• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.103.1-103.1
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• 2003

This study was carried out to investigate sulfur dioxide in herb drugs.(Puerariae Radix, Lycii Fructus, Platycodi Radix, Dioscoreae Rhizoma, Mori Radicis Cortex, Nelumbo Seed, Paeoniae Radix, Remotiflori Radix, Astragali Radix, Polygonatum Rhizome) Experimental subjects included 105 cases of 10 species of herb drugs collected in Kyung-dong market from December in 2001 to February in 2002. Sulfur dioxide was determined by Optimized Monier-Wiliams method. In 65 cases(61.9%) of the 105 cases, we detected $SO_2$ over 10mg/kg. (omitted)

• 한국식품영양과학회지
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• 제31권5호
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• pp.931-938
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• 2002

유용세균인 B. infantis은 선택되어진 천연산물의 한 종류 단독으로서도 촉진되었으며, 또한 두 종류, 세 종류, 네 종류의 배합에 의하여 특징적으로 성장이 촉진되었으며, 이것은 항산화력이 강한 천연산물들을 free radical의 제거와 동시에 B. infantis의 성장촉진물질로 사용할 수 있고 이를 이용하여 기능성 항산화 제제의 제조가 가능함을 의미하며 이들 천연산물의 배합 중에서 한 종류의 천연산물로서는 진피, 두충 ,산수유 그리고 황기 등이 좋은 효과를 보였고, 두 종류 천연산물의 배합에서는 음양곽과 녹차, 음양곽과 산사자, 음양곽과 두충 등의 조합이, 세 종류의 천연산물 배합에서는 녹차와 음양곽 및 백굴채, 녹차와 음양곽 및 진피, 녹차와 백굴채 및 황기, 녹차와 진피 및 갈근 등의 조합이, 4종류 천연산물 배합에서는 녹차, 황기 및 음양곽 그리고 갈근의 조합, 녹차, 황기 및 진피 그리고 갈근의 조합, 녹차, 백굴채 및 황기 그리고 음양곽 등의 조합에서 B. infantis가 잘 성장함을 보였고, 녹차, 백굴채 및 황기 그리고 음양곽의 조합이 대조군보다 2.1배 이상의 성장률을 보였다. 4종류의 조합의 항산화력을 용존산소 분석법에 의하여 분석한 결과 녹차, 백굴채 및 황기 그리고 음양곽의 조합이 632.49로써 가장 높았고, 다음으로 녹차, 음양곽 및 진피 그리고 갈근의 조합(623.64),녹차, 백굴채 및 진피 그리고 갈근의 조합(601.81), 녹차, 백굴채 및 황기 그리고 갈근의 조합(560.49)순서로 나타났으며, 항산화력이 강한 조합에서 성장이 촉진되는 것으로나타났다. B. infantis의 성장을 촉진하는 4종류의 조합에 대한 hydroxyl radical의 소거 활성은 50,100,200 및 300 배로 희석하여 검토하였을 때 녹차, 백굴채 및 진피 그리고 갈근의 조합을 100배로 희석한 소거윤이 84.36%로 가장 높은 소거율을 보였으며 ,4 종류의 조합모두 놀은 희석율에도 80% 이상의 높은 소거율을 보여 높은 희석률을 필요로 하는 음료 제제로서의 탁월함을 보였다. 또한, 4종류의 각 조합에 따른 방향족의 함량 및 총 Phenol 함량은 녹차 중의 증제차나 볶음차의 비 발효 차보다 28～30배 이상 높은 것으로 나타났으며, 반발효차인 오룡차보다는, 32～33배, 발효차인 홍차보다는 55～58배정도 높은 것으로 나타났다 이것은 항산화력이 강한 천연산물들을 이용하여 free radical의 제거와 동시에 B. infantis의 성장 촉진물질로 사용할 수 있으며 생체의 기능을 향상시킬 수 있는 기능성 제제로의 응용 가능성을 보였다.

