• Asian Pacific Journal of Cancer Prevention
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• 제17권sup3호
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• pp.131-134
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• 2016

Breast cancer is the most common malignancy and also the second leading cause of cancer death among women and also in women that have a high mortality. Previous studies showed that magnesium (Mg) has cytotoxic effects on malignant cell lines. However, the anti-cancer effects of Mg on MCF-7 breast cancer cells are uncertain. This study was aimed at the comparison of the cytotoxic effect of Mg salt (MgCl2) and cisplatin on MCF-7 cells and fibroblasts (as normal cells). After treatment with various concentrations of MgCl2, and cisplatin as a positive control for 24 and 48 hours (h), cytotoxicity activity was measured by MTT assay. In addition, apoptosis was determined by annexin V/propidium iide assay. Both cisplatin and the MgCl2 exhibited dose-dependent cytotoxic effects in the MCF-7 cell line, although the LD50 of the Mg was significantly higher when compared to cispaltin ($40{\mu}g/ml$ vs. $20{\mu}g/ml$). Regarding annexin V/propidium results, treatment of MCF-7 cells with LD50 concentrations of cisplatin and Mg showed 59% and 44% apoptosis at 24h, respectively. Finally, the results indicated that Mg has cytotoxic effects on MCF-7 cells, but less than cisplatin as a conventional chemotherapeutic agent. However, regarding the side effects of chemotherapy drugs, it seems that Mg can be considered as a supplement for the treatment of breast cancer.

• 대한방사선기술학회지:방사선기술과학
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• 제40권3호
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• pp.407-413
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• 2017

의료 방사선은 환자의 진단 및 치료를 함에 있어 중대한 이득을 제공하지만 주변인에게 불필요한 피폭을 발생시킨다. 이에 본 연구에서는 환자와 일반인이 같은 공간 내 상주하는 다인 병실에 대해 선원항의 종류에 따른 공간선량률을 분석하고자 하였다. 실험은 몬테카를로 모의모사(MCNPX)를 이용하였으며, 선원항은 전신 뼈검사 환자와 이동형 X선 발생장치를 모사하였다. 실험결과 전신 뼈검사 환자의 측면 병상 위치에서 약 $3.46{\mu}Sv/hr$의 선량이 나타났으며, 이동형 X선 발생장치를 이용한 실험 결과, 흉부검사 시 측면 병상 위치에서 $1.47{\times}10^{-8}{\mu}Sv/irradiation$, 복부검사 시 측면 병상 위치에서 $2.97{\times}10^{-8}{\mu}Sv/irradiation$ 값이 나타났다. 이처럼 다인병실에서는 주변 환자에게 불필요한 방사선을 발생시키며, 국내의 미흡한 다인 병실의 방사선에 대한 법적인 규제 및 체계적인 차폐 방안이 마련되어져야 할 것이다.

• 한국방사선학회논문지
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• 제14권2호
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• pp.169-178
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• 2020

국민의 의료수준 향상으로 CT 검사건수는 매년 증가되고 있는 추세이며, 이에 따라 연간 의료 방사선에 의한 피폭선량 또한 증가되고 있다. 이에 본 연구에서는 CT 부위별 검사 시 노출되는 인체의 유효선량을 평가하고, 이로 인한 생애 암 귀속 위험도를 추정하고자 한다. 첫 번째로, CT 진단참고수준 가이드라인에서 제시하는 부위 중 5가지를 선정하였으며, CT 장치를 통해 각 검사 부위별로 노출되는 인체의 유효선량을 평가하였다. 두 번째로, 동일한 조건 내 ALARA-CT 프로그램을 이용하여 인체 장기 및 유효선량을 산정하였으며, 국내 DRL 수준과 비교하여 검사 프로토콜에 대한 적정성을 평가하였다. 세 번째로, CT 검사 시 노출되는 유효선량에 의한 생애 암 귀속 위험도(LAR)을 추정하였다. 그 결과, 검사 중 복부 다중시기 검사 시 21.18 mSv로 가장 높은 유효선량을 나타내었으며, 복부검사를 제외한 나머지 검사에서는 DRL 이하의 선량수준을 나타내었다. 동일한 조건 내 선량계산 프로그램을 이용한 유효선량 평가 결과, 각 검사별로 약 1.1 ~ 1.9배 이상 높은 결과를 나타내었으며, 장기선량의 경우 검사 부위에 근접한 장기일수록 산란선에 의해 높은 영향을 나타내었다. CT 검사 시 성인의 생애 암 귀속 위험도의 경우, 연령이 증가함에 따라 점차적으로 감소되는 양상을 보였으며, 성별에 따라 다소 상이한 결과를 나타내었다.

