increase in the initial fluorescence ($F_o$), and this was accompanied by a marked decrease in photochemical quenching (qP), but not by an increase in NPQ. Accumulations of copper and zinc were found in both aboveand below-ground parts of plants, but were greater in the below-ground parts. The uptake capacity of the sorghum-sudangrass hybrid for copper and zinc reached 4459.1 mg/kg under 400 ppm copper and 9028.5 mg/kg under 1600 ppm zinc. Our results indicate that the sorghum-sudangrass hybrid contributes to the in situ phytoremediation of copper or zinc polluted soils due to its high biomass yield.

Investigation and Analysis of Allergy-related SNPs for Allergy Affected Students in a high school. (과학영재학교 학생들이 알러지 관련 SNP 탐색고 분석)

  • 김경원;이호경;김현근;김수영;안정훈
    • Journal of Life Science
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    • v.14 no.5
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    • pp.847-854
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    • 2004
  • Allergy is a multi-factorial disease influenced by genetic and environmental factors. As the number of allergy-affected people is increasing in developed countries, there is an increasing interest in genetic predisposition to the allergy. A number of genes and chromosomal region have been identified to be linked to allergy including rhinitis, asthma and atopy. In order to understand the genetic background for the allergy-affected people, we investigated genetic predisposition among students enrolled in Busan Science Academy. Among 138 students, about 30% students had some allergy-related disorder including rhinitis, asthma and atopy. We analyzed several single nucleotide polymorphisms (SNPs) within two genes, Inter-leukin-4(IL-4) and Interleukin-4 receptor(IL-4R), which are involved in the induction of allergy reaction with the Th2 immunity. For 96 samples obtained from students, we analyzed 9 SNPs including -590 C/T and -34 C/T in IL-4, and I75V, Q576R, E375A, e406R, 5411L, S761P and S727A in IL-4R. From the analysis, these SNPs showed slight differences among normal and allergy-affected students, but these differences was not enough to predict the predisposition to the allergy. In contrast to previous reports, we could not find SNP(s) related with allergy. These results suggest that genetic tests recently performed in Korea widely have to be reassessed for its validity of genetic predisposition. [Supported by grants from MOST]

Syntaxonomy of the Forest Vegetation and Surrounding Taegu, Korea (대구 인접지역에 대한 삼림식생의 군락분류)

  • Choung, Heung-Lak;Lee, Ho-Joon;Lee, Jae-Seok
    • The Korean Journal of Ecology
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    • v.23 no.5
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    • pp.407-421
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    • 2000
  • The forest vegetation surrounding Taegu area was investigated by the methodology of the ZM school of phytosociology, from September, 1994 to August, 1997. The forest vegetation was classified into 7 communities, 8 subcommunities and 5 afforestations as follows : Quercus mongolica community (Typical subcommunity, Carex siderosticta subcommunity), Quercus variabilis community, Quercus acutissima community, Quercus dentate community, Carpinus cordata-Acer mono community (Typical subcommunity, Quercus serrata subcommunity, Cornus controversa subcommunity), Pinus densiflora community (Typical subcommunity, Sanguisorba officinalis subcommunity, Rhododendron schlippenbachii subcommunity), Lespedeza maximowiczii-Rhododendron mucronulatum community , Robinia pseudo-acacia afforestation, Pinus rigida afforestation, Larix leptolepis afforestation, Pinus thunbergii afforestation, Pinus koraiensis afforestation. The differential species of the Quercus mongolica community were Quercus mongolica, Athyrium yokoscense, Rhododendron schlippenbachii, Fraxinus sieboldiana, Acer pseudo-sieboldianum, Symplocos chinensis for. pilosa, Astilbe chinensis var. davidii, Weigela subsessilis, and Melampyrum roseum, those of the Carpinus cordata-Acer mono community were Acer mono, Carpinus cordata, Carpinus laxiflora, and Staphylea bumalda, those of the Pinus densiflora community were Pinus densiflora, Quercus serrata, Cocculus trilobus, and Juniperus rigida. Constance degree of the Quercus serrate was especially high in shrub and herb layers of the Pinus densiflora community. The results of factor analysis by DCA showed that distribution pattern of the communities were similar to that by Z-M method and that communities were arranged according to soil moisture gradient. Rhododendron schlippenbachii subcommunity of the P. densiflora community and the Typical subcommunity of the Q. mongolica community were distributed in similar environments.