• Natural Product Sciences
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• 제6권4호
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• pp.165-169
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• 2000

In vitro cytotoxic activities of cisplatin combined with Chungpesagan-tang or puerarin, which were treated with or without human intestinal bacteria, were measured. When cisplatin was combined with Chungpesagan-tang and its ingredient treated without intestinal bacteria, they did not affect the in vitro cytotoxicity of cisplatin against tumor cell lines. However, when cisplatin was combined with intestinal bacteria-treated Chungpesagan-tang and its ingredients, the cytotoxicities against SNU C4, L1210, A549 and P388 tumor cell lines were synergistically increased. Puerarin, which was isolated from Puerariae Radix, did not show in vitro cytotoxicity. However, its metabolite, daidzein, showed potent cytotoxicity against tumor cell lines and was synergistic by the combined usage of cisplatin. These results suggest that natural glycosides are not only prodrugs which can be transformed to active compounds by intestinal microflora, but the combined usage of cisplatin with natural components, such as daidzein, and herbal medicinal polyprescriptions, such as Chungpesagan-tang, may be a new method for prevention and minimization of the toxicity of cisplatin.

• 대한한방소아과학회지
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• 제23권3호
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• pp.217-231
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• 2009

Objectives To evaluate that Okwada affected which factors for treatment of lung fibrosis. Methods Bleomycin induced lung fibrosis model made in mice. After Okwada lyophilized, power sample obtained and melt in distilled water. Okwada solution administered mice through oral route on 21 days after bleomycin instillation and this procedure performed once a day for 7 days. We divided by three groups; normal (control), bleomycin induced lung fibrosis without treatment (experimental), bleomycin induced lung fibrosis with treatment (treatment). On six weeks after bleomycin instillation, mice sacrificed and removed lung. We performed Western blot analysis for TGF-beta, phosphodiesterase 5A, interleukin (4,5,13) and compared therapeutic effects of Okwada. Results On western blot analysis, all normal and experimental mice detected TGF-beta, phosphodiesterase 5A, interleukin 4,5,13. The amount of band of TGF-beta, phosphodiesterase 5A, interleukin 5 in experimental and treatment group was similar. However, interleukin 4,13 of treatment group decreased compared with experimental group. Conclusions Okwada would be effected the lung fibrosis through suppression of interleukin 4,13.

• 대한한방내과학회지
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• 제24권1호
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• pp.84-93
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• 2003

Objectives : The aim of this study was to investigate effects of the Sungjuchunggan-tang(Xingjiuqinggan-tang), which has been used for alcoholic diseases in Oriental medicine, on alcoholic metabolism and alcoholic liver damage. Methods : We evaluated the activities of alcohol dehydrogenase(ADH)and aldehyde dehydrogenase(ALDH), and measured the levels of AST, ALT, glucose, triglyceride, HDL-cholesterol, LDL-cholesterol and phospholipid in serum of guinea pigs. Results and Conclusions : In this experiment, Sungjuchunggan-tang, Pharbitidis Semen and Puerariae Radix significantly suppressed the activity of ADH which is a precursor enzyme of acetaldehyde. Sungjuchunggan-tang and Pharbitidis Semen significantly suppressed the activity of ALDH, which is a catabolic enzyme, and increased its level. Due to alcohol consumption, level of ALT and AST were significantly reduced. These experimental results suggest that Sungjuchunggan-tang and Pharbitidis Semen inhibit the formation of acetaldehyde in the metabolism of alcohol, and affect the recovery of weakened liver functions caused by alcohol.

• 동의생리병리학회지
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• 제33권1호
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• pp.63-67
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• 2019

Interaction between epidermis and dermis plays an important role in wound healing and hair follicle formation. This study focused on investigating the potency of ethanol extract of Puerariae Radix (EPR) as cosmetic ingredient using human dermal fibroblasts (hDFn). Our results revealed that EPR suppressed collagenase activity dose-dependently. EPR inhibited activity of $5{\alpha}$-reductase I and II at the final concentration of $25{\mu}g/ml$ in hDFn cells. Also, EPR promoted the proliferation and the ERK activation of cells. ERK phosphorylation by EPR was blocked by specific inhibitor of ERK, PD98059. EPR-induced cell proliferation was blocked by PD98059. This means that EPR could promote the proliferation of hDFn cells via the activation ERK. Collectively, these results suggest that EPR may be used as a new cosmetic ingredient.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.222.1-222.1
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• 2003

This study was carried out to investigate the quality control of domestic and imported Puerariae Radix. It was analyzed by HPLC using ${\mu}$-Bondapak C$\sub$18/ column with 35% MeOH containing 1% CH$_3$3CO$_2$H system as the mobile phase at UV 254nm. Good linearity showed over the range of 10 to 200$\mu\textrm{g}$/$m\ell$(r$^2$=1) for Puerarin, and 0.5 to 10$\mu\textrm{g}$/mg(r$^2$=0.9999) for Daidzein. The average contents of Puerarin and Daidzein were 5.5${\pm}$1.2%(Domestic), 5.3${\pm}$0.7%(Imported), and 0.05${\pm}$0.02%(Domestic), 0.08${\pm}$0.02%(Imported).The average recovery rates of Puerarin and Daidzein were 101.8${\pm}$1.9% and 97.2${\pm}$0.7%, respectively.

