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http://dx.doi.org/10.5010/JPB.2017.44.3.296

Overexpression of an oligopeptide transporter gene enhances heat tolerance in transgenic rice  

Jeong, Eun-Ju (Department of Crop Science, Chungbuk National University)
Song, Jae-Young (Department of Crop Science, Chungbuk National University)
Yu, Dal-A (Department of Crop Science, Chungbuk National University)
Kim, Me-Sun (Department of Crop Science, Chungbuk National University)
Jung, Yu-Jin (Department of Horticultural Life Science, Hankyong National University)
Kang, Kwon Kyoo (Department of Horticultural Life Science, Hankyong National University)
Park, Soo-Chul (The National Center for GM Crops, Rural Development Administration (RDA))
Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 296-302 More about this Journal
Abstract
Rice (Oryza sativa) cultivars show an impairment of growth and development in response to abiotic stresses such as drought, salinity, heat and cold at the early seedling stage. The tolerance to heat stress in plants has been genetically modulated by the overexpression of heat shock transcription factor genes or proteins. In addition to a high temperature-tolerance that has also been altered by elevating levels of osmolytes, increasing levels of cell detoxification enzymes and through altering membrane fluidity. To examine the heat tolerance in transgenic rice plants, three OsOPT10 overexpressing lines were characterized through a physiological analysis, which examined factors such as the electrolyte leakage (EL), soluble sugar and proline contents. We further functionally characterized the OsOPT10 gene and found that heat induced the expression of OsOPT10 and P5CS gene related proline biosynthesis. It has been suggested that the expression of OsOPT10 led to elevated heat tolerance in transgenic lines.
Keywords
Oligopeptide transporter; Heat stress; Tolerance; Transgenic rice;
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