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http://dx.doi.org/10.15188/kjopp.2019.02.33.1.63

Cosmetic Potency of Puerariae Radix in Dermal Fibroblasts  

Lee, Jae Yun (Department of Herbal Resources, Graduate School of Oriental Medicine, Wonkwang University)
Park, Seo A (Department of Beauty Design Graduate School, Wonkwang University)
Woo, Won Hong (Department of Anatomy, School of Oriental Medicine, Wonkwang University)
Mun, Yeun Ja (Department of Herbal Resources, Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.1, 2019 , pp. 63-67 More about this Journal
Abstract
Interaction between epidermis and dermis plays an important role in wound healing and hair follicle formation. This study focused on investigating the potency of ethanol extract of Puerariae Radix (EPR) as cosmetic ingredient using human dermal fibroblasts (hDFn). Our results revealed that EPR suppressed collagenase activity dose-dependently. EPR inhibited activity of $5{\alpha}$-reductase I and II at the final concentration of $25{\mu}g/ml$ in hDFn cells. Also, EPR promoted the proliferation and the ERK activation of cells. ERK phosphorylation by EPR was blocked by specific inhibitor of ERK, PD98059. EPR-induced cell proliferation was blocked by PD98059. This means that EPR could promote the proliferation of hDFn cells via the activation ERK. Collectively, these results suggest that EPR may be used as a new cosmetic ingredient.
Keywords
Pureraria Lobata; Collagenase; $5{\alpha}$-reductase; ERK; Dermal fibroblast;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Hogan BL. Morphogenesis. Cell. 1999;96:225-33.   DOI
2 Hogan BL, Kolodziej PA. Organogenesis: Molecular Mechanisms of Tubulogenesis. Nat Rev Genet. 2002;3:513-23.   DOI
3 Tracy LE, Minasian RA, Caterson EJ. Extracellular matrix and dermal fibroblast function in the healing wound. Adv Wound Care (New Rochelle). 2016;5:119-36.   DOI
4 Sorrell JM, Caplan AI. Fibroblast heterogeneity: more than skin deep. J Cell Sci. 2004;117:667-75.   DOI
5 Driskell RR, Lichtenberger BM, Hoste E et al. Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature. 2013;504:277-81.   DOI
6 Imokawa, G. Mechanism of UVB-induced wrinkling of the skin: Paracrine cytokine linkage between keratinocytes and fibroblasts leading to the stimulation of elastase. J Investig Dermatol Symp Proc. 2009;14:36-43.   DOI
7 Imokawa, G. Ishida, K. Biological mechanisms underlying the ultraviolet radiation-induced formation of skin wrinkling and sagging I: Reduced skin elasticity, highly associated with enhanced dermal elastase activity, triggers wrinkling and sagging. Int J Mol Sci. 2015;16:7753-75.   DOI
8 Bossi O. Gartsbein M. Leitges M. Kuroki T. Grossman S. Tennenbaum T. UV irradiation increases ROS production via PKCdelta signaling in primary murine fibroblasts. J Cell Biochem. 2008:105:194-207.   DOI
9 Uemura M, Tamura K, Chung S, Honma S, Okuyama A, Nakamura Y, Nakagawa H. Novel 5alpha-steroid reductase (SRD5A3, type-3) is overexpressed in hormone-refractory prostate cancer. Cancer Sci. 2008;99:81-6.   DOI
10 Chen W, Orfanos C. The $5{\alpha}$-reductase system and its inhibitors. Dermatology. 1996;193:177-84.   DOI
11 Bayne E, Flanagan J, Einstein M, Ayala J, Chang B, Azzolina B, Whiting D, Mumford R, Thiboutot D, Singer I. Immunohistochemical localization of types 1 and 2 5-reductase in human scalp. Br J Dermatol. 1999.141:481-91.   DOI
12 The Pharmacopoeia Commission of PRC. Pharmacopoeia of the People's Republic of China Version 2000;1 Beijing: Chemical Industry Press; 2000
13 Luo Q, Hao J, Yang Y, Yi H. Herbalogical textual research on "Gegen." Zhongguo Zhongyao Zazhi. 2007;32:1141-4.
14 Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Shin MK, Lee YJ, Lee YC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY. Boncho-Hak. Young-Lim Press, Seoul. 2007;184-5.
15 Molavi O, Narimani F, Asiaee F, Sharifi S, Tarhriz V, Shayanfar A, Hejazi M, Lai R. Silibinin sensitizes chemo-resistant breast cancer cells to chemotherapy. Pharm Biol. 2017;55:729-39.   DOI
16 Yun YG. Oriental prescription & Explanation. Euiseongdang Publishing, Seoul. 2004. pp 241-2.
17 Park SA, Ko KS, In MH, Mun YJ, Woo WH. Effect of Puerariae Radix Ethanol Extract on the Proliferation of Human Dermal Papilla Cells. Journal of Physiology & Pathology in Korean Medicine. 2017;31(3):167-72.   DOI
18 Adlercreutz H. Phyto-oestrogens and cancer. The Lancet Oncology. 2002;3:364-73.   DOI
19 Whelchel A, Evans J, Posada J. Inhibition of ERK activation attenuates endothelin-stimulated airway smooth muscle cell proliferation. Am J Respir Cell Mol Biol. 1997;16:589-96.   DOI
20 Mella JM, Perret MC, Manzotti M, Catalano HN, Guyatt G. Efficacy and safety of finasteride therapy for androgenetic alopecia: a systematic review. Arch Dermatol. 2010;146:1141-50.   DOI
21 Lee S, Lee YB, Choe SJ, Lee WS. Adverse Sexual Effects of Treatment with Finasteride or Dutasteride for Male Androgenetic Alopecia: A Systematic Review and Meta-analysis. Acta Derm Venereol doi:10.2340/00015555-3035. Epub ahead of print. 2019.   DOI
22 Azzouni F, Godoy A, Li Y, Mohler J. The 5 alpha-reductase I isozyme family: A review of basic biology and their role in human diseases. Adv Urol doi:10.1155/2012/530121. 2011.   DOI
23 Jain R, Monthakantirat O, Tengamnuay P, De-Eknamkul W. Avicequinone C isolated from Avicennia marina exhibits $5{\alpha}$-reductase-Type I inhibitory activity using an androgenic alopecia relevant cell-based assay system. Molecules. 2014;19:6809-21.   DOI
24 c The Pharmacopoeia Commission of PRC. Pharmacopoeia of the People's Republic of China Version 2010;1 Beijing: Chemical Industry Press; 2010.
25 Gupta AK, Charrette A. The efficacy and safety of $5{\alpha}$-reductase inhibitors in androgenetic alopecia: a network meta-analysis and benefit-risk assessment of finasteride and dutasteride. J Dermatolog Treat. 2014;25:156-61.   DOI
26 Whelchel A, Evans J, Posada J. Inhibition of ERK activation attenuates endothelin-stimulated airway smooth muscle cell proliferation. Am J Respir Cell Mol Biol. 1997;16:589-96.   DOI
27 Molavi O, Narimani F, Asiaee F, Sharifi S, Tarhriz V, Shayanfar A, Hejazi M, Lai R. Silibinin sensitizes chemo-resistant breast cancer cells to chemotherapy. Pharm Biol. 2017;55:729-39.   DOI
28 b The Pharmacopoeia Commission of PRC. Pharmacopoeia of the People's Republic of China Version 2005;1. Beijing: Chemical Industry Press; 2005