• Journal of Pharmaceutical Investigation
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• 제16권1호
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• pp.24-30
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• 1986

Ssangwhatang which has been known to have antipyretic, analgesic and antifatigue effects are composed of Paeoniae Radix, Angelicae gigantis Radix, Astragali Radix, Rehmaniae Radix, Cnidii Rhizoma, Cassiae Cortex, Glycyrrhizae Radix and Puerariae Radix. Effects of some formulations of Ssangwhatang were studied with respect to the analgesic, antipyretic, hypothermic, anticonvulsive and antiinflammatory actions in experimental animals. The results obtained were as follows: 1) Prescription A, B, C and D of Ssangwhatang were showed significant analgesic effect by the acetic acid stimulating method in mice. 2) All Ssangwhatang-prescriptions used in this studies had hypothermic effect in normal mice. 3) Significant antipyretic effect in febrile rabbits were recognized in prescription A and C of Ssangwhatang, while not in prescription B and D of Ssangwhatang. 4) Prescription A, B, C and D of Ssangwhatang showed marked antiinflammatory effect against the edema induced by carrageenin in rats. 5) Prescriptions of Ssangwhatang did not show significant anticonvulsive effect against the convulsion induced by picrotoxin and strychnine in mice. From the above results, analgesic, hypothermic, antipyretic and antiinflammatory effects were recognized in Ssangwhatang. Ssangwhatang A- and C- prescriptions added with Pueraiae Radix showed better effects than prescription B and C of Ssangwhatang.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.841-844
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• 2001

Free radicals cause many diseases and aging. In our body coexist useful and harmful bacteria. Some useful intestinal bacteria such as L. plantarum, B. adolescentis, B .bifidum, B. infantis, C. butyricum were presented positive growth in culture medium bearing natural products. L. plantarum was more effectively grown by mixed natural products of CORNI FRUCTUS+THEAE FOLIUM+COPTIDIS RHIZOMA+PAEONIA JAPONICA, B. adolescentis was BEIAMCANDA CHINENSIS+ANGELICAE GIGANTIS RADIX+EPIMEDII HERBA+PAEONIA JAPONICA, B. bifidum was NELUMBO NUCLFERA GAERTNER+THEAE FOLIUM+BEIAMCANDA CHINENSIS+CORNI FRUCTUS, B. infantis was THEAE FOLIUM+CHELIDONIUM MAJUS L.+ASTRAGALI RADIX+EPIMEDII HERBA, C. butyricum was EPIMEDII HERBA+PUERARIAE RADIX+NELUMBO NUCLFERA GAERTNER+PAEONIA JAPONICA,etc. These natural products will support an important role in bio-industry.

• 약학회지
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• 제43권4호
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• pp.481-486
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• 1999

Excessive or long term ingestion of alcohol may cause hepatitis, cirrhosis, hepatic tumor and so on. Aldehyde and active form of free oxygen that are metabolites of alcohol in liver are the cause of liver cell damage. The main system of alcohol metabolism is composed of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH) and cytochrome P450. In connection with in vivo alcohol metabolism, more than one hundred natural products were screened for inhibition or activation of alcohol dehydrogenase. As a results, we found significant inhibition ($IC_50$) of ADH by methanolic extracts of Puerariae Radix ($61.2{\;}\mu\textrm{g}/ml$), Glycyrrhizae Radix ($105.0{\;}\mu\textrm{g}/ml$), Cinnamomi Ramulus ($7.0{\;}\mu\textrm{g}/ml$), Rhei Rhizoma ($36.7{\;}\mu\textrm{g}/ml$), Mori Cortex Radicis ($106.2{\;}\mu\textrm{g}/ml$), Chrysanthemi Flos ($112.2{\;}\mu\textrm{g}/ml$), Erycibes Caulis ($36.7{\;}\mu\textrm{g}/ml$), and Scutellariae Radix ($122.5{\;}\mu\textrm{g}/ml$)