• Journal of Preventive Medicine and Public Health
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• 제41권6호
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• pp.373-379
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• 2008

Objectives ; The objective of this study was to evaluate the prevalence of atrophic gastritis and intestinal metaplasia according to gender, age and Helicobacter pylori infection in a rural population in Korea. Methods: Between April 2003 and January 2007, 713 subjects (298 men and 415 women, age range: 18-85) among the 2,161 adults who participated in a population-based survey received gastrointestinal endoscopy. All the subjects provided informed consent. Multiple biopsy specimens were evaluated for the presence of atrophic gastritis and intestinal metaplasia. The presence of Helicobacter Pylori was determined using CLO and histology testing. Results ; The age-adjusted prevalence of atrophic gastritis was 42.7% for men and 38.1% for women and the prevalence of intestinal metaplasia was 42.5% for men and 32.7% for women. The prevalence of atrophic gastritis and intestinal metaplasia increased significantly with age for both men and women (p for trend<0.001). The age-adjusted prevalence of Helicobacter pylori was similar for men (59.0%) and women (56.7%). The subjects with Helicobacter pylori infection showed a significantly higher prevalence of intestinal metaplasia (44.3%) compared with that (26.8%) of the noninfected subjects (p<0.001). However, the prevalence of atrophic gastritis was not statistically different between the Helicobacter pylori-infected subjects and the noninfected individuals. Conclusions : Our findings suggest that the prevalence of atrophic gastritis and intestinal metaplasia is higher for a Korean rural population than that for a Western population; this may be related to the high incidence of gastric cancer in Koreans. Especially, the prevalence of intestinal metaplasia was high for the subjects with Helicobacter pylori infection. The multistep process of gastric carcinogenesis and the various factors contributing to each step of this process need to be determined by conducting future follow-up studies.

• 생명과학회지
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• 제18권6호
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• pp.845-851
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• 2008

Bacillus sp. J105 strain으로부터 유도된 ${\beta}-lactamase$는 ammonium sulfate 침전, 이온 교환 칼럼 크로마토그래피, 겔 여과 등의 과정을 거쳐 SDS-PAGE에서 단일 band로 정제하였다. 정제된 효소의 분자량은 31 kDa이었으며 등전점은 7.35이었다. 효소반응의 최적 pH와 온도는 각각 5와 $40^{\circ}C$이었다. 정제 단백질의 총 아미노산 조성의 분석 결과, Gly과 Ala이 각각 14.1과 13.3 mole%로 가장 많은 아미노산 잔기를 차지하고 있었다. 정제 효소의 ampicillin을 기질로 하였을 때의 Km값은 1.33 mM이었고 Vmax값은 0.36 mM/ml이었다.