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Determination and Prediction of the Amino Acid Digestibility of Sunflower Seed Meals in Growing Pigs

  • Liu, J.D.;Li, Q.Y.;Zeng, Z.K.;Li, P.;Xu, X.;Wang, H.L.;Zhang, S.;Piao, X.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.86-94
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    • 2015
  • This experiment was conducted to evaluate the chemical composition and amino acid (AA) digestibility of sunflower seed meal (SFSM) and to use this data to develop prediction equations for estimating AA digestibility for growing pigs. Ten SFSM were collected from five provinces in China. Twelve barrows ($38.8{\pm}4.6kg$), fitted with ileal T-cannula were allotted into two $6{\times}6$ Latin square designs. Each of six experimental periods comprised a 5-d adaption period followed by a 2-d collection of ileal digesta. The ten test diets contained 50% SFSM as the sole source of AA. Another nitrogen-free diet was used to measure the basal endogenous losses of crude protein (CP) and AA. Chromic oxide (0.3%) was used as an inert marker in each diet. There was considerable variation (CV>10%) among the ten SFSM in chemical composition (dry matter [DM]). The concentration of CP and ether extract (EE) ranged from 29.33% to 39.09% and 0.88% to 11.33%, respectively. Crude fibre (CF), neutral detergent fibre and acid detergent fibre ranged from 21.46% to 36.42%, 38.15% to 55.40%, and 24.59% to 37.34%, respectively. There was variation among the ten SFSM in apparent ileal digestibility (AID) and standardized ileal digestibility (SID) for lysine and threonine, which ranged from 63.16 to 79.21 and 55.19% to 72.04% for AID and 67.03% to 82.07% and 61.97% to 77.01% for SID, respectively. The variation in CP and methionine ranged from 60.13% to 74.72% and 74.79% to 88.60% for AID and 66.70% to 79.31% and 77.16% to 90.27% for SID, respectively. Methionine was a good indicator to predict AA digestibility. These results indicate that conventional chemical composition of SFSM was variable (CV>10%) among the ten SFSM (DM). The results of AID, SID and prediction equations could be used to evaluate the digestibility of SFSM in growing pigs.

Effects of Donor Cell Passage, Size and Type on Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer

  • Zhang, Y.H.;Song, E.S.;Kim, E.S.;Cong, P.Q.;Lee, S.H.;Lee, J.W.;Yi, Y.J.;Park, Chang-Sik
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.2
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    • pp.194-200
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    • 2009
  • The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductal epithelial cells from 1-2, 3-6 and 7-10 passages were used for the nuclear transfer. In the oocytes with the cumulus donor cells, fusion and cleavage rates of oocytes and cell numbers per blastocyst among the three different passage groups did not show any differences, but the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher than those from 7-10 passage group. The rates of fusion, cleavage and blastocyst formation, and the cell numbers per blastocyst were higher in the embryos with the sizes of <20 and 20 ${\mu}m$ cumulus donor cells compared to the >20 ${\mu}m$ cumulus donor cell. In the oocytes with the fetal fibroblast donor cells, the rate of blastocyst formation from the 3-6 passage group was higher than from 1-2 and 7-10 passage groups. The embryos with the size of 20 $\mu{m}$ fetal fibroblast donor cell showed higher rate of blastocyst formation compared to those with <20 and >20 ${\mu}m$ donor cells. In the oocytes with the oviductal epithelial cells, the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher compared to those from 7-10 passage group. The embryos with the sizes of <20 and 20 ${\mu}m$ oviductal epithelial donor cells had a higher rate of blastocyst formation compared to those with >20 ${\mu}m$ donor cell. Fusion and cleavage rates of oocytes, and cell numbers per blastocyst among the three different donor cell types from the 3-6 passage did not show any differences. However, the rate of blastocyst formation of somatic cell nuclear transfer (SCNT) embryos with the fetal fibroblast donor cell was higher than that of blastocyst formation of SCNT embryos with the cumulus and oviductal epithelial donor cells.

The Effect of NiO Addition to the PNN-PZT Piezoelectric Ceramics on Piezoelectric Properties (Pb(Ni1/3Nb2/3)O3-PZT 세라믹스 고용체에서 과잉 NiO첨가에 따른 압전특성 변화)

  • Choi Y. G.;Son Y. J.;Kweon J. C.;Cho K. W.;Yoon M. S.;Kim I. H.;Kim Y. M.;Ur S. C.
    • Korean Journal of Materials Research
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    • v.15 no.6
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    • pp.413-418
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    • 2005
  • Perovskite $Pb(Ni_{1/3}Nb_{2/3})O_3-Pb(Zr,Ti)O_3[PNN-PZT]$ ceramics were synthesized by conventional ceramic processing technique. In order to modify piezoelectric properties for sensor application in this system, NiO addition was considered to provide $Ni^{+2}$ as an acceptor, which was known to occupy with B site in the structure. The effect of NiO addition up to $8\;mol\%$ on the following piezoelectric properties as well as sintering properties was investigated. When NiO added more than $1\;mol\%$, average grain size was decreased and second phase was found to form. Moreover, the second phase caused decrease in relative dielectric constant $(\varepsilon_{33}T/\varepsilon0)$, electro-mechanical coupling factor $(k_p)$, and piezoelectric charge constant $(d_{33})$, while increasing mechanical quality factor $(Q_m)$. When $1\;mol\%$ NiO was added, density, dielectric properties and piezoelectric properties were abruptly increased.

