• Mycobiology
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• v.38 no.4
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• pp.331-335
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• 2010

In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using the partial ftx gene (410 bp) along with the hygromycin B phosphotransferase gene (hygB) downstream of the glyceraldehydes-3-phosphate dehydrogenase (gpd) promoter. The plasmid was transformed into protoplasts of monokaryotic strain 4019-20 of F. velutipes by electroporation. High transformation efficiency was obtained with an electric-pulse of 1.25 kV/cm by using 177 transformants/${\mu}g$ of DNA in $1{\times}10^7$ protoplasts. PCR and Southern blot hybridization indicated that a single copy of the plasmid DNA was inserted at different locations in the F. velutipes genome by non-homologous recombination. Therefore, this transformation system could be used as a useful tool for gene function analysis of F. velutipes.

• Journal of Power Electronics
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• v.13 no.5
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• pp.806-813
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• 2013

This paper aims to compare the performance of three phase induction motor drives using Five Leg Inverter (FLI) and Three Leg Inverter (TLI) configurations. An Indirect Field Oriented Control (IFOC) method using a TLI is well established and incorporated for high performance speed drives in various industries. The FLI dual motor drive system on the other hand shows good workability in the independent control of two induction motor drives simultaneously. In this experiment, the IFOC method is utilized for both drive systems, and Space Vector Pulse Width Modulation (SVPWM) is used to generate pulses for both inverters. For the FLI, the Double Zero Sequence (DZS) Injection technique is used to generate the modulation signal. The complete experiment setup is done by using a DSpace 1103 controller board. The individual motor performances are analyzed using similar schemes, equipment setups and controller parameter values. The results show similar speed performance response capability between the single motor operation using a TLI system and the two motor operation using a FLI system based on the variable speed range either in forward or reverse operation. They also show similar load rejection abilities. However, the single motor with a TLI has a better power quality aspect such as ripple current and total harmonics distortion (THD).

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.51 no.12
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• pp.584-590
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• 2002

Crowbar system is usually applied to a pulsed power system in which a capacitor bank is discharged into a load. This provides a free wheeling path for the load current and prevents the capacitor from recharging due to a reverse voltage. Usually diodes have been used as a crowbar switch, but it is not a practical system because the cost of the diodes goes up enormously with increasing the system voltage and current. This paper presents a novel protection scheme of a charging and discharging system of a 300 kJ capacitor bank using a low-cost crowbar system which consists of a crowbar switch and resistors. Triggered vacuum switch(TVS) was used for a crowbar switch, and Rogowski coil was used to determine a trigger time of TVS. When this crowbar system is applied to our pulsed power system which consists of capacitor bank($123muF$), inductor() for forming a pulse, load resistor$(100 m\Omega)$, and a closing switch, instantaneous reversal voltage of capacitor bank could be limited less than 1.8 ㎸ until capacitor bank was charred to 17 ㎸.

• Journal of the Microelectronics and Packaging Society
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• v.14 no.3
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• pp.37-42
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• 2007

Copper deposition studies have been actively studied since interests on 3D SiP were increased. The defects inside via can be easily formed due to the current density differences on entrance, bottom and wall of via. So far many different additives and current types were discussed and optimized to obtain void-free copper via filling. In this research acid cupric sulfate plating bath containing additives such as PEG, SPS, JGB, PEI and wave current applied electroplating were examined. The size and shape of grain were influenced by the types of organic additives. The cross section of specimen were analyzed by FESEM. When PEI was added, the denser copper deposits were obtained. Electroplaing time was reduced when 2 step via filling was employed.

• Journal of Power Electronics
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• v.12 no.3
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• pp.377-386
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• 2012

An interleaved bridgeless buck-boost AC/DC converter is presented in this paper to achieve the characteristics of low conduction loss, a high power factor and low harmonic and ripple currents. There are only two power semiconductors in the line current path instead of the three power semiconductors in a conventional boost AC/DC converter. A buck-boost converter operated in the boundary conduction mode (BCM) is adopted to control the active switches to achieve the following characteristics: no diode reverse recovery problem, zero current switching (ZCS) turn-off of the rectifier diodes, ZCS turn-on of the power switches, and a low DC bus voltage to reduce the voltage stress of the MOSFETs in the second DC/DC converter. Interleaved pulse-width modulation (PWM) is used to control the switches such that the input and output ripple currents are reduced such that the output capacitance can be reduced. The voltage doubler topology is adopted to double the output voltage in order to extend the useable energy of the capacitor when the line voltage is off. The circuit configuration, principle operation, system analysis, and a design example are discussed and presented in detail. Finally, experiments on a 500W prototype are provided to demonstrate the performance of the proposed converter.