• Journal of Preventive Medicine and Public Health
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• 제36권2호
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• pp.117-124
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• 2003

Objectives : This study was performed to examine the relationship between maternal exposure to environmental tobacco smoke (ETS) and pregnancy outcomes (low birth weight or preform baby) in a prospective cohort study. Methods : We made a pregnant women's cohort, and followed the pregnancy outcomes, between May 1st 2001 and August 31st 2002. We surveyed 2,250 women who visited our hospital during their 35th gestational week, with a self-administered questionnaire. The final total of mother-infant pairs analyzed in this study was 1,712. We used a multiple logistic regression analysis to analyze the effect of maternal ETS on the incidence of preform or low birth weight, and a linear regression analysis for the birth weight and gestational age. Results : Higher exposure to ETS (>=1 hours/day) during pregnancy was more negatively associated with the gestational age and birth weight, than no exposure to ETS (no or less than 1 hour). Maternal exposure to ETS was associated with preform baby(adjusted odds ratio (AOR) 1.7; 95% confidence interval (CI) 0.9, 3.3) and low birth weight (AOR 2.3; 95% CI 0.9, 5.5). In addition, we found that maternal ETS may reduce the birth weight by 70g after adjusting for potential confounding factors. Conclusions : This study suggests that maternal exposure to ETS during pregnancy may increase the frequency of low birth weights and preform births.

• 대한지역사회영양학회지
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• 제13권1호
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• pp.80-90
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• 2008

Atopic dennatitis (AD) is one of the major public health problem. It has been reported that the prevalence of AD in children and adults are 10-20% and 1-3%, respectively. Westernization of food habits, urbanization, and environmental pollution are contributing factors toward the recent rise in prevalence. Excessive dietary restriction leads to chronic malnutrition in atopic dermatitis patients. The purpose of this study was to investigate the effects of medical nutrition therapy (MNT) on quality of diet and blood immune parameters in atopic dermatitis patients. The 19 atopic dermatitis patients (7 men and 12 women) admitted to K University Medical Center were studied. During the 12 weeks of intervention, the subjects were given MNT by a dietitian for 30-45 minutes every other week. MNT was comprised with general dietary therapy, intake of balanced meals, emphasis on n-3 fatty acid contents in foods, and food allergies. Anthropometric and dietary assessment and blood analysis were taken at baseline and after 12 weeks of MNT. After 12 weeks of MNT, the subjects' dietary qualities, including dietary diversity score (DDS), meal balance score (MBS) and dietary variety score (DVS) were significantly increased (p < 0.05). According to significantly increased intake of EPA and DHA, dietary n-6/n-3 fatty acid ratio decreased to the recommended level for the atopic dermatitis patients (p < 0.05). These changes of dietary fatty acid consumption were reflected erythrocyte fatty acid composition. After 12 weeks of MNT, serum levels of IgE and IL-4 levels were significantly decreased, however, the levers of INF-$\{gamma}$, WBC, lymphocyte and TLC were not changed. As a conclusion, the individualized MNT improved the quality of diet in atopic dermatitis patients thereby influenced RBC fatty acid composition and IgE and IL-4 levels.

• Journal of Ginseng Research
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• 제28권1호
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• pp.39-44
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• 2004

홍삼추출액의 신경성체줄기세포 성장과 생존에 미치는 영향을 분석하고자 human neuronal stem cell line인 F3 cell을 배양한 후 홍삼추출액을 여러 농도로 희석하여 MTT assay로 cell viability를 측정하였고 FACS analysis로 cell Cycle변화를 측정하였다. 특정한 농도에서는 세포가 증식되는 경향을 보였으며 농도가 증가되면서 viability가 감소되는 현상을 확인할 수 있었다. Cell cycle 분석상 세포증식시에는 5 phase 및 G2/M phase가 증가되는 경향을 보였고, viability가 감소되면서 S phase가 감소되고 G0/G1 phase 가 증가 되었다. 한편 DNA fragmentation이 cell viability감소에 따라 증가되었으나, Caspase 3 activation 또는 Bax expression과는 관련성이 적었다.