Salinity Tolerance of Blackgram and Mungbean: II. Mineral Ions Accumulation in Different Plant Parts

  • Karim, M.A.;Raptan, P.K.;Hamid, A.;Khaliq, Q.A.;Solaiman, A.R.M.;Ahmed, J.U.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.5
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    • pp.387-394
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    • 2001
  • Blackgram (Vigna mungo) is more salt tolerant than mungbean (Vigna radiata). This study was initiated to know whether the accumulation pattern of mineral ions in different plant parts plays a significant role in the differences in salt tolerance between the two Vigna species. Different mineral ions, viz. N, Cl, Na, K, Mg and Ca in different organs of two varieties of each of blackgram- Barimash-l (susceptible one) and Barimash-2 (tolerant one), and mungbean-Barimung-3 (tolerant one) and Barimung-4(susceptible one), were analyzed after growing with 0, 50, 75 and 100 mM NaCl solutions. The two crops showed a decreased but similar pattern of total N accumulation under saline conditions. The tolerant variety of both the crops showed a less reduction in total N than the susceptible one. Leaves showed the maximum while stem the minimum N, irrespective of levels of salinity. C $l^{[-10]}$ and N $a^{+}$ accumulation increased with the increasing salinity levels. Interestingly, similar to a halophyte, the salt tolerant blackgram exhibited conspicuously higher amount of N $a^{+}$ in the shoot than the salt-susceptible mungbean. However, the tolerant varieties showed less amount of N $a^{+}$ than the susceptible one, especially in blackgram. Seeds of both Vigna spp. accumulated the minimum amount of N $a^{+}$ than other plant parts. $K^{+}$ accumulation decreased by salinity in most of the plant parts, except seeds. Blackgram showed larger reduction in K than mungbean. The $Mg^{++}$ increased in leaves, petioles and stem by salinity while decreased in the roots, podshells and seeds in both the crops. Salinity increased $Ca^{++}$ accumulation in all plant-parts except roots of both Vigna spp. Apparently, the leaves of mungbean accumulated higher concentration of $Ca^{++}$ than blackgram. Varietal differences in the accumulation pattern of $K^{+}$, $Mg^{++}$ and $Ca^{++}$ were not clear. It was concluded that blackgram, presumably, possesses a similar salt tolerance mechanism to halophyte, and the pattern of accumulation of mineral ions in blackgram and mungbean was not fully ascribed to the differences in salinity tolerance between the two Vigna species.gna species.ies.s.ies.

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Improving Oxygenation in the Murine Tumors by a perfluorochemical Emulsion (Fluosl-DA $20\%$ (Carbogen 흡입하에서 Fluosol-DA 20%의 투여가 이식동물 종양의 산소분압에 미치는 영향)

  • Lee Intae;Kim Gwi E.;Song Chang W.
    • Radiation Oncology Journal
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    • v.8 no.1
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    • pp.1-6
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    • 1990
  • In the present study, a perfluorchemical emulsion (Fluosol-DA $20\%$) did not alter $D_o\;and\;D_q$ values on cell survival curve, indicating that the lack of a direct effect of Fluosol-DA $20\%$ on cellular radiosensitivity in vitro. The effect of Fluosol-DA $20\%$ injection in combination with carbogen breathing was determined on the hypoxic cell fraction in SCK tumors. The hypoxic cell fraction in control SCK tumors was 0.39. This value decreased to 0.05 when the mice were i.v. injected with 12 ml/kg of Fluosol-DA $20\%$ in a carbogen atomosphere. The measured mean and median $PO_2$ values with a microelectrode in the control tumors was 9 mmHg and 4 mmHg, respectively. The treatment of the SCK tumors in the host mice with injected Fluosol-DA $20\%$ in combination with carbogen breathing increased the mean and median $PO_2$ values to 67 mmHg and 62 mmHg, respectively. Using carbogen breathing alone caused a moderate increase of tumor $PO_2$. But Fluosol-DA $20\%$ injection alone caused little change $PO_2$ in the tumor. It was concluded that the combination of Fluosol-DA injection and carbogen breathing is an effective means to improve oxygenation of tumors.

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Overexpression of an oligopeptide transporter gene enhances heat tolerance in transgenic rice (Oligopeptide transporter 관여 유전자 도입 형질전환벼의 고온스트레스 내성 증진)

  • Jeong, Eun-Ju;Song, Jae-Young;Yu, Dal-A;Kim, Me-Sun;Jung, Yu-Jin;Kang, Kwon Kyoo;Park, Soo-Chul;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.44 no.3
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    • pp.296-302
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    • 2017
  • Rice (Oryza sativa) cultivars show an impairment of growth and development in response to abiotic stresses such as drought, salinity, heat and cold at the early seedling stage. The tolerance to heat stress in plants has been genetically modulated by the overexpression of heat shock transcription factor genes or proteins. In addition to a high temperature-tolerance that has also been altered by elevating levels of osmolytes, increasing levels of cell detoxification enzymes and through altering membrane fluidity. To examine the heat tolerance in transgenic rice plants, three OsOPT10 overexpressing lines were characterized through a physiological analysis, which examined factors such as the electrolyte leakage (EL), soluble sugar and proline contents. We further functionally characterized the OsOPT10 gene and found that heat induced the expression of OsOPT10 and P5CS gene related proline biosynthesis. It has been suggested that the expression of OsOPT10 led to elevated heat tolerance in transgenic lines.