Quantitative Analysis of Puerarin and Daidzein in Domestic and Imported Puerariae Radix by High Performance Liquid Chromatography

  • Kim, Hwa-Soon (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection center) ;
  • Park, Noh-Woon (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection cente) ;
  • Kang, Hee-Gon (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection cente) ;
  • Kim, Myung-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment Kangbuk Agro-Fishery Products Inspection center)
  • Published : 2003.10.01

Abstract

This study was carried out to investigate the quality control of domestic and imported Puerariae Radix. It was analyzed by HPLC using ${\mu}$-Bondapak C$\sub$18/ column with 35% MeOH containing 1% CH$_3$3CO$_2$H system as the mobile phase at UV 254nm. Good linearity showed over the range of 10 to 200$\mu\textrm{g}$/$m\ell$(r$^2$=1) for Puerarin, and 0.5 to 10$\mu\textrm{g}$/mg(r$^2$=0.9999) for Daidzein. The average contents of Puerarin and Daidzein were 5.5${\pm}$1.2%(Domestic), 5.3${\pm}$0.7%(Imported), and 0.05${\pm}$0.02%(Domestic), 0.08${\pm}$0.02%(Imported).The average recovery rates of Puerarin and Daidzein were 101.8${\pm}$1.9% and 97.2${\pm}$0.7%, respectively.

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