• 디지털융복합연구
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• 제12권5호
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• pp.305-310
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• 2014

본 연구는 20세 이상 여성들의 Pap 도말검사를 통한 자궁암 검진 수용도에 영향을 미치는 요인들을 규명하기 위해 시도하였다. 이를 위해 2013년 4월 22일부터 5월 24일까지 건강검진센터에 내원한 검진자들 192명을 대상으로 설문 및 면접조사를 실시하였다. 연구결과 첫째, 종교가 없는 집단에서 비검진 수용군이 45.8%로 검진 수용군의 32.3%보다 유의하게 높은 분포를 보였다($X^2=4.82$, p<.05). 둘째, 모유수유를 하는 집단의 비검진 수용군이 61.5%로 검진 수용군의 46.9%보다 유의하게 높은 분포를 하였다($X^2=0.61$, p<.05). 셋째, 타검진력은 검진을 하는 군이 검진을 하지 않는 수용군보다 4.62배 유의하게 높은 것으로 나타났다(95% Cl=1.15-9.84, P=0.006). 본 연구결과를 토대로 자궁암 Pap 도말검사 수용도에 일반인들의 부정적인 태도를 변화시킬 수 있는 더 많은 자궁암 검진 정보를 제공하는 것이 필요하다.

• 대한간호학회지
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• 제23권3호
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• pp.377-398
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• 1993

A survey of infant practices was conducted to provide information on which to base planned nursing interventions. The subjects were a convenience sample of 168 mothers visiting out - patients departments of five general hospitals and one public health center in Seoul for immunizations or treatment for common colds for their infants from two to 12 months of age. Data collection was carried out from July 8th to September 30th, 1991 using a questionnaire of 84 questions, 31 on the type of feeding, 22 on supplementary feeding and 21 on demographic infer mation. The results are summarized as follows : 1) The type of feeding was primarily artificial feed-ing (63.1%), followed by mixed feeding (22%) and breast feeding (14.9%) of the 59 mothers expecting during pregnancy to breast feed, 54. 2% changed artificial feeding and 30.5% went on to breast feed as they had expected. For mothers expecting to continue breast feeding over seven months. only one infant was being breast fed for over seven months. 2) For the 106 mothers using artificial feeding, 70. 8% hed attempted breast feeding, 64% of them for less than a month. Breast milk had been suppressed by for medication (38.7%) : 34.9% had used no specia] means. 3) The major reasons for replacing breast feeding with artificial feeding were the infants' demand for more milk (47.2% ) and insufficient supply of breast milk (49.3％). 4) Most mixed feeding was started at the age of one to three months (59.5%). Only 34.4% gave an artificial feeding after breast feeding : most (46%) alternated breast feeding with artificial feeding. On the whole, the motive for mixed feeding was the lack of breast milk (70.3%). 5) Many mothers (81.8%) were adding vitamin or mineral supplements to artificial milk and 51.5% were adding something to faciliate digestion. As for the method of sterilizing milk bottles and nipples, 56% had sterilized them together in boiling water from the beginning : 27% were just washing the bottles after boiling only once initially when measuring artificial milk powder, 31. 5% of the mothers over filled the measuring spoon rather than to the level. 6) The mother's occupation was related to her way of feeding. Mothers at home full time did more breast feeding than mothers employed outside the home. (x²=5.72, p=〈0.05). 7) Most mothers began supplementary food, from three to four months (48.8%) : 11.2% began later than seven months. Supplementary food was given between milk feedings by 67.2% of the mothers : 19.2% gave it before a milk feeding. Some mothers(26.4%) made their own supplementary food : 19.2% used ready - made supplementary food products for convenience. Recommendations for nursing interventions included : 1. Prenatal education about the advantages of breast feeding and breast care, and home visits after delivery for counselling related to breast feeding. Correct preparation of artificial feeding methods need to be taught in both pre & postnatal periods. In addition, specific education about supplementary feeding needed. 2. Further research is indicated about the Perceived lack of supply of breast milk and about the effectiveness of nursing interventions to Promote breast feeding.