• Korean Journal of Optics and Photonics
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• v.15 no.5
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• pp.449-454
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• 2004

The optical limiter is an optical component which reduces laser beam intensity for the protection of eyes and light sensors. Carbon nanotube is now known as a highly efficient optical limiting material. Optical limiting effect of the multi-walled carbon nanotube suspensions, in several kinds of solvents such as distilled water, chloroform, ethanol and ethylene glycol, were measured in the range from room temperature to near to the boiling points of the solvents. The pulsed Nd:YAG laser whose wavelength is 1064 nm and pulse duration is 6 ns was used as a light source. The experimental result shows that the limiting efficiency was reduced as the temperature increased, and the suspension which has lower boiling point, viscosity and surface tension has highest efficiency.

• Journal of Power Electronics
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• v.16 no.6
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• pp.2099-2108
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• 2016

The cascaded multilevel converter is widely adopted to medium/high voltage and high power electronic applications due to the small harmonic components of the output voltage and the facilitation of modularity. In this paper, the operation principle of a T-type H-bridge topology is investigated in detail, and a carrier phase shifted pulse width modulation (CPS-PWM) based control method is proposed for this topology. Taking a virtual five-level waveform achieved by a unipolar double frequency CPS-PWM as the output object, PWM signals of the T-type H-bridge can be obtained by reverse derivation according to its switching modes. In addition, a control method for the T-type H-bridge based cascaded multilevel converter is introduced. Then a single-phase T-type H-bridge cascaded multilevel static var generator (SVG) prototype is built, and a repetitive controller based compound current control strategy is designed with the DC-link voltage balancing control scheme analyzed. Finally, simulation and experimental results validate the correctness and feasibility of the proposed modulation method and control strategy for T-type H-bridge based cascaded multilevel converters.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.8 no.6
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• pp.645-655
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• 1997

In this paper, the performance of the noncoherent code tracking loop designed at baseband for CDMA applications is analyzed in detail and is confirmed by computer simulations. Analytical closed-form formula for jitter variance is derived for AWGN channel environments as a function of pulse shaping filter, timing offset, signal-to-interference ratio, and loop filter coefficients. The design issue of the loop filter is also addressed with emphasis on the second-order tracking loop. Finally, the performance of the designed tracking loop is examined by computer simulations for both AWGN and Rayleigh fading channels, when applied to the reverse link of the coherent CDMA system for IMT-2000 designed by ETRI.

• Journal of Power Electronics
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• v.17 no.6
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• pp.1391-1401
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• 2017

In this paper, a secondary resonance half-bridge dc-dc converter with an inductive output filter is presented. The primary side of such a converter utilizes asymmetric pulse width modulation (APWM) to achieve zero-voltage switching (ZVS) of the switches, and clamps the voltage of the switch to the input voltage. In addition, zero current switching (ZCS) of the output diode is achieved by a half-wave rectifier circuit with a filter inductor and a resonant branch in the secondary side of the proposed converter. Thus, the switching losses and diode reverse-recovery losses are eliminated, and the performance of the converter can be improved. Furthermore, an inductive output filter exists in the converter reduce the output current ripple. The operational principle, performance analysis and design equation of this converter are given in this paper. The analysis results show that the output diode voltage stress is independent of the duty cycle, and that the voltage gain is almost linear, similar to that of the isolation Buck-type converter. Finally, a 200V~380V input, 24V/2A output experimental prototype is built to verify the theoretical analysis.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.7
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• pp.1777-1783
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• 2014

In this paper, we designed and fabricated a high-speed semiconductor sensor for use in power control devices and analyzed the characteristics with pulsed radiation tests. At first, radiation sensitive circular Si PIN diodes with various diameters(0.1 mm ~5.0 mm) were designed and fabricated using Si epitaxial wafer, which has a $42{\mu}m$ thick intrinsic layer. The reverse leakage current of the diode with a radius of 2 mm at a reverse bias of 30 V was about 20.4 nA. To investigate the characteristic responses of the developed diodes, the pulsed gamma-radiation tests were performed with the intensity of 4.88E8 rad(Si)/sec. From the test results showing that the output currents and the rising speeds have a linear relationship with the area of the sensors, we decided that the optimal condition took place at a 2 mm diameter. Next, for the selected 2 mm diodes, dose rate tests with a range of 2.47E8 rad(Si)/sec to 6.21E8 rad(Si)/sec were performed. From the results, which showed linear characteristics with the radiation intensity, a large amount of photocurrent over 60mA, and a high speed response under 350ns without saturation, we can conclude that the our developed PIN diode can be a good candidate for the sensor of power control devices.